2024-03-28T13:36:14Z
https://iimmun.ru/index/oai
oai:oai.iimmun.ru:article/1572
2020-08-07T07:04:12Z
iimm:SHORT
oai:iimmun.ru:article/176
2022-04-13T15:15:28Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/176
2022-04-13T15:15:28Z
Russian Journal of Infection and Immunity
Vol 3, No 3 (2013); 279-284
CLINICAL SIGNIFICANCE OF SERUM LEVELS OF CYTOKINES AND TOTAL IgE IN CHILDREN OF DIFFERENT AGE WITH SALMONELLOSIS
Zheleznikova G.F.; Institute of Children’s Infections, Saint Petersburg
Bechtereva M.K.; Institute of Children’s Infections, Saint Petersburg
Volohova O.A.; Institute of Children’s Infections, Saint Petersburg
Monahova N.E.; Institute of Children’s Infections, Saint Petersburg
2014-07-11 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/176
цитокины; общий IgE; сальмонеллез; дети
ru
Abstract. The serum levels of cytokines and total IgE in 113 children with salmonellosis were tested in ELISA. It was established, that severe course of infection in chidren up to 4 years of age is associated with low level of IL-10, and after 4 years of age with IFNγ deficit. Low level of total IgE is the marker of severe salmonellosis too, particularly in children up to 4 years of age.
oai:iimmun.ru:article/1268
2022-04-12T15:56:40Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/1268
2022-04-12T15:56:40Z
Russian Journal of Infection and Immunity
Vol 11, No 3 (2021); 565-569
Polymorphism of pro-inflammatory cytokine genes in acute intestinal infections
https://iimmun.ru/iimm/article/download/1268/6681
https://iimmun.ru/iimm/article/download/1268/6682
https://iimmun.ru/iimm/article/download/1268/6683
https://iimmun.ru/iimm/article/download/1268/6684
https://iimmun.ru/iimm/article/download/1268/6685
https://iimmun.ru/iimm/article/download/1268/6686
https://iimmun.ru/iimm/article/download/1268/6687
https://iimmun.ru/iimm/article/download/1268/6688
https://iimmun.ru/iimm/article/download/1268/6689
https://iimmun.ru/iimm/article/download/1268/6690
https://iimmun.ru/iimm/article/download/1268/10751
Epifantseva N.V.; Chita State Medical Academy
Vitkovsky Y.A.; Chita State Medical Academy
Emelyanova A.N.; Chita State Medical Academy
2021-05-13 12:28:12
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1268
острые кишечные инфекции; воспаление; полиморфизм; цитокины; генотип; IL-2 T330G; IL-1β Т31С
ru
Acute intestinal infections are widespread and hold the second place among infectious diseases, giving way solely to respiratory diseases. In this regard, much attention has been paid to examining acute intestinal infections, including immunopathogenetic mechanisms. And since proinflammatory and antiinflammatory cytokines play an important role in development of inflammatory reactions affecting disease severity and outcome, it becomes reasonable to study polymorphism of genes governing production of related molecules. Thus, the aim of our study was to examine the polymorphism in the IL-1β Т31С and IL-2 T330G genes; such mutations were characterized by nucleotide replacement affecting the gene promoters, which influenced production rate and level of the relevant cytokines. There were enrolled 108 patients with acute intestinal infections comprising main group as well as 94 apparently healthy subjects in the control group. Genomic DNA was isolated from the whole blood leukocytes by using a DNA-express-blood reagent, followed by conducting amplification reaction with two pairs of allele-specific primers. The polymorphism in the IL-1β, IL-2 genes was determined by PCR with primers purchased from Litech LLC (St. Petersburg). Data processing was carried out by using the statistical Statistica 6 suite software. While assessing the carriage rate of IL-1β T31C gene polymorphic markers by using the multiplicative inheritance model in both groups, the prevalence of the normal T allele and, respectively of the —31TT and —31TC genotypes with OR = 1.83 and an interval of 1.04—3.22 (χ2 = 6.35, p = 0.04, df = 2) was found, which allowed us to identify a relationship between the carriage of IL-1β в gene heterozygous variant and potentially elevated risk of AII. Regarding the IL-2 T330G gene, it was found that pathological G alleles was more markedly abundant in patients with acute intestinal infections compared to control group. Analyzing diverse IL-2 T330G carriage rate in patients with acute intestinal infections revealed that carriers of the TG heterozygous variant predominated — 56.48% (χ2 = 17.75, F = 0.000031), whereas pathological genotype GG was found in 13.89% (χ2 = 12.31, F = 0.000663, p < 0.05), with high probability of the relationship between carriage of these genotypes and a risk of disease development (OR — 3.63 [1.97—6.68] and OR — 6.91 [2.12—22.59]). Hence, the carriage of polymorphic variants of the IL-1β T31C and IL-2 T330G genes was associated with elevated risk of developing AII in case of infection with pathogenic microorganisms.
oai:iimmun.ru:article/2007
2023-01-06T21:08:09Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/2007
2023-01-06T21:08:09Z
Russian Journal of Infection and Immunity
Vol 12, No 6 (2022); 1163-1168
Immunochemical activity of <i>Yersinia pseudotuberculosis</i> ompF and ompC porins evaluated by optical trapping
https://iimmun.ru/iimm/article/download/2007/14035
https://iimmun.ru/iimm/article/download/2007/14036
https://iimmun.ru/iimm/article/download/2007/14037
https://iimmun.ru/iimm/article/download/2007/14038
https://iimmun.ru/iimm/article/download/2007/14039
https://iimmun.ru/iimm/article/download/2007/14040
https://iimmun.ru/iimm/article/download/2007/14041
https://iimmun.ru/iimm/article/download/2007/14044
https://iimmun.ru/iimm/article/download/2007/14051
https://iimmun.ru/iimm/article/download/2007/14052
https://iimmun.ru/iimm/article/download/2007/14053
https://iimmun.ru/iimm/article/download/2007/14054
https://iimmun.ru/iimm/article/download/2007/14055
https://iimmun.ru/iimm/article/download/2007/14821
https://iimmun.ru/iimm/article/download/2007/15337
Konyshev I.V.; Institute of Physiology of the Komi Scientific Center of the Ural Branch of the Russian Academy of Sciences; Russian Federation b Vyatka State University
Novikova O.D.; Pacific Institute of Bioorganic Chemistry named after G.B. Elyakov of the Far East Branch of the Russian Academy of Sciences
Portnyagina O.Y.; Pacific Institute of Bioorganic Chemistry named after G.B. Elyakov of the Far East Branch of the Russian Academy of Sciences
Byvalov A.A.; Institute of Physiology of the Komi Scientific Center of the Ural Branch of the Russian Academy of Sciences; Vyatka State University
2022-12-30 21:01:17
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/2007
порины; Yersinia pseudotuberculosis; антигены; антитела; сенсибилизация; лазерная ловушка
Array
FUUUU-2022-0065
ru
Introduction. Study of features for interacting “antigen-antibody” system is of great importance for developing new modern tools for diagnostics and therapy of infectious diseases. In this regard, it is of great interest to study the rupture force between bacterial antigens and antibodies using modern biophysical methods including optical trapping. The importance of surface antigens in the immunochemical activity of Yersinia pseudotuberculosis assessed by such method has not been evaluated yet. In this work we examined an opportunity to evaluate the interaction of hydrophobic Y. pseudotuberculosis porins OmpF and OmpC with specific antibodies using optical trapping method. Materials and methods. Polystyrene microspheres (d = 1 μm) were coated by passive adhesion with purified preparations of OmpF and OmpC porins; microsphere sensitization was verified by enzyme immunoassay. Antibodies from mouse sera were adsorbed onto the glass surface by chemical linking. The rupture force in the “porins-antibodies” system was determined using a laser trap according to the previously developed algorithm. Results. Using a model system including polystyrene microspheres sensitized with the proteins and aminated glass substrate coated with immune or nonimmune serum, significant differences in binding strength of OmpF and OmpC porins to homologous immune versus nonimmune sera were detected. The average forces of interaction with immune sera was 60 pN for OmpF microspheres (control — 40 pN) and 69 pN for OmpC microspheres (control — 49 pN). The proportion of irreversible substrate binding of the microspheres coated by the antigens to the treated with immune vs. non-immune sera was significantly higher. The results of assessing the average interaction force, as well as the predominance of the proportion of irreversible binding of antigen-coated microspheres with sera-treated substrates, indicates that specific interactions contribute significantly to the force of interaction. The aforementioned method can be used to evaluate the forces of intermolecular interaction in similar model systems using other microbial antigens.
oai:iimmun.ru:article/741
2022-04-12T15:22:28Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/741
2022-04-12T15:22:28Z
Russian Journal of Infection and Immunity
Vol 8, No 2 (2018); 219-222
BACTERIAL AND VIRAL PATHOGENS IN IXODES SP. TICKS IN ST. PETERSBURG AND LENINGRAD DISTRICT
Panferova Y.A.; St. Petersburg Pasteur Institute
Suvorova M.A.; Explana JSC, St. Petersburg
Shapar A.O.; Center of State Sanitary and Epidemiological Surveillance
Tokarevich N.K.; St. Petersburg Pasteur Institute
North-Western State Medical University named after I.I. Mechnikov
2018-09-10 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/741
клещевые патогены;вирус клещевого энцефалита;боррелии;риккетсии;инфицированность;ПЦР-диагностика
ru
Tick-borne infections are the most common group of zooanthroponotic diseases in the Northern Hemisphere. For the Baltic Sea region and Fennoscandia, the dominant infectious pathologies transmitted by ticks are tick-borne borreliosis and tick- borne encephalitis. The presence of vast forested areas, actively visited by people in St. Petersburg and the Leningrad region, contributes to a rather high level of encroachment on the flares and intelligence of the borreliosis and tick-borne encephalitis among the population of these regions. The relatively dangerous pathogens that can be transmitted with the tick bite are also of particular danger: Anaplasma sp., Ehrlichia sp., Coxiella burnetii, Rickettsia sp. In this work, detection was performed using molecular genetic methods of TBE virus, B. burgdorferi sensu lato and Rickettsia sp. in engorged ticksple, as well as questing ticks collected from vegetation. The established levels of infection of TBE on infected ticks, levels of infection by pathogenic Borrelia of questing and engorgeded ticks were approximately equal. Rickettsia was not found in the ticks. The conducted analysis of the pathogens prevalence in comparison with the data of russian and foreign authors. Monitoring the prevalence of tick-borne pathogens is an important issue in the prevention of tick- borne infections in the North-Western Russia.
oai:iimmun.ru:article/1352
2022-04-12T15:51:42Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/1352
2022-04-12T15:51:42Z
Russian Journal of Infection and Immunity
Vol 10, No 2 (2020); 368-374
The local interferon-corrective therapy in children with congenital cleft lip and palate, suffering from the recurrent respiratory infections
https://iimmun.ru/iimm/article/download/1352/7407
https://iimmun.ru/iimm/article/download/1352/7408
https://iimmun.ru/iimm/article/download/1352/7409
https://iimmun.ru/iimm/article/download/1352/7410
https://iimmun.ru/iimm/article/download/1352/7411
https://iimmun.ru/iimm/article/download/1352/7412
https://iimmun.ru/iimm/article/download/1352/7413
https://iimmun.ru/iimm/article/download/1352/7414
https://iimmun.ru/iimm/article/download/1352/7415
https://iimmun.ru/iimm/article/download/1352/7416
https://iimmun.ru/iimm/article/download/1352/7417
https://iimmun.ru/iimm/article/download/1352/7418
https://iimmun.ru/iimm/article/download/1352/8317
https://iimmun.ru/iimm/article/download/1352/8319
https://iimmun.ru/iimm/article/download/1352/8772
Nesterova I.V.; RUDN University of Ministry of Education and Science of Russia; Kuban State Medical University of Ministry of Health Development of Russia
Mitropanova M.N.; RUDN University of Ministry of Education and Science of Russia
Chudilova G.A.; RUDN University of Ministry of Education and Science of Russia
Kovaleva S.V.; RUDN University of Ministry of Education and Science of Russia
Khalturina E.O.; I.M. Sechenov First Moscow State Medical University (Sechenov University)
2020-05-18 15:07:06
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1352
врожденная расщелина губы и неба; рецидивирующие респираторные инфекции; иммунокомпрометированные дети; комплексная иммунореабилитация; иммунотропная терапия; мукозальный иммунитет
en
It is known that children with congenital cleft lip and palate are suffering from recurrent respiratory infections, which worsen the state of their health, and also complicate the results of reconstructive surgical treatment. The aim of the study was to detect defects of mucosal immunity in children with congenital cleft lip and palate, suffering from recurrent respiratory infections, and to create the program of local interferon corrective therapy with an assessment of its effectiveness. The studies included 56 children from the age of 1 to 3 years. Three groups of children were formed: group 1 – 26 children with congenital cleft lip and palate (antibiotic therapy); group 2 — 30 children with congenital cleft lip and palate (antibiotic therapy + local interferon therapy), group 3 — the control group. The clinical examination included a medical history, an assessment of the symptoms of recurrent episodes of acute respiratory infections and exacerbations of chronic infections. Microbiological studies were performed using standard methods. The status of local immunity was detected: the concentrations of secretory IgA, cytokines IL-17, IL-4, IL-6, IL-1β, IFNγ in the oral fluid were tested by ELISA. Results of the study established that in group 1 and group 2 clinical criteria of immunodeficiency with an infectious syndrome were revealed: repeated acute respiratory viral infections from 10 or more times a year, complicated by frequent exacerbations of chronic bacterial infection (up to 10 or more per year). Assessment of the state of local immunity in children with congenital cleft lip and palate revealed a lack of sIgA compared with the control group. Before treatment in group 2 oral fluid level of IL-17, IL-6 were statistically significant increase (p < 0.05); the results of the study also established increase in the level of IL-1β and a decrease in anti-inflammatory IL-4 and regulatory IFNγ relative to the control group (p > 0.05). After complex treatment with the inclusion of local interferon therapy in group 2 the appearance of sIgA, increase in the concentration of IL-4, IL-1β and a decrease IL-17 in oral fluid were observed (p > 0.05). The concentrations of IL-6, IFNγ did not change (p > 0.05). After treatment in group 2 there were a decrease in exacerbations of chronic upper respiratory tract infection and in frequency of acute respiratory viral infections compared with group 1 (p < 0.05). Positive clinical efficacy of local interferon therapy (the gel of recombinant IFNα2b in combination with oxidants — Viferon gel) in the process of staged rehabilitation of children with congenital cleft lip and palate has a protective clinical effect in reducing the frequency of acute respiratory viral infections, reducing the number of postoperative complications, reducing hospital stay, duration of antibacterial therapy and the number of exacerbations of chronic bacterial infection.
oai:iimmun.ru:article/84
2022-04-12T21:20:09Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/84
2022-04-12T21:20:09Z
Russian Journal of Infection and Immunity
Vol 2, No 3 (2012); 665-668
THE OUTBREAK OF PARVOVIRAL INFECTION IN THE TERRITORY OF NORTH-WESTERN FEDERAL REGION OF RUSSIA
Spiridonova L.A.; ФБУЗ «Центр гигиены и эпидемиологии в Ленинградской области», Ленинградская область
Antipova A.Y.; ФБУН НИИ эпидемиологии и микробиологии имени Пастера, Санкт-Петербург
Lavrentieva I.N.; ФБУН НИИ эпидемиологии и микробиологии имени Пастера, Санкт-Петербург
Bichurina M.A.; ФБУН НИИ эпидемиологии и микробиологии имени Пастера, Санкт-Петербург
Surkova V.V.; МБУЗ «Центральная больница Ломоносовского района», Ленинградская область
2014-07-02 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/84
экзантемная инфекция; вспышка; парвовирусная инфекция; лабораторная диагностика
ru
Abstract. The results of clinical, epidemiological and laboratory investigation of outbreak of exanthemic infection occurred in the Lomonosov district of Leningrad oblast in May, 2012 are presented in this article. The clinical diagnosis of parvoviral infection was laboratory confirmed by specific IgM antibodies detection in patients’ sera by ELISA. It is emphasized the importance of virological monitoring for viral infections on the stage of measles elimination and sporadic level of rubella cases.
oai:iimmun.ru:article/389
2022-04-12T15:18:13Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/389
2022-04-12T15:18:13Z
Russian Journal of Infection and Immunity
Vol 6, No 1 (2016); 87-92
LIVING MICROORGANISM’S STABILYZATION IN BIOMASS BIOTECHNOLOGY AND PLAGUE VACCINE PREPARATION
Budika D.A.; Stavropol Plague Control Research Institute, Stavropol, Russian Federation
Abzaeva N.V.; Stavropol Plague Control Research Institute, Stavropol, Russian Federation
Gostischeva S.E.; Stavropol Plague Control Research Institute, Stavropol, Russian Federation
Rakitina E.L.; Stavropol Plague Control Research Institute, Stavropol, Russian Federation
Ivanova G.F.; Stavropol Plague Control Research Institute, Stavropol, Russian Federation
Fisun A.A.; Stavropol Plague Control Research Institute, Stavropol, Russian Federation
2016-06-09 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/389
биотехнология;вакцина чумная живая;жизнеспособность;иммуногенность;термостабильность;моноклональные антитела
ru
Over the years, the production release of the plague vaccine is well developed its technology. The technological cycle of production of the preparation consists of regulated steps, however, despite their effectiveness it is necessary to modernize the manufactoring process, for example, solutions for some of the pressing needs of the customers, in particular, small groups of immunization. Our research has focused on obtaining experimental samples plague vaccine smaller compared to the commercial vaccine, the number of doses per vial prepared in a biomass production unit (ACM-Sh) surface by cultivation using all regulated processing steps, except step of combining content two swabs, and then an additional dilution of the cell suspension stabilizer. However, the time information and the subsequent preparation of such a vaccine is excluded us, since biomass is the second flush in quantitative terms is a ready raw material for the preparation of reduced dosage. The benefits of receiving the vaccine reduced the number of doses directly from the biomass of the second flush with the concentration of microbial cells Yersinia pestis EV 20–40 × 109 biotechnology greatly simplify the manufacture of such a preparation. The experimental vaccine series were tested by major regulated parameters: optical concentration, vitality, thermal stability, the loss on drying. In addition, the vaccine was prefabricated with high baseline viability to extreme temperatures (37±1)°C for 24 hours to exclude enough viable microbial cells for subsequent stabilization indicator of viability during storage. It should be noted that all the experimental samples preserved viability index not lower regulated (25%) during the experiment, in contrast to the commercial preparation. To determine the stability of the formulation during storage (over 3 years) was a comparative analysis of the viability of the experimental and commercial lots. To assess post vaccination immune analyzed the immune response to the introduction of a plague vaccine using FACSCalibur flow cytometer, considering that this technology has a high specificity, sensitivity and informativity. With regard to the immunogenic properties, the active component is recorded at a very high level as the white mice, and guinea pigs. Thus, the main biological indicators derived preparations (viability, thermal stability, storage stability) exceed those of commercial analog and provide effective immunological alterations and highly immunogenic in experimental animals.
oai:iimmun.ru:article/1552
2022-04-12T16:32:41Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/1552
2022-04-12T16:32:41Z
Russian Journal of Infection and Immunity
Vol 12, No 1 (2022); 185-192
Antimicrobial resistance monitoring of potential pathogens causing bloodstream infections
https://iimmun.ru/iimm/article/download/1552/9363
https://iimmun.ru/iimm/article/download/1552/9365
https://iimmun.ru/iimm/article/download/1552/9366
https://iimmun.ru/iimm/article/download/1552/9367
https://iimmun.ru/iimm/article/download/1552/9368
https://iimmun.ru/iimm/article/download/1552/9369
https://iimmun.ru/iimm/article/download/1552/9370
https://iimmun.ru/iimm/article/download/1552/9371
https://iimmun.ru/iimm/article/download/1552/9372
https://iimmun.ru/iimm/article/download/1552/9373
https://iimmun.ru/iimm/article/download/1552/9374
https://iimmun.ru/iimm/article/download/1552/9377
https://iimmun.ru/iimm/article/download/1552/9378
https://iimmun.ru/iimm/article/download/1552/9379
https://iimmun.ru/iimm/article/download/1552/9380
https://iimmun.ru/iimm/article/download/1552/9381
https://iimmun.ru/iimm/article/download/1552/9383
https://iimmun.ru/iimm/article/download/1552/12103
https://iimmun.ru/iimm/article/download/1552/12104
https://iimmun.ru/iimm/article/download/1552/12105
https://iimmun.ru/iimm/article/download/1552/12472
Zakhvatova A.S.; North-Western State Medical University named after I.I. Mechnikov; St. Petersburg Medical Information and Analytical Center
Daryina M.G.; North-Western State Medical University named after I.I. Mechnikov; St. Petersburg Medical Information and Analytical Center
Svetlichnaya Y.S.; North-Western State Medical University named after I.I. Mechnikov; St. Petersburg Medical Information and Analytical Center
Zueva L.P.; North-Western State Medical University named after I.I. Mechnikov
Aslanov B.I.; North-Western State Medical University named after I.I. Mechnikov
Chervyakova M.A.; North-Western State Medical University named after I.I. Mechnikov
2022-01-21 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1552
антимикробная резистентность микроорганизмов; возбудители инфекций кровотока; эпидемиологическая безопасность; инфекции; связанные с оказанием медицинской помощи; полирезистентность возбудителей; антимикробная терапия
ru
Microbiological monitoring, being an integral component of epidemiological surveillance of healthcare associated infection (HAI), is one of the most important components in hospital patient safety. The purpose of monitoring is the etiological interpretation of pathogens, the identification of microbial hospital strains and development of strategies as well as tactics for combating them. In modern conditions development of healthcare and humanity as a whole, the most important problem is the steady increase in the number of HAI caused by antimicrobial drug-insensitive pathogens and decreased drug effectiveness used in therapy. The structure of infectious conditions in the hospital is specific and is determined by the profile and nature of the treatment, nosology and age of patients. The main pathogens in the etiological structure of HAI are Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumanii, Enterococcus faecalis and Enterococcus faecium. The study estimated the prevalence of bloodstream infections pathogens resistant to antimicrobial agents in healthcare facilities in St. Petersburg. We retrospectively analyzed data on the antimicrobial resistance for the six clinically significant HAI pathogens that were detected in the blood samples at the 50 hospitals of St. Petersburg during the years 2016–2019. It was found that the use of β-lactam antibiotics is not effective in the treatment of patients with bloodstream infections (BSI) caused by S. aureus (in 21.9% of BSI cases), E. coli (2.1%), Klebsiella spp. (39.6%), P. aeruginosa (40.5%), Acinetobacter spp. (66.7%), Enterococcus spp. (4.4%). This article shows that targeted microbiological monitoring of HAI caused by resistant pathogens should be considered as an effective organizational mechanism for timely detection and prevention of complications that are observed in medical care to patients at high risk for bloodstream infections. Identification of the features of the epidemic process for purulentseptic infections in medical organizations contributes to the improved preventive measures in order to improve the quality of medical care. Microbiological monitoring data are necessary for the development and implementation of measures to curb the further growth of antimicrobial resistance of infectious disease pathogens.
oai:iimmun.ru:article/9635
2023-12-15T21:47:59Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/9635
2023-12-15T21:47:59Z
Russian Journal of Infection and Immunity
Vol 13, No 5 (2023); 967-971
Prognostic significance of interleukin-1a (il1a) RS1800857 genetic polymorphism in develop pulmonary sarcoidosis in residents of Karelia
https://iimmun.ru/iimm/article/download/9635/87152
https://iimmun.ru/iimm/article/download/9635/87160
https://iimmun.ru/iimm/article/download/9635/87161
https://iimmun.ru/iimm/article/download/9635/87162
https://iimmun.ru/iimm/article/download/9635/87163
https://iimmun.ru/iimm/article/download/9635/87164
https://iimmun.ru/iimm/article/download/9635/87165
https://iimmun.ru/iimm/article/download/9635/153548
Malysheva I.E.; Institute of Biology of the Karelian Research Centre of the Russian Academy of Sciences
Topchieva L.V.; Institute of Biology of the Karelian Research Centre of the Russian Academy of Sciences
Tikhonovich E.L.; V.A. Baranov Republican Hospital
2023-11-30 23:31:28
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/9635
воспаление; гранулёма; саркоидоз лёгких; цитокины; инфекционные агенты; полиморфизм генов; ген IL1A
Array
ru
The relevance of the research problem is justified by insufficient knowledge of the mechanisms for genetic regulation of inflammatory immune response during granuloma formation and arising inflammation in pulmonary sarcoidosis. It is believed that development of inflammatory process and formation of sarcoid granulomas occurs in subjects with genetically determined sensitivity to the effects of an unidentified etiological agent(s). The complexity of determining a causative factor(s) is accounted for by a variety of clinical forms and manifestations of the disease as well as a role for multifaceted immunological events in the pathogenesis of this disease. The process of inflammation, its intensity, may depend, among other issues, on host genetic background. Both enhanced and lowered production of pro-inflammatory factors can be observed in carriers of certain combinations of gene allelic variants. This, in turn, may determine human susceptibility to emergence of pulmonary sarcoidosis as well as alter clinical characteristics of the disease course and magnitude of developing immune inflammatory response. It should also be noted that the genetic background differs in various ethnic groups. Therefore, genetic background and environmental cues, ethnicity, may account for differed disease prevalence and phenotype. In this regard, it is relevant to search for allelic gene variations that could act as prognostic markers for development and progression of pulmonary sarcoidosis as well as characterize the features of its course. The data on the relationship between the carriage of allelic gene variations and susceptibility to pulmonary sarcoidosis as well as the contribution of IL1A rs1800857 polymorphic variant to development, progression, and therapy of the disease remain sparse and often contradictory. The current study assessed a risk of lung sarcoidosis in the subjects of Russian descent of the Republic of Karelia. According to the results of the studies, a significant association (p < 0.001) between the indicated IL1A gene allelic polymorphism and pulmonary sarcoidosis was established, with a risk of its development increasing by 3.47-fold (95% CI 2.41–5.01) in carriers of the T allele (p < 0.001). Thus, the single nucleotide polymorphism rs1800857 in the IL1A gene is associated with the risk of developing lung sarcoidosis in the subjects of Russian descent of the Republic of Karelia.
oai:iimmun.ru:article/1164
2022-04-12T15:48:24Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/1164
2022-04-12T15:48:24Z
Russian Journal of Infection and Immunity
Vol 9, No 3-4 (2019); 595-599
Assessing survival rate of HPV-positive and HPV-negative cervical cancer patients
https://iimmun.ru/iimm/article/download/1164/5591
https://iimmun.ru/iimm/article/download/1164/5592
https://iimmun.ru/iimm/article/download/1164/5593
https://iimmun.ru/iimm/article/download/1164/5594
https://iimmun.ru/iimm/article/download/1164/5595
https://iimmun.ru/iimm/article/download/1164/5596
https://iimmun.ru/iimm/article/download/1164/5597
Ibragimova M.K.; Cancer Research Institute “Tomsk National Research Medical Center of the Russian Academy of Sciences”;
National Research Тomsk State University
Tsyganov M.M.; Cancer Research Institute “Tomsk National Research Medical Center of the Russian Academy of Sciences”
Churuksaeva O.N.; Cancer Research Institute “Tomsk National Research Medical Center of the Russian Academy of Sciences”
Kolomiets L.A.; Cancer Research Institute “Tomsk National Research Medical Center of the Russian Academy of Sciences”;
Siberian State Medical University
Litviakov N.V.; Cancer Research Institute “Tomsk National Research Medical Center of the Russian Academy of Sciences”;
National Research Тomsk State University
2019-11-15 18:58:13
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1164
рак шейки матки; вирус папилломы человека; высокий канцерогенный риск; вирусная нагрузка; выживаемость; прогноз
ru
Currently, studies highlighting features of emergence, development, clinical score and prognosis for patients with HPV-negative cervical cancer are scarce. However, the data regarding high recurrence rate and mortality in patients with HPV-negative head and neck cancer are demonstrated. Here, cervical canal and exocervical scraping samples collected from 116 patients with primary cervical cancer, I–IV stages, aged 24 to 79 years, were examined with real-time PCR assay for assessing prevalence of 12 high oncogenic risk human papillomavirus (HPV) strains, genotyping and viral load. It was found that 84 (72.4%) and 32 cervical cancer patients (27.6%) were positive and negative, respectively, for high oncogenic risk (HR) HPV strains. Based on these data, patients were further subdivided into two groups: HPV-positive and HPVnegative patient group. Genotyping HPV-positive samples revealed that HPV genotype 16 was found in 67.8% of cases that agrees with data published worldwide. In addition, relapse-free and overall survival (HPV-positive and HPV-negative patients) rate were also evaluated in both groups. It was demonstrated that survival rate was significantly decreased in HPVnegative cervical cancer patients additionally characterized by less favorable prognosis. Moreover, length of relapse-free survival as well as overall survival for HPV-positive vs. HPV-negative patients was 102 vs. 68 months as well as 52 vs. 83 months, respectively. On the other hand, it was demonstrated that recurrence rate, clinical score and progression of cervical cancer directly depend on cancer spread observed at primary medical examination. Of note, the majority of primary cervical cancer relapses are diagnosed within the first 2 years after completing treatment. In addition, an increasing relapse rate has been documented in cervical cancer patients at advanced stages. Upon that, biological cancer behavior remains poorly predictable even in patients at similar disease stage. Therefore, it is essential that HPV as an important prognostic factor would be taken into account for choosing proper therapeutic strategy for treatment of patients with cervical cancer.
