Russian Journal of Infection and ImmunityRussian Journal of Infection and ImmunityИнфекция и иммунитет2220-76192313-7398SPb RAACI24510.15789/2220-7619-2014-4-347-352ORIGINAL ARTICLESОРИГИНАЛЬНЫЕ СТАТЬИTHE CHARACTERIZATION OF PROAPOPTOTIC ACTIVITY OF LIPOPOLYSACCHARIDE IN ISOLATED SUBPOPULATIONS OF HUMAN T-LYMPHOCYTESОЦЕНКА ПРОАПОПТОТИЧЕСКОЙ АКТИВНОСТИ ЛИПОПОЛИСАХАРИДА В ИЗОЛИРОВАННЫХ СУБПОПУЛЯЦИЯХ Т-ЛИМФОЦИТОВ ЧЕЛОВЕКАFilatovaE. N.ФилатоваЕ. Н.
Russian Federation

PhD (Biology), Leading Researcher, Laboratory of Molecular Biology and Biotechnology of Blokhina Research Institute of Epidemiology and Microbiology of Federal Service on Surveillance for Consumer Rights Protection and Human Welfare, Nizhny Novgorod, Russian Federation

к.б.н., ведущий научный сотрудник лаборатории молекулярной биологии и биотехнологии ФБУН НИИ эпидемиологии и микробиологии им. акад. И.Н. Блохиной Роспотребнадзора, г. Нижний Новгород, Россия

el.filatova83@gmail.comAnisenkovaE. V.АнисенковаЕ. В.
Russian Federation

Junior Researcher Laboratory of Molecular Biology and Biotechnology of Blokhina Research Institute of Epidemiology and Microbiology of Federal Service on Surveillance for Consumer Rights Protection and Human Welfare, Nizhny Novgorod, Russian Federation

младший научный сотрудник лаборатории молекулярной биологии и биотехнологии ФБУН НИИ эпидемиологии и микробиологии им. акад. И.Н. Блохиной Роспотребнадзора, г. Нижний Новгород, Россия

PresnyakovaN. B.ПресняковаН. Б.
Russian Federation

Researcher Scientist Laboratory of Molecular Biology and Biotechnology of Blokhina Research Institute of Epidemiology and Microbiology of Federal Service on Surveillance for Consumer Rights Protection and Human Welfare, Nizhny Novgorod, Russian Federation

научный сотрудник лаборатории молекулярной биологии и биотехнологии ФБУН НИИ эпидемиологии и микробиологии им. акад. И.Н. Блохиной Роспотребнадзора, г. Нижний Новгород, Россия

NeumoinaN. V.НеумоинаН. В.
Russian Federation

PhD (Medicine), Medical Director, Clinic of Inflectious Deseases of Blokhina Research Institute of Epidemiology and Microbiology of Federal Service on Surveillance for Consumer Rights Protection and Human Welfare, Nizhny Novgorod, Russian Federation

к.м.н., главный врач клиники инфекционных болезней ФБУН НИИ эпидемиологии и микробиологии им. акад. И.Н. Блохиной Роспотребнадзора, г. Нижний Новгород, Россия

SychevaT. D.СычеваТ. Д.
Russian Federation

Resident Physician, Department of Children Deseases of Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russian Federation

ординатор кафедры детских болезней ГБОУ ВПО Нижегородская государственная медицинская академия МЗ РФ, г. Нижний Новгород, Россия

UtkinO. V.УткинО. В.
Russian Federation

PhD (Biology), Head of Laboratory of Molecular Biologyand Biotechnology of Blokhina Research Institute of Epidemiology and Microbiology of Federal Service on Surveillance for Consumer Rights Protection and Human Welfare, Assistant Professor of the Department of Microbiology and Immunology of Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russian Federation

к.б.н., зав. лабораторией молекулярной биологии и биотехнологии ФБУН НИИ эпидемиологии и микробиологии им. акад. И.Н. Блохиной Роспотребнадзора, доцент кафедры микробиологии и иммунологии ГБОУ ВПО Нижегородская государственная медицинская академия МЗ РФ, г. Нижний Новгород, Россия

Blokhina Research Institute of Epidemiology and Microbiology of Federal Service on Surveillance for Consumer Rights Protection and Human Welfare, Nizhny Novgorod, Russian Federation 603950, Russian Federation, Nizhni Novgorod, Malaya Yamskaya str., 71, Nizhny Novgorod State Medical Academy. Phone: +7 (831) 469-79-46, +7 (906) 368-37-22. Fax: +7 (831) 469-79-20.ФБУН Нижегородский научно-исследовательский институт эпидемиологии и микробиологии им. акад. И.Н. Блохиной Роспотребнадзора, г. Нижний Новгород, Россия 603950, Россия, г. Нижний Новгород, ул. Малая Ямская, 71, ГОУ ВПО НижГМА МЗ РФ. Тел.: 8 (831) 469-79-46, 8 (906) 368-37-22. Факс: 8 (831) 469-79-20Blokhina Research Institute of Epidemiology and Microbiology of Federal Service on Surveillance for Consumer Rights Protection and Human Welfare, Nizhny Novgorod, Russian FederationФБУН Нижегородский научно-исследовательский институт эпидемиологии и микробиологии им. акад. И.Н. Блохиной Роспотребнадзора, г. Нижний Новгород, РоссияNizhny Novgorod State Medical Academy, Nizhny Novgorod, Russian FederationГБОУ ВПО Нижегородская государственная медицинская академия МЗ РФ, г. Нижний Новгород, Россия03122014443473520302201503022015Copyright ©; 2014, Filatova E.N., Anisenkova E.V., Presnyakova N.B., Neumoina N.V., Sycheva T.D., Utkin O.V.Copyright ©; 2014, Филатова Е.Н., Анисенкова Е.В., Преснякова Н.Б., Неумоина Н.В., Сычева Т.Д., Уткин О.В.2014Filatova E.N., Anisenkova E.V., Presnyakova N.B., Neumoina N.V., Sycheva T.D., Utkin O.V.Филатова Е.Н., Анисенкова Е.В., Преснякова Н.Б., Неумоина Н.В., Сычева Т.Д., Уткин О.В.https://creativecommons.org/licenses/by/4.0https://iimmun.ru/iimm/article/view/245

Lipopolysaccharide (LPS) is used as activator and inductor of immune cells apoptosis in case of infectious processes modeling. It is not clear if LPS is direct inductor of apoptosis in human T-cells. The subpopulations of effector and naive CD4+ T-helper and CD8+ cytotoxic T lymphocytes of human peripheric blood were isolated and cultivated in presence of LPS. It has been shown that LPS was inductor of apoptosis in studying T-cells populations.

При in vitro моделировании инфекционных процессов липополисахарид (ЛПС) применяется как активатор и индуктор апоптоза иммунокомпетентных клеток. На данный момент неясно, является ли ЛПС прямым индуктором апоптоза в Т-клетках человека. Субпопуляции эффекторных и наивных CD4+ Т-хелперов и СD8+ цитотоксических Т-лимфоцитов периферической крови человека были изолированы и культивировались раздельно в присутствии ЛПС. Показано, что ЛПС не является индуктором апоптоза в изученных субпопуляциях Т-клеток.

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