Russian Journal of Infection and ImmunityRussian Journal of Infection and ImmunityИнфекция и иммунитет2220-76192313-7398SPb RAACI1758210.15789/2220-7619-BON-17582ORIGINAL ARTICLESОРИГИНАЛЬНЫЕ СТАТЬИResearch ArticleBiosynthesis of novel MnO2 nanocapsules via C. spinosa extract and honeybee-derived chitosan: exploring antibacterial and anticancer propertiesБиосинтез новых нанокапсул MnO2 с помощью экстракта C. Spinosa и хитозана медоносной пчелы: изучение антибактериальных и противораковых свойствElharrifMohamed GamalЭльхаррифМохамед Гамаль
Saudi Arabia

PhD, Department of Basic Medical Sciences, College of Medicine

PhD, кафедра фундаментальных медицинских наук, Медицинский колледж

al_harrif@yahoo.comHassanN. A.ХасанН. А.
Egypt

PhD, Synthetic Unit, Department of Photochemistry

PhD, отдел синтеза, кафедра фотохимии

al_harrif@yahoo.comSharafM.ШарафМ.
Egypt

PhD, Department of Biochemistry and Molecular Biology

PhD, кафедра биохимии и молекулярной биологии

al_harrif@yahoo.comShaqra UniversityУниверситет ШакраChemical Industries Research Institute, National Research CentreНаучно-исследовательский институт химической промышленности, Национальный исследовательский центрAL-Azhar UniversityУниверситет Аль-АзхарOcean University of ChinaОкеанский университет Китая1505202421122024145100210162901202406042024Copyright ©; 2024, Elharrif M.Г., Hassan N.A., Sharaf M.Copyright ©; 2024, Эльхарриф М.G., Хасан Н.А., Шараф М.2024Elharrif M.Г., Hassan N.A., Sharaf M.Эльхарриф М.G., Хасан Н.А., Шараф М.https://creativecommons.org/licenses/by/4.0https://iimmun.ru/iimm/article/view/17582

This investigation delves into the integration of Capparis spinosa extract (CSLe) onto manganese dioxide nanoparticles (MnO2NPs) and chitosan derived from honeybees (CSH) in a nanostructured configuration. The resultant nanocomposites, namely CSLe@MnO2NPs and CSH/CSLe@MnO2NPs, underwent thorough characterization through various analytical techniques. UV-Vis spectroscopy unveiled distinctive features, such as ligand-to-metal charge transfer and photoluminescence, affirming the successful chitosan-functionalization of the MnO2NPs, thereby differentiating them from their pristine counterparts. FTIR spectra corroborated the binding of chitosan and identified crucial molecular functional groups. SEM-EDX analysis revealed the morphological properties, addressing non-uniform sizes in the as-calcined MnO2NPs by the uniform coating of CSH on CSLe@MnO2NPs, while EDX confirmed the presence of essential elements. TEM and SAED provided insights into the spherical morphology, crystalline structure, and lattice planes of these nanoparticles. Size distribution measurements highlighted distinctions between CSLe@MnO2NPs and CSH/CSLe@MnO2NPs. The nanomaterials underwent evaluation for their antimicrobial properties against a spectrum of Gram-negative and Gram-positive bacterial strains, with CSH/CSLe@MnO2NPs exhibiting the highest bactericidal activity. Additionally, they demonstrated low minimum inhibitory concentration (MIC) values, especially against S. aureus (MIC as low as 12.5 µg/ml). Their efficacy extended to anti-biofilm formation, significantly diminishing biofilm development in a dose-dependent manner, a pivotal factor in addressing biofilm-related infections. The study also scrutinized their cytotoxicity against normal Vero and PC3 prostate cancer cells, revealing potential anticancer properties. Dose-dependent reductions in cell viability were observed for both normal and cancer cells. In conclusion, these findings underscore the versatility and promise of CSH/CSLe@MnO2NPs in diverse biomedical applications, including antibacterial, anti-biofilm, and anticancer therapies.

Настоящее исследование посвящено описанию нанесения экстракта Capparis spinosa (CSLE) на наночастицы диоксида марганца (MnO2NP) и хитозан медоносных пчел (CSH) в наноструктурированной конфигурации. Полученные нанокомпозиты, а именно CSLE@MnO2NPS и CSH/CSLE@MnO2NPS, были тщательно охарактеризованы с помощью различных аналитических методов. Спектроскопия в УФ- и видимой области обнаружила отличительные особенности, такие как перенос заряда «лиганд–металл» и фотолюминесценцию, подтверждая успешную функционализацию хитозана на MnO2NP, тем самым дифференцируя их от соответствующих интактных аналогов. Спектры инфракрасной спектроскопии с преобразованием Фурье (ИКФС) подтвердили связывание хитозана и идентифицировали ключевые молекулярные функциональные группы. Анализ с помощью способа линейного сканирования SEM-EDX выявил морфологические свойства, касающиеся неравномерных размеров кальцинированных MnO2NP с помощью равномерного покрытия CSH на CSLE@MnO2NP, в то время как энергодисперсионный рентгеноспектральный микроанализ (EDX) подтвердил наличие необходимых элементов. Просвечивающая электронная микроскопия (TEM) и электронная дифракция на отдельных участках (SAED) дали представление о сферической морфологии, кристаллической структуре и кристаллической плоскости таких наночастиц. Измерения распределения по размерам выявили различия между CSLe@MnO2NPs и CSH/CSLe@MnO2NPs. Наноматериалы прошли оценку на антимикробные свойства в отношении различных грамотрицательных и грамположительных бактериальных штаммов, с максимальной бактерицидной активностью у CSH/CSLe@MnO2NPs. Кроме того, минимальная ингибирующая концентрация (MIC), особенно против S. aureus (MIC не более 12,5 мкг/мл) описана при низких значениях. Их эффективность также распространялась на формирование антибиопленки, достоверно дозозависимо снижая образование биопленки как ключевого фактора в отношении инфекций, связанных с биопленкой. Также тщательно изучена цитотоксичность соединений в отношении нормальных клеток Vero и клеток рака предстательной железы PC3, выявившая дозозависимое снижение жизнеспособности клеток обеих линий. Полученные результаты подчеркивают универсальность и перспективность CSH/CSLE@MnO2NP при использовании в различных биомедицинских целях, включая антибактериальное действие, подавление синтеза антибиопленки и противоопухолевую терапию.

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