IMMUNE SYSTEM INDICATORS IN PATIENTS WITH ACUTE OPISTHORCHIASIS



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Abstract

The Ob-Irtyshsky focus, which includes the Tyumen region is largest among Opisthorhis felineus invasion sites. The high intensity of invasion spreading in the outbreak underlies a high risk of infection often occurring with acute stage for the residents of the Tyumen region. The aim of this study was to evaluate the immunological parameters in patients with acute opisthorchiasis compared to healthy subjects. Ten patients at the clinic of the Tyumen Research Institute of Regional Infectious Pathology were examined with a diagnosis of acute opisthorchiasis. Immunological examination included lymphocyte phenotyping by flow cytometry. The concentration of immunoglobulins M, G, A, and E and cytokines IFN-γ, IL-4, IL-8, IL-10 in blood serum was quantitated by enzyme immunoassay. Neutrophil absorbance was investigated in latex particle test (d 1 μm). Neutrophil myeloperoxidase enzyme activity was measured spectrophotometrically, concentration of circulating immune complexes – by precipitation with PEG-6000 solution. In patients with acute opisthorchiasis, increased count of leukocytes, eosinophils, monocytes was detected; increased neutrophil scavenging and enzymatic activity; increased absolute number of CD3+ cells, absolute number of T-lymphocytes with CD3+HLA DR+ marker, relative and absolute number of CD4+ cells were found as well. The concentration of total immunoglobulins G, A, M as well as circulating immune complexes and pro-inflammatory cytokine IL-8 were increased. A positive correlation between IL-8 levels and count of eosinophils, monocytes and percentage of natural killers was revealed in patients but not in control group. Patients have decreased levels of IFN-γ and the cytokine IL-10. The level of IL-4 did not differ between patients with opisthorchiasis vs. control subjects. The correlation between IL-4 level and circulating immune complexes, count of eosinophils and natural killer cells was found in patients but not in control group. Thus, patients with acute opisthorchiasis were characterized by activated innate immune response as well as cellular and humoral arms of the adaptive immune response.

About the authors

Svetlana Andreyevna Grigorieva

Tyumen Region Infection Pathology Research Institute

Email: grigorjeva.svetlana66@yandex.ru
ORCID iD: 0000-0002-5391-7256
SPIN-code: 3285-3448

Research Associate

Russian Federation, 625026, Russia, Tyumen, Republic Street, 147

Xenia Borisovna Stepanova

Tyumen Region Infection Pathology Research Institute

Email: info@Tniikip.rospotrebnadzor.ru
ORCID iD: 0000-0002-5420-0919
SPIN-code: 1967-8499
Scopus Author ID: 23767533600
ResearcherId: -

Doctor of Medical Sciences, Director

Russian Federation, 625026, Russia, Tyumen, Republic Street, 47

Tatyana Fiodorovna Stepanova

Tyumen Region Infection Pathology Research Institute

Email: info@Tniikip.rospotrebnadzor.ru
ORCID iD: 0000-0002-6289-6274
SPIN-code: 1212-5143
Scopus Author ID: 7007079186
ResearcherId: AAG-2889-2020

Doctor of Medical Sciences, Professor, Chief Researcher

Russian Federation, 625026, Russia, Tyumen, Republic Street, 147

Lyubov Vladimirovna Kurlaeva

Tyumen Region Infection Pathology Research Institute

Email: info@Tniikip.rospotrebnadzor.ru
ORCID iD: 0000-0003-4389-7199
SPIN-code: 1544-4369

Junior Research Fellow at the Department of Clinical and Experimental Immunology

Russian Federation, 625026, Russia, Tyumen, Republic Street, 147

Elena Ivanovna Swede

Tyumen Region Infection Pathology Research Institute

Email: info@Tniikip.rospotrebnadzor.ru

Russian Federation, 625026, Russia, Tyumen, Republic Street, 147

Julia Vyacheslavovna Chirko

Tyumen Region Infection Pathology Research Institute

Author for correspondence.
Email: info@Tniikip.rospotrebnadzor.ru

Junior Researcher at the Clinic and Therapy Group for Biohelminthiasis

Russian Federation, 625026, Russia, Tyumen, Republic Street, 147

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Copyright (c) Grigorieva S.A., Stepanova X.B., Stepanova T.F., Kurlaeva L.V., Swede E.I., Chirko J.V.

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