EXPERIMENTAL STUDY OF THE EFFECTIVENESS OF THE JOINT THERAPEUTIC ACTION OF LACTOGLOBULIN AND SALLMONELLA BACTERIOPHAGE

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Abstract

The wide spread of drug-resistant forms of the pathogens, complicating prevention and treatment of acute intestinal infections, determines the feasibility of using bacteriophages and immune lactoglobulins for antibacterial therapy of these diseases. The aim of this study was to investigate the antibacterial efficacy of the joint use of immune lactoglobulin and Salmonella bacteriophage in the treatment of experimental salmonellosis in mice. The experimental Salmonella infection was reproduced by means of a single intragastric administration of 2LD50 of daily agar culture of S.typhimurium. As a result, the development of Salmonella infection lasting up to 14 days was noted. The animals were divided into four groups according to the pattern of the treatment. The following preparations were used for the treatment of animals: «Lactoglobulin against opportunistic bacteria and Salmonella» dry cow, «Lactoglobulin» liquid, bacteriophage of Salmonella ABCDE groups, liquid. The preparations were daily administered to mice orally after the infection for 7 days. In the study of the joint therapeutic effect of the lactoglobulin and bacteriophage the special attention was paid to the terms of elimination of the pathogen from animal organs. Analysis of the data showed that in all four groups a positive dynamics of excretion of the pathogen of mice was revealed. In groups of animals that were treated with both drugs, the elimination of Salmonella out of organs occurred 1.5 times faster than in the control groups of mice. At the same time in the experimental groups on the third day of treatment with preparations the statistically significant decrease in the number of S.typhimurium in the contents of the large intestine of animal was revealed. The study found that immune lactoglobulin used simultaneously with Salmonella phage for 7 days reduced the severity and duration of the disease course and blocked the further development of the infectious process, promoted the efficient and rapid cleansing of animals organism of the pathogen, increased the survival of mice. The obtained data suggest the applicability of joint use of immune lactoglobulin preparations and bacteriophages in the treatment of acute intestinal infections caused by antibiotic-resistant strains of Salmonella.

About the authors

N. V. Aleksanina

Rostov Research Institute of Microbiology and Parasitology

Author for correspondence.
Email: nataly10_09@mail.ru

Natalya V. Aleksanina - PhD (Biology), Senior Researcher, Laboratory of Sanitary Microbiology of Water Bodies and Human Microbial Ecology.

344010,Rostov-on-Don, Gazetny per., 119. Phone: +7 (863) 234-70-55 (office); +7 961 288-02-59 (mobile)

Russian Federation

O. V. Moiseyeva

Rostov Research Institute of Microbiology and Parasitology

Email: fake@neicon.ru

Junior Researcher, Laboratory of Sanitary Microbiology of Water Bodies and Human Microbial Ecology

Russian Federation

References

  1. А лексанина Н.В., Соболева С.В. Микробиоценоз толстого кишечника детей с диарейными заболеваниями в процессе лечения лактоглобулином против условно-патогенных бактерий и сальмонелл // Актуальные вопросы инфекционной патологии. Ростов-на-Дону, 2009. С. 302–305. [Aleksanina N.V., Soboleva S.V. Microbiocenosis of children’s colon with diarrheal diseases in the process of treatment with lactoglobulin against opportunistric bacteria and Salmonella. Actual issues of infectious pathology. Rostov-on-Don, 2009, pp. 302–305.]
  2. Воротынцева Н.В., Мазанкова Л.Н. Острые кишечные инфекции у детей. М.: Медицина, 2001. 480 с. [Vorotyntseva N.V., Mazankova L.N. Ostrye kishechnye infektsii u detei [Acute intestinal infections in children]. Moscow: Medicine, 2001, 480 p.]
  3. Дроздова О.М., Брусина Е.Б. Применение бактериофагов в эпидемиологической практике: взгляд через столетие // Эпидемиология и инфекционные болезни. 2010. № 5. С. 20–24. [Drozdova O.M., Brusina E.B. Use of bacteriophages in epidemiological practice: a view after a century. Epidemiologiya i infektsionnye bolezni = Epidemiology and Infectious Diseases, 2010, no. 5, pp. 20 –24. (In Russ.)]
  4. О профилактике острых кишечных инфекций: Постановление Главного государственного санитарного врача Российской Федерации от 19 марта 2010 г. № 21. [On the prevention of acute intestinal infections: Resolution Chief State Sanitary Officer Russian Federation of 19 March 2010 no. 21]. URL: docs.cntd.ru/document/902206076 (15.06.2017)
  5. Онищенко Г.Г. Заболеваемость острыми кишечными инфекциями в Российской Федерации // Иммунология. 2008. № 1. С. 18–23. [Onishchenko G.G. The incidence of acute intestinal infections in Russian Federation. Immunologiya = Immunology, 2008, no. 1, pp. 18–23. (In Russ.)]
  6. Соболева С.В. Лактоглобулины — препараты нового поколения для лечения и профилактики острых кишечных инфекций и дисбактериозов // Актуальные вопросы инфекционной патологии. Ростов-на-Дону, 2009. С. 251–255. [Soboleva S.V. Lactoglobulins — a new generations of drugs for treatment and prevention of acute intestinal infections and disbacteriosis. Actual issues of infectious pathology. Rostov-on-Don, 2009, pp. 251–255.]
  7. Урсова Н.И., Минухина А.М. Бактериофаги. К линико-иммунологическая эффективность иммунологических препаратов // Под ред. М.П. Костинова, Н.В. Медуницина. М., 2008. 256 с. [Ursova N.I., Minuhina A.M. Bakteriofagi. Kliniko-immunologicheskaya effektivnost’ immunologicheskikh preparatov. Pod red. M.P. Kostinova, N.V. Medunitsina [Bacteriophages. Clinical and Immunological Efficacy of immunological preparations. Eds.: M.P. Kostinov, N.V. Medunitsin]. Moscow, 2008, 256 p.].

Copyright (c) 2017 Aleksanina N.V., Moiseyeva O.V.

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