oai:iimmun.ru:article/1415
2022-04-12T15:57:10Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/1415
2022-04-12T15:57:10Z
Russian Journal of Infection and Immunity
Vol 11, No 3 (2021); 570-576
Metabolic activity of peripheral blood neutrophils in patients with <i>Opisthorchis felineus</i> invasion and skin syndrome
https://iimmun.ru/iimm/article/download/1415/8123
https://iimmun.ru/iimm/article/download/1415/8124
https://iimmun.ru/iimm/article/download/1415/8125
https://iimmun.ru/iimm/article/download/1415/8126
https://iimmun.ru/iimm/article/download/1415/8127
https://iimmun.ru/iimm/article/download/1415/8128
https://iimmun.ru/iimm/article/download/1415/8129
https://iimmun.ru/iimm/article/download/1415/8130
https://iimmun.ru/iimm/article/download/1415/8131
https://iimmun.ru/iimm/article/download/1415/8828
https://iimmun.ru/iimm/article/download/1415/8829
https://iimmun.ru/iimm/article/download/1415/8830
https://iimmun.ru/iimm/article/download/1415/10748
Tsukanov V.V.; Research Institute of Medical Problems of the North, Federal Research Center KSC SB RAS
Gorchilova E.G.; Private Healthcare Institution Clinical hospital “RZD-Medicine” of Krasnoyarsk City
Kolenchukova O.A.; Research Institute of Medical Problems of the North, Federal Research Center KSC SB RAS
Gvozdev I.I.; Research Institute of Medical Problems of the North, Federal Research Center KSC SB RAS
Savchenko A.A.; Research Institute of Medical Problems of the North, Federal Research Center KSC SB RAS
Vasyutin A.V.; Research Institute of Medical Problems of the North, Federal Research Center KSC SB RAS
Tonkikh J.L.; Research Institute of Medical Problems of the North, Federal Research Center KSC SB RAS
Rzhavicheva O.S.; Private Healthcare Institution Clinical hospital “RZD-Medicine” of Krasnoyarsk City
Borisov A.G.; Research Institute of Medical Problems of the North, Federal Research Center KSC SB RAS
2020-08-23 17:35:28
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1415
Opisthorchis felineus; кожный синдром; нейтрофильные гранулоциты; функциональная активность; фиброз печени; активные формы кислорода; хемилюминесценция
Krasnoyarsk Regional Fund of Science
ru
The aim was to examine functional activity of peripheral blood neutrophilic granulocytes in patients with Opisthorchis felineus invasion and skin syndrome. Materials and methods. A total of 92 patients with chronic opisthorchiasis were examined, of which 38 patients (20 males and 18 females, average age 35.7±3.9 years) and 54 patients (28 males and 26 females, average age 36.5±4.1 years) with/without skin syndrome, as well as 32 apparently healthy subjects (17 males and 15 females, average age 41.5 years). Detection of adult parasite eggs or bodies in the duodenal contents and/or feces was used as the major diagnostic method of opisthorchiasis used in all 92 opisthorchiasis patients. Liver fibrosis was assessed by elastometry method according to the METAVIR scale in all 92 opisthorchiasis patients. Functional activity of peripheral blood neutrophilic granulocytes was examined in all 92 opisthorchiasis patients and 32 healthy individuals from the control group by chemiluminescent analysis measuring intensity of reactive oxygen species production in spontaneous and zymosan-induced reactions in lucigenin and luminol-dependent processes. Results. Opisthorchiasis patients with vs. without skin syndrome suffered at higher rate from pain in the right hypochondrium, dyspepsia, astheno-vegetative syndrome, skin itching, blood eosinophilia, hyperbilirubinemia, cholestatic syndrome, cholecystitis, hepatomegaly, and had elevated serum immunoglobulin E level. Liver fibrosis F3—F4 according to METAVIR was determined in 18.4% and 11.1% of opisthorchiasis patients with vs. without skin syndrome (OR = 1.78; CI 0.57—5.57; p = 0.05). Both luminol- and lucigenin-dependent processes in opisthorchiasis patients with vs. without skin syndrome revealed significantly decreased functional activity of neutrophilic granulocytes which was evidenced by significantly decreased maximum intensity of reactive oxygen species production and the area under the chemiluminescence curve both in spontaneous and zymosan-induced reaction. Conclusion. The results suggest that the presence of skin syndrome in patients with opisthorchiasis is accompanied by more prominent clinical and laboratory manifestations, decreased functional activity of peripheral blood neutrophils, thereby allowing to consider it as a marker of intense pathology.
oai:iimmun.ru:article/1374
2022-04-12T15:58:09Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/1374
2022-04-12T15:58:09Z
Russian Journal of Infection and Immunity
Vol 11, No 4 (2021); 777-783
Enhancing the specific T cell immune response against micro- and nanoparticle immobilized antigen
https://iimmun.ru/iimm/article/download/1374/7659
https://iimmun.ru/iimm/article/download/1374/7660
https://iimmun.ru/iimm/article/download/1374/7661
https://iimmun.ru/iimm/article/download/1374/7662
https://iimmun.ru/iimm/article/download/1374/7663
https://iimmun.ru/iimm/article/download/1374/7664
https://iimmun.ru/iimm/article/download/1374/7665
https://iimmun.ru/iimm/article/download/1374/7666
https://iimmun.ru/iimm/article/download/1374/7667
https://iimmun.ru/iimm/article/download/1374/9048
https://iimmun.ru/iimm/article/download/1374/10913
Sakhabeev R.G.; Institute of Experimental Medicine
Polyakov D.S.; Institute of Experimental Medicine
Goshina A.D.; St. Petersburg State University
Vishnya A.A.; Herzen State Pedagogical University of Russia
Kudryavtsev I.V.; Institute of Experimental Medicine
Sinitcina E.S.; Institute of Chemistry, St. Petersburg State University; Institute of Macromolecular Compounds of the Russian Academy of Sciences
Korzhikov-Vlakh V.А.; Institute of Chemistry, St. Petersburg State University
Tennikova T.B.; St. Petersburg State University; Institute of Chemistry, St. Petersburg State University
Shavlovsky M.M.; Institute of Experimental Medicine; Pavlov First St. Petersburg State Medical University
2020-10-23 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1374
микрочастицы из гомополимера полимолочной кислоты; наночастицы из сополимера полимолочной
кислоты и полиэтиленгликоля; «ловушки» вирусов; зеленый флуоресцентный белок; клеточный иммунный ответ
The study was funded by Russian Science Foundation Grant No. 19-73-10045.
ru
The current study was a part of the project on generating viral particle traps occurring due to covalent immobilization on the interface of recombinant virus-specific polymer-based nano- and microparticles. It is assumed that protein-particle conjugates could be able to bind virions followed by engulfment by immune cells. The study was aimed to examine the effect of polylactic acid (PLA) and PLA block-copolymer with polyethylene glycol (PLA-PEG)-based micro- and nanoparticles on the cellular immune response against polymeric particle-bound antigen. Materials and methods. A recombinant chimeric protein beta-2-microglobulin — green fluorescent protein (β2M-sfGFP) was obtained by affine chromatography. The recombinant protein was immobilized onto the polymer particles, which were further used for mice immunization. Female F1 hybrid mice (CBA x C57BL) in experimental and control groups consisted of 4–6-month-old 15 animals (weighted 20–25 g). Intracellular cytokine staining was used to evaluate the cellular immune response. Results and discussion. It was shown that the nanoparticles of PLA block-copolymer with polyethylene glycol (PLA-PEG) were able to bind 10 microgram protein per 1 mg polymer. The polylactic acid nanoparticles were able to bind 2,3 microgram protein per 1 mg polymer. In experiment, mice in group 1 were immunized with 100 nm PLA-PEG particle-β2M-sfGFP conjugate, in group 2 — with same particles together with soluble β2M-sfGFP. In group 3, mice were immunized with 1400 nm PLA particles-β2M-sfGFP conjugate, and in group 4 — with same particles together with soluble protein. The spleens isolated 2 weeks after the four-time intraperitoneal immunization. Comparison of immune response between groups was assessed by nonparametric Kruskal–Wallis criterion with Tukey correction. It was shown that the number of antigen-specific CD4+ T cells produced to model protein was significantly higher after immunization with particle-β2M-sfGFP conjugate, as compared to control groups, wherein immunization was performed with a mixture of protein and unmodified particles (p < 0.001). It was found that the number of antigen-specific CD8+ T cells formed against β2m-sfGFP did not differ between all groups examined.
oai:iimmun.ru:article/1893
2023-01-19T14:31:54Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/1893
2023-01-19T14:31:54Z
Russian Journal of Infection and Immunity
Vol 12, No 5 (2022); 963-970
Cytokine profile in <i>in vitro</i> mouse macrophage culture infected with <i>Bacillus anthracis</i> spores with varying plasmid composition
https://iimmun.ru/iimm/article/download/1893/12795
https://iimmun.ru/iimm/article/download/1893/12796
https://iimmun.ru/iimm/article/download/1893/12797
https://iimmun.ru/iimm/article/download/1893/12798
https://iimmun.ru/iimm/article/download/1893/12799
https://iimmun.ru/iimm/article/download/1893/12800
https://iimmun.ru/iimm/article/download/1893/12801
https://iimmun.ru/iimm/article/download/1893/12802
https://iimmun.ru/iimm/article/download/1893/12803
https://iimmun.ru/iimm/article/download/1893/12804
https://iimmun.ru/iimm/article/download/1893/12805
https://iimmun.ru/iimm/article/download/1893/13709
https://iimmun.ru/iimm/article/download/1893/14046
https://iimmun.ru/iimm/article/download/1893/14874
Koteneva E.A.; Stavropol Plague Control Research Institute of Rospotrebnadzor; North Caucasus Federal University
Tsygankova O.I.; Stavropol Plague Control Research Institute of Rospotrebnadzor
Shcherbakova V.Y.; Stavropol Plague Control Research Institute of Rospotrebnadzor
Kalinin A.V.; Stavropol Plague Control Research Institute of Rospotrebnadzor
Rodionov I.S.; Stavropol Plague Control Research Institute of Rospotrebnadzor
Serdyukov V.V.; Stavropol Plague Control Research Institute of Rospotrebnadzor
Abramovich A.V.; Stavropol Plague Control Research Institute of Rospotrebnadzor
Kulichenko A.N.; Stavropol Plague Control Research Institute of Rospotrebnadzor
2022-11-16 17:27:59
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1893
сибирская язва; цитокины; макрофаги; плазмиды вирулентности; капсула; токсин; моделирование инфекции in vitro
Array
ru
Bacillus anthracis, the causative agent of anthrax, is able to exist both in environmental conditions (soil) and in the macroorganism. The manifestation of pathogenic properties of B. anthracis strains is determined by relevant plasmid composition, because the main toxin and the capsule-related virulence factors are located in bacterial plasmid. Modeling anthrax infection in vitro in macrophage culture might reveal an influence of individual B. anthracis strain characteristics on infection and development of infectious process. The aim of this study was to analyze cytokine level during infection of in vitro macrophage cell cultures with spores of anthrax microbe strains bearing varying plasmid composition. The dependence of the macrophage cell cytokine profile on the plasmid composition of B. anthracis strains was revealed while modeling anthrax infection in vitro. The presence of the toxin-producing plasmid pXO1 in anthrax microbe strains has a powerful stimulating effect on the production of macrophages J774A cell line cytokines. B. anthracis strains lacking the pXO1 plasmid virtually stimulated no production of IL-1β, caused very low secretion of IL-1α, IL-6, MCP-1, MIP-1α, MIP-1β, IL-12 (p70) and active G-CSF products. The low cytokine response of macrophage cells infected with monoplasmid strains bearing only the capsule-forming plasmid was due not only to the absence of a binary toxin, but also to disturbed regulation of capsule production associated with the absence of the atxA gene. The capsule, along with lethal and edematous toxins, belongs to the main virulence factors of B. anthracis, but strains lacking the pXO1 virulence plasmid, had its production impaired, because the main regulator of capsule synthesis is the atxA gene localized on the pXO1 plasmid being positively regulated by the acpA and acpB genes, so that strains lacking the toxin-forming plasmid, even in the presence of the encapsulation plasmid, elicit a weak cytokine response in infected cells. Diplasmid strains of B. anthracis, due to produced main virulence factors — a two-component toxin and a capsule, enforce macrophages (in the experiment) to actively produce IL-1β, IL-6, MCP-1, G-CSF, MIP-1α; MIP-1β, IL-12 (p70). Strains with moderate virulence and capable of capsulation in air virtually did not differ from highly virulent strains in terms of their effect on in vitro macrophage culture.
oai:iimmun.ru:article/599
2022-04-12T15:21:12Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/599
2022-04-12T15:21:12Z
Russian Journal of Infection and Immunity
Vol 7, No 4 (2017); 409-412
EPIDEMIOLOGY ASPECTS OF HOSPITAL-ACQUIRED INFECTION MORBIDITY IN HEALTH SURGERY HOSPITALS IN PRIMORSKY REGION
Martynova A.V.; Far Eastern Federal University, Vladivostok;
Pacific State Medical University, Vladivostok
Gorkavaya A.Y.; Far Eastern Federal University, Vladivostok
Abashina V.L.; Center of Hygiene and Epidemiology, Vladivostok
Semeikina L.M.; Center of Hygiene and Epidemiology, Vladivostok
2018-01-20 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/599
госпитальные инфекции; постинъекционные осложнения; инфекции мочевыводящего тракта; гнойно-септические инфекции; внутрибольничные инфекции; эпидемиология внутрибольничных инфекций
ru
The problem of prophylaxis and treatment of the hospital-acquired infections caused remains actual and urgent and stubborn in modern medicine. Appearance of antimicrobial agents resistant and high virulent strains adapting also to desinfectants. Development of innovative hi-tech diagnostic methods and treatment at wide circulation of microorganisms with multiple antimicrobial agents resistance defines need of continuous improvement of epidemiological surveillance and microbiological monitoring of this group of infections. Aim of project: to carry out the epidemiological analysis of a case rate of hospital-acquired infections in Primorsky Krai and to determine the specific role of hospital-acquired infections which arose in surgical hospitals and units. Material and methods: In work there were used statistical forms, e.g. No. 2 «The information on infectious and parasitic diseases» in Primorsky Krai. A research method — the retrospective epidemiological analysis. Results of a research: In 2015 in Primorsky Krai there were registered 159 cases of hospitalacquired infections, the indicator of a case rate made 0.05 per 1000 treated patients. In comparison with 2014 the case rate decreased, rate of depression made — 28%. According to the statistical reporting the greatest number of cases of hospital-acquired infections was registered in obstetrical institutions — 60 cases (38% of all hospital-acquired infections), in children’s hospitals — 46 (29%) and in surgical hospitals and units — 33 (21%). Other hospital-acquired infections are registered in out-patient and polyclinic institutions and in institutions of a therapeutic profile. In structure of hospitalacquired infections in the territory of Primorsky Krai in 2015, as well as in previous years, acute intestinal infections dominated (35 cases — 22%) and it is purulent — septic infections at newborns (34 cases — 21%). Conclusions: In the territory of Primorsky Krai the hospital-acquired infections are often registered in obstetrical institutions, in children’s and surgical hospitals and units. The tendency to hospital-acquired infections morbidity growthin surgical hospitals and units, most likely, not owing to augmentation of cases of postoperative complications, and at the expense of other nosologies.
oai:iimmun.ru:article/1333
2023-08-06T17:10:51Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/1333
2023-08-06T17:10:51Z
Russian Journal of Infection and Immunity
Vol 10, No 3 (2020); 570-574
Detection of measles cases in the Republic of Guinea in 2017–2018
Lavrentieva I.N.; Saint Petersburg Pasteur Institute
Bichurina M.A.; Saint Petersburg Pasteur Institute
Antipova A.Y.; Saint Petersburg Pasteur Institute
Camara J.; Université Gamal Abdel Nasser, Conakry, Guinea
Magassouba N.; Université Gamal Abdel Nasser, Conakry, Guinea
2020-02-27 21:06:21
Authors who publish with this journal agree to the following terms:
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Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1333
корь; программа элиминации кори; Африка; вакцинация; ревакцинация
en
In 2017, WHO reported 596 confirmed measles cases in Guinea Republic connected to the 2016–2017 epidemic outbreak that was stopped after additional immunization (SIA) against measles in two provinces of the country. Improving the effectiveness of SIA is associated with the identification of epidemiologically significant groups of the population. The aim of the study was to analyze 2017–2018 measles cases and assess population immunity to measles virus in the Republic of Guinea. Materials and methods. A total of 810 blood serum samples collected from patients with maculo-papular rash and clinical diagnosis “measles?” were tested for measles virus-specific IgM-and IgG antibody level. 445 sera of conditionally healthy individuals aged 7 months to 67 years were examined for anti-measles virus IgG antibody level. Immunoglobulins of classes M and G were detected by ELISA with test systems «Anti-Measles Virus ELISA (IgM)» (Euro immun, Germany) and «Anti-Measles Virus ELISA (IgG)» (Euroimmun, Germany). Results and discussion.In 2017–2018, the epidemic process of the measles in the Republic of Guinea proceeded very intensively, being markedly prevalent in children among age groups. In 2018, more than half of the cases (61.6%) were identified in children aged 1 to 5 years old; the second most abundant age group was children under one year (18.6%), probably due to violated measles vaccination, which in GR are subject to children of nine months of age. It was found that 16.4% of patients (60 out of 366) had documented data on measles vaccination. Potentially, high proportion of measles cases among pre-vaccinated subjects was due to insufficient immune response to a single immunization in children of 9 months of age. Moreover, lowered vaccine-related properties might also be violated “cold chain” during vaccine transportation occurring in tropical climate. Analyzing 445 subjects revealed that total number of measles virus seronegative subjects was 8.3%. However, the vast majority of them were children and young adults aged 7 months to 22 years, where 52.4% of seronegative subjects were identified. Thus, the data obtained indicate that intensive measles virus circulation in human population was continued that necessitate interventions for improving epidemiological surveillance, extend routine measles vaccination coverage and conduct SIAs against measles in GR.
oai:iimmun.ru:article/60
2022-04-12T21:19:52Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/60
2022-04-12T21:19:52Z
Russian Journal of Infection and Immunity
Vol 1, No 3 (2011); 279-283
THE PREVALENCE OF CHLAMYDIA TRACHOMATIS INFECTION IN SAINT-PETERSBURG
Kulyashova L.B.; ФГУН «Санкт-Петербургский научно-исследовательский институт эпидемиологии и микробиологии имени Пастера» Роспотребнадзора, Санкт-Петербург
Berezina L.A.; ФГУН «Санкт-Петербургский научно-исследовательский институт эпидемиологии и микробиологии имени Пастера» Роспотребнадзора, Санкт-Петербург
Zakrevskaya A.V.; ФГУН «Санкт-Петербургский научно-исследовательский институт эпидемиологии и микробиологии имени Пастера» Роспотребнадзора, Санкт-Петербург
Zhebrun A.B.; ФГУН «Санкт-Петербургский научно-исследовательский институт эпидемиологии и микробиологии имени Пастера» Роспотребнадзора, Санкт-Петербург
Mayatskaya M.V.; СПб ГУЗ «Городская станция переливания крови»
Makovskaya O.A.; ГОУ ВПО «Санкт-Петербургская государственная педиатрическая медицинская академия» Минздравсоцразвития России
2014-06-30 00:00:00
Authors who publish with this journal agree to the following terms:
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Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/60
превалентность; инфекция, ассоциированная с Chlamidia trachomatis; эффективность лабораторных тестов
ru
Abstract. Chlamydia trachomatis causes various diseases of reproductive organs. Only limited data on the incidence of urogenital chlamydia infection in the Russian Federation are available. The main goal of this study was to detect the prevalence of infection associated with Chlamydia trachomatis in citizens of St.Petersburg. Overall 3833 individuals including 2190 children and adolescents aged from 0 to 18 years old and 1643 adults aged 19–70 years old were tested in 2008–2010. This group included patients with acute and chronic pelvic inflammatory disease, infertility as well as patients without clinical symptoms of Chlamydia infection. The high level of Chlamydia trachomatis infection among newborns and dramatic increase of the infection among adolescents were determined. These results support urgent needs to develop adequate preventive measures to control Chlamydia trachomatis infection in the population. The effectiveness of different laboratory tests to detect Chlamydia trachomatis infection depends on the causative agent localization in the macroorganism, on acuity of infection process and on immune response. The combined using of different laboratory tests give the possibility to obtain objective results about Chlamydia etiology role in inflammatory disease of urogenital tract as well as about prevalence of infection in regions of the country.
oai:iimmun.ru:article/332
2023-08-06T17:27:11Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/332
2023-08-06T17:27:11Z
Russian Journal of Infection and Immunity
Vol 5, No 3 (2015); 273-278
THE IMMUNOCOMPETENT CELLS RECEPTORS RESEARCH UNDER EXPERIMENTAL INFLUENZA INFECTION IN VITRO
Lisakov A.N.; Research Institute Vaccine and Serum named after I.I. Mechnikov, Moscow, Russian Federation
Nagieva F.G.; Research Institute Vaccine and Serum named after I.I. Mechnikov, Moscow, Russian Federation
Barkova E.P.; Research Institute Vaccine and Serum named after I.I. Mechnikov, Moscow, Russian Federation
Gaiderova L.A.; Research Center for Expertise of Means Medical Application, Moscow, Russian Federation
Fedotov A.Y.; Research Institute Vaccine and Serum named after I.I. Mechnikov, Moscow, Russian Federation
Nikulina V.G.; Research Institute Vaccine and Serum named after I.I. Mechnikov, Moscow, Russian Federation
Gavrilova M.V.; Research Institute Vaccine and Serum named after I.I. Mechnikov, Moscow, Russian Federation
2015-11-30 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/332
мононуклеарные клетки;периферическая кровь;активация лимфоцитов;культивирование in vitro;вирусы гриппа;интерфероновые рецепторы;экспрессия рецепторов;проточная цитометрия
ru
Introduction. It is known that interferon is a cytokine and is a substantial part of the immune system necessary for antigenic challenge immune response full expression. Also it is considered that every antigen is an interferon inducer. Interferon induces antivirus response via binding to specific receptors, this receptors can be revealed straight on cell membranes of immune cells. Research objective. To evaluate the interferon inducer ability of some Influenza A virus strains upon indications of receptors functional activity (capacity) to alpha and gamma interferons on peripheral mononuclear blood cells (PBMC) induced in vitro by different Influenza A virus strains. Material and methods. The method is based on lymphocytes separation from the venous heparinized blood, with followed by in vitro lymphocytes inducing at temperature 36.5°С in the presence of 5% CO2. Blood samples were taken in different time intervals, labelled by mouse anti-idiotipyc FITCconjugated antibodies, structurally simulated human alpha and gamma interferon, samples were fixed with paraformaldehyde. Interferon receptors expression were performed by flow cytometer. Results. The in vitro experiments have determined the interferon-inducing ability of three influenza virus strains: A/PR8/34 (H1N1), A/Krasnodar/101/59 (H2N2) and A/ Ryazan/6103/87 (H3N2). MPBC blood sample (blood group was 0, Rh factor – positive) was induced by irradiated noninfectious allantoic fluid with hemagglutinating activity. Expression of alpha and gamma interferon receptors (alpha and gamma IFNR) on MPBC was determined by IFNR markers labelled with FITC and it (expression) was estimated by flow cytometer. In parallel we compared expression of alpha and gamma IFNR on MPBC in primed and non primed cells by low doses of human alpha interferon. It was found that expression of alpha and gamma IFNR on MPBC, induced influenza A/ PR8/34 (H1N1) antigen, with high hemagglutinating activity was higher in primed MPBC in comparison with non primed and higher then expression was induced by influenza virus A/Krasnodar/101/59 (H2N2) and A/Ryazan/6103/87 (H3N2) with lower hemagglutinating activity. It should be noted that IFN alpha receptor (IFNAR) expression on induced by influenza virus strain A/PR8/34 (H1N1) and primed by low doses of alpha interferon, repose on high level from induction point (1 hour) and protract during high level during 5 hours. Evaluation of gamma IFNR (IFNGR) expression level on MPBC induced by different influenza virus strains testify that firstly up-regulation IFNGR expression on MPBC primed by low doses of alpha interferon is absent and secondly up-regulation IFNGR on MPBC bear no relation with hemagglutinating activity of Influenza virus antigen. Conclusion. Experiment results clearly suggest that all influenza strains used carry the interferon induced ability which is possible to see by expression of IFNAR and IFNGR on MPBC induced by described above virus antigens. Rate of interferon induce ability in different influenza virus A connected on one side with virus hemagglutinating activity level in estimating IFNAR and on the other side with virus strains in estimating IFNGR.
oai:iimmun.ru:article/1652
2023-11-16T17:06:58Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/1652
2023-11-16T17:06:58Z
Russian Journal of Infection and Immunity
Vol 12, No 1 (2022); 179-184
Human cytopenia variants at diverse HIV infection stages
https://iimmun.ru/iimm/article/download/1652/10104
https://iimmun.ru/iimm/article/download/1652/10105
https://iimmun.ru/iimm/article/download/1652/10106
https://iimmun.ru/iimm/article/download/1652/10107
https://iimmun.ru/iimm/article/download/1652/10303
https://iimmun.ru/iimm/article/download/1652/10304
https://iimmun.ru/iimm/article/download/1652/10305
https://iimmun.ru/iimm/article/download/1652/12085
Baryshnikova D.V.; Omsk State Medical University
Mordyk A.V.; Omsk State Medical University
Puzyreva L.V.; Omsk State Medical University; Dalmatov D.M. Infectious Diseases Clinical Hospital No. 1
2021-11-22 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1652
ВИЧ-инфекция; цитопения; анемия; тромбоцитопения; лейкопения; иммунодефицит
ru
Over decades, HIV infection and its complications have been one of the most debated problems in the world. The human immunodeficiency virus not only weaken the immune system, but also disrupts normal hematopoiesis manifested as cytopenia (anemia, thrombocytopenia and neutropenia). Materials and methods. A retrospective analysis of cases of combined HIV infection and inhibited hematopoiesis was carried out according to hemogram data of patients admitted for treatment at the Infectious Clinical Hospital No. 1 named after D. Dalmatov, Omsk. The inclusion criteria were cytopenia during hospitalization detected in detailed blood test (by calculating hemoglobin level, counts of erythrocytes, leukocytes, platelets). The age of the patients included in the study differed: from 20 to 29 years — 27 patients (24.6%), from 30 to 39 years — 69 patients (62.7%), from 40 to 49 years — 13 patients (11.8%), over 50 years old 1 patient (0.9%). All patients had suppression of at least one hematopoietic cell lineage. Anemia was considered as decreased hemoglobin level below than 130 g/l in men and 120 g/l in women. Erythrocytopenia was considered as decreased erythrocyte count below 4.76 × 1012/L. Leukopenia was defined as decreased total count of leukocytes below 4.0 × 109/L, while a decrease in the absolute count of neutrophils below 1000 cells/μL was considered as neutropenia. Thrombocytopenia was determined as decreased platelet count below 150 × 109/L. Results. All patients had suppression of at least one hematopoietic cell lineage. 6 patients with stage 2 had one-cell lineage cytopenias, 7 — two-cell lineages. While analyzing the data obtained, it can be concluded that in patients with stage 2 HIV, inhibition of erythroid and platelet cell lineage predominates, whereas thrombocytopenia reached grade IV. At stage 3 HIV, all 7 patients had inhibition of only one cell lineage. In this group, the inhibition of hematopoiesis had a lighter degree in all hematopoietic cell lineages. In 46 patients with stage 4, there were various oppression of one of the hematopoietic cell lineages, in 44 patients there were two-cell lineage cytopenias. For patients with a more advanced stage of HIV, a decrease in the number of all cellular elements of the blood in the hemogram is characteristic; these disorders are more severe and persistent.
oai:iimmun.ru:article/2116
2023-08-06T17:51:49Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/2116
2023-08-06T17:51:49Z
Russian Journal of Infection and Immunity
Vol 13, No 2 (2023); 376-382
Optimization of storage conditions for Puumala virus based vaccine
https://iimmun.ru/iimm/article/download/2116/15352
https://iimmun.ru/iimm/article/download/2116/15353
https://iimmun.ru/iimm/article/download/2116/15354
https://iimmun.ru/iimm/article/download/2116/15355
https://iimmun.ru/iimm/article/download/2116/15356
https://iimmun.ru/iimm/article/download/2116/15357
https://iimmun.ru/iimm/article/download/2116/15358
https://iimmun.ru/iimm/article/download/2116/15359
https://iimmun.ru/iimm/article/download/2116/15360
https://iimmun.ru/iimm/article/download/2116/15361
https://iimmun.ru/iimm/article/download/2116/15362
https://iimmun.ru/iimm/article/download/2116/15363
https://iimmun.ru/iimm/article/download/2116/18594
https://iimmun.ru/iimm/article/download/2116/18595
https://iimmun.ru/iimm/article/download/2116/77582
https://iimmun.ru/iimm/article/download/2116/77583
https://iimmun.ru/iimm/article/download/2116/109531
https://iimmun.ru/iimm/article/download/2116/109535
https://iimmun.ru/iimm/article/download/2116/109542
Vetrova A.N.; Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of the Russian Academy of Sciences; Sechenov First Moscow State Medical University (Sechenov University)
Kurashova S.S.; Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of the Russian Academy of Sciences
Teodorovich R.D.; Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of the Russian Academy of Sciences
Popova Y.V.; Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of the Russian Academy of Sciences
Blinova E.A.; Central Research Institute of Epidemiology of The Federal Service on Customers’ Rights Protection and Human Well-being Surveillance
Nabatnikov P.A.; Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of the Russian Academy of Sciences
Tkachenko E.А.; Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of the Russian Academy of Sciences
Dzagurova T.K.; Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of the Russian Academy of Sciences
2023-04-24 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/2116
геморрагическая лихорадка с почечным синдромом; хантавирус Пуумала; хантавирусный вакцинный препарат; нейтрализующие антитела; иммунный ответ; человеческий сывороточный альбумин
Array
ru
We report the effects of storage conditions and human serum albumin addition to the vaccine composition on the immunogenic properties of an experimental vaccine against hemorrhagic fever with renal syndrome. To obtain an experimental vaccine, the Puumala virus, propagated in Vero cells culture, was concentrated, inactivated with beta-propiolactone, and purified by chromatography on the Capto™Core 700 multimodal sorbent (GE Healthcare). The target component in the vaccine was 2±0.2 × 106 of viral RNA copies/ml. The humoral immune response to the vaccine was determined by measuring the neutralizing antibodies in the blood serum of immunized Syrian hamsters (Mesocricetus auratus). It was revealed that the Puumala virus inactivated with beta-propiolactone induces a pronounced humoral immune response, which indicates preservation of relevant immunogenic epitopes. Аccording to our study, viral RNA is more stable during storage of the inactivated vs. intact virus. It was confirmed that full virus inactivation occurred after 24 hours of storage at 37°C. A significant decrease in the virus immunogenicity during the thermal inactivation was noted, which confirms the need for careful selection of storage conditions for thermolabile Hantaviruses. A direct correlation was observed between viral titer and viral RNA copy number/ml. Adding human serum albumin stabilized viral infectivity during storage: Puumala virus titer remained at the same level for 3 months of storage at 6±2°C, whereas without albumin, the titer decreased to undetectable level. The addition of 0.1% human serum albumin to the inactivated virus stabilized its immunogenic properties during long-term storage. It was shown that vaccine preparations with human serum albumin (0.1–1%) show higher dose-dependent stability upon repeated freezing assessed by detecting viral RNA level and their immunogenicity. It can be assumed that human serum albumin provides better preservation of of viral genetic material during storage, as well the protein epitopes responsible for the protective immune response in the form of neutralizing antibodies induction, undergo less pronounced conformational changes in the presence of albumin. These data support the addition of human serum albumin to the vaccine formulation.
