Russian Journal of Infection and ImmunityRussian Journal of Infection and ImmunityИнфекция и иммунитет2220-76192313-7398SPb RAACI176110.15789/2220-7619-RBT-1761ORIGINAL ARTICLESОРИГИНАЛЬНЫЕ СТАТЬИRelationship between type III secretion toxins, biofilm formation, and antibiotic resistance in clinical Pseudomonas aeruginosa isolatesВзаимосвязь между токсинами III типа секреции, образованием биопленки и антибиотической резистентностью в клинических изолятах Pseudomonas aeruginosaDerakhshanS.ДеракшанС.
Iran, Islamic Republic of

Safoura Derakhshan - PhD, Liver and Digestive Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences.

Sanandaj.

Phone: +98 87 33668504.

Дерахшан Сафура – кандидат наук, Центр исследования печени и органов пищеварения, Курдский университет медицинских наук.

Сенендедж.

Тел.: +98 87 33668504.

s.derakhshan@muk.ac.irRezaeeA.МохаммадиШ.
Iran, Islamic Republic of

Ali Rezaee - MSc, Student Research Committee, Kurdistan University of Medical Sciences.

Sanandaj.

Кандидат наук, Исследовательский центр зоонозов, Научно-исследовательский институт развития здравоохранения, Курдский университет медицинских наук.

Сенендедж.

alirezaei2610@gmail.comMohammadiSh.РезайиА.
Iran, Islamic Republic of

Shadieh Mohammadi - PhD, Zoonoses Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences.

Sanandaj.

Магистр наук, студенческий научно-исследовательский комитет, Курдский университет медицинских наук.

Сенендедж.

shadiehmohammadi@yahoo.comKurdistan University of Medical SciencesКурдский университет медицинских наук05012022116107510823006202120092021Copyright ©; 2021, Derakhshan S., Rezaee A., Mohammadi S.Copyright ©; 2021, Деракшан С., Мохаммади Ш., Резайи А.2021Derakhshan S., Rezaee A., Mohammadi S.Деракшан С., Мохаммади Ш., Резайи А.https://creativecommons.org/licenses/by/4.0https://iimmun.ru/iimm/article/view/1761

Background and aim. Pseudomonas aeruginosa is considered as a notorious pathogen due to its multidrug resistance and life threatening infections. We investigated the relationship between type III secretion toxins, biofilm formation, and antibiotic resistance among clinical P. aeruginosa isolates. Methods. A total of 70 genetically distinct clinical P. aeruginosa isolates were characterized for antibiotic resistance by disk diffusion assay. Biofilm formation was evaluated by microtiter plate method and presence of four exo genes (exoS, exoU, exoT and exoY) was investigated by PCR. A p-value < 0.05 was regarded statistically significant. Results. The most effective antibiotics were Meropenem and Piperacillin. Multidrug resistance was more prevalent in the ciprofloxacin-resistant isolates than in the susceptible isolates. The most frequently identified exo was exoS (37.1%). Genotype exoS/exoT was found in 4 isolates, while genotype exoU/exoT was not found. Prevalence of exoS was generally higher in the susceptible isolates than in the resistant isolates. A significant association was found between the formation of strong biofilm and resistance to antibiotics (p < 0.05). Prevalence of exoY and exoU was higher in the non-strong biofilm producers compared to the strong biofilm producers. Conclusion. Our study revealed formation of strong biofilm along with antibiotic resistance and the presence of exo genes in P. aeruginosa isolates. Knowledge of virulence gene profiles and biofilm formation may be useful in deciding appropriate treatment.

Актуальность и цель. Pseudomonas aeruginosa считается опасным патогеном из-за своей множественной лекарственной устойчивости и вызываемых им инфекций, представляющих угрозу для жизни. Мы исследовали взаимосвязь между токсинами секреции III типа, образованием биопленок и устойчивостью к антибиотикам среди клинических изолятов P. aeruginosa. Методы. Диско-диффузионный анализ был использован для оценки устойчивости к антибиотикам у 70 генетически различных клинических изолятов P. aeruginosa. Образование биопленок оценивали в микротитрационном планшете, а наличие четырех экзогенов (exoS, exoU, exoT и exoY) исследовали с помощью полимеразной цепной реакции. Значение p < 0,05 считалось статистически значимым. Результаты. Наиболее эффективными антибиотиками оказались меропенем и пиперациллин. Множественная лекарственная устойчивость была более распространена у устойчивых, чем у чувствительных к ципрофлоксацину изолятов. Наиболее часто выявляемым экзоном был exoS (37,1%). Генотип exoS/exoT обнаружен у 4 изолятов, генотип exoU/exoT не выявлялся. Распространенность exoS, как правило, была выше у чувствительных изолятов, чем у устойчивых. Была обнаружена достоверная связь между образованием прочной биопленки и устойчивостью к антибиотикам (p < 0,05). Распространенность exoY и exoU была выше у продуцентов непрочных биопленок, чем у продуцентов прочных биопленок. Заключение. Наше исследование, наряду с устойчивостью к антибиотикам и наличием экзогенов, выявило у изолятов P. aeruginosa способность к формированию прочной биопленки. Знание профиля генов вирулентности и образования биопленок может быть полезно при выборе соответствующего лечения.

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