CORYNEBACTERIUM SPP.: GENOMIC ANALYSIS AND ROLE IN SHAPING SKIN MICROBIOCENOSIS IN HEALTHY PEOPLE



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Abstract

The skin microbiota contains non-diphtheria corynebacteria that can exhibit both pathogenic and beneficial properties. Genomic analysis of clinical isolates of Corynebacterium spp. will provide a more complete picture regarding their pathogenicity or positive effects. Objective: to characterize the role of some clinical isolates of Corynebacterium spp. in shaping the skin microbiocenosis of healthy people based on assessing their genome. Material and methods. Isolates of C. pseudodiphtheriticum ЩХ1, C. glutamicum ЩХ5, C. variable ЩХ7 from the skin surface of conditionally healthy individuals were identified using mass spectrometry. Whole genome sequencing of bacterial strains and genomic analysis were performed. The virulence of Corynebacterium spp. strains was determined using the Galleria mellonellа larval model. The strains were deposited in the State Collection of Pathogenic Microorganisms and Cell Cultures "GKMP-Obolensk", the genome sequences were placed in the GenBank database. Results. According to the phylogenetic analysis, the strains C. pseudodiphtheriticum ЩХ1, C. glutamicum ЩХ5 and C. variable ЩХ7 form separate clades, and C. variable ЩХ7 occupies a separate place, having a high phylogenetic proximity to mycobacteria. All isolates contain genes responsible for metabolism, stress protection, synthesis of amino acids, vitamins, and terpenes. Genes of the mntАBС system associated with resistance to hydrogen peroxide, superoxide radicals and the ability to use Mn2+ as an alternative cofactor in case of environmental iron deficiency were found only in C. pseudodiphtheriticum ЩХ1. The ent genes regulating siderophore formation were found in the strains C. glutamicum ЩХ5 and C. variable ЩХ7. The spectrum of AMP resistance genes is wider in C. pseudodiphtheriticum ЩХ1 and C. glutamicum ЩХ5, and the genes presumably associated with AMP resistance are more common in C. variable ЩХ7. Conclusion. C. pseudodiphtheriticum ЩХ1, C. glutamicum ЩХ5 and C. variable ЩХ7 contain genes associated with metabolism, stress resistance, and the ability to survive in the human body. They lack true pathogenicity genes in the presence of polyfunctional genes associated with both metabolism and pathogenicity. Corynebacterium spp. isolates may produce useful substances (amino acids, vitamins, terpenes). This indicates their positive role in shaping human skin microbiocenosis.

About the authors

Galina Kharseeva

Federal State Educational Institution of Higher Education «Rostov State Medical University» Ministry of Health of Russia, Rostov-on-Don, Russian Federation

Email: galinagh@bk.ru
ORCID iD: 0000-0002-6226-2183
SPIN-code: 7740-4921
Scopus Author ID: 35787921700

MD, Professor, Head of the Department of Microbiology and Virology 2

Russian Federation, Federal State Educational Institution of Higher Education «Rostov State Medical University» Ministry of Health of Russia, 344022, Rostov-on-Don, Russian Federation

Olga Sergeevna Shcherbataya

Federal State Educational Institution of Higher Education «Rostov State Medical University» Ministry of Health of Russia, Rostov-on-Don, Russian Federation

Email: olgo4ka86@rambler.ru
ORCID iD: 0000-0002-0507-3853
SPIN-code: 5314-7293

Senior Laboratory Assistant Head of the Department of Microbiology and Virology № 2 

Russian Federation, Rostov-on-Don, Nakhichevansky Lane, 29, 344022

Elvira Lvovna Alutina

Federal State Educational Institution of Higher Education «Rostov State Medical University» Ministry of Health of Russia, Rostov-on-Don, Russian Federation

Email: Alutina_el@rostgmu.ru
ORCID iD: 0000-0001-6968-0583
SPIN-code: 8657-9480
Scopus Author ID: 26657210900

Candidate of Medical Sciences, Associate Professor Head of the Department of Microbiology and Virology № 2

Russian Federation, Rostov-on-Don, Nakhichevansky Lane, 29, 344022

Oksana Andreevna Podoynitsina

Plague Control Research Institute of Rospotrebnadzor, Rostov-on-Don, Rostov-on-Don, Russian Federation

Email: podoynitcina_oa@antiplague.ru
ORCID iD: 0000-0002-9996-4189
SPIN-code: 4424-8963

Dr. Sci. (Biol.) senior researcher  at the Department of Microbiology of Cholera

Russian Federation, 117/40 M. Gorky Street, Rostov-on-Don, 344002.

Natalia Evgenievna Gaevskaya

Plague Control Research Institute of Rospotrebnadzor, Rostov-on-Don, Rostov-on-Don, Russian Federation

Email: gaevskaya_ne@antiplague.ru
ORCID iD: 0000-0002-0762-3628
SPIN-code: 3143-4788

Dr. Sci. (Med.), director 

Russian Federation, 117/40 M. Gorky Street, Rostov-on-Don, 344002

Anna Alexandrovna Alieva

Federal State Educational Institution of Higher Education «Rostov State Medical University» Ministry of Health of Russia, Rostov-on-Don, Russian Federation

Email: anna1976rita@mail.ru
ORCID iD: 0000-0002-3260-0209
SPIN-code: 6569-9880

candidate of biological sciences, assistant Head of the Department of Microbiology and Virology № 2 

Russian Federation, Rostov-on-Don, Nakhichevansky Lane, 29, 344022

Natalia Aleksandrovna Voronina

Federal State Educational Institution of Higher Education «Rostov State Medical University» Ministry of Health of Russia, Rostov-on-Don, Russian Federation

Email: vvv.natali@mail.ru
ORCID iD: 0000-0002-9655-6876
SPIN-code: 4891-1889
Scopus Author ID: 55327121500

candidate of biological sciences, assistant Head of the Department of Microbiology and Virology № 2 

Russian Federation, Rostov-on-Don, Nakhichevansky Lane, 29, 344022

Svetlana Yuryevna Tyukavkina

Federal State Educational Institution of Higher Education «Rostov State Medical University» Ministry of Health of Russia, Rostov-on-Don, Russian Federation

Email: svetlanava@mail.ru
ORCID iD: 0000-0002-4490-7013
SPIN-code: 2796-8796

Candidate of Medical Sciences, Associate Professor Head of the Department of Microbiology and Virology № 2 

Russian Federation, Rostov-on-Don, Nakhichevansky Lane, 29, 344022

Svetlana Yuryevna Temyakova

Plague Control Research Institute of Rospotrebnadzor, Rostov-on-Don, Rostov-on-Don, Russian Federation

Author for correspondence.
Email: temyakova_syu@antiplague.ru
ORCID iD: 0000-0003-4472-0264
SPIN-code: 2120-4169

researcher at the Laboratory of Molecular Biology of Natural Focal and Zoonotic Infections 

Russian Federation, 117/40 M. Gorky Street, Rostov-on-Don, 344002

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