oai:iimmun.ru:article/659
2022-04-12T15:45:52Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/659
2022-04-12T15:45:52Z
Russian Journal of Infection and Immunity
Vol 9, No 2 (2019); 375-380
Characteristics of acute diarrhea adult cases with samples positive for Norwalk virus
Kozhukhova E.A.; Pavlov First Saint-Petersburg State Medical University
Gorbova I.V.; S.P. Botkin Clinical Infectious Diseases Hospital
2019-05-13 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/659
острая диарея; культуральный метод; ИФА-метод; ПЦР-метод; норовирус; ассоциация энтеропатогенов
ru
Remaining unmanageable,Norwalkvirus infection is clearly tended to be recorded at higher rate, including adult patients. In many cases, clinical picture of adult acute diarrhea in patients positive for Norwalk virus in clinical samples vs. pathogenetically-caused norovirus infection differs, thereby justifying comparison of clinical and laboratory data. A cohort retrospective study with 146 hospitalized adult patients suffering from acute moderate diarrhea positive for fecalNorwalkvirus was performed. Along with standard laboratory tests (culturing, serologic for detecting 4-fold increase in titer between paired serum samples, ELISA), detection of diarrhea-linked agents included PCR kit Amplisens® AII-bacto-screen-FL Lab (Interlabservice). The data obtained demonstrated that in adult patients with acute diarrhea 54.1% of cases were positive forNorwalkvirus as well as for other enteric pathogens, including bacteria found in 36.3% of cases. Moreover, clinical samples of patients with acute diarrhea hospitalized at least on day 4 vs. day 3 after the onset were significantly more often (by 1.5-fold) positive for association between Norwalk virus and other acute enteric infection agents mainly due to astrovirus (р = 0.03; PCR data) and Shigella spp. (culture-based and serologic methods; р = 0.03). In addition,Norwalkvirus was associated with rotavirus, but not other enteric pathogens, at 2.1-fold higher rate in clinical samples from patients treated vs. untreated with antimicrobials before hospitalization. Finally, clinical samples positive for Norwalk virus from patients with vs. without developed colitis syndrome were at higher risk of developing virus-bacterial enteric infection detecting Salmonella spp. at 7.6and 3-fold higher rate verified by culture-based and PCR assay, respectively. Importantly, patients with vs. without hemorrhagic colitis Salmonella spp. verified primarily a culture-based method was detected by 11-fold more frequently (p = 0.01).
oai:iimmun.ru:article/1341
2022-04-12T15:54:48Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/1341
2022-04-12T15:54:48Z
Russian Journal of Infection and Immunity
Vol 11, No 1 (2021); 184-190
Clinical and microbiological aspects of <i>Enterococcus faecalis</i> -associated urinary tract infection
https://iimmun.ru/iimm/article/download/1341/7255
https://iimmun.ru/iimm/article/download/1341/7256
https://iimmun.ru/iimm/article/download/1341/7257
https://iimmun.ru/iimm/article/download/1341/7258
https://iimmun.ru/iimm/article/download/1341/7259
https://iimmun.ru/iimm/article/download/1341/7260
https://iimmun.ru/iimm/article/download/1341/7261
https://iimmun.ru/iimm/article/download/1341/9509
Zaitseva E.A.; Pacific State Medical University
Luchaninova V.N.; Mechnikov North-Western State Medical University
Melnikova E.A.; Regional Children's Clinical Hospital№1
Komenkova T.S.; Pacific State Medical University
Krukovich E.V.; Pacific State Medical University
2021-02-28 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1341
инфекция мочевой системы; дети; E. faecalis; биологические свойства; лабораторные показатели; клинические симптомы
Исследование выполнено при финансовой поддержке внутривузовских грантов (Приказ №105-ОД от 25.03.2015; № 61-ОД от 11.03.2016), РФФИ в рамках научного проекта № 19-315-90036
ru
Urinary tract infections (UTIs) pose a topical problem in current pediatrics, pediatric nephrology and urology. UTI-related clinical picture in childhood is polymorphic, sometimes being rather subtle and undergoing age-related alterations. Often, typical UTI symptoms in infants and early children occur subclinically. Microbe-related properties colonizing renal tissues dominate among multiple factors involved in developing UTI. In recent years, etiological importance of Enterococcus faecalis in development of such pathology has been increased. Our study was aimed to determine E. faecalis-associated UTI clinical signs in children and unveil their biological characteristics to assess related clinical significance.Materials and methods. A nine-year pediatric UTI etiological pattern was analyzed at the multi-field pediatric clinical hospital. The data of clinical and laboratory examination of 181 UTI children aged 3 days — 17 years as well as microbiological study of 60 E. faecalis strains isolated from patient urine were obtained.Results. Clinical and laboratory characteristics of E. faecalis-associated UTIs, age-related symptom variability were presented. Intoxication syndrome and fever dominated in the clinical picture. A key sign of UTIs was gastrointestinal dysfunction (in neonates and one-year old children) and pain in the lumbar region (in older children). The identified clinical symptoms may be associated with the upper urinary tract damage, concomitant diseases, and the pathogenic properties of E. faecalis. Other symptoms were less common, consistent with the age of the patients, although abdominalgia was equally common for children in all age groups. Data of laboratory examination also depended on patient age. It was noted that leukocytosis and thrombocytosis were more prominent in neonates, whereas leukocyturia and proteinuria — in children above one year of age, although clinical symptoms in this group were less overt. Specific features and clinical significance of E. faecalis-related biological properties, their heterogeneity related to patient age were noted. An inter-connected relationship between pathogenic properties and certain clinical symptoms was revealed.Conclusion. The dominant clinical symptoms (intoxication, hyperthermia), indicative of damaged upper urinary system is related, among other things, to the set of E. faecalis biological properties exerting tissue-damaging and cytolytic effects.
oai:ojs.iimmun.ru:article/253
2016-02-16T10:59:48Z
iimm:SHORT
oai:iimmun.ru:article/149
2022-04-12T21:20:53Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/149
2022-04-12T21:20:53Z
Russian Journal of Infection and Immunity
Vol 3, No 4 (2013); 371-375
ASSOCIATION BEETWEEN IL-28β POLYMORPHISMS AND EFFECTIVENESS OF CONCOMITANT THERAPY OF HIV INFECTION IN PATIENTS WITH HCV/HIV CO-INFECTION DURING THE ANTIVIRAL THERAPY
Kovaleva E.S.; State Educational Institution of Higher Professional Education Volgograd State Medical University, Volgograd
2014-07-09 00:00:00
Authors who publish with this journal agree to the following terms:
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Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/149
ВИЧ-инфекция; вирус гепатита C; сопутствующая терапия ВИЧ; эффективность лечения; полиморфизм IL-28β
ru
Abstract. The aim of the study was to evaluate the associations between the polymorphism rs8099917 IL-28β and changing of HIV viral load and the effectiveness of high active antiretroviral therapy during the treatment of chronic hepatitis C in HIV-infected patients. 80 HIV/HCV-infected patients were recruited in the study. Antiviral therapy was performed with pegylated interferon-α and ribavirin combined with concomitant HIV therapy. Polymerase chain method was used for the gene polymorphism IL 28b (rs8099917) and HIV viral load estimation. More than half (56.25%) patients treated with combined therapy showed a significant decrease in CD4 T-lymphocytes in TG- and TT-genotype groups. Furthermore, viral load of HIV RNA significantly declined in the group with TT genotype during the treatment. It was concluded that rs8099917 polymorphism of the gene IL-28β affects the dynamics of the HIV RNA viral load in HIV-infected patients receiving concomitant therapy for treatment of viral hepatitis C. Herewith, rs8099917 polymorphism combined with other factors can be used as a predictor of high active antiretroviral therapy effectiveness.
oai:iimmun.ru:article/484
2022-04-12T15:19:50Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/484
2022-04-12T15:19:50Z
Russian Journal of Infection and Immunity
Vol 7, No 1 (2017); 91-96
PERSPECTIVE OF IN VITRO LYMPHOCYTES ANTIGENICITY EVALUATION FOR THE DIAGNOSTICS OF ACUTE BRUCELLOSIS
Kostyuchenko M.V.; Stavropol Plague Control Research Institute
Ponomarenko D.G.; Stavropol Plague Control Research Institute
Rakitina E.L.; Stavropol Plague Control Research Institute
Logvinenko O.V.; Stavropol Plague Control Research Institute
Sannikova I.V.; Stavropol State Medical University of Minzdrav of Russia
Dejneka D.A.; Stavropol State Medical University of Minzdrav of Russia
Golub O.G.; City Clinical Hospital of Stavropol
2017-03-28 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/484
бруцеллез;проточная цитофлуориметрия;антигенреактивность лимфоцитов;диагностика острого бруцеллеза;тест антигенной стимуляции клеток;маркеры активации лимфоцитов
ru
The brucellosis remains to one of the most urgent dangerous infections in regions with developed livestock production. An exclusive polymorphism of symptoms, variety of forms of a disease, small informational content of results of routine laboratory all-clinical inspection, quite often leads to diagnostic mistakes at a pre-hospital stage. Improvement of a complex of laboratory diagnosis of a brucellous infection demands development of the modern padding methods of verification based on cell-like factors of immunity as leaders in an immunogenesis and a pathogenesis of a brucellosis. Considering the leading role of cell-like immunity in formation of protection against the majority of bacteriemic especially dangerous infections, studying of cell-like reaction in response to antigenic stimulation, it is necessary to consider the most informative (marker) and objective at assessment of immunologic reorganization of an organism at a disease or vaccination. The following markers (receptors) of activation of lymphocytes can act as perspective indexes of a specific cell-like antigenreactivity: CD25 — a high-affine receptor of interleukin 2 (IL-2Ra), a marker of early activation of Tlymphocytes; HLA-DR — an antigen of the main complex of a histocompatibility of a class II, an expression of a marker is associated not only with late, but also long-lived activation of lymphocytes; CD95 (Fas, APO-1) — a receptor of an induction of an apoptosis (“cell death”), a marker of “late” activation (CD4+ lymphocytes is presented mainly) and Fas L (CD178) — a receptor of an induction of an apoptosis, expresses generally on CD8+ cages. The work purpose — to estimate an opportunity and prospects of use of technology of a flowing cytofluorometry and the in vitro cell tests for diagnosis of a acute brucellosis. 35 people with the diagnosis “Acute brucellosis” and 12 people — not the patients who did not have a brucellosis, are not vaccinated against a brucellosis (control group) participated in a research. Blue blood served as material of a research. Defined quantity of the lymphocytes expressing receptors of CD25, HLA-DR, CD95, CD95L (CD178) at activation by a specific antigen. The received results processed statistically with use of Microsoft Excel 2010. During the research it is established that intensity an antigen-stimulated activation of lymphocytes of in vitro, it is possible to use as a marker of a acute brucellous infection at the person. The most perspective indexes of activation of lymphocytes of in vitro, it is possible to consider receptors to IL-2 (CD25) and apoptosis markers — CD95, CD95L (CD178). Thus, the conducted researches indicate an opportunity and the actual prospect of use of technology of a flowing cytofluorometry and cell-like in vitro tests for diagnostics of a acute brucellosis.
oai:iimmun.ru:article/719
2023-08-06T17:11:46Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/719
2023-08-06T17:11:46Z
Russian Journal of Infection and Immunity
Vol 9, No 5-6 (2019); 800-804
Importance of Epstein–Barr virus infection in pathogenesis of proliferative complications in children with endogenous uveitis
https://iimmun.ru/iimm/article/download/719/3905
https://iimmun.ru/iimm/article/download/719/3906
https://iimmun.ru/iimm/article/download/719/3907
https://iimmun.ru/iimm/article/download/719/3908
https://iimmun.ru/iimm/article/download/719/3909
https://iimmun.ru/iimm/article/download/719/3910
Petrovskaya M.S.; Helmholtz National Medical Research Centre of Eye Diseases
Krichevskaya G.I.; Helmholtz National Medical Research Centre of Eye Diseases
Denisova E.V.; Helmholtz National Medical Research Centre of Eye Diseases
Katargina L.A.; Helmholtz National Medical Research Centre of Eye Diseases
Kulikova I.G.; Helmholtz National Medical Research Centre of Eye Diseases
2020-01-31 20:11:50
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/719
вирус Эпштейна–Барр; увеит; пролиферативный синдром; IL-6; IL-8
ru
Endogenous uveitis (EU) in children is a multifactorial sight-threatening disease that reduces patient’s quality of life. Proliferative syndrome (PS) coupled to developing adhesions, opacity of vitreous body, epiretinal and preretinal membranes is one of the most serious EU complications, with yet-unknown pathogenesis. Among the numerous trigger factors, a role for infections, particularly human herpes group-driven, is proposed. The goal of the study was to assess a potential role of Herpes simplex viruses type 1 (HSV-1), Herpes simplex viruses type 2 (HSV-2), Epstein–Barr virus (EBV) and Cytomegalovirus (CMV) played in the PS pathogenesis in children with endogenous uveitis. 112 patients aged 3–17 years (mean age 10 years) with (93 patients)/without PS (19 children) were examined. IgM and IgG antibodies (markers of chronic and active infection) against HSV-1/2, EBV and CMV were detected by ELISA. A significantly increased PS rate in infected vs. uninfected children was revealed solely for EBV infection (p = 0.03), but not for HSV-1/2 (p > 0.05) or CMV-positive patients (p > 0.05). However, PS emergence in EBV-negative patients also suggests that some factors might contribute to proliferation in intraocular inflammation. In addition, level of serum IL-8 and IL-6 were assessed by multiplex analysis in 28 children. It was found that IL-8 was detected in all patients, with great individual fluctuations (5.6–2743 pg/ml). Enhanced systemic IL-8 level tended to rise in patients with more prominent proliferation and serological markers of EBV reactivation. However, serum IL-6 was detected by about 2-fold less often reaching up to 55% cases (variation of individual indices 1.3–35.5 pg/ml). A correlation between PS severity, EBV infection activity and systemic IL-6 level was not observed. Further studies evaluating a role of EBV infection in PS pathogenesis pediatric endogenous uveitis are necessary, as it may underlie a rationale for including antiherpetic drugs into a combination therapy.
oai:iimmun.ru:article/44
2022-04-12T21:19:45Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/44
2022-04-12T21:19:45Z
Russian Journal of Infection and Immunity
Vol 1, No 2 (2011); 177-180
CHARACTERISTIC OF SENSITIVITY OF STAPHYLOCOCCUS AUREUS AND CANDIDA ALBICANS TO ANTIBACTERIAL PREPARATIONS AND COLLOIDAL SILVER
Afonina I.A.; ФГУН «Санкт-Петербургский научно-исследовательский институт эпидемиологии и микробиологии имени Пастера» Роспотребнадзора, Санкт-Петербург
Kraeva L.A.; ФГУН «Санкт-Петербургский научно-исследовательский институт эпидемиологии и микробиологии имени Пастера» Роспотребнадзора, Санкт-Петербург
Tseneva G.Y.; ФГУН «Санкт-Петербургский научно-исследовательский институт эпидемиологии и микробиологии имени Пастера» Роспотребнадзора, Санкт-Петербург
2014-06-28 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/44
коллоидное серебро; Staphylococcus aureus; Candida albicans; противомикробные препараты
ru
Abstract. Constant use of antibiotics leads to reliable increasing of resistance among microorganisms. Using non-toxic concentrations of colloidal silver in combination with antimicrobial agents can reduce using concentrations of antibiotics, kept necessary antimicrobial effect. In case of Staphylococcus aureus bactericidal activity of the complex of colloidal silver with unit concentration of neomycin is bigger than the bactericidal effect of double concentration of the antibiotic. Fungicidal effect of combination of antifungal agents with a solution of the colloidal silver on Candida albicans is equal to fungicidal effect of double concentration of antifungal drugs.
oai:iimmun.ru:article/277
2022-04-12T15:14:20Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/277
2022-04-12T15:14:20Z
Russian Journal of Infection and Immunity
Vol 5, No 1 (2015); 79-84
CLINICAL SIGNIFICANCE OF CYTOKINES SERUM LEVELS IN CHILDREN WITH CHICKEN POX
Zheleznikova G.F.; 195267, Russian Federation, St. Petersburg, Acad. Konstantinov str., 12/80. Science Research Institute of Children’s Infections
Lobzin Y.V.; 195267, Russian Federation, St. Petersburg, Acad. Konstantinov str., 12/80. Science Research Institute of Children’s Infections
Skripchenko N.V.; 195267, Russian Federation, St. Petersburg, Acad. Konstantinov str., 12/80. Science Research Institute of Children’s Infections
St. Petersburg State Pediatric Medical University, St. Petersburg, Russia
Ivanova G.P.; 195267, Russian Federation, St. Petersburg, Acad. Konstantinov str., 12/80. Science Research Institute of Children’s Infections
St. Petersburg State Pediatric Medical University, St. Petersburg, Russia
Skripchenko E.Y.; St. Petersburg State Pediatric Medical University, St. Petersburg, Russia
City Clinical Hospital No. 31, St. Petersburg, Russia
Monakhova N.E.; 195267, Russian Federation, St. Petersburg, Acad. Konstantinov str., 12/80. Science Research Institute of Children’s Infections
2015-04-21 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/277
цитокины;ветряная оспа;ветряночный энцефалит;дети
ru
The serum levels of cytokines IL-1β, IL-8, IL-6, IFNα, IFNγ, IL-4, IL-10 were tested in ELISA in 74 children with different courses of chicken pox. Moderate severity course of chicken pox was accompanied by significant increase of IL-1β, IL-8, IFNα, IFNγ, IL-10 levels, but severe course of infection was associated with cytokine response reduction. Manifestation of varicella-zoster virus encephalitis was accompanied by the rise of IL-1β, IL-6, IFNγ and IL-10 levels during the second week of disease.
oai:iimmun.ru:article/1393
2023-08-06T17:09:27Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/1393
2023-08-06T17:09:27Z
Russian Journal of Infection and Immunity
Vol 11, No 5 (2021); 973-978
Parameters of blood short-chain peptides and digestive hydrolases in patients with chronic hepatitis B virus infection
https://iimmun.ru/iimm/article/download/1393/7860
https://iimmun.ru/iimm/article/download/1393/7859
https://iimmun.ru/iimm/article/download/1393/8880
https://iimmun.ru/iimm/article/download/1393/8881
https://iimmun.ru/iimm/article/download/1393/8882
https://iimmun.ru/iimm/article/download/1393/8883
https://iimmun.ru/iimm/article/download/1393/8884
https://iimmun.ru/iimm/article/download/1393/8885
https://iimmun.ru/iimm/article/download/1393/9027
https://iimmun.ru/iimm/article/download/1393/9028
https://iimmun.ru/iimm/article/download/1393/9029
https://iimmun.ru/iimm/article/download/1393/9030
https://iimmun.ru/iimm/article/download/1393/9031
https://iimmun.ru/iimm/article/download/1393/9032
https://iimmun.ru/iimm/article/download/1393/9033
https://iimmun.ru/iimm/article/download/1393/9034
https://iimmun.ru/iimm/article/download/1393/11362
Zhuraeva M.A.; Andijan State Medical Institute
Aleinik V.A.; Andijan State Medical Institute
Ashuralieva N.D.; Andijan State Medical Institute
Halikova D.S.; Andijan State Medical Institute
Ravzatov J.B.; Andijan State Medical Institute
Abdulazizhozhyv B.K.; Andijan State Medical Institute
2021-07-13 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1393
хронический гепатит B; сывороточные гидролазы желудка; сывороточные гидролазы поджелудочной
железы; панкреатит; атрофический гастрит
ru
The aim of the study was to examine specifics of changes in the level of stomach- and pancreas-released blood hydrolases in chronic viral hepatitis B and analyze the mechanisms underlying such changes. We assessed serum markers of HBV infection, liver enzymes tests as well as gastric and pancreatic hydrolase level. The patients examined were divided into three groups: control (healthy) and two study groups — HBV post-infection and chronic HBV infection. Patients with HBV post-infection had no significant deviations from normal range for blood level of gastric and pancreatic hydrolases. Patients with chronic HBV infection were found to contain increased blood level of amylase and lipase, which may evidence about increasing pancreatic functional activity and development of covert pancreatitis. At the same time, decline in the concentration of serum pepsinogen-1 below 40 μg/l could indicate about prominently decreased secretion of hydrochloric acid and development of atrophic gastritis, and it was found that the major factor contributing to development of such disorders was the short-chain peptide CCK-8, which utilization declines in patients with chronic HBV infection. CCK-8 can play a pivotal role in inhibiting stimulation of gastric acid secretion and controls gastric acid, plasma gastrin and somatostatin secretion. Cholecystokinin has been found to inhibit acid secretion by activating CCK type A receptors as well as via somatostatin-involving mechanism. The secretion of gastric somatostatin-14 increased by fivefold due to CCK-8 alone, but was blocked by the CCK-A receptor blocker loxiglumide. These data show that CCK-8 directly inhibits acid reactions by stimulating the release of gastric somatostatin indirectly through the CCK-A receptor. Thus, it can be assumed that normally CCK-8 is mainly utilized by the liver, which is altered during chronic hepatitis B resulting in elevated blood CCK-8 concentration. As a consequence, it enhances pancreatic secretion resulting in developing pancreatitis that is paralleled with inhibited gastric secretion and emerged atrophic gastritis.
oai:iimmun.ru:article/2038
2023-04-01T17:51:48Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/2038
2023-04-01T17:51:48Z
Russian Journal of Infection and Immunity
Vol 13, No 1 (2023); 161-166
The features of developing rat autoimmune pathology with mitochondrial dysfunction
https://iimmun.ru/iimm/article/download/2038/14494
https://iimmun.ru/iimm/article/download/2038/14497
https://iimmun.ru/iimm/article/download/2038/14498
https://iimmun.ru/iimm/article/download/2038/14500
https://iimmun.ru/iimm/article/download/2038/14721
https://iimmun.ru/iimm/article/download/2038/14722
https://iimmun.ru/iimm/article/download/2038/14723
https://iimmun.ru/iimm/article/download/2038/15011
https://iimmun.ru/iimm/article/download/2038/74121
Skupnevskiy S.V.; Institute of Biomedical Investigations — the Affiliate of Vladikavkaz Scientific Centre of RAS
Pukhaeva E.G.; Institute of Biomedical Investigations — the Affiliate of Vladikavkaz Scientific Centre of RAS
Badtiev A.K.; Institute of Biomedical Investigations — the Affiliate of Vladikavkaz Scientific Centre of RAS
Rurua F.K.; Institute of Biomedical Investigations — the Affiliate of Vladikavkaz Scientific Centre of RAS
Batagova F.E.; Institute of Biomedical Investigations — the Affiliate of Vladikavkaz Scientific Centre of RAS
Farnieva Z.G.; Institute of Biomedical Investigations — the Affiliate of Vladikavkaz Scientific Centre of RAS
2023-04-01 18:41:47
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/2038
адъювант Фрейнда; аутоиммунный ревматоидный артрит; иммунопатологический процесс; купризон; механизм аутоиммунной патологии; митохондриальная дисфункция
Array
ru
The central role of the mitochondria in energy supply and cell death determines highlight these organelles as one of the promising objects for investigating pathogenesis of immune-mediated inflammatory disorders. The aim: to study features of pathogenesis in rat adjuvant-induced autoimmune pathology separately and in combination with mitochondrial disorders. Materials and methods. Wistar rats were divided into groups of negative control (solvent), positive control (single subcutaneous injection of complete Freund’s adjuvant (CAF) at dose of 0.1 ml/200 g body weight), experimental (CAF 0.1 ml/200 g body weight and 5 weeks later — with cuprizone 0.2% per feed weight). At the end of experiment (7 weeks), animals were tested in the “open field” model, euthanized, and biomaterial was collected to measure the relative mass coefficients of internal organs, hematological and histological studies. We calculated the mean, standard error of the mean; comparison of hypotheses was carried out by paired Student’s t-test. Results. In case of impaired immunological tolerance there was detected reduced rat body weight gain during the study period (negative control +74.7 g, positive control +10.3 g) along with modelled mitochondrial dysfunction, a general decrease in weight by 6.7 g was noted. The magnitude of mass coefficients indicate a relative reduction in mass of liver, kidneys, spleen and thymus in experimental animals. The leukocyte counts (× 109/L) are as follows: negative control — 8.68±0.37, positive control — 10.98±1.03 (p < 0.05), experimental group — 12.28±0.63 (p < 0.001). No significant changes were found in the leukocyte formula and the red cell lineage. During modelled autoimmune pathology, platelet count increased by 22.5% (p < 0.05), whereas after cuprizone was administered it decreased by 6.3% (relative to the negative control). Mitochondrial dysfunction caused an abrupt decrease in motor activity in rats: the number of crossed sectors in positive control animals was 55.50±6.91, experimental group — 44.50±3.60 (inter-group comparison, p < 0.001). Positive control: enlarged lymphatic nodules were found in the spleen, germinal center clarification, wall thickening of the pulpal and central arteries; single foci of hemorrhages in the red pulp. Experimental group: atrophy of lymphoid follicles of varying severity (relative to the groups of negative and positive controls), numerous foci of hemorrhages with hemosiderosis in the red pulp. Conclusion. Mitochondrial dysfunction is accompanied by augmented pathogenetic signs of autoimmune pathology, which can serve as one of the keys to understanding the mechanisms of human autoimmunity.
oai:iimmun.ru:article/791
2023-08-06T17:26:39Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/791
2023-08-06T17:26:39Z
Russian Journal of Infection and Immunity
Vol 8, No 3 (2018); 383-387
IMPACT OF COINFECTION OF PV B19 ON THE COURSE AND PROGNOSIS OF MALARIA CAUSED BY PLASMODIUM FALCIPARUM
Lavrentyeva I.N.; St. Petersburg Pasteur Institute.
Khamitova I.V.; St. Petersburg Pasteur Institute.
Slita A.V.; St. Petersburg Pasteur Institute.
Levkovski A.E.; Hospital RUSAL FRIGUIA.
Diallo A.A.; Hospital RUSAL FRIGUIA.
Diallo A.K.; Hospital RUSAL FRIGUIA.
Sow T.C.; Hospital RUSAL FRIGUIA.
Naydenova E.V.; Russian Research Anti-Plague Institute “Microbe”.
Agafonov D.A.; Russian Research Anti-Plague Institute “Microbe”.
Senichkina A.M.; Russian Research Anti-Plague Institute “Microbe”.
2018-11-02 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/791
парвовирусная инфекция; ДНК парвовируса В19; Plasmodium falciparum; малярия; анемия
ru
Parvovirus infection (PVI) is widespread in the world; more than 80% of the adult population have antibodies of IgG class to parvovirus B19. Malaria is a vector-borne parasitic disease caused by the protozoa of the genus Plasmodium, that is widespread in the countries of Africa, Southeast Asia, Oceania. The objective of the present study was to evaluate the effect of parvovirus B19 infection on the clinical course of malaria and the outcome of the underlying disease. During the period 2016–2018 blood plasma samples of 316 patients from the hospital of the Friya Prefecture of the Republic of Guinea (GR) with confirmed diagnosis of malaria were examined for the presence of PVB19 DNA. The clinical course of malaria in 316 examined patients was divided into group of either mild or complicated. In total, PVB19 DNA was detected in blood plasma in 55 of 316 patients (17.41±2.13%). But in the group with co-infection of PVB19 and P. falciparum complications were observed in 40 of 55 (72.73±2.75%) patients, and in 6 of 55 cases (10.91±4.40%) the disease resulted in death. In the group of patients with malaria without PVI, complications occurred in 99 of 261 patients (37.9±3.0%); of those 2 (0.77±0.54%) died. It was found that the most numerous group in the structure of malaria patients is represented by children under 5 (median 3) years (89, or 28.25±2.53%). Our results correlate with the data of other researchers who studied the PVI-associated malaria in children in malaria-endemic regions: among children under 5 years, the absolute majority of cases of PVI was accompanied by a complicated course of malaria. The primary parvovirus infection can aggravate the course of malaria, especially when combined with other unfavorable conditions (iron deficiency, malnutrition, helminthic infections, co-infections, etc.). Thus, infection with PVB19 becomes a critical factor, which can provoke a severe life-threatening anemia, and also cause other complications.
oai:iimmun.ru:article/1221
2023-08-06T17:10:22Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/1221
2023-08-06T17:10:22Z
Russian Journal of Infection and Immunity
Vol 10, No 4 (2020); 782-788
Comparative prevalence of atopic sensitization in the border areas between Russia and Finland
Markelova O.A.; Petrozavodsk State University
Vezikova N.N.; Petrozavodsk State University
Zilber E.K.; St. Petersburg State Research Institute of Phthisiopulmonology of the Ministry of Healthcare of the Russian Federation
2020-11-26 00:00:00
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url:https://iimmun.ru/iimm/article/view/1221
атопия; распространенность; динамика атопии; Россия; Финляндия; аллергические заболевания
ru
Current century has been highlighted with rise in allergic pathology, and environment markedly affects it via epigenetic mechanisms. Allergy research in Russia and Finland has been conducted for many years, of larger scale recorded in the latter, still being maintained over time. Atopy prevalence was studied in the border areas in both countries. The level of total, dietary, inhaled IgE level, as well as dietary and inhalation range were examined. The Phadiatop® radio allergen sorbent test was used (UniCAP 1000 v.2; Pharmacia Upjohn, Uppsala, Sweden), with cut-off point: IgE > 110 kUA/l and sIgE > 2.5 kUa/l and 0.35 kUA/l. The study was subdivided into 2 stages conducted during 2003 and 2010/2012 timeframe. Stage 1 was performed with children from Finland (n = 344), Russia (n = 427), aged 7—15 years, whereas stage 2 — 180 subjects (Finland, n = 98, Russia, n = 82). Results. It was found that total IgE level was higher in children from Finland. The inhaled sIgE in 2003 and 2010/2012 was higher among children from Finland, p = 0.019 and p = 0.027, showing temporal elevation. In 2003, dietary sIgE was higher among those from Finland (27.6% and 18.3%), p = 0.142, whereas in 2010/2012 it was decreased by 2- and 3-fold in subjects from Finland and Russia, respectively. In 2003, high atopy rate against dust mites, cats, timothy, birch, and dogs was found in Russia, which was increased at stage 2. Such parameter was most evidently elevated for timothy — by 2-fold, p = 0.176, wormwood — by 2-fold, p = 0.312, equine allergens — by 2-fold, p = 0.563, canine allergens — by 1.5-fold, mite — by 1.5-fold, p = 0.220. In 2003, atopy rate in Finland to canine allergens reached 26.5%, timothy — 24.5%, birch — 20.4%, feline allergens — 20.4%. In 2010, it was noted rise in atopy rate mainly to dust mites — by 2-fold, equine allergens — by 2-fold. In 2003, atopy rate in Russia to egg, milk, and wheat prevailed, whereas in Finland it was mostly found against milk, nuts, wheat, eggs, in both cases declined in dynamics. Interestingly, no sensitization to fish in subjects from Russia and Finland was observed at both stages. Finally, at the stage 2 dietary atopy rate was declined in both countries. Differences in atopic sensitization as well as during 7—9 year follow-up observation were preserved between both subject cohorts from Finland and Russia.
oai:iimmun.ru:article/144
2022-04-12T21:20:53Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/144
2022-04-12T21:20:53Z
Russian Journal of Infection and Immunity
Vol 3, No 4 (2013); 347-350
THE ACCELERATED METHOD FOR IDENTIFICATION OF PATHOGENIC CORYNEBACTERIUM
Gabrielyan S.A.; National Public Health Institute of Armenia Ministry of Health, Yerevan
2014-07-08 00:00:00
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Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/144
коринебактерии; цистиназная активность; модифицированная среда
ru
Abstract. The detection of cystinase activity is one of most important tests in the laboratory diagnosis of diphtheria, giving the opportunity to differentiate potential toxigenic species from other coryneform bacteria. The original recipe of Pizu media for detection of cystinase activity, proposed in 1939–1940, was modified several times (1982, 1989) for different reasons. In the last modification the Pizu media was prepared by using the AGV media as a nutritional base. We suggest a modification of Pizu media using Mueller-Hinton agar as nutritional basis. The Mueller-Hinton agar has a number of advantages: higher nutritional value, standardization, transparency and availability. A positive result is defined within 2–4 hours after inoculation of enough quantity of material (pure culture or C. diphtheriaе in association with other microorganisms) as a brown halo surrounding black colonies. The brown halo does not appear in the upper part of the media (0,5–1 cm). The modified media was checked with 21 strains of C. diphtheriaе (tox+), nine strains of coryneform bacteria, control strains NCTC 10648, NCTC 10356, NCTC 3984. The modified Pizu media is registered in the RA mental property agency as an invention (no. 1877 A2, 15.12.2006).
