Russian Journal of Infection and ImmunityRussian Journal of Infection and ImmunityИнфекция и иммунитет2220-76192313-7398SPb RAACI1821510.15789/2220-7619-CIO-18215ORIGINAL ARTICLESОРИГИНАЛЬНЫЕ СТАТЬИUnknownCOMBINED IMPACT OF STREPTOCOCCUS PYOGENES INFECTION, ANTI-STREPTOLYSIN O RESPONSE, AND IL-10/TNF-Α GENE POLYMORPHISMS ON SUSCEPTIBILITY TO RECURRENT TONSILLITISСОЧЕТАННЫЙ ЭФФЕКТ ИНФЕКЦИИ STREPTOCOCCUS PYOGENES, РЕАКЦИИ НА АНТИСТРЕПТОЛИЗИН O И ПОЛИМОРПИЗМОВ ГЕНОВ IL-10/TNF-α НА ВОСПРИИМЧИВОСТЬ К РЕЦИДИВИРУЮЩЕМУ ТОНЗИЛЛИТУMohsenRana TalibМохсенРана Талиб
Iraq

assistant professor

доцент

Ranamohsen2011@gmail.comUniversity of Anbar, IraqУниверситет Анбара, Ирак250620262404202615062026Copyright ©; , mohsen r.t.Copyright ©; , mohsen r.mohsen r.t.mohsen r.https://creativecommons.org/licenses/by/4.0https://iimmun.ru/iimm/article/view/18215

Objective: The aim of the study was to examine bacteriological profile of recurrent tonsillitis, and assess the association between Streptococcus pyogenes infection, anti-streptolysin O (ASO) levels, and cytokine gene polymorphisms (IL-10 and TNF-a).Methods: This study involved 100 participants, i.e., 50 patients with recurrent tonsillitis and 50 healthy controls. Bacteriological culture and identification were done on throat swab samples. Serum ASO was determined serologically by standard procedures. Genotype of IL -10 ( -1082 G/A) and TNF- ( -308 G/A) was done by molecular methods. These were statistically analyzed to compare the groups and evaluate correlations between the variables. Results: Bacteriological tests showed that 76% of the patient samples were culture-positive, and Streptococcus pyogenes was the most common isolate (63.2%). The level of ASO was markedly elevated in the patient group as compared to the control group with mean levels of 356.8 ± 120.5 IU/mL and 112.4 ± 48.7 IU/mL respectively (p < 0.05).Genetic analysis showed that there was increased prevalence of IL-10 AA genotype (low producer) and TNF- alpha AA genotype (high producer) in patients. Strong correlation between TNF-alpha polymorphism and disease susceptibility was found whereby there was an increased prevalence of the A allele of TNF-a in the patients. Correlation analysis revealed that TNF-alpha polymorphism was positively associated with ASO levels (r = +0.46) and IL-10 polymorphism was negatively associated with ASO levels (r = -0.39) and both these were statistically significant (p < 0.05).Conclusion: Recurrent tonsillitis is clearly linked to persistent infection with Streptococcus pyogenes, high levels of anti-streptolysin O (ASO) as well as to polymorphisms of the genes coding for cytokines. The results indicate that, the allelic variation in immune-response genes might be associated with susceptibility to recurrent tonsillitis and the immune response to streptococcal infection, because these are more common in patients. These results point to the need to consider bacteriological, immunological and genetic parameters in explaining the mechanisms of recurrent tonsillitis and could help to develop novel diagnostic and therapeutic strategies.

Цель: изучение бактериологического профиля рецидивирующего тонзиллита и оценка связи между инфекцией Streptococcus pyogenes, уровнями антистрептолизина О (АСО) и полиморфизмами генов цитокинов (IL-10 и TNF-α). Методы: В исследовании были обследованы 100 человек: 50 пациентов с рецидивирующим тонзиллитом и 50 здоровых лиц контрольной группы. Бактериологическое культивирование и идентификация проводились на материале образцов мазков из горла. Уровень АСО в сыворотке определялся серологически стандартными методами. Генотипирование IL-10 (-1082 G/A) и TNF-α (-308 G/A) проводилось молекулярными методами. Результаты были статистически проанализированы для сравнения групп и оценки корреляций между переменными. Результаты: Бактериологические тесты показали, что 76% образцов пациентов были положительными по данным культивирования, с обнаружением Streptococcus pyogenes (63,2%) как наиболее распространенного изолята. Уровень АСО был значительно повышен в группе пациентов по сравнению с контролем, при средних значениях 356,8 ± 120,5 МЕ/мл и 112,4 ± 48,7 МЕ/мл соответственно (p < 0,05). Генетический анализ обнаружил повышенную распространенность генотипа IL-10 AA (низкий уровень продукции) и генотипа TNF-α AA (высокий уровень продукции) у пациентов. Отмечена сильная корреляция между полиморфизмом TNF-α (повышенная распространенность аллеля А) и предрасположенностью к заболеванию у пациентов. Корреляционный анализ показал достоверную (p < 0,05) положительную и отрицательную корреляцию между полиморфизмом TNF-α с уровнями АСО (r = +0,46), а также полиморфизмом IL-10 с уровнями АСО (r = -0,39) соответственно. Вывод: Рецидивирующий тонзиллит выраженно сцеплен с персистирующей инфекцией Streptococcus pyogenes, высокими уровнями антистрептолизина O (АСО), а также с полиморфизмами генов, кодирующих цитокины. Результаты указывают на то, что аллельная вариация в генах иммунного ответа может быть связана с предрасположенностью к рецидивирующему тонзиллиту и иммунным ответом на стрептококковую инфекцию, как это чаще обнаруживается у пациентов. Полученные результаты указывают на необходимость учета бактериологических, иммунологических и генетических параметров при оценке механизмов рецидивирующего тонзиллита и могут способствовать разработке новых диагностических и терапевтических подходов.

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