Russian Journal of Infection and ImmunityRussian Journal of Infection and ImmunityИнфекция и иммунитет2220-76192313-7398SPb RAACI194010.15789/2220-7619-NPF-1940ORIGINAL ARTICLESОРИГИНАЛЬНЫЕ СТАТЬИResearch ArticleThe non-protein fraction of embryonic stem cell secretome exerts antibacterial effects against antibiotic-resistant bacterial strainsНебелковая фракция секретома эмбриональных стволовых клеток обладает антибактериальным эффектом, в том числе против антибиотикорезистентных штаммов бактерийKaigorodovDenis G.КайгородовДенис Григорьевич
Russian Federation

Director

директор

kaigorodov_denis@rambler.ruKaigorodovaAlisa D.КайгородоваАлиса Денисовна
Russian Federation

Student

студентка

kaigorodovaalisa@mail.ruLLC Institute for Scientific Research Biotechnologies “Mitokey”ООО НИИ Биотехнологии «Митокей»Tyumen State Medical UniversityФГБОУ ВО Тюменский государственный медицинский университет Минздрава России1909202230122022126106110680405202219062022Copyright ©; 2022, Kaigorodov D.G., Kaigorodova A.D.Copyright ©; 2022, Кайгородов Д.Г., Кайгородова А.Д.2022Kaigorodov D.G., Kaigorodova A.D.Кайгородов Д.Г., Кайгородова А.Д.https://creativecommons.org/licenses/by/4.0https://iimmun.ru/iimm/article/view/1940

In recent years, it has been extremely evident to seek out for new antibacterial agents, because the burgeoning problem of antibiotic resistance and the toxicity of many antimicrobial compounds has forced scientists to turn attention to alternative approaches. Investigating stem cell secretomes, including the non-protein portion, to find new antimicrobials is a promising area in the field. We examined an effect of the non-protein portion within the embryonic stem cell secretome on various bacterial strains, including antibiotic-resistant members. The non-protein fraction of the stem cell secretome was obtained by preparative high-performance liquid chromatography. Bactericidal activity was tested against eight museum bacterial strains and 206 clinical strains by comparing the secretome-related effects on growth of bacterial cultures. The museum strains showed some dose-dependent effects at concentrations of 25–100 µg/ml. Some bactericidal activity was shown at a concentration of 100 µg/ml against the clinical strains of Gram-negative microorganisms of different species, but bacterial sensitivity to the secretome fraction varied, with growth stimulation being detected in some strains. Applying non-protein fraction of the stem cell secretome at higher concentrations of 100–1000 µg/ml showed no dose-dependent effect. The clinical strains of E. coli and P. aeruginosa were shown to have reduced bactericidal activity after 24-hour incubation. Thus, this study has shown that the non-protein fraction of the embryonic stem cell secretome exerts bactericidal effects against some bacterial strains. However, more detailed studies are needed to identify a mechanism of action and to determine the most effective dose as well as frequency of administration.

В последние годы чрезвычайно актуален поиск новых возможных антибактериальных препаратов, а проблема антибиотикорезистентности и токсичность химиопрепаратов заставляет ученых обращать внимание на альтернативные противомикробные средства. Изучение применения секретома стволовых клеток, в том числе его небелковой части, является перспективной областью современной науки. В нашем исследовании было изучено влияние небелковой части секретома эмбриональных стволовых клеток на различные штаммы микроорганизмов, в том числе антибиотикорезистентные. Небелковую часть секретома стволовых клеток получали при помощи препаративной высокоэффективной жидкостной хроматографии. Тестирование бактерицидной активности производилось в отношении 8 музейных штаммов бактерий и 206 клинических штаммов микроорганизмов методом сравнения влияния секретома на рост бактериальных культур. При первичном анализе было выявлено, что на музейных штаммах в концентрации 25–100 мкг/мл исследуемый секретом имеет некоторый дозозависимый эффект. При оценке бактерицидной активности в концентрации 100 мкг/мл в отношении клинических штаммов грамотрицательных микроорганизмов разной видовой принадлежности было показана различная чувствительность штаммов к секретому, причем у некоторых штаммов обнаружена стимуляция роста. Применение более высоких концентраций 100–1000 мкг/мл не выявило дозозависимого эффекта. При этом на клинических штаммах E. coli и P. aeruginosa было показано снижение бактерицидной активности через сутки инкубации. Таким образом, проведенное исследование показало, что небелковая фракция секретома эмбриональных стволовых клеток также обладает бактерицидным эффектом. Однако требуются более детальные исследования для выявления механизма действия и определения наиболее эффективной дозы и частоты применения.

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