oai:iimmun.ru:article/451
2023-08-06T17:26:48Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/451
2023-08-06T17:26:48Z
Russian Journal of Infection and Immunity
Vol 6, No 4 (2016); 379-383
OPEN ENDED RESULTS OF ACUTE DIARRHEA AGENT DETECTION TO VERIFY DIAGNOSIS IN ADULT CASES
Kozhukhova E.A.; Pavlov First St. Petersburg State Medical University, St. Petersburg, Russian Federation
Andreeva N.V.; Hospital named after S.P. Botkin for Infectious Diseases in Adults, St. Petersburg, Russian Federation
Ivaschenko V.D.; Pavlov First St. Petersburg State Medical University, St. Petersburg, Russian Federation
2017-01-06 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
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Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/451
острая диарея; ассоциация энтеропатогенов; культуральный метод; иммуноферментный анализ; полимеразная цепная реакция
Array
ru
In 264 adult acute diarrhea cases with moderate course it was analyzed both symptoms and agents detected by different methods: in 91 cases (examined in the period of shigellosis high incidence level) — by culture and serologic (specific antibodies detection) methods to detect Shigella spp. and Salmonella spp. plus ELISA method to detect rotavirus antigen in feces; in 173 cases (examined in the period of shigellosis low incidence level) — by above mentioned methods plus PCR based method. Data obtained in the period of shigellosis high incidence level have shown that there has been no significant difference in clinical scores (including colitis frequency) between cases positive only for rotavirus antigen and those positive both for rotavirus antigen and Shigella spp. culture. That let suspect that acute diarrhea had been likely to be caused by association of rotavirus with any bacterial agent undetected by the methods used. PCR-based diagnostic method additionally used (in the period of shigellosis low incidence level) resulted in detection of Campylobacter spp. accounted for campilobacteriosis as mono infection in 20,8% cases and as mixed infection (in association with other enteropatho-gens) — in 4% cases. In cases with colitis the additional usage of PCR-based diagnostic method resulted in Salmonella spp. and Campylobacter spp. detection in every third case, Shigella spp. and agent association detection — in every fifth case. The target analysis of enteropathogens detected in 15 cases positive for rotavirus (examined in the period of shigellosis low incidence level) has shown that in 6 of them there has been detected association of rotavirus with other agents predominantly bacterial ones (in 5 of 6 cases). So, the PCR method might be quite useful to broaden the spectrum of detected enteropathogens in adult acute diarrhea cases especially in those with colitis syndrome available.
oai:iimmun.ru:article/1771
2023-08-06T17:08:25Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/1771
2023-08-06T17:08:25Z
Russian Journal of Infection and Immunity
Vol 12, No 3 (2022); 575-579
Enhanced hydroxyl radical generation by human leukocytes exposed to bacterial diamines highlighting immunomodulatory effect of microbial metabolites
https://iimmun.ru/iimm/article/download/1771/11407
https://iimmun.ru/iimm/article/download/1771/11408
https://iimmun.ru/iimm/article/download/1771/11409
https://iimmun.ru/iimm/article/download/1771/11410
https://iimmun.ru/iimm/article/download/1771/11411
https://iimmun.ru/iimm/article/download/1771/11412
https://iimmun.ru/iimm/article/download/1771/11417
https://iimmun.ru/iimm/article/download/1771/12747
https://iimmun.ru/iimm/article/download/1771/13940
Godovalov A.P.; E.A. Vagner Perm State Medical University
Karpunina T.I.; E.A. Vagner Perm State Medical University
Morozov I.A.; E.A. Vagner Perm State Medical University
2022-07-04 15:06:39
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1771
кадаверин; путресцин; лейкоциты; гидроксильные радикалы; хемилюминесценция; микроорганизмы
Array
ru
Recently, there have been increasing an interest to study a role of polyamines in intercellular interactions, especially in the focus of inflammation, where accumulation of such polycations is observed. In this regard, products of microbial origin — cadaverine and putrescine — are of particular attention. The role of polyamines as «scavengers» of free radicals has been described, but no data of their effect on the leukocyte radical-producing activity have been obtained so far. The aim of the investigation was to study features of hydroxyl radical generation by human leukocytes exposed to microbe-derived polyamines. Materials and methods. Peripheral venous blood samples were obtained from 20 healthy donors. To assess radical production, a luminol-dependent chemiluminescence reaction was carried out with blood leukocytes pre-incubated with cadaverine (0.01 M) and putrescine (0.01 M), measured on a Luminoskan Ascent® Thermo Labsystems luminometer (USA) for 180 min. For statistical analysis, an integral chemiluminescence index was used for the entire measurement period (RLU). Results and discussion. It was shown that cadaverine has a stimulating effect on the leukocyte potential to produce radicals (averaged area under the curve is 6.7±0.7 r.u., p < 0.05). Putrescine had little effect on the radical-producing activity of human cells (2.8±0.4 r.u., p < 0.05). This might be due to the direct influence of polycations on the mechanisms of radical generation, as well as increased activity of leukocyte diamine oxidase catalyzing the conversion of diamines into aminoaldehyde, which is accompanied by the release of hydrogen peroxide reacting with luminol. In addition, the enzyme inactivates other compounds, such as histamine leading to formation of a less pronounced clinical picture. Polyamines, particularly cadaverine and putrescine, can be referred to the factors remodeling the metabolic activity of the host leukocytes, which is aimed at maintaining the viability and increasing the number of microorganisms. Conclusion. Thus, cadaverine and putrescine produced by microorganisms, depending on the conditions of the microenvironment, might be evidently considered as mediators of the mild or asymptomatic course of inflammatory diseases, which contributes to underlying persistent potential of bacteria.
oai:oai.iimmun.ru:article/1573
2020-08-07T07:04:12Z
iimm:SHORT
oai:iimmun.ru:article/177
2022-04-12T21:20:45Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/177
2022-04-12T21:20:45Z
Russian Journal of Infection and Immunity
Vol 3, No 3 (2013); 285-288
SEROLOGICAL DIAGNOSIS OF MODERN CHOLERA USING LIPOSOMAL ENTEROTOXIC DIAGNOSTICUM IN COMPLEMENT FIXATION TEST
Savelyeva I.V.; The Federal Government Public Health Institution “Stavropol Institute for Plague Control of the Rospotrebnadzor”, Stavropol
Bezsmertnyj V.E.; The Federal Government Public Health Institution “Anti-plague Centre of the Rospotrebnadzor”, Moscow
Savelyev V.N.; The Federal Government Public Health Institution “Stavropol Institute for Plague Control of the Rospotrebnadzor”, Stavropol
Fedorov Y.M.; The Federal Government Public Health Institution “Anti-plague Centre of the Rospotrebnadzor”, Moscow
Ivanova C.M.; The Federal Government Public Health Institution “Anti-plague Centre of the Rospotrebnadzor”, Moscow
Ivannikov V.V.; The Federal Government Public Health Institution “Anti-plague Centre of the Rospotrebnadzor”, Moscow
2014-07-11 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/177
серологическая диагностика; холера; липосомальный диагностикум; РСК
ru
Abstract. The possibility of serological diagnosis of cholera using cholera enterotoxic diagnostics kit in complement fixation test to detect anti-enterotoxic antibodies in sera of patients with cholera caused by hybrid variants of the El Tor biovar has been demonstrated. In patients with mild course of cholera anti-enterotoxic antibodies were detected in titres 1:50 and 1:200 in paired sera obtained on the 7th and 14th days of disease, respectively (fourfold titre increase). In patients with the course of medium severity 32-fold titre increase was recorded from the titre 1:100 in serum obtained on the fifth day of disease till the titre 1:3200 — on the twelfth day of disease. Antibodies titers reached 1:1600 and 1:800 were revealed in two medium course patients (adult and infant of 10 months) on the sixth day of disease.
oai:iimmun.ru:article/1253
2023-08-06T17:09:24Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/1253
2023-08-06T17:09:24Z
Russian Journal of Infection and Immunity
Vol 11, No 5 (2021); 984-988
Pseudomonas aeruginosa significantly increases expression of receptor for advanced glycation endproducts (RAGE) in the septicemia suffering patients
https://iimmun.ru/iimm/article/download/1253/6497
https://iimmun.ru/iimm/article/download/1253/6498
https://iimmun.ru/iimm/article/download/1253/6499
https://iimmun.ru/iimm/article/download/1253/6500
https://iimmun.ru/iimm/article/download/1253/10934
Kariminik A.; Islamic Azad University, Kerman Branch
Hosseini F.; Islamic Azad University, Kerman Branch
Nasiri E.; Islamic Azad University, Kerman Branch
2021-05-17 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1253
септицемия; RAGE; CXCL11; экспрессия гена
This project was supported by a grant from the Kerman Branch, Islamic Azad University, Kerman, Iran.
en
Receptor for Advanced Glycation Endproducts (RAGE) is a cell surface receptor, which recognizes several endogenous and exogenous molecules and subsequently induces expression of several molecules including chemokines. Chemokines are members of the cytokine superfamily and participate in several immune system functions, including cell migration, inflammation, angiogenesis/angiostasis etc. CXC ligand 11 (CXCL11) is an important chemokine which participates in the induction of appropriate immune responses against microbes, including bacteria. The main mechanisms responsible to overcome septicemia are yet to be clarified. Thus, it has been hypothesized that RAGE may participate in induction of CXCL11 in response to the microbial agents. Due to the fact that immune responses play key roles in limitation of infection, it has been proposed that RAGE may inhibit spread of septicemia. Therefore, in this project mRNA levels of RAGE and CXCL11 were explored in the patients suffering from septicemia versus healthy controls. RAGE and CXCL11 expression levels in the 80 subjects, including 40 septicemia patients and 40 healthy controls were explored using Real-Time PCR technique. Accordingly, by using the specific primer against RAGE and CXCL11 in a Rotorgene vehicle the mRNA levels have been determined. The septicemia and the sources of the bacteria in the blood were diagnosed using microbial cultures. The results demonstrated that although mRNA levels for RAGE and CXCL11 did not change in the septicemia patients vs. healthy controls, mRNA levels of RAGE were significantly higher in the patients infected by Pseudomonas aeruginosa compared to those infected by other bacteria, Escherichia coli, Staphylococcus aureus, and Acinetobacter baumannii. RAGE and CXCL11 mRNA levels did not differ among male and female patients. Based on the results it seems that RAGE is a critical receptor against P. aeruginosa during septicemia and more investigations, especially on the RAGE down-stream molecules can clarify its main roles against P. aeruginosa.
oai:iimmun.ru:article/2022
2023-11-19T05:27:02Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/2022
2023-11-19T05:27:02Z
Russian Journal of Infection and Immunity
Vol 12, No 6 (2022); 1156-1162
Molecular genetic characteristics of <i>Vibrio cholerae</i> nono1/nono139 strain, the causative agent of a new case of acute intestinal infection in Rostov-on-Don
https://iimmun.ru/iimm/article/download/2022/14218
https://iimmun.ru/iimm/article/download/2022/14219
https://iimmun.ru/iimm/article/download/2022/14220
https://iimmun.ru/iimm/article/download/2022/14221
https://iimmun.ru/iimm/article/download/2022/14222
https://iimmun.ru/iimm/article/download/2022/14223
https://iimmun.ru/iimm/article/download/2022/14224
https://iimmun.ru/iimm/article/download/2022/14225
https://iimmun.ru/iimm/article/download/2022/14953
https://iimmun.ru/iimm/article/download/2022/15335
https://iimmun.ru/iimm/article/download/2022/15336
Monakhova E. .; Rostov-on-Don Research Anti-Plague Institute
Kruglikov V.D.; Rostov-on-Don Research Anti-Plague Institute
Vodop'yanov A.S.; Rostov-on-Don Research Anti-Plague Institute
Selyanskaya N.A.; Rostov-on-Don Research Anti-Plague Institute
Ezhova M.I.; Rostov-on-Don Research Anti-Plague Institute
Noskov A.K.; Rostov-on-Don Research Anti-Plague Institute
2022-12-30 21:01:15
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/2022
холерные вибрионы нeО1/неО139 (НАГ-вибрионы); острые кишечные инфекции; факторы патогенности; антибиотикочувствительность; полногеномное секвенирование; биоинформационный анализ
Array
ru
During more than 50 years, a heterogeneous population of Vibrio cholerae non-O1/non-O139 (NAGs) exists in Rostov region of Russia, whose representatives periodically cause acute human intestinal infections; the last case was registered in 2018. In 2022, a sick child was again revealed in Rostov, from whom 2 NAG subcultures were isolated. Aim of the study: bioinformatics analysis of whole genome sequences (WGSs) of the 2022 clinical NAG isolates, genetic determinants of pathogenicity factors and relevant products. Materials and methods. Isolation, identification of cultures, as well as antibiotic sensitivity were carried out according to the MUK 4.2.3745-22. SNP analysis of WGSs obtained on the MiSeq Illumina platform were analyzed by means of designed software; BioEdit 7.2.5, Vector NTI Advance 11 package, CARD database were used to identify and analyze genes and relevant deduced products. Results. The isolated cultures with identical phenotypic features, PCR-genotypes and results of SNP analysis, were identified as a non-toxigenic NAG. The agent showed sensitivity to chloramphenicol, ceftriaxone, streptomycin, gentamicin, nalidixic acid, ciprofloxacin, ampicillin, amikacin and intermediate resistance to tetracycline, doxycycline, furazolidon and co-trimoxazole. WGSs lacked CTX and preCTX prophages, pathogenicity island VPI, thermostable and cholix toxin genes, pandemic islands VSP-I and VSP-II. The determinants of the type 6 secretion system (T6SS) were not presented completely, which evidenced in favor of the loss of its functionality. An intact T3SS cluster was revealed, which was adjacent to the nan-nag region of the VPI-2 pathogenicity island in the absence of the proximal and distal parts of the latter. Other determinants of pathogenicity factors were revealed: RTX cluster responsible for synthesis of MARTX cytotoxin, genes for hemolysin HlyA, cytotonic toxin Cef, several serine and metalloproteases. The nucleotide compositions of most determinants differed from the prototypes, but their putative products preserved characteristic active domains. The adhesive activity might be provided by mannose-insensitive MSHA-like pili, since the msh-cluster included all the genes necessary for their production, as well as flagella, as both clusters responsible for their assembling were 99% identical to the prototype. From antibiotic resistance determinants only β-lactamase VarG gene was found. Conclusion. The NAG strain examined here contains a sufficient set of intact virulence-associated genetic determinants, expression of which could contribute to the development of the disease.
oai:iimmun.ru:article/742
2022-04-12T15:22:30Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/742
2022-04-12T15:22:30Z
Russian Journal of Infection and Immunity
Vol 8, No 2 (2018); 223-229
DETERMINATION OF SOME IMMUNOLOGICAL FEATURES OF HHV-6-MEDIATED INFECTIOUS MONONUCLEOSIS IN CHILDREN BY THE METHOD OF DISCRIMINATORY ANALYSIS
Filatova E.N.; Blokhina Scientific Research Institute of Epidemiology and Microbiology of Nizhny Novgorod
Solntsev L.A.; Blokhina Scientific Research Institute of Epidemiology and Microbiology of Nizhny Novgorod
Presnyakova N.B.; Blokhina Scientific Research Institute of Epidemiology and Microbiology of Nizhny Novgorod
Kulova E.A.; Nizhny Novgorod State Medical Academy
Utkin O.V.; Blokhina Scientific Research Institute of Epidemiology and Microbiology of Nizhny Novgorod
Nizhny Novgorod State Medical Academy
2018-09-10 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/742
ВГЧ-6;инфекционный мононуклеоз;популяционный состав клеток крови;иммунитет;проточная цитометрия;градиентный бустинг
ru
Human herpesvirus type 6 (HHV-6) is a lymphotropic virus that is an etiological agent of infectious mononucleosis (IM) in children. HHV-6- mediated infectious mononucleosis (HHV-6M) does not have clearly defined clinical features. Nowadays immunopathogenetic aspects of this disease have not been fully understood. The purpose of this work was to study the characteristics of the quantitative composition of populations of immunocompetent cells of peripheral blood in children with HHV-6M. The material for the study was samples of peripheral blood from children with “infectious mononucleosis” diagnosis and from virtually healthy children. Depending on the etiologic cause of the disease, children with IM were divided into three groups: HHV-6M, IM of other etiology and mixed infection (combination of HHV-6 and Epstein–Barr virus and/or Cytomegalovirus). Virtually healthy children formed the fourth group. In blood samples, the absolute content of the following populations of immunocompetent cells was determined by the method of flow cytometry: the total population of T-lymphocytes, T- helpers, cytotoxic T-lymphocytes, double positive T-lymphocytes (CD4+CD8+), NK cells and B-lymphocytes. Discriminant analysis was carried out: based on the obtained data on the population composition of blood cells we constructed a model of a child’s attribution to one of the four groups analyzed in pairs. We used the method of machine learning — the algorithm of gradient boosting over decision trees. It was determined whether it is possible to classify patients on the basis of the studied indicators and which combination of indicators is optimal for classification. As a result of the study it was possible to classify the following pairs of groups: healthy children — children with HHV- 6M, healthy children — children with IM of other etiology, children with HHV-6M — children with IM of other etiology. When solving the problem of classifying children from group with mixed infection and from any other group, it was not possible to find a model of satisfactory quality. In comparison with virtually healthy children, children with HHV-6M were characterized by an increased content of the total population of T-lymphocytes and cytotoxic T-cells, as well as by a reduced content of doub le-positive T-lymphocytes. Compared with children with IM of other etiology, children with HHV-6M were characterized by an increased content of cytotoxic T-lymphocytes, T- helpers, B-lymphocytes and a reduced number of double-positive T cells. Our results indicate that HHV-6-mediated infectious mononucleosis causes changes in the quantitative composition of certain populations of immunocompetent cells of peripheral blood, different from those of other etiology, in children.
oai:iimmun.ru:article/1483
2023-08-06T17:10:56Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/1483
2023-08-06T17:10:56Z
Russian Journal of Infection and Immunity
Vol 10, No 3 (2020); 587-590
Organ-specific pathomorphological changes during COVID-19
Vorobeva O.V.; I.N. Ulianov Chuvash State University
Lastochkin A.V.; I.N. Ulianov Chuvash State University
2020-05-25 14:18:23
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1483
COVID-19; ишемическая болезнь сердца; артериальная гипертензия; пневмония
ru
COVID-19 is an acute respiratory infection caused by SARS-CoV-2 coronavirus causing pneumonia, lesions in the cardiovascular system and other organs, high mortality risk, especially in geriatric patients. Due to the great relevance, this study was aimed at describing the case of severe COVID-19 with development of multiple organ failure. Materials and methods. Available accompanying medical documentation (outpatient charts, medical history) was analyzed. Clinical and morphological analysis was carried out by providing description of macro- and micropreparations; histological methods (hematoxylin and eosin staining, Lee reaction) were used. Results. Female patient K.G., 69 years old, was hospitalized to the therapeutic department diagnosed with coronary heart disease. Acute coronary syndrome with ST segment elevation was made on 04/20/2020. A competing diagnosis: severe community-acquired bilateral multi-segmental pneumonia. The patient’s condition was aggravated wile applying therapy followed by biological death occurred. An autopsy revealed bilateral subtotal hemorrhagic pneumonia. Macroscopic lung examination demonstrated “lungs filled with red fluid”. In the brain — perivascular and pericellular edema, hyalinosis, blood stasis and sludge, marked dystrophic and necrotic neuronal changes. Cardiomyocyte fragmentation, areas of perivascular sclerosis with inflammatory infiltrates as well as erythrocytic sludge are found in the heart and blood vessels, respectively. A weak positive reaction according to Lee method was observed. Such clinical and morphological case demonstrates along with lung damage involvement of the heart resulting in acute coronary syndrome (morphologically manifested by ischemic myocardial dystrophy) and the brain. Thus, premorbid background in elderly patients results in developing acute pulmonary heart failure, pulmonary and cerebral edema.
oai:iimmun.ru:article/113
2022-04-12T21:18:55Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/113
2022-04-12T21:18:55Z
Russian Journal of Infection and Immunity
Vol 2, No 4 (2012); 747-752
THE SEASONAL INCREASE OF ENTEROVIRAL INFECTION INCIDENCE IN THE NOVGOROD OBLAST
Bichurina M.A.; ФБУН НИИ эпидемиологии и микробиологии имени Пастера, Санкт-Петербург
Pianykh V.A.; Управление Роспотребнадзора по Новгородской области, г. Великий Новгород
Novikova N.A.; ФБУН Нижегородский НИИ эпидемиологии и микробиологии им. акад. И.Н. Блохиной, г. Нижний Новгород
Leonova N.P.; Управление Роспотребнадзора по Новгородской области, г. Великий Новгород
Klevtsova G.A.; ФБУЗ Центр гигиены и эпидемиологии в Новгородской области, г. Великий Новгород
Romanenkova N.I.; ФБУН НИИ эпидемиологии и микробиологии имени Пастера, Санкт-Петербург
Ivanova T.G.; ФБУЗ Центр гигиены и эпидемиологии в Новгородской области, г. Великий Новгород
Golitsina L.N.; ФБУН Нижегородский НИИ эпидемиологии и микробиологии им. акад. И.Н. Блохиной, г. Нижний Новгород
Fomina L.B.; ФБУН Нижегородский НИИ эпидемиологии и микробиологии им. акад. И.Н. Блохиной, г. Нижний Новгород
Rozaeva N.R.; ФБУН НИИ эпидемиологии и микробиологии имени Пастера, Санкт-Петербург
Tseits O.E.; Управление Роспотребнадзора по Новгородской области, г. Великий Новгород
Lukovnikova L.B.; ФБУН Нижегородский НИИ эпидемиологии и микробиологии им. акад. И.Н. Блохиной, г. Нижний Новгород
Kanaeva O.I.; ФБУН НИИ эпидемиологии и микробиологии имени Пастера, Санкт-Петербург
Epifanova N.V.; ФБУН Нижегородский НИИ эпидемиологии и микробиологии им. акад. И.Н. Блохиной, г. Нижний Новгород
2014-07-04 00:00:00
Authors who publish with this journal agree to the following terms:
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Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/113
энтеровирусная инфекция; эпидемиологический надзор; вирусологический надзор; энтеровирусы
ru
Abstract. The analysis of epidemic process and definition of the etiology of the seasonal increase of serosal meningitis incidence in the Novgorod oblast in September-November, 2008 has been carried out. It was shown that in the specified period 73 persons had enteroviral meningitis. The proportion of children under 17 years old in the group of patients was 89.9%. The etiological agents of disease (enteroviruses ECHO 6 и ECHO 30) were detected by the virological and molecular-biological methods. It was established that two genetic variants of ECHO 6 and one variant of ECHO 30 co-circulated in the Novgorod oblast. The last virus variant was closely related to the strain isolated from enteroviral meningitis patients in Nizhniy Novgorod in 2007. The obtained data support the idea about necessity of systematic epidemiological and virological surveillance on enteroviral infection to get new information about circulation of non-poliomyelitis enteroviruses in the population and to define peculiarities of epidemic process development.
oai:iimmun.ru:article/403
2022-04-12T15:18:34Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/403
2022-04-12T15:18:34Z
Russian Journal of Infection and Immunity
Vol 6, No 2 (2016); 173-178
ANALYSIS OF TICKS OF IXODES PERSULCATUS И DERMACENTOR RETICULATUS SPECIES WITH TRANSMISSIBLE DISEASES IN KIROV REGION
Volkov S.A.; Vyatka State University
Bessolytsina E.A.; Vyatka State University
Stolbova F.A.; Vyatka State Agricultural Academy
Darmov I.V.; Vyatka State University
2016-06-25 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/403
клещевые трансмиссивные инфекции;клещевой энцефалит;болезнь Лайма;боррелиоз;анаплазмоз;эрлихиоз;бабезиоз;полимеразная цепная реакция
Беляева Т.А., Хмелина Н.А.
ru
Borreliosis, encephalitis, anaplasmosis, ehrlichiosis and babesiosis belongs to tick-borne transmissive diseases. These diseases are dangerous for human and animals as well. Moreover, some animals can have no clinical signs of these diseases. These diseases are widely spread across Russian Federation, although only encephalitis and borreliosis (Lyme disease) are being monitored nowadays. At the same time anaplasmosis, ehrlichiosis and babesiosis (pyroplasmosis) are not being monitored. Thus a goal of monitoring of these diseases appears. The main vector for these diseases are ticks. Ticks can carry and transmit causative agents of the diseases to domestic and wild animals. Thus a goal of monitoring transmissible diseases in different population of ticks gathered in our country appears. In this study PCR was chose. This method is perspective and is widely used to detect infectious diseases nowadays. Moreover this method allows getting results in quite short period of time. The goal of this work is to determine the presence of causative agents of tick-borne diseases in ticks of different species gathered in different areas of Kirov region in 2010–2015 with the help of PCR. Moreover the goal was to determine if there is a relation between a number of infected ticks and its species. To solve these goal a primers, PCR conditions, method of extraction of total nucleic acid from fixed and alive ticks were engineered. Method of extraction of total nucleic acids allowed with the help of a reverse transcriptase to determine tick-borne encephalitis virus in samples. Analyzed ticks were gathered in Kirov region. It was determined that main vectors in these region are of an Ixodes persulcatus and a Dermacentor reticulatus species. It was prooved that the number of infected ticks can vary in time. It was also proved that there is no significant difference in the number of infected with TBEV and Lyme disease causative agents but there is a significant difference in the number of infected ticks with anaplasmosis, ehrlichiosis and babesiosis causative agents.
oai:iimmun.ru:article/1795
2023-11-19T09:12:09Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/1795
2023-11-19T09:12:09Z
Russian Journal of Infection and Immunity
Vol 12, No 2 (2022); 381-385
Impact of <i>Candida spp.</i> metabolites on human skin fibroblasts
https://iimmun.ru/iimm/article/download/1795/11644
https://iimmun.ru/iimm/article/download/1795/11645
https://iimmun.ru/iimm/article/download/1795/11646
https://iimmun.ru/iimm/article/download/1795/11647
https://iimmun.ru/iimm/article/download/1795/11648
https://iimmun.ru/iimm/article/download/1795/11649
https://iimmun.ru/iimm/article/download/1795/11650
https://iimmun.ru/iimm/article/download/1795/11651
https://iimmun.ru/iimm/article/download/1795/11652
https://iimmun.ru/iimm/article/download/1795/11653
https://iimmun.ru/iimm/article/download/1795/11654
https://iimmun.ru/iimm/article/download/1795/12581
Ignatova N.I.; Privolzhsky Research Medical University of the Ministry of Health of the Russian Federation
Zaslavskaya M.I.; Privolzhsky Research Medical University of the Ministry of Health of the Russian Federation
Alexandrova N.A.; Privolzhsky Research Medical University of the Ministry of Health of the Russian Federation
Orlova O.E.; City Clinical Hospital No. 67 named after L.A. Vorokhobov
Melnikov V.G.; Moscow Research Institute of Epidemiology and Microbiology named after G.N. Gabrichevsky of Rospotrebnadzor
2022-05-13 14:39:44
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1795
Candida spp.; метаболиты; ферментативная активность; дермальные фибробласты; биоцидность; цитопатическое действие
Array
ru
Micromycetes spp. have been increasingly involved in the etiology of infectious diseases guiding to consider them not as important as bacterial and viral pathogens. Nowadays, a lot of severe forms of candidiasis are caused by C. auris, C. albicans, whereas C. glabrata and C. krusei are of similar importance. Members of these species were selected to investigate related metabolite action on human skin fibroblasts. Candida spp. being continuously found on the epithelium and mucosal membranes resulting in to sustained interaction between microbiota and human cells. Potential to produce metabolites containing pathogenicity factors is one of the crucial events for transition to invasive candidiasis, wherein human epithelial cells build up the front line of defense barrier preventing Candida spp. invasion into deeper host tissues. The study was aimed at assessing data on metabolite effects derived from epidemiologically relevant Candida spp. on primary human skin fibroblast culture in vitro. In particular, there were analyzed Candida spp. metabolites acting on fibroblast monolayer integrity and viability in cell suspension. It was found that Candida spp. metabolites might directly cause fibroblast death so that biocidal activity was exhibited as a strain-specific feature. A direct biocidity against dermal cells was more typical for strains C. glabrata и C. krusei, less pronounced for C. albicans and very weak for C. auris. In addition, a mechanism for secretory product-related biocidal activity derived from various Candida spp. on dermal fibroblasts in vitro revealed that it resulted in fibroblasts death 1 hour after exposure that peaked at 3 hrs. Cell death was equally proceeded via apoptosis and necrosis. Of note, biocidal effect of fungal metabolites showed no correlation with Candida-related potential to cleave intercellular junctions. It was found that C. auris metabolites showing weak biocidity against some fibroblasts simultaneously resulted in more marked disruption of cell monolayer compared to other Candida spp. Perhaps, it is just a feature of C. auris that might account for its higher invasiveness potential allowing to destroy tight human tissues more effectively compared to other Candida spp.
oai:iimmun.ru:article/10360
2023-12-15T21:48:03Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/10360
2023-12-15T21:48:03Z
Russian Journal of Infection and Immunity
Vol 13, No 5 (2023); 972-978
Etiology of community-acquired pneumonia in children and adults based on 2015–2022 bacteriological and molecular genetic studies
https://iimmun.ru/iimm/article/download/10360/93542
https://iimmun.ru/iimm/article/download/10360/93543
https://iimmun.ru/iimm/article/download/10360/93544
https://iimmun.ru/iimm/article/download/10360/93545
https://iimmun.ru/iimm/article/download/10360/93546
https://iimmun.ru/iimm/article/download/10360/93547
https://iimmun.ru/iimm/article/download/10360/93548
https://iimmun.ru/iimm/article/download/10360/93549
https://iimmun.ru/iimm/article/download/10360/93550
https://iimmun.ru/iimm/article/download/10360/93551
https://iimmun.ru/iimm/article/download/10360/157959
Sergevnin V.I.; Perm State Medical University named after Academician E.A. Wagner
Rozhkova M.V.; Perm State Medical University named after Academician E.A. Wagner
Ovchinnikov K.V.; Perm Regional Center for Control and Prevention of AIDS and Infectious Diseases
Kuzovnikova E.Z.; Perm Center of Hygiene and Epidemiology in Perm Region of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
2023-11-30 23:31:31
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/10360
внебольничная пневмония; дети; взрослые; бактериологический метод; молекулярно-генетический метод; виды бактерий; респираторные вирусы
Array
ru
The data on etiology of community-acquired pneumonia (CAP) remain contradictory, which is often assessed only according to bacteriological studies coming to a conclusion about the low frequency of major main pathogen Streptococcus pneumoniaе isolation. At the same time, there are very few observations of parallel examination of patients with CAP simultaneously by bacteriological and molecular genetic methods. There are no observations on the etiology of CAP in the long-term dynamics. The aim of the work is to analyze the etiology of CAP in children and adults based on 2015–2022 bacteriological and molecular genetic studies. The etiology of pneumonia was assessed using the data on posterior pharyngeal wall washes from 418 children and 483 adults hospitalized with a primary diagnosis of CAP at two large Perm medical organizations in 2015–2022. The samples were examined by the bacteriological method and polymerase chain reaction using commercial test systems with the amplifier “CFX96” (Bio-Rad, USA). The results showed that S. pneumoniaе, despite implementing decreed vaccinations against pneumococcal infection, it remains the leading causative agent of CAP in both children and adults. The 2015–2022 frequency of pneumococcal discharge according to bacteriological studies was 9.7 per 100 examined, according to molecular genetic studies — 48.5 per 100 examined among children and adults in the study area. According to 2021–2022 vs 2015–2017 bacteriological studies, the frequency of Staphylococcus aureus excretion among children increased significantly, and S. pneumoniaе increased among adults. According to the results of molecular genetic studies among children and adults, no significant changes in the etiology of CAP were found. In 2021–2022, SARS-CoV-2-positive vs SARS-CoV-2-negative patients with CAP infected had higher rate of isolated S. aureus, Klebsiella pneumoniaе and Hemophilus influenzae, i.e., those pathogens whose number increased in the long-term follow-up observations.
