LACTOBACCILUS SPP. AS MICROBIAL MARKERS FOR ASSESSING THE STATE OF HUMAN MICROSYMBIOCENOSES

The human body and its microbiota are closely related to each other and are in dynamic equilibrium, being a single ecological system. The study involved 59 volunteers divided into 2 age groups: group 1 - from 20 to 39 years old and group 2 - from 40 to 59 years old, with a history of inflammatory diseases of the oral mucosa and periodontal tissues. According to the survey results, a higher risk of developing oral dysbiosis followed by an inflammatory process was revealed in group 2. In the conducted study, autochthonous species such as L.plantarum, L. paracasei, and L.fermentum prevailed in the digestive tract of the Tyumen residents. The authors proved that a high degree of seeding of 10⁷ CFU/ml of L. paracasei in group 1 individuals is a marker for normobiota, whereas L. fermentum and L. rhamnosus are indicators of dysbiosis in this biotope. No such pattern was found in group 2 patients. Low rates of Lactobaccilus spp. up to 10⁴ CFU/ml indicates an imbalanced microbiota composition and indicates health issues. In inflammatory diseases of the oral cavity, a specific lactobacillus deficiency was observed in all subjects, regardless of age. In dental lesions of the oral cavity, two strains were found to prevail: L. fermentum and L. plantarum. The isolation of these lactobacillus strains in the studied pathological processes proves their indicator etiological significance. Presumably, the species diversity of Lactobaccilus spp. in patient oral cavity was influenced by comorbidities in the anamnesis. The study of the species composition for bacterial and fungal associations in the gastrointestinal tract and oral cavity allowed to determine that the presence of certain species of Lactobaccilus spp. correlates with detected pathogenic and opportunistic microorganisms. It is verified that, regardless of the biotope, L. fermentum species is as an indicator of the dysbiotic microsymbiocenosis.