IN VITRO CD4+ T CELL RESPONSE TO HELICOBACTER PYLORI ANTIGENS PRESENTATION IN PATIENTS WITH H. PYLORI INFECTION AND GASTRITIS WITH AND WITHOUT GASTRIC MUCOSA EROSION



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Abstract

Introduction. H. pylori is a widespread microbe able to long-term persist on gastric mucosa and may cause gastritis, peptic ulcers, and malignant neoplasms of the stomach. It is assumed that the clinical manifestations of the infection depend on pathogen virulence and host response, but the characteristics of the immune response to H. pylori in various morphological forms of gastric lesions have not been sufficiently studied. The aim of the study was to identify peripheral blood antigen-specific CD4+ T cells during H. pylori infection and to evaluate the T cell activation response to H. pylori antigens in infected patients with erosive and non-erosive gastritis. Materials and methods. Peripheral blood monocytes and lymphocytes were isolated from study subjects. The monocytes were incubated with or without H. pylori. Next, lymphocytes were stimulated with syngeneic monocytes, and the CD4+ T cell response to antigens was assessed by expression of activation markers OX40, CD69, and CD25. Results. Antigen-specific CD4+ T cells were easily detected in the blood of patients with non-erosive gastritis by identifying antigen-induced expression of the activation marker OX40. In H. pylori-associated erosive gastritis, stimulation of CD4+ T cells with antigens did not increase OX40 expression and decreased CD69 expression. Evaluation of CD25 expression in combination with CD69 or OX40, as well as analyzing co-expression of these molecules in groups of large and small lymphocytes, allowed to identify antigen-induced changes in the CD4+ T cell phenotype in patients with erosive gastritis. Hence, antigen-induced increase in the number of CD25lowCD69low cells within both large and small CD4+ T lymphocytes most reliably mirrors T-helper activation in these patients. The antigen-induced increase in the number of CD25hiCD69-/low and CD25hiCD69hi large lymphocytes was more pronounced. However, such cells with high CD25 expression levels can be either due to activated T helpers or inducible regulatory T cells. Conclusion. Using an in vitro antigen presentation model, CD4+ T cells specific to H. pylori antigens were detected in peripheral blood samples from patients with H. pylori-associated gastritis. The characteristics of antigen-specific T-cell response in this model varied across different morphological forms of gastric lesions.

About the authors

Vladimir Talayev

Academician I.N. Blokhina Nizhny Novgorod Scientific Research Institute of Epidemiology and Microbiology of Russian Federal Consumer Rights Protection and Human Health Control Service

Email: talaev@inbox.ru
ORCID iD: 0000-0003-1993-0622
SPIN-code: 5958-4703
Scopus Author ID: 8547169700

DSc (Medicine), Professor, Head of the Laboratory of Cellular Immunology

Russian Federation, 603950, BOX 145, M. Yamskaya str. 71, Nizhny Novgorod, Russia

Elena V. Kurkova

Academician I.N. Blokhina Nizhny Novgorod Scientific Research Institute of Epidemiology and Microbiology of Russian Federal Consumer Rights Protection and Human Health Control Service

Email: el2v@mail.ru
ORCID iD: 0000-0003-1801-9693
SPIN-code: 6615-7674
Scopus Author ID: 56841316700

PhD (Biology), Senior Researcher, Laboratory of Cellular Immunology

Russian Federation, 603950, BOX 145, M. Yamskaya str. 71, Nizhny Novgorod, Russia

Maria V. Svetlova

Academician I.N. Blokhina Nizhny Novgorod Scientific Research Institute of Epidemiology and Microbiology of Russian Federal Consumer Rights Protection and Human Health Control Service

Email: marya.talaeva@yandex.ru
ORCID iD: 0000-0003-4097-6780
SPIN-code: 8340-7583
Scopus Author ID: 36471139400

PhD (Biology), Senior Researcher, Laboratory of Cellular Immunology

Russian Federation, 603950, BOX 145, M. Yamskaya str. 71, Nizhny Novgorod, Russia

Olga N. Babaykina

Academician I.N. Blokhina Nizhny Novgorod Scientific Research Institute of Epidemiology and Microbiology of Russian Federal Consumer Rights Protection and Human Health Control Service

Email: olga_babaykina@inbox.ru
ORCID iD: 0000-0003-4527-6134
SPIN-code: 9438-9974
Scopus Author ID: 8547169900

PhD (Medicine), Senior Researcher, Laboratory of Cellular Immunology

Russian Federation, 603950, BOX 145, M. Yamskaya str. 71, Nizhny Novgorod, Russia

Natalia V. Neumoina

Academician I.N. Blokhina Nizhny Novgorod Scientific Research Institute of Epidemiology and Microbiology of Russian Federal Consumer Rights Protection and Human Health Control Service

Email: tak1510@yandex.ru
ORCID iD: 0000-0002-1394-3484

PhD (Medicine), Head Physician of the Infectious Diseases Clinic

Russian Federation, 603950, Nizhniy Novgorod, Malaya Yamskaya str., 71

Ksenia M. Perfilova

Academician I.N. Blokhina Nizhny Novgorod Scientific Research Institute of Epidemiology and Microbiology of Russian Federal Consumer Rights Protection and Human Health Control Service

Author for correspondence.
Email: kperfilova@yandex.ru
ORCID iD: 0000-0001-6395-9014

PhD (Medicine), Deputy Head Physician of the Infectious Diseases Clinic

Russian Federation, 603950, Nizhniy Novgorod, Malaya Yamskaya str., 71

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