oai:iimmun.ru:article/692
2022-04-12T15:47:21Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/692
2022-04-12T15:47:21Z
Russian Journal of Infection and Immunity
Vol 9, No 3-4 (2019); 600-606
Monitoring of cytogenetic instability by micronuclei assay of both immunocompetent and non-immunocompetent cells in tick-borne encephalitis patients depending on variants of glutathione-S-transferase genes in the genotype
https://iimmun.ru/iimm/article/download/692/3673
https://iimmun.ru/iimm/article/download/692/3674
https://iimmun.ru/iimm/article/download/692/3675
https://iimmun.ru/iimm/article/download/692/3676
https://iimmun.ru/iimm/article/download/692/3678
https://iimmun.ru/iimm/article/download/692/3680
Ilyinskikh N.N.; Siberian State Medical University, National Research Tomsk State University
Ilyinskikh E.N.; Siberian State Medical University, National Research Tomsk State University
Zamyatina E.V.; Siberian State Medical University
Lee S.V.; Siberian State Medical University
2019-11-15 19:17:55
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/692
клещевой энцефалит, микроядерный анализ, буккальный эпителий, цитокинез-блокированные лимфоциты, GSTM1, GSTT1
RFFI #16-40-700149
ru
Aim of this study was to study the dynamics of the frequency of cytokinesis-blocked T-lymphocytes with micronuclei in peripheral blood and the frequency of buccal micronucleated epithelium cells for a period of half a year in patients with acute tick-borne encephalitis, depending on burden of active and inactive variants of glutathione-S-transferase genes (GSTM1 and GSTT1) in the patient's genotype. We carried out micronucleus assay in immunocompetent and non-immunocompetent cells in 54 patients with acute tick-borne encephalitis and 35 healthy persons (control) residing in the Tomsk and Tyumen regions. To analyze the frequency of cytokinesis-blocked micronucleated T-lymphocytes was used venous peripheral blood as material for phytohemagglutinin-stimulated cultures, and to study the frequency of buccal micronucleated cells, samples of the buccal mucous membrane epithelial cells were obtained. To carried out both techniques of micronucleus assay, cytological preparations were prepared, which were stained using the Giemsa or Felgen methods. The material for the study was obtained repeatedly during admission of patients to treatment, and also after 1 week, 1, 3 and 6 months. Polymerase chain reaction was used to analyze the alleles of the GSTM1 and GSTT1 genes. As a result of this analysis was found a significant increase in the frequency of micronucleated cells in tick-borne encephalitis patients compared with the control group. In addition, the frequency of cytokinesis-blocked micronucleated T-lymphocytes was increased significantly higher than the one of micronucleated buccal cells. The most significant and prolonged increase in the frequency of micronucleated cells was associated with the mutant inactive variants of the genes GSTM1 (0/0) and GSTT1 (0/0). In the patients with burden the inactive forms of these genes, the cytogenetic instability of the cytokinesis-blocked blood T-lymphocytes could persist for up to six months. In case of buccal cells, the frequency of micronucleated cells was close to the one in the control group as early as 1-3 months after a course of treatment. Conclusion. It was found that the most increased and prolonged frequency of cytogenetically instable cells persisted in cytokinesis-blocked T-lymphocytes of peripheral blood of patients with tick-borne encephalitis who were carriers of the genotype with inactive variants of both GSTM1 (0/0) and GSTT1 (0/0 ) glutathione-S-transferase genes.
oai:iimmun.ru:article/1496
2022-04-12T15:55:10Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/1496
2022-04-12T15:55:10Z
Russian Journal of Infection and Immunity
Vol 11, No 1 (2021); 171-176
A herd immunity to measles and rubella viruses in the population of the Republic of Serbia
https://iimmun.ru/iimm/article/download/1496/8944
https://iimmun.ru/iimm/article/download/1496/8945
https://iimmun.ru/iimm/article/download/1496/8946
https://iimmun.ru/iimm/article/download/1496/8947
https://iimmun.ru/iimm/article/download/1496/8948
https://iimmun.ru/iimm/article/download/1496/8949
https://iimmun.ru/iimm/article/download/1496/8950
https://iimmun.ru/iimm/article/download/1496/8951
https://iimmun.ru/iimm/article/download/1496/8952
https://iimmun.ru/iimm/article/download/1496/10334
Bichurina M.A.; St. Petersburg Pasteur Institute
Filipovic-Vignjevic S.; Institute of Virology, Vaccines and Sera Torlak
Antipova A.Y.; St. Petersburg Pasteur Institute
Bancevic M.; Institute of Virology, Vaccines and Sera Torlak
Lavrentieva I.N.; St. Petersburg Pasteur Institute
2021-02-28 19:23:46
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1496
Республика Сербия; коллективный иммунитет; иммунизация; вирус; корь; краснуха
ru
According to the WHO Strategic Plan, measles should be eradicated in 2020 in the five WHO Regions including European Region. However, large measles outbreaks are being periodically registered in diverse European countries. In the Republic of Serbia (SRB), 5,076 measles cases were detected in 2018, among which 15 cases were fatal.Aim of the study was to examine herd immunity to measles and rubella viruses in the population of the Republic of Serbia.Materials and methods. Blood serum samples obtained in 2018 and 2019 from conditionally healthy residents of the Republic of Serbia were tested for the presence of IgG antibodies to measles and rubella viruses in five age groups: I — children from 2 to 6 years old, II — children from 8 to 14 years old, III — 15 to 24 years old, IV — 25 to 49 years old and V — over 50 years old. A total of 1000 samples were obtained, 200 sera in each group. Enzygnost® Anti-Measles virus/IgG and Enzygnost® Anti-Rubella virus/IgG ELISA test systems (Siemens Healthcare Diagnostics Products GmbH, Germany) were used according to the manufacturer's instructions.Results. Overall, around 23.0% and 33.7% of the surveyed persons had no or low level of anti-measles IgG antibody (≥ 275.0 — ≤ 1000.0 IU/1). In age group I, 60% children contained no or “low” anti-measles antibodies titer (29.5% and 30.5%, respectively). In addition, low antibody titer level was mainly detected in individuals from age group II and III (p < 0.05). A third of children under 8—14 contained high IgG-antibodies titer against measles (> 3000.0 IU/l) that might serve as an evidence that such subjects recently recovered after measles. Similar results were obtained for IgG antibodies to rubella in the same age groups.Discussion. The study results evidence about altered routine immunization against measles and rubella in children aged 12—15 months (first vaccination) and those at age of 6—7 years (revaccination) with MMR vaccine. The data obtained correlate with official data on coverage with measles and rubella vaccines in the Republic of Serbia.
oai:iimmun.ru:article/1956
2023-11-19T05:07:06Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/1956
2023-11-19T05:07:06Z
Russian Journal of Infection and Immunity
Vol 12, No 5 (2022); 971-975
Immune response evaluation in the guinea pigs after immunization with the experimental Puumala virus vaccine
https://iimmun.ru/iimm/article/download/1956/13416
https://iimmun.ru/iimm/article/download/1956/13417
https://iimmun.ru/iimm/article/download/1956/13418
https://iimmun.ru/iimm/article/download/1956/13419
https://iimmun.ru/iimm/article/download/1956/13420
https://iimmun.ru/iimm/article/download/1956/13421
https://iimmun.ru/iimm/article/download/1956/13423
https://iimmun.ru/iimm/article/download/1956/14064
https://iimmun.ru/iimm/article/download/1956/14065
https://iimmun.ru/iimm/article/download/1956/14067
https://iimmun.ru/iimm/article/download/1956/14197
https://iimmun.ru/iimm/article/download/1956/14198
https://iimmun.ru/iimm/article/download/1956/14201
https://iimmun.ru/iimm/article/download/1956/14202
https://iimmun.ru/iimm/article/download/1956/14255
https://iimmun.ru/iimm/article/download/1956/14875
Kurashova S.S.; Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of the Russian Academy of Sciences
Balovneva M.V.; Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of the Russian Academy of Sciences
Ishmukhametov A.A.; Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of the Russian Academy of Sciences; Sechenov First Moscow State Medical University (Sechenov University)
Teodorovich R.D.; Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of the Russian Academy of Sciences
Popova Y.V.; Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of the Russian Academy of Sciences
Tkachenko E.A.; Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of the Russian Academy of Sciences
Dzagurova T.K.; Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of the Russian Academy of Sciences
2022-11-16 17:28:11
Authors who publish with this journal agree to the following terms:
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Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1956
геморрагическая лихорадка с почечным синдромом; вирус Пуумала; хантавирусный вакцинный препарат; схема вакцинации; нейтрализующие антитела; иммунный ответ
Array
ru
In the Russian Federation, the vaccine against hemorrhagic fever with renal syndrome is at the stage of preclinical and clinical trials. The aim of the study was to analyze an effect of vaccine schedule on neutralizing antibodies (nAB) dynamics in guinea pig models applied with experimental Puumala virus based hantavirus vaccine (HV). Quantitative evaluation of neutralizing antibodies was presented as antibody titer geometric mean expressed in binary logarithms (log2) by the 50% reduction of focus-forming units (FRNT50) in Vero cell in the focus reduction neutralization test. The HV dual inoculation to guinea pigs was carried out in 14 day intervals, booster injection was applied on day 182 after the onset, in the thigh muscle tissue by using 0.3 ml undiluted (HV-u/d) and in the 1/10 dilution (HV-1/10). nAB titer on day 14 after the first HV-u/d and HV-1/10 injection was measured to be at titer of 5.5±0.3 and 4.8±0.3, respectively. After the second injection, the nAB peak was as high as 9±0.2 on day 42 after the first HV-u/f injection, and 6.5±0.2 on day 14 after the HV-1/10 injection. nAB decreased down to 6.2±0.3 and 5±0.3, respectively, on day 364 after the first injection. The booster HV-u/d and HV-1/10 injection induced increase in nAB up to 9.5±0.3 and 6.5±0.3, respectively. After the booster injection, it induced significantly higher nAB observed on day 238 after the first HV-u/d injection and delayed up to the 294 day for the HV-1/10. The results of the study indicated the early formation of the immune response, long-term nAB persistence and significantly enhanced immune response after the booster injection on day 182, which indicated a potential for the booster injection a year later. The immunological efficacy and protective activity of the vaccine schedule may be finally assessed according to the results of clinical trials.
oai:iimmun.ru:article/600
2022-04-12T15:21:14Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/600
2022-04-12T15:21:14Z
Russian Journal of Infection and Immunity
Vol 7, No 4 (2017); 413-418
K. OXYTOCA BACTERIOPHAGES ISOLATION METHODS IMPROVEMENT
Sadrtdinova G.R.; Ulyanovsk State Agrarian University named after P.A. Stolypin, Ulyanovsk region
2018-01-20 00:00:00
Authors who publish with this journal agree to the following terms:
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Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/600
бактерии; бактериофаги; выделение; метод; морфология; литическая активность
ru
The article presents the results of a study related to increasing the efficiency of phage isolation of bacteria of the species K. oxytoca, by developing the optimal composition of the medium used in the work. In scientific research, in almost all methods associated with the isolation of bacteriophages, meat-peptone broth and meat-peptone agar are used as the nutrient basis. The peculiarities of growth and cultivation of microorganisms create certain difficulties for the isolation of phages active against bacteria of the species K. oxytoca. The selection of components and the creation of an environment that would ensure the optimal growth of both the bacterial culture and the reproduction of the virus makes it possible to facilitate the isolation of bacteriophages. The number of bacterial strains used in the work was 7. All strains of cultures were obtained from the Museum of the Department of Microbiology, Virology, Epizootology and Veterinary and Sanitary Expertise of the Federal State Budget Educational Institution of Higher Education “Ulyanovsk State Agrarian University named after P.A. Stolypin”. The studies included 2 main stages. The first stage consisted in isolation of bacteriophages by the method of isolation from the external environment by the method of Adelson L.I., Lyashenko E.A. The material for the studies were samples: soil, sewage sample, fecal samples (2). Only 4 samples. According to the chosen method, the sowing of the putative phagolysate was carried out on meat-peptone agar (1.5%) and the agar for isolating bacteriophages (Aph) (1.5%). A positive result was the presence on the environment of negative colonies, clearly visible on the matt background of deep growth of bacteria. A negative result is a continuous growth (“lawn”) of bacterial culture. As a control, the culture of the microorganism studied was used for the media. In the course of the conducted studies for the first stage, 2 bacteriophages were isolated, active against the desired species of microorganism. The second stage consisted in a comparative analysis of the indices of cultivation of the isolated phages on standard meat-peptone agar and the proposed medium for isolation of bacteriophages. The main biological properties of the phages studied were also determined: morphology of negative colonies, lytic activity, specificity. The negative colonies formed on the compared media were classified as one type. The lytic activity of the studied phages on meat-peptone agar and Aph medium was the same. According to Grazia 2 × 107 BOE/ml for bacteriophage KO-3 UGSHA, (3–4) × 108BOE/ml for bacteriophage KO-8724 UGSHA. The isolated bacteriophages showed no lytic activity against strains of heterogeneous crops (Escherichia spp., Citrobacter spp., Serratia spp., Hafnia spp., Yersinia spp.).
oai:iimmun.ru:article/1173
2022-04-12T15:51:16Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/1173
2022-04-12T15:51:16Z
Russian Journal of Infection and Immunity
Vol 10, No 2 (2020); 375-380
Pattern of resilient age-related measles immunity
https://iimmun.ru/iimm/article/download/1173/5778
https://iimmun.ru/iimm/article/download/1173/5779
https://iimmun.ru/iimm/article/download/1173/5780
https://iimmun.ru/iimm/article/download/1173/5781
https://iimmun.ru/iimm/article/download/1173/5782
https://iimmun.ru/iimm/article/download/1173/5783
https://iimmun.ru/iimm/article/download/1173/5784
https://iimmun.ru/iimm/article/download/1173/5785
https://iimmun.ru/iimm/article/download/1173/5786
https://iimmun.ru/iimm/article/download/1173/5787
https://iimmun.ru/iimm/article/download/1173/7920
Sonis A.G.; Samara State Medical University of MH RF; Clinics of Samara State Medical University
Gusyakova O.A.; Samara State Medical University of MH RF; Clinics of Samara State Medical University
Gilmiyarova F.N.; Samara State Medical University of MH RF
Ereshchenko A.A.; Samara State Medical University of MH RF; Clinics of Samara State Medical University
Ignatova N.K.; Samara State Medical University of MH RF; Clinics of Samara State Medical University
Kuzmicheva V.I.; Samara State Medical University of MH RF
Borodina I.A.; Samara State Medical University of MH RF
Nenjajkin S.S.; Clinics of Samara State Medical University
2020-03-03 13:43:36
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1173
корь; иммунитет; иммуноглобулин G; вакцинация; ревакцинация; медицинские работники
ru
Epidemiological situation describing global measles spread is ambiguous. Along with countries succeeded in measles eradication, there are those wherein measles rate remains at quite high level. Because measles is a vaccine-preventable infection, it may then be eradicated solely by ensuring sufficient population coverage with preventive vaccination. The aim of our study was to assess level of measles immunity in medical workers at the Clinics of Samara State Medical University as well as the Samara State Medical University. There were enrolled 1503 subjects (aged 18–79 years), among which all individuals under 55 (77.58%) but not older counterparts provided with medical record on previous measles vaccination or measles infection. Level of serum measles virus-specific IgG antibodies was measured by using ELISA (VektoKor-IgG, JSC Vector-Best, Novosibirsk), with mean concentration ranging in general population within 1.02±0.02 IU/ ml. Positive results were observed in 72.52% of the examined individuals. Average vs. high measles virus-specific IgG level was detected in 52.90% (mean age — 41.4±0.5 years) and 19.62% (mean age — 54.2±0.72 years) of individuals, whereas at level below threshold — in 27.48% of subjects (mean age — 33.25±0.53 years). Thus, in 34.16% of the surveyed vaccinated individuals mostly presented by young subjects contained anti-measles virus-specific antibodies below protective level. Older age groups were shown to increase in average IgG amount with age. Interestingly, age-related measles immunity pattern was observed: percentage of subjects with high vs. low measles virus-specific IgG level increases and decreases, respectively. Taking into consideration a large percentage of subjects previously vaccinated against measles among carriers of low measles immunity, it may be concluded that measles virus-specific IgG antibody level must be monitored in young adulthood to decide of whether subsequent revaccination is necessary.
oai:iimmun.ru:article/71
2022-04-12T21:20:02Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/71
2022-04-12T21:20:02Z
Russian Journal of Infection and Immunity
Vol 1, No 4 (2011); 367-372
CYTOKINES AS MARKERS OF THE INFILTRATIVE PULMONARY TUBERCULOSIS DEVELOPMENT
Mezentseva M.V.; ФГБУ НИИ эпидемиологии и микробиологии им. Н.Ф. Гамалеи Минздравсоцразвития России, Москва
Stakhanov V.A.; ГОУ ВПО Российский государственный медицинский университет им. Н.И. Пирогова Росздрава, Москва
Zakharova M.V.; Противотуберкулезный клинический диспансер № 4, Москва
Zotova I.F.; ФГБУ НИИ эпидемиологии и микробиологии им. Н.Ф. Гамалеи Минздравсоцразвития России, Москва
Tregubova M.I.; ФГБУ НИИ эпидемиологии и микробиологии им. Н.Ф. Гамалеи Минздравсоцразвития России, Москва
Shapoval I.M.; ФГБУ НИИ эпидемиологии и микробиологии им. Н.Ф. Гамалеи Минздравсоцразвития России, Москва
2014-07-01 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/71
впервые выявленный инфильтративный туберкулез легких; иммунитет; цитокины; интерлейкины; интерферон
ru
Abstract. Many parts of immune system are involved in interaction of M. tuberculosis and human organism. There are some publications concerning significance of interferon and other cytokines in lung tuberculosis. Our research group investigated condition of interferon and cytokines systems in patients with firstly detected infiltrative lung tuberculosis. The results of this study have shown direct links between clinical and immunological efficacy of the complex treatment of such patients. The active expression of IL-6 gene correlates with slow dynamics of lung infiltrate resolution and long saving of patient’s complaint for respiratory tract symptoms during the treatment. The expression of IL-12 gene linked to small volume of infiltrates in lung tissue before treatment whereas expression of IL-1β gene correlates with decrease of intoxication time and respiratory symptoms in patients during therapy. The knowledge about violation in cytokine regulation parts is absolutely necessary to determine dynamics of diseases development and treatment efficacy as well as to use immunotherapy.
oai:iimmun.ru:article/333
2023-08-06T17:27:12Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/333
2023-08-06T17:27:12Z
Russian Journal of Infection and Immunity
Vol 5, No 3 (2015); 279-284
LABORATORY TESTING RESULTS ANALYSIS OF GIARDIASIS PREVALENCE AMONG DIFFERENT POPULATION GROUPS IN THE NENETS AUTONOMOUS DISTRICT
Bobyreva N.S.; Northern State Medical University, Institute of Arctic Medicine, Arkhangelsk, Russian Federation
Degteva G.N.; Northern State Medical University, Institute of Arctic Medicine, Arkhangelsk, Russian Federation
2015-11-30 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/333
коренное население;ненцы;кочевой образ жизни;оседлый образ жизни;лямблиоз;протозоозы;труднодоступные регионы Арктической зоны РФ
ru
The Nenets Autonomous District (NAD) refers to the territories of the Russia’s Far North. The country is the only region of Russia, where there are still no roads. The extreme weather conditions, as well as residential patterns in the NAD reduce the availability of specialized medical care, as indigenous people and other nationalities living in the district, which requires the use of health care management specific forms. The article presents data on giardiasis studies amongst different categories of the NAD population, obtained as a result of the population surveys in remote regions of the Russian Federation Arctic zone. Samples studied were serum and faecal emulsion. The study used the following laboratory methods: for serum — Giardia lamblia antibody screening method using ELISA kit, emulsion stool — native smear microscopy analysis, the method of enrichment using disposable concentrators «Parasep» immunological diagnostic methods based on the detection of specific lamblia antigen in faeces using ELISA kits. For statistics was used descriptive statistics analysis (mean values, the percentage error of the mean, the construction of the trend line) with program SPSS 20.00, Excel 2010, the method of analysis of official statistical reporting hospitals survey on giardiasis, data of Rospotrebnadzor (Russian Sanitary service) for NAD and data from own research from 2002 to 2013. Just in the process of research carried out research on the 10 356 giardiasis, registered diseases by 3470 cases of giardiasis, accounting for 99.14% of the total number of protozoonosises registered and 37.4% of all registered in the NAD parasitosis. The comparison was made among the population of the county towns, home to both the indigenous people, nomadic, and representatives of other ethnic groups, sedentary with urban population of the city of Naryan-Mar. The article analyzes the incidence rates for giardiasis according to district official statistical reporting compared with the incidence in the Russian Federation for the same period of time. Statistical analysis was performed using the software package Excel 2010. The study found an association of giardiasis prevalence in the population of the NAD on criteria such as location, gender, occupation and age structure examined.
oai:iimmun.ru:article/1654
2023-11-16T17:08:15Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/1654
2023-11-16T17:08:15Z
Russian Journal of Infection and Immunity
Vol 12, No 1 (2022); 165-171
Changes in vascular endothelial growth factor level in <i>Helicobacter pylori</i>-associated gastroduodenal diseases
https://iimmun.ru/iimm/article/download/1654/10137
https://iimmun.ru/iimm/article/download/1654/10138
https://iimmun.ru/iimm/article/download/1654/10139
https://iimmun.ru/iimm/article/download/1654/10140
https://iimmun.ru/iimm/article/download/1654/10141
https://iimmun.ru/iimm/article/download/1654/10142
https://iimmun.ru/iimm/article/download/1654/10143
https://iimmun.ru/iimm/article/download/1654/10144
https://iimmun.ru/iimm/article/download/1654/10145
https://iimmun.ru/iimm/article/download/1654/10146
https://iimmun.ru/iimm/article/download/1654/10147
https://iimmun.ru/iimm/article/download/1654/10148
https://iimmun.ru/iimm/article/download/1654/10149
https://iimmun.ru/iimm/article/download/1654/12149
Matveeva L.V.; National Research Mordovia State University
Kapkaeva R.H.; National Research Mordovia State University
Chudaikin A.N.; National Research Mordovia State University
Soldatova A.A.; National Research Mordovia State University
Mosina L.M.; National Research Mordovia State University
Kostina Y.A.; National Research Mordovia State University
Solodovnikova G.A.; National Research Mordovia State University
2021-12-03 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1654
васкулоэндотелиальный фактор роста; Helicobacter pylori; пепсиноген; хронический гастрит; язвенная болезнь желудка; язвенная болезнь двенадцатиперстной кишки; корреляция; прогноз
ru
A populational infection with Helicobacter (H.) pylori poses a global problem. Mucosal colonization of H. pylori in the gastroduodenal area can initiate development multiple diseases with hyper- or hypoplasia of mucosal epithelial cells secreting vascular endothelial growth factor (VEGF). The aim of the study was to assess VEGF serum level, its diagnostic and prognostic value in diseases affecting the gastroduodenal area. Materials and methods. 180 patients with exacerbated chronic gastritis, gastric ulcer, duodenal ulcer as well as 30 healthy volunteers were examined after providing an informed consent. Patients were divided into groups depending on the degree of mucous contamination with H. pylori. In the subjects examined during esophagogastroduodenoscopy, a biological material was collected during targeted biopsy for microscopic and histological studies. Blood samples for immunological examination were obtained in the morning on an empty stomach from the ulnar vein in the volume of 5 ml, and the serum was isolated by centrifugation. The level of VEGF, pepsinogens, and titer of total antibodies against the H. pylori cytotoxin-associated protein were determined in the blood serum from the subjects by using the enzyme immunoassay method. The long-term prognosis was analyzed for up to 2 years. The data obtained were processed statistically. Results. Patients were found to have excessive serum VEGF levels in healthy volunteers. For gastric ulcer associated with H. pylori, 80% of cases had increased discriminatory VEGF level. In patients, direct relationships between the serum VEGF level and degree, stage of gastritis, the degree of contamination with H. pylori, the serum pepsinogens level were uncovered. Regression analysis found that patients with diseases targeting gastroduodenal area had serum VEGF level equal to or greater than 231 pg/ml in 60% of cases that correctly predicted an increase in mucosal atrophy. If the amount of VEGF ≥ 373 pg/ml in 91.5% of cases, then ulceration of gastric epithelium developed, whereas for ≥ 396 pg/ml level it was observed in 89% cases with ulceration of the intestinal epithelium. The probability of gastroduodenal bleeding at a serum VEGF level of 408 pg/ml or higher was predicted correctly in 96% of cases. Conclusion. More than 54% of patients with H. pylori-associated chronic gastritis, peptic ulcer disease had level of VEGF significantly exceeding magnitude found in healthy volunteers and the discriminatory level reflects the morphofunctional state of the stomach and duodenum. Assessing serum VEGF level in gastroduodenal diseases can be recommended for predicting development of atrophy, ulceration of the gastric and intestinal epithelium, and gastroduodenal bleeding.
oai:iimmun.ru:article/3667
2023-08-06T17:51:49Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/3667
2023-08-06T17:51:49Z
Russian Journal of Infection and Immunity
Vol 13, No 2 (2023); 383-390
A combination preparation based on antibiotic, cluster silver and bacteriophage for treatment of infectious diseases caused by <i>Staphylococcus aureus</i> and <i>S. aureus</i> MRSA
https://iimmun.ru/iimm/article/download/3667/39753
https://iimmun.ru/iimm/article/download/3667/39754
https://iimmun.ru/iimm/article/download/3667/39755
https://iimmun.ru/iimm/article/download/3667/39756
https://iimmun.ru/iimm/article/download/3667/39757
https://iimmun.ru/iimm/article/download/3667/39759
https://iimmun.ru/iimm/article/download/3667/39760
https://iimmun.ru/iimm/article/download/3667/52920
https://iimmun.ru/iimm/article/download/3667/52921
https://iimmun.ru/iimm/article/download/3667/52922
https://iimmun.ru/iimm/article/download/3667/52923
https://iimmun.ru/iimm/article/download/3667/73894
https://iimmun.ru/iimm/article/download/3667/109552
https://iimmun.ru/iimm/article/download/3667/109554
https://iimmun.ru/iimm/article/download/3667/109555
Pugachev V.G.; State Research Center of Virology and Biotechnology “Vector”, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
Totmenina O.D.; State Research Center of Virology and Biotechnology “Vector”, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
2023-04-24 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/3667
Staphylococcus aureus; бактериофаг; антибиотики; кластерное серебро; антибиотикорезистентность; фаговая терапия
Array
4/19
ru
The problems of bacterial infections in medicine and veterinary medicine require careful study and rapid solution. Due to continuous and, in some cases, irrational use of antibiotics, the efficiency of their effect on host has been noticeably decreasing; moreover, resistance to antibacterial drugs is steadily growing, antibiotic-resistant strains emerge, which are not amenable to conventional medical treatment. The unprecedented rise of pathogenic bacteria resistance to antibiotics requires generation of new drugs to combat them. One of the ways to increase the effectiveness of antibiotic therapy is to use combination drugs. Combination dosage forms provide an increased therapeutic effect and should not be toxic to the body. To overcome the microbial resistance reducing host burden of antibiotics, we proposed a combination preparation based on antibiotic, cluster silver and specific bacteriophage for treatment of infectious diseases caused by S. aureus, including MRSA strains. Each component has already proven in the treatment of pathogen-caused infectious diseases. But while using this combination agent, it became possible to reduce the amount of antibiotic and get rid of antibiotic-resistant and phage-resistant forms of bacteria. The study showed the effectiveness of the combination preparation on S. aureus MRSA bacteria, while reducing the amount of antibiotics in proposed composition by 2–4 times compared to use of antibiotic alone. The efficacy of the preparation containing was as follows: 10 mg/ml gentamicin, 7 mg/ml cluster silver and 106 BOV/ml bacteriophage, on S. aureus MRSA bacteria in suspension is comparable to the effectiveness of gentamicin with a concentration of 40 mg/ml. For S. aureus 209 and S. aureus ssp. bacteria with reduced resistance to antibiotics, the use of two-component compositions (antibiotic + cluster silver; antibiotic + bacteriophage) also allowed to reduce the antibiotic concentration by 2–3 times. It should be noted that with a constant amount of antibiotic, increasing the amount of cluster silver by 4–5 times, there is a rise in bactericidal properties of the resulting preparations. Repeated introduction of drug dose allows to achieve a marked decrease in level of pathogenic microorganisms in the suspension studied. This drug has no limitations depending on the state and degree of microbial resistance.
