Russian Journal of Infection and ImmunityRussian Journal of Infection and ImmunityИнфекция и иммунитет2220-76192313-7398SPb RAACI12310.15789/2220-7619-2013-1-49-58ORIGINAL ARTICLESОРИГИНАЛЬНЫЕ СТАТЬИCYTOKINES AND CHEMOKINES IN THE BLOOD PLASMA OF PATIENTS WITH CHRONIC HEPATITIS CПРОФИЛЬ ЦИТОКИНОВ И ХЕМОКИНОВ В ПЛАЗМЕ КРОВИ ПАЦИЕНТОВ С ХРОНИЧЕСКИМ ГЕПАТИТОМ СSysoevK. A.СысоевК. А.
Russian Federation

к.м.н., старший научный сотрудник НМЦ по молекулярной медицине МЗ РФ

197022, Санкт-Петербург, ул. Льва Толстого, 6/8

ksyssoev@mail.ruChukhlovinA. V.ЧухловинА. Б.
Russian Federation
ksyssoev@mail.ruShakhmanovD. M.ШахмановД. М.
Russian Federation
ksyssoev@mail.ruZhdanovK. V.ЖдановК. В.
Russian Federation
ksyssoev@mail.ruTotolianAreg A.ТотолянАрег А.
Russian Federation
ksyssoev@mail.ruСанкт-Петербургский государственный медицинский университет им. акад. И.П. Павлова, Санкт-ПетербургВоенно-медицинская академия им. С.М. Кирова, Санкт-ПетербургФБУН НИИ эпидемиологии и микробиологии имени Пастера, Санкт-Петербург040120133149580707201407072014Copyright ©; 2014, Sysoev K.A., Chukhlovin A.V., Shakhmanov D.M., Zhdanov K.V., Totolian A.A.Copyright ©; 2014, Сысоев К.А., Чухловин А.Б., Шахманов Д.М., Жданов К.В., Тотолян А.А.2014Sysoev K.A., Chukhlovin A.V., Shakhmanov D.M., Zhdanov K.V., Totolian A.A.Сысоев К.А., Чухловин А.Б., Шахманов Д.М., Жданов К.В., Тотолян А.А.https://creativecommons.org/licenses/by/4.0https://iimmun.ru/iimm/article/view/123

Abstract. Pathogenesis of chronic hepatitis C (CHC) remains to be determined. Mechanisms of liver parenchyma damage in patients with CHC are complex and different. Cytokines play the role of intermediaries in the process of fibrosis development and chronic inflammation. In the present study levels of 27 cytokines in the blood plasma of 14 patients with CHC were tested using multiplex analysis. The liver biopsy was performed in all patients to define the activity of inflammation (histological activity index) and the degree of fibrosis. Nineteen samples of blood plasma obtained from healthy individuals were served as a control group in this study. The following cytokines were measured: IL-1β, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 (p70), IL-13, IL-15, IL-17, eotaxin, FGF-2, G-CSF, GM-CSF, IFNγ, IP-10, MCP-1, MIP-1α, MIP-1β, RANTES, PDGF-BB, TNFα and VEGF. In patients with CHC elevated levels of plasma IL-1ra, IL-6, IL-7, IFNγ, IL-12 (p70), IL-4, IL-9, IL-8, IP-10, eotaxin, MCP-1, MIP-1β, TNFα, G-CSF and GM-CSF were found in compare with the control group. At the same time levels of FGF-2 and PDGF-BB were reduced in patients with CHC in compare with controls. Differences in the production of IL-1ra, IL-6, IL-7, IFNγ, IL-12 (p70), IL-4, IL-9, IL-8, IP-10, eotaxin, MCP-1, MIP-1β, TNFα, G-CSF and GM-CSF were depend on the genotype of HCV (3a or 1b), histological activity index in liver tissue and the degree of liver fibrosis. The revealed changes of cytokine production in patients with CHC characterize different orientation of regulatory violations confirming that CHC is an immunopathological process.

Резюме. Патогенез хронического гепатита С (ХГС) изучен недостаточно. Механизмы повреждения печеночной паренхимы при ХГС отличаются сложностью и разнообразием. Цитокинам отводится роль посредников в процессах фиброза и хронического воспаления. В настоящей работе с помощью мультиплексного анализа проведено исследование содержания 27 цитокинов в плазме крови 14 пациентов, страдающих ХГС. Пациентам была проведена биопсия печени, при которой определялась активность воспаления (индекс гистологической активности) и степень фиброза. В качестве контрольной группы изучены образцы плазмы крови 19 практически здоровых лиц. Определялись следующие цитокины: IL-1β, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12(p70), IL-13, IL-15, IL-17, эотаксин, FGF-2, G-CSF, GM-CSF, IFNγ, IP-10, MCP-1, MIP-1α, MIP-1β, RANTES, PDGF-BB, TNFα и VEGF. У пациентов с ХГС было выявлено повышенное по сравнению с контрольной группой содержание в плазме крови IL-1ra, IL-6, IL-7, IFNγ, IL-12(p70), IL-4, IL-9, IL-8, IP-10, эотаксина, MCP-1, MIP-1β, TNFα, G-CSF и GM-CSF. Уровни FGF-2 и PDGF-BB у пациентов с ХГС были снижены по сравнению с контролем. В зависимости от генотипа вируса гепатита С (3a и 1b), индекса гистологической активности в ткани печени и степени фиброза были выявлены различия в продукции IL-1ra, IL-6, IL-7, IFNγ, IL-12(p70), IL-4, IL-9, IL-8, IP-10, эотаксина, MCP-1, MIP-1β, TNFα, G-CSF и GM-CSF. Выявленные изменения уровня цитокинов в плазме крови у пациентов с ХГС характеризуют различную направленность регуляторных нарушений, что характеризует ХГС как иммунопатологический процесс.

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