oai:iimmun.ru:article/701
2022-04-12T20:18:00Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/701
2022-04-12T20:18:00Z
Russian Journal of Infection and Immunity
Vol 9, No 2 (2019); 369-374
Inflammation induced by different plasmid types of russian <i>Yersinia pseudotuberculosis</i> strains
Somova L.M.; Somov Research Institute of Epidemiology and Microbiology
Shubin F.N.; Somov Research Institute of Epidemiology and Microbiology
Drobot E.I.; Somov Research Institute of Epidemiology and Microbiology
Lyapun I.N.; Somov Research Institute of Epidemiology and Microbiology
Plekhova N.G.; Somov Research Institute of Epidemiology and Microbiology;
Pacific State Medical University
2019-05-13 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/701
псевдотуберкулез; дальневосточная скарлатиноподобная лихорадка; Yersinia pseudotuberculosis; плазмидные типы; воспаление; клетки врожденного иммунитета; иммуносупрессия
Array
ru
In the 2000s, a scientific interest to the Far Eastern scarlet-like fever (FESLF) mainly recorded In Russia and Japanwas remarkably increased. Such clinical and epidemic manifestation of human pseudotuberculosis is related to a certain bacterial clonal lineage characterized by a specific plasmid profile (pVM82, pYV48), sequence type (2ST) as well as the yadA gene allele (1st allele). In our study we examined features of inflammatory changes characterizing plasmidassociated pathogenicity of the FESLF pathogen. In addition, organ histopathology in experimental animals infected intraperitoneally with Y. pseudotuberculosis strains of the four plasmid types 48+:82+; 48+:82-; 48-:82+; 48-:82-; and 48-:82- was investigated. It was found that the mortality rate in animals infected with Y. pseudotuberculosis H-5015 strain (82+:48+) bearing two plasmids with a molecular weight of 82 and 48 MDa was 40%. A picture of diffuse pneumonia with moderate inflammatory infiltration in pulmonary tissue and changes in lymphoid organs characterizing immunodeficiency we observed as early as 3 days postinfection (p.i.). On the contrary, animals infected with Y. pseudotuberculosis H-5015 strain (82+:48–) bearing a single plasmid 82 MDa pVM, mortality rate was 30%. A vascular reaction in the lungs and liver as well as deteriorated vascular destructive changes were revealed starting from day 3 and on day 5–7 days p.i., respectively, which was paralleled with perivascular infiltration mainly by mononuclear cells and focal pneumonia as well as a reaction of bronchialassociated lymphoid tissue and minimal organ destructive changes. On day 7 p.i., signs of granulomatous inflammation in the liver in a form of small mononuclear cell clusters and perivascular compact infiltrates were found. At all time points, lymphoid organ hyperplasia was noted. In case the infection caused by H-5013 strain (48+), the mortality rate was 80%. Inflammatory changes with dominant mononuclear cells were detected as early as 1 day p.i. associated with a picture of large focal bronchopneumonia, more pronounced in the non-survivor animals, also demonstrating signs of severe immunosuppression in the lymphoid organs. Starting from day 3 p.i., lymphoid hypoplasia in the spleen and lymph nodes was detected in all infected animals paralleled with pathogen-associated tissue damage in various organs. Animals infected with the plasmid-free H-5013 strain 48-) resulted in 25% mortality rate. Moreover, starting from day 3 p.i., a slight mononuclear inflammatory infiltration in the lungs and liver, a moderate giant cell reaction in the splenic pulp, and loose perinodal inflammatory infiltration in the lymph nodes were observed. Thus, while modeling infection caused by different plasmid types of Y. pseudotuberculosis, the data on differences in histopathology of changes in diverse organs regarding dynamics and generalization of the inflammatory response, as well as the severity of pathogen-associated damage in the lymphoid tissue were obtained. In case Y. pseudotuberculosis strains contained pVM82 plasmid with or without virulence plasmid pYV vs. single pYV-positive strains, an area of the inflammatory response as well as severity of immunosuppression manifested by splenic and lymph node delymphatization were decreased. It allowed to suggest that pVM82 plasmid found In Russia-originating Y. pseudotuberculosis strains might be implicated in limiting intensity of inflammatory response, bacterial dissemination and severity of lymphoid organ damage.
oai:iimmun.ru:article/1349
2022-04-12T15:54:55Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/1349
2022-04-12T15:54:55Z
Russian Journal of Infection and Immunity
Vol 11, No 1 (2021); 157-164
Altered interferon defense in children with dynamically changed infectious mononucleosis
https://iimmun.ru/iimm/article/download/1349/7383
https://iimmun.ru/iimm/article/download/1349/7384
https://iimmun.ru/iimm/article/download/1349/7385
https://iimmun.ru/iimm/article/download/1349/7386
https://iimmun.ru/iimm/article/download/1349/7387
https://iimmun.ru/iimm/article/download/1349/7388
https://iimmun.ru/iimm/article/download/1349/7389
https://iimmun.ru/iimm/article/download/1349/7390
https://iimmun.ru/iimm/article/download/1349/7523
https://iimmun.ru/iimm/article/download/1349/8874
https://iimmun.ru/iimm/article/download/1349/8875
https://iimmun.ru/iimm/article/download/1349/8876
https://iimmun.ru/iimm/article/download/1349/8877
Kukushkina E.A.; G.N. Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology
Koteleva S.I.; G.N. Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology
Blyakher M.S.; G.N. Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology
Fedorova I.M.; G.N. Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology
Ramazanova Z.K.; G.N. Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology
Zvereva N.N.; Pirogov Russian National Research Medical University
Novosad E.V.; Pirogov Russian National Research Medical University
Samkov A.A.; Infectious Hospital No. 1 Moscow Department of Healthcare
Bazarova M.V.; Infectious Hospital No. 1 Moscow Department of Healthcare
2021-02-28 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1349
инфекционный мононуклеоз; ВЭБ-инфекция у детей; IFNa; IFNy; интерфероновый статус; интерферонотерапия
ru
The state of the interferon system in 38 children with acute infectious mononucleosis (IM) caused by the Epstein—Barr virus was analyzed. Interferon status was examined in accordance with F.I. Ershov method based on assessing related biological activity by measuring interferon level in the blood serum or produced by blood cells. The aim of the study was to gain scientifically justified data for use of interferon preparations or interferonogens in IM combination therapy. For this, interferon status in children with acute IM was compared with that one not only in control group (30 healthy children, aged 3—6 and 20 children, aged 7—14 years, examined earlier to create an intra-laboratory interferon normal range), but also in children with lacunar angina or acute respiratory viral infection, hospitalized in the same department of the clinic and comparable with the main group in severity of the condition. In addition, we assessed changes in IFN-status in IM patients receiving no interferon preparations, one month after the disease onset. The study showed that patients with moderate acute IM were featured with decreased potential of blood leukocytes to virus-induced IFNa and mitogen-induced IFNy production observed with almost similar or some lower rate as in the control group of children hospitalized with angina or acute respiratory viral infection. Peripheral blood cells from moderate acute IM patients in the 3-6-year age group were found to produce virtually unaltered interferon level, whereas almost sole IFN-alpha production was affected in 7-14-year-old patients. Moreover, in 7-14-year old patients the level 1 and level 2 of IFNa deficiency was observed in 38% and 6% of cases, respectively. It is likely it was just this patient group requiring administration of any IFNa replacement therapy. As few as 12 children were re-examined after discharge from the clinic. Initially, prevalence and severity level of impaired interferon production in this subgroup did not differ from that one for total patient sample, whereas 1 month later a host potential to produce both IFNa and IFNy even without therapy acting on interferon system was noted to be moderately augmented.
oai:ojs.iimmun.ru:article/260
2016-02-16T11:02:38Z
iimm:SHORT
oai:iimmun.ru:article/150
2022-04-12T21:20:53Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/150
2022-04-12T21:20:53Z
Russian Journal of Infection and Immunity
Vol 3, No 4 (2013); 376-378
THE STUDY OF VIRUSES REPRODUCTION IN CELL CULTURES BY THE METHOD OF MATHEMATICAL MODELING
Kontarov N.A.; Research Institute of Vaccines and Sera of I.I. Mechnikov RAMS, Moscow
Grishunina S.A.; Lomonosov Moscow State University, Moscow
Balaev N.V.; Research Institute of Vaccines and Sera of I.I. Mechnikov RAMS, Moscow
Yuminova N.V.; Research Institute of Vaccines and Sera of I.I. Mechnikov RAMS, Moscow
Zverev V.V.; Research Institute of Vaccines and Sera of I.I. Mechnikov RAMS, Moscow
2014-07-09 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/150
размножение вируса; культура клеток; математическая модель Марчука; устойчивый узел
ru
Abstract. In the study the mathematical analysis of viruses reproduction in cell culture using the Marchuk’ mathematical model to predict reproduction of virus in one or another cell cultures has been conducted. The obtained theoretical results are corresponded to the experimental data on reproduction of rubella virus in cell cultures RK-13 and BHK-21. The sum of theoretical and experimental results can be used to select the optimal cell cultures for virus cumulation.
oai:iimmun.ru:article/1836
2023-11-17T07:51:15Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/1836
2023-11-17T07:51:15Z
Russian Journal of Infection and Immunity
Vol 12, No 3 (2022); 580-584
Estimating allergic sensitization to some mold fungi
https://iimmun.ru/iimm/article/download/1836/12125
https://iimmun.ru/iimm/article/download/1836/12126
https://iimmun.ru/iimm/article/download/1836/12127
https://iimmun.ru/iimm/article/download/1836/12128
https://iimmun.ru/iimm/article/download/1836/12129
https://iimmun.ru/iimm/article/download/1836/12130
https://iimmun.ru/iimm/article/download/1836/12131
https://iimmun.ru/iimm/article/download/1836/12132
https://iimmun.ru/iimm/article/download/1836/13093
https://iimmun.ru/iimm/article/download/1836/13941
https://iimmun.ru/iimm/article/download/1836/13942
https://iimmun.ru/iimm/article/download/1836/13943
Metelskaya V.А.; G.N. Gabrichevsky Scientific Research Institute for Epidemiology and Microbiology
Matveevskaya N.S.; G.N. Gabrichevsky Scientific Research Institute for Epidemiology and Microbiology
Grechishnikova O.G.; G.N. Gabrichevsky Scientific Research Institute for Epidemiology and Microbiology
Safronova A.V.; G.N. Gabrichevsky Scientific Research Institute for Epidemiology and Microbiology
2022-07-04 15:06:41
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1836
специфические IgE-антитела; распространенность; иммуноблот; плесневые грибы; атопическая сенсибилизация; аллергическая реакция
Array
ru
The assessment of allergic sensitization of 894 patients (478 and 416 of them examined in 2018 and 2019, respectively) who were examined at the consultative and Diagnostic center of G.N. Gabrichevsky Scientific Research Institute for Epidemiology and Microbiology, Moscow, for some mold fungi was carried out by measuring serum specific IgE antibody level by using a highly specific and highly sensitive immunoblot method (RIDA AllergyScreen test systems, R-Biopharm AG, Germany). The prevalence of patients with elevated levels of specific IgE antibodies to mold fungi Alternaria alternata, Aspergillus fumigatus, Cladosporium herbarum and Penicillium notatum was found. All the examined patients at each time point was divided into two age groups: group 1 — children aged 1 to 17 years, 548 children within the two-year period (303 of them girls and 245 boys), group 2 — adults aged at least 18 years old, 346 subjects within the same period (231 of them women and 115 men). Results. In 2018, out of 478 serum samples, atopic sensitization to mold fungi was detected in 25.3% (n = 121) of the examined subjects, of which 17.9% (n = 86) and 7.4% (n = 35) was found in group 1 and group 2, respectively. In 2019, out of 416 serum samples, atopic sensitization to mold fungi was detected in 20.7% (n = 86) of the examined subjects, of which 13.5% (n = 56) and 7.2% (n = 30) was found in group 1 and group 2, respectively. In 2018, out of 121 positive samples, specific IgE antibodies to various mold fungi were slightly more often detected in females — 53.7% (n = 65), of which 75.3% (n = 49) and 24.7% (n = 16) were girls and women, respectively. In contrast, in 2019, out of 86 positive samples, specific IgE antibodies to various mold fungi were slightly more often detected in males reaching 51.2% (n = 44), of which 79.5% (n = 35) and 20.5% (n = 9) were boys and men, respectively. Atopic sensitization to the mold fungus Alternaria alternata was most often detected: during the study period, out of 207 positive samples, it was found in 186 samples (89.9%). Serum IgE antibodies to mold fungi are more often detected in children under 17, in sex-independent manner.
oai:iimmun.ru:article/520
2022-04-12T15:20:18Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/520
2022-04-12T15:20:18Z
Russian Journal of Infection and Immunity
Vol 7, No 2 (2017); 203-208
THE ANTIGEN-SPECIFIC CELL IN VITRO TESTS FOR POST-VACCINATION ANTIPLAGUE IMMUNITY FORMATION
Kulichenko A.N.; Stavropol Plague Control Research Institute
Abzaeva N.V.; Stavropol Plague Control Research Institute
Gostischeva S.E.; Stavropol Plague Control Research Institute
Rakitina E.L.; Stavropol Plague Control Research Institute
Ponomarenko D.G.; Stavropol Plague Control Research Institute
Kostuchenko M.V.; Stavropol Plague Control Research Institute
2017-06-19 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/520
специфическая профилактика чумы;клеточный иммунитет;оценка эффективности вакцинации;маркеры активации лимфоцитов;антигены Yersinia pestis;коэффициент стимуляции
ru
The possibility of post-vaccination anti-plague immunity evaluation was researched using antigen-stimulated cells tests in vitro and cytometry analysis. The object of study — the blood samples of 17 people immunised by the live plague vaccine (Yersinia pestis EV) epicutaneously. Blood taking was carried out before vaccination and after immunisation on 7 and on 21 days, in 3 and in 6 months. Intensity antigen reactivity of lymphocytes was detected by cell tests in vitro, analysing markers of early (CD45+CD3+CD25+) and late (CD45+CD3+HLA-DR+) lymphocyte activation using flow cytometry. The complex of water-soluble Y. pestis antigens and allergen — pestin PP was tested as antigen. The high stimulating potential was defined of the water-soluble antigens Y. pestis complex. It is shown that coefficient of stimulation of relative level T- lymphocytes which express receptors for IL-2 was positive for all observation times after immunisation. The coefficient of stimulation had maximum values at 21 days (56.37%) and at 3 (47.41%) months. In identifying HLADR-positive lymphocytes before vaccination, the negative coefficient of stimulation was indicated on 7 and 21 days and the positive coefficient of stimulation was indicated at 3 and at 6 months. Analysis of intensity expression of early and late lymphocyte activation markers dynamics showed the possibility and prospect of application of cellular in vitro tests for the laboratory evaluation of specific reactivity of cellular immunity in both the early (7 days) and late (6 months) periods after vaccination. The results can be the basis for developing a new algorithm for assessment of immunological effectiveness of vaccination people against plague. It is the algorithm based on the identification of lymphocyte activation markers by antigen stimulation in conditions in vitro.
oai:iimmun.ru:article/1155
2023-08-06T17:12:06Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/1155
2023-08-06T17:12:06Z
Russian Journal of Infection and Immunity
Vol 9, No 5-6 (2019); 805-810
State of rat colon microbiocenosis in chronic restraint stress treated with Selank
https://iimmun.ru/iimm/article/download/1155/5452
https://iimmun.ru/iimm/article/download/1155/5453
https://iimmun.ru/iimm/article/download/1155/5454
https://iimmun.ru/iimm/article/download/1155/5455
https://iimmun.ru/iimm/article/download/1155/5456
https://iimmun.ru/iimm/article/download/1155/5457
https://iimmun.ru/iimm/article/download/1155/5458
https://iimmun.ru/iimm/article/download/1155/5459
https://iimmun.ru/iimm/article/download/1155/6742
Mukhina A.Y.; Kursk State Medical University
Medvedeva O.A.; Kursk State Medical University
Svishcheva M.V.; Kursk State Medical University
Shevchenko A.V.; Kursk State Medical University
Efremova N.N.; Kursk State Medical University
Bobyntsev I.I.; Kursk State Medical University
Kalutsky P.V.; Kursk State Medical University
Andreeva L.A.; Institute of Molecular Genetics, Russian Academy of Sciences
Myasoedov N.F.; Institute of Molecular Genetics, Russian Academy of Sciences
2020-01-31 20:34:15
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1155
селанк; микробиоценоз; иммобилизационный стресс; микробиота; дисбиоз
ru
It is currently accepted that stress significantly affects composition of microbiocenosis due to changes in permeability of intestinal barrier and pro-inflammatory effects. This, in turn, changes behavioral reactions, anxiety and stress response. In this regard, it seems promising to use regulatory peptide-based neurotropic drugs including Selank to correct stress-induced dysbiosis. Our study was aimed at assessing state of rat colon microbiocenosis in modelled chronic restraint stress and treated with Selankby using 65 Wistar male rats divided into five groups (per 13 rats in each): group 1 — rats injected with saline; group 2 — injected with saline and induced chronic restraint stress; group 3–5 — administered with Selank at dose of 80 μg/kg, 250 μg/kg and 750 μg/kg body weight, respectively, and induced chronic restraint stress. Quantitative and qualitative study of animal colon microbiota was carried out according to the method by L.I. Kafarskaja and V.M. Korshunov. Identification of microorganisms was carried out by using a Maldi Biotyper Microflex mass spectrometer (Bruker, United States). Microbial species-specific composition was presented as lg CFU/g mass of examined sample. For each identified microbial genus, the relative mean and frequency of occurrence were calculated. Statistical significance of differences in mean values was determined by using Student’s t-test. Chronic restraint stress in the experiment did not result in affecting dominant microbiota species in rat colon nor reduce their frequency, however, it significantly influenced examined parameters for commensal microbiota disturbing pattern of pathogenic bacterial strains. Use of Selank led to the reversing changes in composition of colonic microbiocenosis caused by stress model. Moreover, magnitude of parameters examined in experiment after applying Selank at dose of 750 μg/kg reached those in non-stressed animals. Thus, effects related to Selank administration may presumably be mediated due to both central and peripheral effects including immunotropic and anti-inflammatory activities which contributed to restoring colon microbiocenosis composition in stress model.
oai:iimmun.ru:article/45
2022-04-12T21:19:45Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/45
2022-04-12T21:19:45Z
Russian Journal of Infection and Immunity
Vol 1, No 2 (2011); 181-184
OPPORTUNICTIC ENTEROBACTERIACAE AS THE CAUSE OF THE ACUTE DIARRHEA AND GUT DISBIOSIS
Egorova S.A.; ФГУН «Санкт-Петербургский научно-исследовательский институт эпидемиологии и микробиологии имени Пастера» Роспотребнадзора, Санкт-Петербург
Makarova M.A.; ФГУН «Санкт-Петербургский научно-исследовательский институт эпидемиологии и микробиологии имени Пастера» Роспотребнадзора, Санкт-Петербург
Kaftyreva L.A.; ФГУН «Санкт-Петербургский научно-исследовательский институт эпидемиологии и микробиологии имени Пастера» Роспотребнадзора, Санкт-Петербург
2014-06-28 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/45
условно патогенные энтеробактерии; Klebsiella; факторы патогенности; острые кишечные заболевания
ru
Abstract. Strains of Klebsiella pneumonia isolated from faces were studied to detect virulence factors of “classical” enteric pathogens causing acute diarrhea (Shigella, Salmonella, diarreagenic Escherichia coli). We haven’t detect in Klebsiella the genes encode different virulence factors: the abilities to adherence (sfa, afa, aaf/1, eae), invasion (ipaH, ial), production of heat-lable toxins (elt, LT II), heat-stable toxins (st1, st2) and Shiga toxins (stx1, stx2). Klebsiella spp. is well-known pathogen of the opportunistic infections of urinary tract, blood, wound, respiratory tract. But this bacteria hasn’t virulence factors of diarreagenic Enterobacteriaceae and can’t cause acute diarrhea and other gut pathology.
oai:iimmun.ru:article/278
2022-04-12T15:14:22Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/278
2022-04-12T15:14:22Z
Russian Journal of Infection and Immunity
Vol 5, No 1 (2015); 85-88
IMMUNOLOGICAL STATUS OF PATIENTS WITH TUBERCULOSIS
Tarasova L.G.; 414000, Russia Federation, Astrakhan, Bakinskaya str., 121, Astrakhan State Medical Academy.
Strel’tsova E.N.; 414000, Russia Federation, Astrakhan, Bakinskaya str., 121, Astrakhan State Medical Academy.
2015-04-21 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/278
туберкулез;TNFα;IL-1β;IL-10;коллаген I типа;коллаген III типа;аутоантитела
ru
In 46 patients with active pulmonary tuberculosis who were treated in 2014, the features of the immunological status by defining standard immunograms and serum levels of TNFα, IL-1β, IL-10, as well as autoantibodies to collagen I and III type have been studied. It was established that the hyperproduction of T lymphocytes mainly Th2 link (increased relative to the Trel content of 17.4% of the Tabs — 30.4%, IL-1β — 32.6%, IL-10 — 43.5%) arises because of the decrease in the phagocytic activity of the cells (ratio of active phagocytes 1.98±1.07 at a rate of 2.5–2.9; percentage phagocytosis 56.35±13.89% at a rate of 65–95%). The increase in the content of B-lymphocytes (Brel — 84.8%, in the Babs — 43.5%) and circulating immune complexes, in some cases combined with decreased levels of immunoglobulins G, A, M, believe, intended to compensate the negative impact of Mycobacterium tuberculosis the body of the patient. The larger the specific process, the more intense the metabolism of connective tissue, stimulates the formation of type-specific autoantibodies involved in the elimination of connective tissue components. Detecting increased levels of autoantibodies to collagen type I — 50% and type III — 26.1%, probably due to the fact that in the initial stage of development of pulmonary tuberculosis «pulmonary collagen» is involved in the pathological process is less than I type.
oai:iimmun.ru:article/1635
2023-08-06T17:09:03Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/1635
2023-08-06T17:09:03Z
Russian Journal of Infection and Immunity
Vol 11, No 6 (2021); 1185-1189
Blockade of LIRs as a new approach for diagnostics and treatment of ATLL malignancy
https://iimmun.ru/iimm/article/download/1635/11365
Keikha M.; Mashhad University of Medical Sciences
Karbalaei M.; Jiroft University of Medical Sciences
2021-07-13 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1635
ATLL; HAM/TSP; HTLV-1; LIRs; злокачественность; иммунитет
en
In the new world of medicine, one of the main concerns in the field of infectious diseases has been focused on Human T-cell Leukemia Virus type 1 (HTLV-1). During the infection, lymphocyte inhibitory receptors (LIRs) play a prominent role in the occurrence of adult T-cell leukemia/lymphoma (ATLL). These receptors include LAG3, PD-1, TIGIT, CD160, TIM3, and 2B4. First, we have collected all microarray information on the profile of HTLV-1 infected patients from the Gene Expression Omnibus (http://www.ncbi.nlm.gov/geo) database until March 2020, in order to identify the microarray related to evolutionary development of LTRs during various phases of HTLV-1 infection in human peripheral blood CD4+ T cells by searching for keywords such as “Human T-lymphotropic virus type I (HTLV-1)”, “Homo sapiens”, “ATLL”, and “Whole genome sequencing”. Considering the main goal of the study, we have only assessed data related to Homo sapiens particularly CD4+ T cell lineage from human subjects infected with HTLV-1. We evaluated these receptors in ATLL patients compared to healthy control (HC) individuals and HTLV-1 infected-asymptomatic carriers (ASCs). Out of all 18 identified records, we only selected and analyzed three studies: GSE19080, GSE33615, and GSE57259, which satisfied inclusion criteria with proper quality analysis of ATLL vs. normal, ATLL vs. asymptomatic carrier as well as asymptomatic carrier vs. normal. Unfortunately, we could not analyze various stages of ATLL malignancy (acute, lymphomatous, chronic and smoldering) in all included studies due to the lack of sufficient information. Finally, based on Benjamini–Hochberg False discovery rate (FDR), the differentially expressed genes (DEGs) were selected for several categories. Hence, for the first time we demonstrated that the expression rate of LIRs in ATLL group was higher than either in asymptomatic carrier or healthy donor groups. As a conclusion, it seems that the blockade of LIRs has a pivotal role in diagnostics and treatment of ATLL malignancy.
oai:iimmun.ru:article/2055
2023-04-01T17:54:25Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/2055
2023-04-01T17:54:25Z
Russian Journal of Infection and Immunity
Vol 13, No 1 (2023); 167-170
Microbiological monitoring of nasal lavage fluid as a method for early detection and prevention of bacterial lung complications in a patient with cystic fibrosis
https://iimmun.ru/iimm/article/download/2055/14656
https://iimmun.ru/iimm/article/download/2055/14657
https://iimmun.ru/iimm/article/download/2055/14658
https://iimmun.ru/iimm/article/download/2055/14659
https://iimmun.ru/iimm/article/download/2055/14660
https://iimmun.ru/iimm/article/download/2055/14661
https://iimmun.ru/iimm/article/download/2055/14662
https://iimmun.ru/iimm/article/download/2055/14962
https://iimmun.ru/iimm/article/download/2055/74122
Kondratenko O.V.; Samara State Medical University of the Ministry of Healthcare of the Russian Federation
Lyamin A.V.; Samara State Medical University of the Ministry of Healthcare of the Russian Federation
Ereshchenko A.A.; Samara State Medical University of the Ministry of Healthcare of the Russian Federation
Antipov V.A.; Samara State Medical University of the Ministry of Healthcare of the Russian Federation
2023-04-01 18:41:50
Authors who publish with this journal agree to the following terms:
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Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/2055
муковисцидоз; назальный лаваж; нижние дыхательные пути; параназальные синусы; мокрота; MALDI-Tof масс-спектрометрия
Array
en
The severity of complications in cystic fibrosis are determined by microorganisms colonizing the lower airways. Paranasal sinuses can be a reservoir of aggressive pathogens. We have developed a method for collection and primary inoculation of nasal lavage fluid from cystic fibrosis patients for microbiological investigation. As a clinical case illustrating the feasibility of this technique, we describe the dynamics of the microflora composition in a patient with cystic fibrosis. The patient had a clinical and microbiological picture of P. aeruginosa eradication from the lung tissue, owing to which the antibacterial therapy was stopped. Six months later, the microflora in the nasal lavage fluid and sputum were assessed in parallel. The growth of P. aeruginosa (102 CFU/mL) but not P. aeruginosa in sputum was detected. To determine origin of this strain, the degree of genetic relationship between 5 strains obtained from the patient from 2008 to 2016 was assessed based on bacterial protein profiling. A typical strain of P. aeruginosa ATTS 27853 was used as a control. Strains isolated from the patient in 2009 and 2016 were identical suggesting that the antibacterial therapy led to eradication of P. aeruginosa in the lungs, but not in the upper airways. Four months later, the growth of P. aeruginosa was found in sputum. The patient was prescribed to use antibacterial drugs inhaled into paranasal sinuses. Repeated test performed 3 months later resulted in growth of P. aeruginosa 101 CFU/mL from nasal lavage fluid, but not from sputum. The patient was referred to a risk group on airway colonization by pathogen strains derived from the upper airway tract. The clinical example illustrates relevance of conducting a regular microbiological study of nasal lavage fluid in order to early identify clinically significant pathogens to prevent their spread to the lower airway tract.
oai:iimmun.ru:article/792
2023-08-06T17:26:40Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/792
2023-08-06T17:26:40Z
Russian Journal of Infection and Immunity
Vol 8, No 3 (2018); 388-392
COMPLIANCE OF ANTHRAX RECOMBINANT VACCINE PROTOTYPE WITH THE REQUIREMENTS TO IMMUNE-BIOLOGICAL PREPARATIONS
Mikshis N.I.; Russian Research Anti-Plague Institute “Microbe”.
Semakova A.P.; Russian Research Anti-Plague Institute “Microbe”.
Popova P.Y.; Russian Research Anti-Plague Institute “Microbe”.
Kudryavtseva O.M.; Russian Research Anti-Plague Institute “Microbe”.
Bugorkova S.A.; Russian Research Anti-Plague Institute “Microbe”.
Komissarov A.V.; Russian Research Anti-Plague Institute “Microbe”.
Germanchuk V.G.; Russian Research Anti-Plague Institute “Microbe”.
Popov Y.A.; Russian Research Anti-Plague Institute “Microbe”.
2018-11-02 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/792
Bacillus anthracis; сибирская язва; сибиреязвенные вакцины; рекомбинантные вакцины; протективный антиген; адъюванты; лиофилизация
ru
Current areal of anthrax among live stock and wild animals covers almost all the continents. The cause of human infection is conventionally considered to be the contact with diseased animals in the process of carrying for an animal, forced slaughter or further trimming of carcasses, as well as contact with infected raw materials of animal origin. Licensed vaccines has made an invaluable contribution to the improvement of epidemiological situation on anthrax, however, development of the vaccines complying with current scientific progress remains relevant. We have constructed a vaccine prototype containing recombinant protective antigen and S-layer EA1 protein and added state-of-the-art CpG 2006 adjuvant to the formulation. We have demonstrated the advantage of the lyophilized form of the preparation. Objective — obtainment of anthrax vaccine prototype in lyophilized state and assessment of the prototype compliance with the requirements to vaccine preparations. Materials and methods. Isolation of rPA and EA1 protein was carried out using producer-strain B. anthracis 55ΔTПА-1Spo– on an integrated end-to-end manufacturing line, including concentration, diafiltration and two-phase chromatography. CpG 2006 adjuvant was synthesized according to known sequences. Components were mixed and lyophilized in sublimation unit. Combination of 1% sucrose and 3% glycine was used as cryoprotector. Effectiveness and safety of the preparation were evaluated on the model of BALB/c mice and guinea pigs applying immunological, morphometric, and histological assays. Antibody titers in sera of immunized animals were evaluated using standard ELISA procedures. Protective properties were investigated through LD50 values for the test-strain used on immunized and control animals and immunity index. Results. We have performed complex investigation of the anthrax vaccine prototype, containing B. anthracis 55ΔTПА-1Spo– proteins as main and supplementary antigens, as well as CpG 2006 adjuvant, stabilizers and preservative agent. The prototype meets the requirements to immunobiological medicinal drugs by all physical-chemical properties. Our preparation does not have a toxic effect on the organism of laboratory animals in case of subcutaneous and intraperitoneal administration of single human dose. Pathomorphological study of guinea pigs’ organs immunized with double dose of the vaccine prototype has not revealed any evidence of damaging effect on the cells and tissues of macroorganism. The prototype protects BALB/c mice from the infection with B. anthracis 71/12 test-strain.
oai:iimmun.ru:article/1222
2023-08-06T17:10:22Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/1222
2023-08-06T17:10:22Z
Russian Journal of Infection and Immunity
Vol 10, No 4 (2020); 775-781
Clinical and immunological characteristics of bacterial pneumonia associated with HIV infection coupled to drug addiction
Puzyreva L.V.; Omsk State Medical University, Ministry of Healthcare of the Russian Federation
Mordyk A.V.; Omsk State Medical University, Ministry of Healthcare of the Russian Federation
Paneva M.A.; Omsk State Medical University, Ministry of Healthcare of the Russian Federation
2020-11-26 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1222
ВИЧ-инфекция; инфекции нижних дыхательных путей; пневмония; потребители инъекционных наркотиков
ru
Bacterial pneumonia holds the second place after respiratory tuberculosis in patients with HIV infection. In recent years, sexual transmission of HIV was replaced by injection drug route. It seems of high relevance to advance medical aid to patients with HIV infection and bacterial pneumonia depending on psychoactive substance use.Aim of study — assessment of clinical and immunological manifestations of bacterial pneumonia coupled to HIV infection with respect to verified injection drug use.Materials and methods. Clinical and immunological data collected from 224 patients with HIV infection and pneumonia were retrospectively analyzed: group 1 group — 70 patients with HIV infection, IDU, verified bacterial pneumonia; group 2 — 16 injecting drug users (IDU) with HIV infection and pneumonia of unverified etiology; group 3 — 65 patients with HIV and bacterial pneumonia of verified etiology without injection drug use, group 4 — 73 patients with HIV infection and bacterial pneumonia of unverified etiology, without injection drug use. The data obtained were analyzed by using software Statistica 13.3. Methods of descriptive statistics with calculation of nonparametric criterion — the Kruskall—Wallis test (H-criterion) and χ2 test — were used.Results. Immunological manifestations of HIV infection and bacterial pneumonia were characterized by decreased count of CD4+ cells paralleled with increased count of CD3+ and CD8+ cells at higher magnitude without injection drug use being also featured with peak viral load upon developing pneumonia. Bacterial pneumonia coupled to HIV infection showed clinical manifestations similar both in injecting drug users and non-users, proceeding in 10% cases during normothermia. Injection drug user patients often demonstrated clinical picture of pneumonia resembling those found in sepsis such as pain in the body, muscles, bone aches untypical to HIV-sexually infected subjects. In addition, systolic murmur on a heart top was more often auscultated in this patient group.Conclusion. Subjects self-considered healthy being at risk of sexually transmitted infections should examined for HIV. All subjects manifested with symptoms of the lower airway tract infections in admission department should not be rejected to be hospitalized and undergo chest X-ray examination.
oai:iimmun.ru:article/145
2022-04-12T21:20:53Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/145
2022-04-12T21:20:53Z
Russian Journal of Infection and Immunity
Vol 3, No 4 (2013); 355-358
MULTIDRUG-RESISTANCE AND PRESENCE OF CLASS 1 INTEGRONS IN CLINICAL ISOLATES OF SALMONELLA ENTERICA SEROTYPE ENTERITIDIS, CIRCULATING IN ARMENIA
Sedrakyan A.M.; Institute of Molecular Biology of National Academy of Sciences of the Republic of Armenia
Arakelova K.A.; Institute of Molecular Biology of National Academy of Sciences of the Republic of Armenia
Zakaryan M.K.; Institute of Molecular Biology of National Academy of Sciences of the Republic of Armenia
Hovhanisyan A.I.; Institute of Molecular Biology of National Academy of Sciences of the Republic of Armenia
Asoyan A.V.; “Nork” Clinical Hospital of Infectious Diseases, Ministry of Health of the Republic of Armenia, Yerevan
Gevorkyan Z.U.; “Nork” Clinical Hospital of Infectious Diseases, Ministry of Health of the Republic of Armenia, Yerevan
Mnatsakanyan A.A.; “Nork” Clinical Hospital of Infectious Diseases, Ministry of Health of the Republic of Armenia, Yerevan
Ktsoyan Z.A.; Institute of Molecular Biology of National Academy of Sciences of the Republic of Armenia
Boyajyan A.S.; Institute of Molecular Biology of National Academy of Sciences of the Republic of Armenia
Aminov R.; National Veterinary Institute, Technical University of Denmark, Copenhagen
2014-07-08 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/145
Salmonella enterica серотип Enteritidis; множественная лекарственная устойчивость; интегроны 1-го класса
ru
Abstract. The aim of this work was detection of class 1 integrons and their contribution to the antimicrobial resistance phenotypes in strains of subspecies enterica serotype Enteritidis. S. Enteritidis strains (n = 29) were isolated from patients with salmonellosis at “Nork” Clinical Hospital of Infectious Diseases, Yerevan, Republic of Armenia. High prevalence of multi-drug resistance (MDR) phenotypes was revealed and isolates with MDR phenotypes which are rare in the S. Enteritidis serotype were observed. Class 1 integrons were detected in 27,6% of isolates, with the prevalence of a variable region of 1000 bp. Occurrence of the MDR phenotype was more frequent in integron-positive isolates compared to integron-negative isolates of S. Enteritidis. Further studies are necessary to reveal the genetic background of MDR phenotypes and to estimate the genetic kinship among the isolates. Our results suggest a rapid and large-scale penetration of antibiotic resistance genes into populations of S. Enteritidis, which complicates infection control. More rigorous regulations should be imposed on antibiotic use, together with a vigilant epidemiological surveillance, to prevent the emergence and spread of MDR S. Enteritidis.
oai:iimmun.ru:article/1405
2023-08-06T17:10:12Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/1405
2023-08-06T17:10:12Z
Russian Journal of Infection and Immunity
Vol 11, No 2 (2021); 377-382
Immunostimulating effect of <i>Yersinia enterocolitica</i>-antigen conjugated gold nanoparticles
https://iimmun.ru/iimm/article/download/1405/7970
https://iimmun.ru/iimm/article/download/1405/7971
https://iimmun.ru/iimm/article/download/1405/7972
https://iimmun.ru/iimm/article/download/1405/7973
https://iimmun.ru/iimm/article/download/1405/7974
https://iimmun.ru/iimm/article/download/1405/7975
https://iimmun.ru/iimm/article/download/1405/7976
https://iimmun.ru/iimm/article/download/1405/7977
https://iimmun.ru/iimm/article/download/1405/9846
Staroverov S.А.; Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences; Saratov State Vavilov Agrarian University
Fomin А.S.; Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences
Gabalov K.P.; Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences
Ivaschenko S.V.; Saratov State Vavilov Agrarian University,
Manieson V.E.; Saratov State Vavilov Agrarian University
Dykman L.А.; Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences
2020-08-23 19:06:34
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1405
Yersinia enterocolitica; наночастицы золота; иммунизация; вакцинация; макрофаги; цитокины; иммуноанализ
Работа выполнена при финансовой поддержке Российского научного фонда (грант № 19-14-00077 «Иммуномодулирующий эффект наночастиц золота при вакцинации и иммунотерапии»).
ru
Yersinia enterocolitica is one of the most common causative agents of nosocomial infections and the causative agent of yersiniosis, an acute infectious disease of the gastrointestinal tract. Intestinal yersiniosis caused by Y. enterocolitica is a very pressing issue for clinical and veterinary medicine requiring use of sero- and immunodiagnostics to identify the causative agent in humans, animals and animal products. Gold nanoparticles represent one of the most popular antigen nanocarriers used for immunization and vaccination. The advantages of using gold nanoparticles as vaccine delivery vehicles are associated with their relatively small size promoting their tissue penetration, low toxicity and prolonged in vivo circulation, low cost, and reproducibility. Currently, the development of new gold nanoparticle-containing diagnostic tests and vaccines against viral, bacterial, parasitic infections, including bacteria of the genus Yersinia is underway. The aim of the study was to examine efficacy of 15 nm gold nanoparticles as an immunomodulator coupled to Y. enterocolitica-derived antigen for immunization and vaccination. Final conjugate construct was used to immunize laboratory animals. The methods of immunochemical analysis assessed sensitivity and specificity of the antibodies obtained. After that, protective effects were examined in animals vaccinated with antigen conjugates and comparison preparations. It was found that immunization with a Y. enterocolitica antigen-gold nanoparticle conjugate resulted in obtaining serum that specifically recognized yersiniosis ~35 and ~14 kDa proteins evaluated in Western blot analysis. It was shown that the specific sera cross-reacted with Y. pseudotuberculosis species, but not with intestinal group Escherichia coli, Salmonella enterica, Pseudomonas aeruginosa, Proteus vulgaris, suggesting the generic specificity of the antibodies obtained. To determine the protective effect, animals were vaccinated with antigen-gold nanoparticle conjugates and comparison preparations. Two weeks after the last vaccination, mice were challenged intraperitoneally with pathogenic strain Y. enterocolitica culture. The groups immunized with antigen-gold nanoparticle conjugate had 70—80% survival rate, whereas all control mice died. It was shown that Yersinia-derived antigen-gold nanoparticle conjugates possessed higher immunomodulating activity than unconjugated antigen. The antibodies obtained can be used for effective yersiniosis immunodiagnostics. Thus, Y. enterocolitica antigen-gold nanoparticle conjugates may serve as a basis for creating preventive vaccines.
oai:oai.iimmun.ru:article/1575
2020-08-07T07:04:12Z
iimm:SHORT
oai:iimmun.ru:article/187
2015-06-16T09:39:34Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/187
2015-06-16T09:39:34Z
Russian Journal of Infection and Immunity
Vol 4, No 3 (2014); 275-278
THE ANALYSIS OF IXODES TICKS INFESTATION WITH TBEV IN KIROV REGION
Bessolitsyna E.A.; Vyatka State University, Kirov
Volkov S.A.; Vyatka State University, Kirov
Stolbova F.S.; Vyatka State Agricultural academy, Kirov
Darmov I.V.; Vyatka State University, Kirov
2014-08-15 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/187
вирус клещевого энцефалита; иксодовые клещи; идентификация; обратная транскрипция; полимеразная цепная реакция
ru
The objects of this study are Ixodes ticks which were collected in different areas of Kirov province. The aim of the study is to determine the proportion of TBEV infected ticks using the reverse transcription and PCR, dependingon time, place, and methods of collection in the Kirov province as well as of ticks specific and sexual identity. The study found that from the two tick species that were tested only taiga tick (Ixodes persulcatus) but not the meadow thick (Dermacentor reticulatus) was the TBEV vector. Study also has shown that both males and females ticks can be the TBEV vectors. Moreover, it was proved the importance of ticks testing which were gathered not only from human but also from animals, primary from dogs, and from the plants.
oai:iimmun.ru:article/1330
2022-04-12T15:57:59Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/1330
2022-04-12T15:57:59Z
Russian Journal of Infection and Immunity
Vol 11, No 4 (2021); 784-788
Clinical and morphological case of developing liver cirrhosis associated with viral hepatitis in a young patient
https://iimmun.ru/iimm/article/download/1330/7366
https://iimmun.ru/iimm/article/download/1330/7367
https://iimmun.ru/iimm/article/download/1330/7368
https://iimmun.ru/iimm/article/download/1330/7369
https://iimmun.ru/iimm/article/download/1330/7370
https://iimmun.ru/iimm/article/download/1330/7371
https://iimmun.ru/iimm/article/download/1330/7372
https://iimmun.ru/iimm/article/download/1330/7373
https://iimmun.ru/iimm/article/download/1330/7374
https://iimmun.ru/iimm/article/download/1330/7375
https://iimmun.ru/iimm/article/download/1330/7376
https://iimmun.ru/iimm/article/download/1330/7377
https://iimmun.ru/iimm/article/download/1330/7378
https://iimmun.ru/iimm/article/download/1330/7379
https://iimmun.ru/iimm/article/download/1330/0
https://iimmun.ru/iimm/article/download/1330/10937
Vorobeva O.V.; I.N. Ulianov Chuvash State University
Lastochkin A.V.; I.N. Ulianov Chuvash State University; Republican Bureau of Forensic Medical Examination of the Ministry of Health of Chuvashia
2021-05-17 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1330
цирроз; печень; вирусный гепатит; отек; некронефроз; морфология
ru
The significance of chronic hepatitis is determined not only by the disease per se, but also by the increased risk of the formation of long-term adverse effects such as liver cirrhosis. Viral cirrhosis of the liver (as the outcome of chronic hepatitis B, C, B + D) comprise from 10% to 24.5% of all liver cirrhosis cases. Cirrhosis of the liver is considered as an irreversible stage of chronic hepatitis, more often observed in men above 40 years, but recently more and more its cases are found in young, working ones leading to disability. Due to the high liver regenerative potential, a prognosis for liver lesions of different etiologies may be rather favorable. As a result, early diagnostics, dynamic monitoring and the use of promising methods for treating liver pathology to stimulate its regeneration that compensates for lost liver functions are necessary. In the clinical and morphological case, pathomorphological changes in organs with liver cirrhosis, which developed as a result of viral hepatitis are described. The aim of the study was to describe pathomorphological changes in organs during liver cirrhosis, which developed due to viral hepatitis, which led to multiple organ failure in a young patient. Materials and methods. The analysis of the obtained accompanying medical documentation (outpatient card, medical history) was performed. The standard methodology of autopsy was used. To process histological sections of autopsy material, hematoxylin-eosin staining was used. Results. Histological examination in the liver shows development of portal tract fibrosis with lymphohistio cytic infiltration, formation of monolobular regenerated nodes, signs of edema, areas of demyelination, dystrophic changes in neurocytes, signs of acute renal failure, a combined profound change in the kidneys detected in the lungs and brain, which resulted in multiple organ failure and subsequent lethal outcome in a young patient. The early formation of portal hypertension with liver cirrhosis, esophageal and gastric varicose veins dilatation lead to fatal bleeding in half of patients. Therefore, timely diagnosed cirrhosis and hepatitis as their predecessors is one of the most relevant issues in medicine.
oai:iimmun.ru:article/1872
2023-01-06T21:08:14Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/1872
2023-01-06T21:08:14Z
Russian Journal of Infection and Immunity
Vol 12, No 6 (2022); 1191-1196
Pathomorphological features of acute respiratory distress syndrome in competing lung diseases: COVID-19 and sarcoidosis
https://iimmun.ru/iimm/article/download/1872/12503
https://iimmun.ru/iimm/article/download/1872/12504
https://iimmun.ru/iimm/article/download/1872/12505
https://iimmun.ru/iimm/article/download/1872/12508
https://iimmun.ru/iimm/article/download/1872/12509
https://iimmun.ru/iimm/article/download/1872/12510
https://iimmun.ru/iimm/article/download/1872/12511
https://iimmun.ru/iimm/article/download/1872/12512
https://iimmun.ru/iimm/article/download/1872/12513
https://iimmun.ru/iimm/article/download/1872/12514
https://iimmun.ru/iimm/article/download/1872/12515
https://iimmun.ru/iimm/article/download/1872/12516
https://iimmun.ru/iimm/article/download/1872/13200
Vorobeva О.V.; Chuvash State University named after I.N. Ulyanova
Gimaldinova N.E.; Chuvash State University named after I.N. Ulyanova
Romanova L.P.; Chuvash State University named after I.N. Ulyanova
2022-12-30 21:01:31
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1872
COVID-19; саркоидоз легких; пневмония; гипертензия; острый респираторный дистресс-синдром; гранулемыы
Array
ru
The COVID-19 pandemic is a worldwide problem. The clinical spectrum of SARS-CoV-2 infection varies from asymptomatic or paucity-symptomatic forms to conditions such as pneumonia, acute respiratory distress syndrome and multiple organ failure. Objective was to describe a clinical case of SARS-CoV-2 infection in the patient with sarcoidosis and cardiovascular pathology developing acute respiratory syndrome and lung edema. Material and methods. There were analyzed accompanying medical documentation (outpatient chart, medical history), clinical and morphological histology data (description of macro- and micro-preparations) using hematoxylin and eosin staining. Results. Lung histological examination revealed signs of diffuse alveolar damage such as hyaline membranes lining and following the contours of the alveolar walls. Areas of necrosis and desquamation of the alveolar epithelium in the form of scattered cells or layers, areas of hemorrhages and hemosiderophages are detected in the alveolar walls. In the lumen of the alveoli, a sloughed epithelium with a hemorrhagic component, few multinucleated cells, macrophages, protein masses, and accumulated edematous fluid were determined. Pulmonary vessels are moderately full-blooded, surrounded by perivascular infiltrates. Signs of lung sarcoidosis were revealed. Histological examination found epithelioid cell granulomas consisting of mononuclear phagocytes and lymphocytes, without signs of necrosis. Granulomas with a proliferative component and hemorrhage sites were determined. Giant cells with cytoplasmic inclusions were detected — asteroid corpuscles and Schauman corpuscles. Non-caseous granulomas consisting of clusters of epithelioid histiocytes and giant Langhans cells surrounded by lymphocytes were detected in the lymph nodes of the lung roots. Hamazaki–Wesenberg corpuscles inside giant cells were found in the zones of peripheral sinuses of lymph nodes. In the lumen of the bronchi, there was found fully exfoliated epithelium, mucus. Granulomas are mainly observed subendothelially on the mucous membrane, without caseous necrosis. Histological examination of the cardiovascular system revealed fragmentation of some cardiomyocytes, cardiomyocyte focal hypertrophy along with moderate interstitial edema, erythrocyte sludge. Zones of small focal sclerosis were determined. The vessels of the microcirculatory bed are anemic, with hypertrophy of the walls in small arteries and arterioles. Virological examination of the sectional material in the lungs revealed SARS-CoV-2 RNA. Conclusion. Based on the data of medical documentation and the results of a post-mortem examination, it follows that the cause of death of the patient R.A., 50 years old, was a new coronavirus infection COVID-19 that resulted in bilateral total viral pneumonia. Сo-morbidity with competing diseases such as lung sarcoidosis and cardiovascular diseases aggravated the disease course, led to the development of early ARDS and affected the lethal outcome.
oai:iimmun.ru:article/743
2022-04-12T15:22:33Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/743
2022-04-12T15:22:33Z
Russian Journal of Infection and Immunity
Vol 8, No 2 (2018); 230-234
STUDY OF ELISA TEST-SYSTEMS OF DIFFERENT FORMATS FOR DETECTION OF MEASLES VIRUS SPECIFIC IgM IN DIFFERENT GEOGRAPHIC ZONES
Bichurina M.A.; St. Petersburg Pasteur Institute,
Zheleznova N.V.; St. Petersburg Pasteur Institute,
Lavrentieva I.N.; St. Petersburg Pasteur Institute,
Antipova A.Y.; St. Petersburg Pasteur Institute,
Kulyashova L.B.; St. Petersburg Pasteur Institute,
Totolian A.A.; St. Petersburg Pasteur Institute,
2018-09-10 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/743
корь;специфические антитела;тест-системы;метод ИФА;«capture» формат;«indirect» формат;перекрестная реактивность
ru
Detection of the measles virus (MV) specific IgM antibodies in blood serum of patients is considered to be the main standard for the laboratory confirmation of measles diagnosis, the test being acknowledged by WHO. As it was demonstrated earlier the specific IgM antibodies as the marker of the acute MV infection were detected in 97.2–100% of blood serum samples from patients using the ELISA test-systems of the “capture” format (Microimmun Ltd. and Vector Best). In case when the ELISA test-system of the “indirect” format (Siemens, Germany) was used only 63.9% of these sera turned to be IgM positive. And on the contrary using the “indirect” format ELISA test-system Euroimmun, Germany, for detection of the MV specific IgM the false positive results were obtained.The aim of the study was the comparative evaluation of the different format ELISA test-systems used for the detection of the MV specific IgM antibodies in blood sera of patients and healthy adults collected in different geographic zones.Materials and methods. In total 108 serum specimens collected in 2015–2017 were studied: from healthy adult Guineans, residents of the Republic of Guinea (RG); patients aged 1–70 with the initial “infectious mononucleosis”, “infectious cytomegalovirus” and “rubella” diagnosis and taken from the bank of sera in the Subnational Measles/Rubella laboratory, StP Measles/Rubella RC in NWFR. The MV specific IgM antibodies were detected using the commercial ELISA test-systems “VectoMeasles-IgM” (Vector-Best, Russia) (“capture” format) and “Anti-Measles Virus ELISA IgM (NP)” (Euroimmun Medizinische Labordiagnostik AG, Germany) («indirect» format). The specific Epshtein-Barr Virus (EBV) IgM and IgG antibodies were detected with the commercial ELISA test-systems «DS-ELISA-anti-EBV-VCA-M», «DS-ELISA-anti-EBV-EA-G» and «DS-ELISA-anti-EBV-NA-G» (“Diagnostic Systems”, Russia).Results and discussion. The MV specific IgM antibodies were not revealed in the total of 108 blood serum samples from the healthy adults and patients, residents of the Russia and of the RG, with the “capture” format “VectoMeasles-IgM” ELISA test-system. The absence of the acute MV infection was also confirmed by the high measles immunity level (i.e. IgG MV antibodies titers) as well as by detection of the IgG antibodies of high avidity. At the same time in 6 of 108 total sera (5.5%) IgM MV antibodies were detected with the «indirect» format ELISA test system Euroimmun, Germany. In these 6 sera the EBV specific antibodies were also evidenced. The results obtained demonstrate the nonspecific reaction due to the possible reactivity with anti-EBV antibodies. Besides this the different percentage of the false positive reactions in sera from healthy adults, residents of the RG and residents of Russia was determined — 8.5±4.0% and 3.2±2.2% correspondently. Thus the preliminary results, and to get the final results for general conclusions increase of the total amount of the clinical specimens under studying is of extremely importance.
oai:iimmun.ru:article/1186
2023-08-06T17:10:44Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/1186
2023-08-06T17:10:44Z
Russian Journal of Infection and Immunity
Vol 10, No 3 (2020); 558-564
Advancing diagnostics of chronic paradontitis in children
Agarkov N.M.; South-West State University;
Belgorod State University
Makaryan A.S.; Russian Peoples Friendship University
Gontareva I.S.; Belgorod State University
2020-05-26 11:33:58
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1186
диагностика хронического пародонтита; системный иммунитет; дискриминантный метод; дети
ru
Chronic periodontitis in children and adolescents holds a lead place in morbidity pattern of dental pathology. Development of chronic periodontitis is accompanied by emergence of various complications in the maxillofacial region, leading to bite disturbance being of high relevance for pediatric patients. These and other complications are related to immune system immaturity in children and adolescents as well as virulence of microorganisms. However, the immunological changes developing in children with chronic periodontitis remain poorly studied. The aim of the work was to improve diagnostics of chronic periodontitis in children and adolescents based on informative parameters of systemic immunity and discriminative models taking into account such changes. We examined systemic immunity in 127 children and adolescents with chronic periodontitis, aged 12 to 16 years, by using flow cytometry and enzyme immunoassay. In control group, age-matched 108 patients lacking overt somatic and dental pathology were enrolled. Generation of mathematical models was carried out by using a discriminative approach, whereas informativeness was assessed in accordance with generally accepted formula. Relative and absolute count of peripheral blood CD13+ cells exert the peak informativeness holding the first and second ranking places with marked dominance of informativeness value for assessing relative amount of CD13+ cells are among immunological parameters in children with chronic periodontitis. High informativeness value evidencing about pronounced intensity of developed pathological changes and diagnostic significance for chronic periodontitis in children is intrinsic to the relative percentage of peripheral blood CD8+ cells being slightly lower than that one in absolute count of CD13+ cells. On the other hand, humoral immune-parameters were of lowest informative value among all analyzed immunological parameters in patients with chronic periodontitis serum referring to all antibody classes. Generated discriminative models for the most valuable immunological parameters ensure adequate medical diagnostics for chronic periodontitis in childhood. Diagnostic sensitivity for created mathematical models was high and reached 0.94, whereas diagnostic specificity — 0.92. Immunological examination of patients improves diagnostics of chronic periodontitis. It was found that patients with chronic periodontitis had lowered immune status peaking in decreased absolute and relative count of peripheral blood CD3+ lymphocytes. Finally, parameters of humoral immunity in children with chronic periodontitis were also reduced.
oai:iimmun.ru:article/114
2022-04-12T21:18:55Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/114
2022-04-12T21:18:55Z
Russian Journal of Infection and Immunity
Vol 2, No 4 (2012); 753-756
THE INFLUENCE OF DOUBLE IMMUNIZATION ON MORBIDITY OF MEASLES, MUMPS AND RUBELLA IN THE NORTH-WEST REGION OF RUSSIA
Lyalina L.V.; ФБУН НИИ эпидемиологии и микробиологии имени Пастера, Санкт-Петербург
Terentyeva J.V.; ФБУН НИИ эпидемиологии и микробиологии имени Пастера, Санкт-Петербург
Bichurina M.A.; ФБУН НИИ эпидемиологии и микробиологии имени Пастера, Санкт-Петербург
Antipova A.U.; ФБУН НИИ эпидемиологии и микробиологии имени Пастера, Санкт-Петербург
2014-07-04 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/114
иммунизация; заболеваемость; корь; эпидемический паротит; краснуха; надзор
ru
Abstract. The influence of double immunization on measles, mumps and rubella incidence in the North-West Federal District (North-West) Russia has been analyzed. Due to the vaccination of the population morbidity of these infections in the region has decreased significantly. The necessity to improve the surveillance system due to changes in the manifestations of the epidemic process particularly connected with increasing of proportion of adults among patients has been proposed.
oai:iimmun.ru:article/404
2022-04-12T15:18:36Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/404
2022-04-12T15:18:36Z
Russian Journal of Infection and Immunity
Vol 6, No 2 (2016); 179-183
GENOTYPES OF EXTENSIVELY DRUG-RESISTANT MYCOBACTERIUM TUBERCULOSIS STRAINS: CLINICAL AND EPIDEMIOLOGICAL FEATURES OF PULMONARY TUBERCULOSIS
Vasilieva N.R.; Research Institute of Phthisiopulmonology;
North-Western State Medical University named after I.I. Mechnikov
Vyazovaya A.A.; St. Petersburg Pasteur Institute
Zhuravlev V.Y.; Research Institute of Phthisiopulmonology
Solovieva N.S.; Research Institute of Phthisiopulmonology
Mokrousov I.V.; St. Petersburg Pasteur Institute
Narvskaya O.V.; Research Institute of Phthisiopulmonology;
St. Petersburg Pasteur Institute
2016-06-25 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/404
Mycobacterium tuberculosis;широкая лекарственная устойчивость;сполиготипирование;генотип Beijing;клинико-эпидемиологическая характеристика
ru
Here, we present clinical and epidemiological analysis of 85 Russian cases of pulmonary tuberculosis caused by an extensively drug-resistant M. tuberculosis strains. As defined by spoligotyping, M. tuberculosis strains belonged to the following genetic families: Beijing — 81.2%, which significantly exceeds the prevalence rate of this genotype (50%) in M. tuberculosis population across Russia; LAM — 14.1% and Ural — 4.7%. Among patients infected with Beijing strains prevailed alcohol and tobacco abused males; the main source of infection were family and penitentiary contacts. This group of patients has been characterized by a variety of clinical forms of lung disease with the prevalence of fibro-cavernous tuberculosis and a significant proportion of patients with interrupted treatment. Regardless of the M. tuberculosis strain genotype, the extensively drug-resistant pulmonary tuberculosis is characterized by severe course leading to the chronic disease with the relapses and poor response to anti-tuberculosis treatment, requiring repeated hospitalizations and surgical treatments.
oai:iimmun.ru:article/1838
2023-11-19T09:12:09Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/1838
2023-11-19T09:12:09Z
Russian Journal of Infection and Immunity
Vol 12, No 2 (2022); 386-390
Species diversity among the genus <i>Streptomyces</i> members isolated from clinical material
https://iimmun.ru/iimm/article/download/1838/12179
https://iimmun.ru/iimm/article/download/1838/12180
https://iimmun.ru/iimm/article/download/1838/12181
https://iimmun.ru/iimm/article/download/1838/12182
https://iimmun.ru/iimm/article/download/1838/12183
https://iimmun.ru/iimm/article/download/1838/12184
https://iimmun.ru/iimm/article/download/1838/12185
https://iimmun.ru/iimm/article/download/1838/12186
https://iimmun.ru/iimm/article/download/1838/12613
https://iimmun.ru/iimm/article/download/1838/13399
Lyamin A.V.; Samara State Medical University of the Ministry of Health of the Russian Federation
Tereshchenko V.S.; Samara State Medical University of the Ministry of Health of the Russian Federation
Zhestkov A.V.; Samara State Medical University of the Ministry of Health of the Russian Federation
Ismatullin D.D.; Samara State Medical University of the Ministry of Health of the Russian Federation
2022-05-13 14:39:47
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1838
стрептомицеты; микобактерии; клинический материал; туберкулез; идентификация; масс-спектрометрия
Array
ru
Recently, both in Russia and around the world, the number of cases detecting acid-fast microbial members from the order Actinomycetales while developing human bacterial infections has been increased. The most important pathogens in this bacterial order are the members from the families Mycobacteriaceae, Nocardiaceae, Gordoniaceae, Tsukamurellaceae, Promicromonosporaceae, Brevibacteriaceae, Streptomycetaceae. This work is devoted to analyzing prevalence and species diversity of representatives of the family Streptomycetaceae from the genus Streptomyces isolated from clinical material upon examining for tuberculosis. There were examined 865 cultures of clinical material samples while examining for tuberculosis, in which signs of growth of contaminating microflora were revealed, as well as 316 cultures of clinical material samples obtained during examination for tuberculosis, in which signs of growth of non-tuberculosis mycobacteria (NTM) were detected. The material was collected from January 2016 to January 2019. Samples with signs of growth of contaminating microflora allowed to identify 1,093 strains, samples with signs of growth of NTM — 352 strains. Among them, the number of representatives of the genus Streptomyces comprised 39 strains. All Streptomyces strains were isolated from sputum. Variety of isolated Streptomyces: S. phaeochromogenes (13 strains), S. albus (1 train), S. avidinii (1 strain), S. badius (2 strains), S. chartreusis (2 strains), S. griseus (1 strain), S. hirsutus (2 strains), S. lavendulae (3 strains), S. violaceoruber (10 strains). Species identification was not possible for 4 strains. Analyzing the data obtained, it is possible to draw a conclusion about the moderate distribution of representatives of the genus Streptomyces in pattern of contaminating microflora while examining for tuberculosis. In pattern structure of isolated microorganisms, Streptomyces accounted for 3.3%. Among the microflora isolated from culture with signs of NTM growth, Streptomyces was presented by single strains. However, it should be noted that Streptomyces was the dominant group among acid-resistant actinomycetes in pattern of contaminating microflora and accounted for 38.3%. Taking into account the fact that a significant proportion of them were isolated as microbial associations, it can be concluded that Streptomyces turned out to be classical contaminants in this case. Nevertheless, we believe that the isolation of Streptomyces in association with clinically significant NTMs can be considered as an unfavorable factor due to the fact that antibiotic resistance genes are widespread among Streptomyces and may be transmitted to other types of microorganisms from the group of acid-resistant actinomycetes, including mycobacteria. Thus, the clinical material examining for tuberculosis is an interesting research object from which various representatives of acid-fast actinomycetes, including Streptomyces, can be isolated.
oai:iimmun.ru:article/8060
2023-12-15T21:47:54Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/8060
2023-12-15T21:47:54Z
Russian Journal of Infection and Immunity
Vol 13, No 5 (2023); 979-984
Inpatient covid-19-related comorbidities and ct-semiotics
https://iimmun.ru/iimm/article/download/8060/73986
https://iimmun.ru/iimm/article/download/8060/73988
https://iimmun.ru/iimm/article/download/8060/73989
https://iimmun.ru/iimm/article/download/8060/73990
https://iimmun.ru/iimm/article/download/8060/73991
https://iimmun.ru/iimm/article/download/8060/73992
https://iimmun.ru/iimm/article/download/8060/73993
https://iimmun.ru/iimm/article/download/8060/73994
https://iimmun.ru/iimm/article/download/8060/73996
https://iimmun.ru/iimm/article/download/8060/74156
https://iimmun.ru/iimm/article/download/8060/74157
https://iimmun.ru/iimm/article/download/8060/74158
https://iimmun.ru/iimm/article/download/8060/153590
https://iimmun.ru/iimm/article/download/8060/153597
https://iimmun.ru/iimm/article/download/8060/157804
Titova A.V.; Smolensk State Medical University
Givoyno U.K.; Smolensk State Medical University
Denisova T.P.; Smolensk State Medical University
2023-11-30 23:31:33
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/8060
COVID-19; полисегментарная пневмония; дыхательная недостаточность; коморбидные состояния; КТ-диагностика; осложнение заболевания
Array
ru
Introduction. In the Russian Federation, registration of individual COVID-19 cases started at the end of January 2020 that markedly increased in the second half of March. At the moment, the situation with COVID-19 is unstable. As of 2023, COVID-19 incidence in descending order is as follows: USA (104 958 987 people), India (44 684 775 people) and France (39 582 057 people). Russia ranked 10th regarding total COVID-19 incidence (22 137 084 people, including 395 727 fatalities). The infection is able to “overlap” with the underlying pathology, thereby worsening patient’s condition up to death. Lung multislice computed tomography (MSCT) is recommended for all patients with COVID-19. If unavailable, a chest X-ray is performed. The aim of the study was to identify the most frequent comorbid diseases and changes on radiological scan in patients with COVID-19. Materials and methods. The study was carried out at the Infectious Disease Department No. 1, Regional Public Health Institution “Clinical Hospital No. 1” Smolensk. For this, there were analyzed 69 hospital patient records with basic diagnosis — coronavirus infection. The data of anamnesis and additional instrumental investigations were taken into account.
Results. 69 patients aged between 18 and 91 years were included in the study. Respiratory failure was not observed in 75.81% of cases. A group of patients (66.67%) with comorbidities of organs and systems was identified, among which most common were: arterial hypertension (95.65%), coronary heart disease (78.26%), diabetes mellitus (30.43%), grade 1–2 obesity (17.39%), chronic hepatitis (17.39%). Diseases of the gastrointestinal tract and respiratory system were less prevalent. The rate of reported complications is as follows: bilateral polysegmental pneumonia — 72.46%; unilateral polysegmental pneumonia — 2.8%; pleurisy — 4.35%; unilateral hydrothorax — 1.45%. Each of the inpatients underwent radiological examination during hospitalisation. X-ray examination accounted for a smaller proportion (39.13%) and multispiral chest computed tomography (MSCT) — for a bigger percentage (60.87%). Chest MSCT at the beginning of inpatient treatment visualized CT-2 stage (64.29%) in the vast majority, less — CT-1 (26.19%), least frequently — CT-3 (7.14%) and CT-4 (2.38%). Before patient discharge, an average of 12.16% improvement in the radiological picture was found in patients with initial CT-1 stage, 14% — in CT-2, 12% and 26% — in CT-3 and CT-4, respectively. Conclusion. The moderate disease course without respiratory depression prevailed among the patients. Co-morbidities were found mainly in the anamnesis of persons over 50 years old. The most frequent clinical complication of coronavirus infection was bilateral pneumonia. After patient’s hospitalization, clinical and instrumental picture gradually improved, which was confirmed by radiological data.
oai:iimmun.ru:article/903
2022-04-12T15:48:02Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/903
2022-04-12T15:48:02Z
Russian Journal of Infection and Immunity
Vol 9, No 3-4 (2019); 607-611
Humoral and cellular immunity to measles and rubella virus antigens in healthy subjects
https://iimmun.ru/iimm/article/download/903/4748
https://iimmun.ru/iimm/article/download/903/4749
https://iimmun.ru/iimm/article/download/903/4750
https://iimmun.ru/iimm/article/download/903/4751
https://iimmun.ru/iimm/article/download/903/4752
https://iimmun.ru/iimm/article/download/903/4753
https://iimmun.ru/iimm/article/download/903/4754
https://iimmun.ru/iimm/article/download/903/4755
Smerdova M.A.; G.N. Gabrichevsky Research Institute for Epidemiology and Microbiology
Toptygina A.P.; G.N. Gabrichevsky Research Institute for Epidemiology and Microbiology;
Lomonosov Moscow State University
Andreev Y.Y.; G.N. Gabrichevsky Research Institute for Epidemiology and Microbiology
Sennikova S.V.; G.N. Gabrichevsky Research Institute for Epidemiology and Microbiology
Zetkin A.Y.; Main Center of State Sanitary and Epidemiological Supervision (of Special Purpose), Ministry of Defense of the Russian
Federation
Klykova T.G.; Main Center of State Sanitary and Epidemiological Supervision (of Special Purpose), Ministry of Defense of the Russian
Federation
Belyakov S.I.; Military Unit 83466
2019-11-15 20:42:15
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/903
корь; краснуха; антитела; популяционный иммунитет; клеточный иммунитет; серомониторинг
ru
An issue of eradicating measles and rubella virus-induced infections currently remains unresolved, despite existing effective methods for specific prophylaxis and WHO’s commitment to a mass vaccination policy. While improving epidemic situation, analysis of new challenges, such as measles incidence in adults, especially in adults vaccinated in childhood, is of particular interest. The aim of the study was to analyze serum measles and rubella virus-specific IgG antibodies in young healthy people and estimate antigen-specific cellular immune response in seronegative subjects. There were examined 100 healthy adults aged 18–30 years old. Level of serum specific IgG was measured by ELISA (Vector-Best, Russia). Antigen-specific cellular immune response was assessed by magnitude of surface CD107a expression on CD8hi T cells challenged by measles and rubella virus-derived antigens. It was found that average level of antibodies against rubella virus comprised 175.5 IU/ml, 49% of which recovered after rubella, 46% were vaccinated, whereas 5% subjects contained no virus-specific antibodies. In addition, mean level of anti-measles virus antibodies was below protective magnitude, among which 1% subjects recovered after measles, 31% displayed post-vaccination immunity, 55% subjects were seronegative, and 13% had equivocal levels of specific antibodies. Thus, 68% subjects were unprotected against measles virus based on the level of serum virus-specific antibodies. Moreover, 40 out of 68 subjects were vaccinated against measles in childhood. Additional screening adult subjects for intensity of measles and rubella virus-specific cellular immunity demonstrated that 57.37% of them contained peripheral blood CD8 T cells against measles virus and 59.01% — against rubella virus. Further analysis allowed to identify 4 subgroups displaying: 1) high level of virus-specific antibodies and T cells; 2) neither antibodies nor specific T-cells reaching as low as 20% of baseline group; 3) high antibody level combined with low amount of specific T cells; and 4) low antibody level combined with high level of specific T cells. thus, it may be assumed that cellular and humoral immune arms are maintained independently and being active for a long term after vaccination. Preserving a specific T-cell immunity seems to provide protection against infection, thereby accounting for the lack of measles manifestation in all seronegative subjects.
oai:iimmun.ru:article/1467
2022-04-12T15:55:03Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/1467
2022-04-12T15:55:03Z
Russian Journal of Infection and Immunity
Vol 11, No 1 (2021); 191-196
Clinical and laboratory characteristics of influenza infection in hospitalized adult patients during the 2018-2019 epidemic season
https://iimmun.ru/iimm/article/download/1467/8545
https://iimmun.ru/iimm/article/download/1467/8546
https://iimmun.ru/iimm/article/download/1467/8547
https://iimmun.ru/iimm/article/download/1467/8548
https://iimmun.ru/iimm/article/download/1467/8549
https://iimmun.ru/iimm/article/download/1467/8550
https://iimmun.ru/iimm/article/download/1467/8557
https://iimmun.ru/iimm/article/download/1467/10246
Voloshchuk L.V.; St. Petersburg Pasteur Institute
Go A.A.; St. Petersburg Pasteur Institute
Pisareva M.M.; Smorodintsev Research Institute of Influenza
Guzhov D.A.; Smorodintsev Research Institute of Influenza
Bichurina M.A.; St. Petersburg Pasteur Institute
Petrova P.A.; St. Petersburg Pasteur Institute; Smorodintsev Research Institute of Influenza
2021-02-28 20:22:47
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
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Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1467
грипп; вирусы гриппа; эпидемиология; клиническая картина; осложнения; пневмония
ru
Despite the success in prevention and therapy, influenza remains a mass disease with mortality rate up to 0.01—0.2% worldwide.Purpose. Conducting clinical and laboratory analysis of influenza infection cases and evaluating their etiological significance in adult hospitalized patients during 2018—2019 epidemic season. Materials and methods. There were analyzed 569 case histories of patients hospitalized at the Clinical Infectious Diseases Hospital named after S.P. Botkin. Patients were examined by PCR that resulted in verified influenza virus in 260 cases. Nasopharyngeal swabs collected from 36 patients were examined by virological method on MDCK cell culture. 24 influenza virus strains were isolated and identified.Results. The study allowed to identify a viral landscape represented by influenza viruses A and B found in 98.5% and 1.5% cases, respectively. Influenza viruses isolated on cell culture in 50% of cases were identified. Among the influenza viruses isolated on cell culture there were identified serotype A influenza viruses (H1N1) closely related to the pandemic influenza A (H1N1)pdm09. Some isolates (41.7%) belonged to serotype A (H3N2), which were related to strain A/Singapore/16-0019/16. Influenza B virus strain of the Victoria lineage isolated from a hospitalized patient possessed a triple deletion in hemagglutinin gene, which antigenic properties substantially differed from those of the influenza virus strain being included into current influenza vaccines. Upon admission, the condition of most patients was estimated as moderate (males — 48.7%, females — 51.3%). The median patient age was 35 years old, with comorbidities being registered in 50% cases. The clinical picture for 2018—2019 seasonal influenza displayed no distinctive features as compared to previous epidemic seasons. The duration of intoxication and catarrhal syndrome was 4.3±0.13 and 6.9±0.29 days, respectively, with median body temperature ranging within 39.2±0.06°С. All patients received standard pathogenetic therapy. Complications were noted in 86.7% cases such as pneumonia — 11.1%, sinusitis — 6.9%, bronchitis — 56.9%. The bed day length was 5.93±0.29, no lethal outcomes were recorded.Conclusion. It was found that influenza A viruses were dominant in patients observed comprising up to 98.5% cases, whereas influenza viruses B were found in as few as 1.5% patients. The clinical picture was characterized by severe intoxication and catarrhal syndrome, being frequently associated with complications.
oai:iimmun.ru:article/1992
2023-01-06T21:08:11Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/1992
2023-01-06T21:08:11Z
Russian Journal of Infection and Immunity
Vol 12, No 6 (2022); 1181-1185
The oral cavity as a site for developing a heterogeneous bacterial population in patients with cystic fibrosis
https://iimmun.ru/iimm/article/download/1992/13848
https://iimmun.ru/iimm/article/download/1992/13849
https://iimmun.ru/iimm/article/download/1992/13850
https://iimmun.ru/iimm/article/download/1992/13851
https://iimmun.ru/iimm/article/download/1992/13870
https://iimmun.ru/iimm/article/download/1992/13871
https://iimmun.ru/iimm/article/download/1992/13875
https://iimmun.ru/iimm/article/download/1992/13876
https://iimmun.ru/iimm/article/download/1992/13877
https://iimmun.ru/iimm/article/download/1992/14258
Kondratenko O.V.; Samara State Medical University of the Ministry of Healthcare of the Russian Federation
Saburova M.S.; Samara State Medical University of the Ministry of Healthcare of the Russian Federation
2022-12-30 21:01:26
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1992
муковисцидоз; полость рта; мокрота; синегнойная инфекция; масс-спектрометрия; гетерогенность
Array
ru
Cystic fibrosis is an important medical and social problem. Despite significant advances in modern science and practice, bacterial complications are still among the main causes of patient mortality. One of the crucial bacterial pathogens in cystic fibrosis is Pseudomonas aeruginosa, which can lead to rapidly decreased pulmonary function. At the same time, the lungs are not the only site for microbial colonization. The oral cavity can also serve as a reservoir for initial entry and adaptation of microbial strains, which, however, were only sparsely investigated. A clinical case of isolated heterogeneous microbial population of P. aeruginosa from various loci of the oral cavity and sputum of a patient with cystic fibrosis is described. A proteomic mass-spectrum analysis obtained from eleven isolates as well as their mutual comparison with the control strain of P. aeruginosa ATCC 27853 visualized with CCI matrix was performed. As a result, a high level of heterogeneity of the studied population was revealed, while high CCI Score values were obtained only for individual pairs of isolates. It was shown that the bacterial strains isolated from the patient sputum turned out to be more homogeneous in protein profiles than cultures detected from various oral sites. At the same time, in case of isolated several morphotypes of P. aeruginosa from single oral site, no closely related strains were found. Thus, it is shown that the oral cavity is more important viewed from microbial dissociation in cystic fibrosis, which may be due to more diverse microecological conditions. Representatives of the microbiological community can recolonize the tracheobronchial tree through microaspiration, thereby contributing to the maintenance of infectious inflammation in the lower respiratory tract and partially accounting for ineffectiveness of nebulized antibacterial therapy. At the same time, clones adapted to the lung tissue upon coughing up sputum are able to colonize oral sites. The data obtained actualize the question of the need for a comprehensive microbiological approach while examining patients to increase effectiveness of eradication measures in cystic fibrosis.
oai:iimmun.ru:article/601
2022-04-12T15:21:18Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/601
2022-04-12T15:21:18Z
Russian Journal of Infection and Immunity
Vol 7, No 4 (2017); 419-424
PROBIOTIC-BASED SANITATION AS ALTERNATIVES TO CHEMICAL DISINFECTANTS
Afinogenova A.G.; St. Petersburg Pasteur Institute, St. Petersburg;
St. Petersburg State University, St. Petersburg
Kraeva L.A.; St. Petersburg Pasteur Institute, St. Petersburg
Afinogenov G.E.; St. Petersburg State University, St. Petersburg
Veretennikov V.V.; St. Petersburg State University, St. Petersburg
2018-01-20 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/601
пробиотики; пробиотическая очистка; химические дезинфектанты; очистка поверхностей; устойчивость патогенов к дезинфектантам
ru
It is known that any surfaces in environment of healthcare organizations are a reservoir for microorganisms and contribute to transmission of pathogens, increasing the risk of cross-contamination through indirect contact with the patient. Wide and not always rational use of chemical disinfectants carries risks for the safety of the environment, the patient and medical staff. In this regard, there remains the search for alternative methods of purification and decontamination of abiotic surfaces in health care facilities. Tested the tool for cleaning areas that contain 3 strains of the genus Bacillus: B. subtillis, B. licheniformis, B. pumilus. Were treated areas of the medical centre in St. Petersburg Pasteur Institute: the treatment room (experience) and the office of the gynecologist (control with conventional cleaning and disinfection). Within 30 days of the sowing of samples of swabs from surfaces were made of 3 Petri dishes with the “blood” agar-based environment “agar of Givental–Witch” debilitating touch (gold method). Isolated cultures of bacteria identified by microscopy, biochemical typing and MALDI-TOF MS (Bruker). In control and the experience in the 1st day of observation prior to the cleaning sown Enterococcus faecium 104 –105 CFU/ml. The 2nd day of observation in the control was dominated by E. faecium, then his growth stopped. For the 2nd–4th day watched the growth of Enterobacteriaceae to 106 CFU/ml, in the future, the number was 10 CFU/ml until the end of the observation period (30 days). The 5th day in the control revealed the growth of bacteria of the genus Staphylococcus spp. mainly S. epidermidis. The experience from the 2nd day of observation revealed a significant increase in Bacillus spp. at 105 CFU/ml, then before the end of the experiment, the number was 102 –103 CFU/ml. In the 1st–2nd day revealed growth of E. faecium, and then it stopped. For the 2nd–4th day watched the growth of Enterobacteriaceae to the level of 106 CFU/ml, on the 8th day — to 102 CFU/ml, the growth of conditionally pathogenic microflora was absent. Cleaning of the premises on the basis of probiotic bacteria of the genus Bacillus inhibits the growth of sanitary-indicative microorganisms compared to control. Thus, the means of probiotic treatment did not have a biocidal effect, but inhabiting the surface, they inhibit the growth and proliferation of opportunistic bacteria.
oai:iimmun.ru:article/1167
2023-08-06T17:11:16Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/1167
2023-08-06T17:11:16Z
Russian Journal of Infection and Immunity
Vol 10, No 1 (2020); 187-192
Influence of <i>Helicobacter pylori</i> on cytokine regulation in chronic atrophic gastritis
https://iimmun.ru/iimm/article/download/1167/5687
https://iimmun.ru/iimm/article/download/1167/5688
https://iimmun.ru/iimm/article/download/1167/5689
https://iimmun.ru/iimm/article/download/1167/5690
https://iimmun.ru/iimm/article/download/1167/5691
https://iimmun.ru/iimm/article/download/1167/5692
Smirnova O.V.; Research Institute of Medical Problems of the North FIC KSC, SB RAS
Sinyakov A.A.; Research Institute of Medical Problems of the North FIC KSC, SB RAS
2020-04-05 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1167
Helicobacter pylori; хронический гастрит; предраковые состояния желудка; цитокины; иммуноферментный анализ
ru
At present, the level of Helicobacter pylori infection is determined by geographic area, gender and age of the examined individuals, and can reach up to 95% of the total population. Environmental adaptation of H. pylori is exhibited in its ability to adhere to the gastric mucosal epithelium and modulated expression of its own virulent factors. Current concepts implicate that H. pylori can survive inside epithelial cells, evading host immune response. Cytokines are produced by immune cells and act to regulate its major stages. A cytokine cascade launched after Helicobacter pylori infection triggers immune reactions, progression of chronic inflammatory and destructive processes in the gastric mucosa. The role of cytokines in precancerous diseases of the stomach is ambiguous because, on the one hand, they activate immune response aimed at eliminating the pathogen, whereas on the other hand, they do contribute to the disease progression. The aim of our study was to examine profile of some cytokines and features of cytokine regulation in H. pylori-infected middle-aged males with chronic gastritis (CG) as well as chronic atrophic gastritis (CAG). In patients with CG with H. pylori, CAG and CAG with H. pylori, an increase in the cytokine IL-2 was observed that might contribute to augmented damaging effect of cytotoxic lymphocytes, as well as implementation of antitumor effect. CAG with H. pylori was featured with IL-8 hyperproduction, which resulted in increased absolute numbers of band neutrophils in peripheral blood and their decreased phagocytic activity evidencing about altered host defense mechanisms. There was increased amount of IFNy involved in recognition of malignantly transformed cells and upregulated expression of the major histocompatibility complex molecules on antigen-presenting cells. In patients with CG with H. pylori and CAG with H. pylori, production of IL-4 was increased, which might serve as a contributing factor to the chronicity of H. pylori-associated diseases. Overproduction of type 1 and type 2 cytokines indicates about activated Th1 and Th2 type immune reactions in H. pylori-associat-ed CG. A potent pro-inflammatory cytokine cascade triggers inflammatory changes in gastric mucosa with developing neutrophil infiltration and lymphocyte activation. Damage and death of epithelial cells upon inflammation form erosive and ulcerative defects, or changes manifested as gastric mucosal atrophy, metaplasia and neoplasia. The data obtained may be used as additional diagnostic criteria in early diagnostics of precancerous stomach diseases.
oai:iimmun.ru:article/72
2022-04-12T21:20:02Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/72
2022-04-12T21:20:02Z
Russian Journal of Infection and Immunity
Vol 1, No 4 (2011); 373-377
SIGNIFICANCE OF IMMUNE MECHANISMS IN PATHOGENESIS OF SOME ACUTE AND CHRONIC VIRAL INFECTIOUS DISEASES
Ivanis V.A.; ГОУ ВПО Владивостокский государственный медицинский университет Росздрава, г. Владивосток
Markelova E.V.; ГОУ ВПО Владивостокский государственный медицинский университет Росздрава, г. Владивосток
Sklyar L.F.; ГОУ ВПО Владивостокский государственный медицинский университет Росздрава, г. Владивосток
2014-07-02 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/72
патогенез; инфекции; иммунные нарушения; цитокины
ru
Abstract. In the last years immunological aspects are studying in the pathogenesis of infectious diseases. The influence of immune response to the infectious processes of acute and chronic virus infectious (Hemorrhagic Fever with Renal Syndrome and Chronic Viral Hepatitis C, as an example) has been revealed. Misbalance of system and local cytokine status characterizes the direct influence of immune system to all organs and systems of human organism. It determines the severity of the disease and its outcomes.
oai:iimmun.ru:article/354
2023-08-06T17:27:02Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/354
2023-08-06T17:27:02Z
Russian Journal of Infection and Immunity
Vol 5, No 4 (2015); 373-376
SELECTION OF PHYLOGENETICALLY CLOSELY-RELATED YERSINIA PESTIS STRAINS DIFFERING IN THEIR VIRULENCE FOR GUINEA PIGS
Anisimov N.V.; State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russian Federation
Kombarova T.I.; State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russian Federation
Platonov M.E.; State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russian Federation
Ivanov S.A.; State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russian Federation
Sukhova M.A.; State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russian Federation
Dentovskaya S.V.; State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russian Federation
Anisimov A.P.; State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russian Federation
2016-02-15 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/354
Yersinia pestis;полевочьи штаммы;избирательная вирулентность;морские свинки;анимализация;тестикулярные пассажи
ru
Genomic, transcriptome or (and) proteomic comparison of closely related virulent and avirulent microbial strains underlies the search for new pathogenicity factors, potential molecular targets for etiotropic therapy, vaccine prevention and immunotherapy of infectious diseases. This investigation was aimed in testing the ability of method of testicular animalization to select phylogenetically close pairs of Y. pestis strains, which dramatically differ in their pathogenicity for guinea pigs, from the populations of as a rule subcutaneously avirulent for guinea pigs “vole” strains of the plague pathogen. Animalization of Y. pestis cultures were performed on guinea pig males by fourfold testicular passage with reducing infective dose. There was no correlation between the ability to cause generalized infectious process (death) after testicular and subcutaneous infection of guinea pigs, but testicular passages made it possible to enrich bacterial culture with a portion of microbes displaying high virulence after subcutaneous infection of this animal species. The methodical approach under study can be successfully applied for selection of pairs of phylogenetically closely related bacterial strains, dramatically differing in their degrees of selective virulence.
oai:iimmun.ru:article/1751
2023-11-16T16:09:31Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/1751
2023-11-16T16:09:31Z
Russian Journal of Infection and Immunity
Vol 12, No 1 (2022); 193-196
Antimicrobial activity of polyhexamethylene guanidine hydrochloride derivatives against multiresistant microbial strains
https://iimmun.ru/iimm/article/download/1751/11164
https://iimmun.ru/iimm/article/download/1751/11165
https://iimmun.ru/iimm/article/download/1751/11166
https://iimmun.ru/iimm/article/download/1751/11167
https://iimmun.ru/iimm/article/download/1751/11168
https://iimmun.ru/iimm/article/download/1751/12161
Ochirov O.S.; Baikal Institute of Nature Management of the Siberian Branch of the Russian Academy of Sciences
Burasova E.G.; Republican Clinical Hospital named after N.A. Semashko
Stelmakh S.A.; Baikal Institute of Nature Management of the Siberian Branch of the Russian Academy of Sciences
Grigor'eva M.N.; Baikal Institute of Nature Management of the Siberian Branch of the Russian Academy of Sciences
Okladnikova V.O.; Baikal Institute of Nature Management of the Siberian Branch of the Russian Academy of Sciences
Mognonov D.M.; Baikal Institute of Nature Management of the Siberian Branch of the Russian Academy of Sciences
2021-12-03 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1751
полигуанидины; поликонденсация; дезинфицирующие средства; антимикробное действие; резистентные штаммы
The study was financially supported by the state assignment of the Baikal Institute of Nature Management SB RAS.
ru
The resistance of bacteria to the disinfectants used is one of the pressing health problems requiring to be addressed in order to prevent formation and spread of resistant strains. Bacterial resistance leads to a sharply decreased effectiveness of anti-epidemic measures and contributes to maintaining a high morbidity level. In the context of the growing HAI incidence rate and related polyetiology, the large adaptive potential of opportunistic microorganisms, and the growing resistance to antimicrobial drugs, it is necessary to search for new or modify the corresponding substances of plant or synthetic origin exerting antimicrobial action used as antimicrobial agents. One of the representatives of this class of compounds are polyguanidines, characterized by high antimicrobial activity and low toxicity. Due to the high reactivity of the guanidine group, as well as ease of synthesis and relative availability of raw materials, N-phenyl- and N-octyl-substituted derivatives of polyhexamethylene guanidine hydrochloride were able to be obtained by melt polycondensation, their molecular weight characteristics were measured as well as the structure investigated by IR spectroscopy. An earlier study on the acute toxicity for polyhexamethylene guanidine hydrochloride derivatives after a single oral drug intake in laboratory animals (white mice) allowed to establish the following LD50 values: polyhexamethylene guanidine hydrochloride — 850.0±112.02 mg/ kg; N-phenyl-substituted polyhexamethylene guanidine hydrochloride — 1399.9±120.51 mg/kg; N-octyl-substituted polyhexamethylene guanidine hydrochloride — 1150.0±137.40 mg/kg. Such values, according to the tabulation of hazard classes, allow the synthesized derivatives to be classified into the fourth hazard class and open up an opportunity for using disinfectants as active components. The evaluation of the antibacterial sample properties was carried out by using serial dilutions in agar on hospital bacterial and fungal strains isolated from the biomaterial of patients at the Republican Clinical Hospital named after V.I. Semashko, according to the clinical guidelines “Laboratory diagnosis of community-acquired pneumonia” 2014, “Bacteriological analysis of urine” 2014, “Determination of the susceptibility of microorganisms to antimicrobial drugs” 2015. It was found that N-substituted derivatives exhibit a greater antimicrobial effect compared with unsubstituted polymer. The most sensitive to all the drugs presented was the yeast-like fungi Candida albicans (No. 2495) (complete suppression), as well as methicillin-resistant S. aureus (no. 2544), and the substituted samples almost completely suppress its growth. The most resistant strains are P. aeruginosa (No. 2281), A. baumannii (No. 2806) and K. pneumoniae (No. 3023), the percentage of reduction of these bacteria under the action of substituted samples does not exceed 41%, which is accounted for by their multi-resistance.
oai:iimmun.ru:article/2086
2023-10-24T12:02:01Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/2086
2023-10-24T12:02:01Z
Russian Journal of Infection and Immunity
Vol 13, No 3 (2023); 573-578
Adhesiveness of the specific phages to yersiniae cells assessed by optical trapping
https://iimmun.ru/iimm/article/download/2086/15096
https://iimmun.ru/iimm/article/download/2086/15097
https://iimmun.ru/iimm/article/download/2086/15098
https://iimmun.ru/iimm/article/download/2086/15099
https://iimmun.ru/iimm/article/download/2086/15100
https://iimmun.ru/iimm/article/download/2086/15101
https://iimmun.ru/iimm/article/download/2086/15102
https://iimmun.ru/iimm/article/download/2086/15103
https://iimmun.ru/iimm/article/download/2086/15104
https://iimmun.ru/iimm/article/download/2086/15105
https://iimmun.ru/iimm/article/download/2086/15144
https://iimmun.ru/iimm/article/download/2086/15145
https://iimmun.ru/iimm/article/download/2086/15146
https://iimmun.ru/iimm/article/download/2086/15147
https://iimmun.ru/iimm/article/download/2086/15148
https://iimmun.ru/iimm/article/download/2086/119955
https://iimmun.ru/iimm/article/download/2086/119956
https://iimmun.ru/iimm/article/download/2086/119957
https://iimmun.ru/iimm/article/download/2086/119961
https://iimmun.ru/iimm/article/download/2086/123650
https://iimmun.ru/iimm/article/download/2086/139475
https://iimmun.ru/iimm/article/download/2086/139476
Konyshev I.V.; Institute of Physiology of the Komi Scientific Center of the Ural Branch of the Russian Academy of Sciences; Vyatka State University
Dudina L.G.; Institute of Physiology of the Komi Scientific Center of the Ural Branch of the Russian Academy of Sciences; Vyatka State University
Morozova N.A.; Vyatka State University
Byvalov A.A.; Institute of Physiology of the Komi Scientific Center of the Ural Branch of the Russian Academy of Sciences; Vyatka State University
2023-06-26 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
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Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/2086
бактериофаг; Yersinia pseudotuberculosis; Yersinia pestis; сенсибилизация; лазерная ловушка
Array
1021051201894-0
ru
In recent years, there has been increasing interest in using bacteriophages in the complex therapy of some infectious diseases. Objectively, it is related to the spread of antibiotic resistance and a small number of side effects triggered by phages applied locally and parenterally. Yersiniae are no exception to this rule, but their interaction with virions has not been studied thoroughly. In three types of lytic Yersiniae bacteriophages are used for diagnostic purposes, which differ in specificity, virulence, and morphological features. We evaluated the interaction force between phages and bacteriae by optical trapping.
Materials and methods. Live Yersinia pseudotuberculosis and Y. pestis cells with different LPS structure and preparations of three bacteriophages — Pokrovskaya’s phage, d’Herelle’s phage (also named as the pseudotuberculosis phage) and the phage L-413C were used. The Pokrovskaya’s phage is well studied and widely used in diagnostics, but the mechanism of its adsorption on Y. pestis and Y. pseudotuberculosis cells is not well-understood. The phage L-413C lyses plague bacteria, while d’Herrel’s phage can lyse both pathogens. Virions were linked to the surface of aminated glass. Optically trapped microbial cells were approached to the glass surface and then retracted in the opposite direction. The amplitude of the leap was recalculated into force through calibration coefficients. The primary data were processed in the program “R”; statistical analysis was performed using “Matlab 7.0” and “Statistica 12”
Results and discussion. The analysis of histograms revealed the presence of a specific component in the «Y. pseudotuberculosis–d’Herrell’s phage» (Fmean = 7.46±3.52 pN) system, as well as in all pairs involving plague microbe. In the latter case, the interaction forces were comparable: 8.64±3.83 pN for d’Herelle’s phage,11.03±4.22 pN for Pokrovskaya’s phage, and 10.42±4.79 pN for phage L-413C. The average detachment force from the substrate treated with bovine serum albumin (BSA) was comparable for both cell types. Our results convincingly prove an opportunity for using the optical trapping to estimate interaction force in other «bacterium–phage» systems.
oai:iimmun.ru:article/705
2022-04-12T15:46:10Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/705
2022-04-12T15:46:10Z
Russian Journal of Infection and Immunity
Vol 9, No 2 (2019); 363-368
Bioregulation of amplitude-phase biological activity of Candida albicans by women reproductive tract microsymbionts
Nikolenko M.V.; Tyumen State Medical University
Baryshnikova N.V.; Tyumen State Medical University
Bakhlykova E.A.; Tyumen State Medical University
Zykova A.V.; Tyumen State Medical University
Romanchenko K.V.; Tyumen State Medical University
2019-05-13 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/705
Candida albicans; микросимбионты; дисбиоз; мезор, акрофаза; амплитудно-фазовые характеристики
ru
In this study, we propose a chronobiological method for examining inter-microbial interactions in bacterial and fungal associations in female reproductive tract. Fungal and bacterial species were isolated in 45 women of reproductive aged 19–35, with regular menstrual cycle, applying no hormonal contraceptives, without previous gynecological surgery, abortions, miscarriages with vaginal eubiosis and dysbiosis in history. Sexually transmitted diseases (HIV infection, syphilis, gonorrhea, trichomoniasis, chlamydiosis) were excluded in all subjects. Proliferation rate, morphogenesis and phospholipase activity were examined within the 48-hour period every 4 hours, in winter time, Moon phase IV. The data obtained were assessed by using Student’s t-test, Wilcoxon test, and least squares method. All subjects were divided into the groups: group 1 — women with vaginal eubiosis, group 2 — women with vaginal dysbiosis. It was shown that in all subjects experimental parameters of C. albicans cultures showed a diurnal dynamics characterized in healthy women by circadian rhythms with a single peak of activity. However, in women with vaginal dysbiosis C. albicans was characterized by significant ultradian (around 12 hours long) rhythms with two peaks of biological activity. Concurrence and consistency in manifested physiological functions related to clinical isolates was coupled to temporal pattern of distributed biological resources in fungi depending on course of infectious process. It was found that in vaginal eubiosis exometabolites released by dominant associated microbiota did not significantly change microbiota-related amplitude-phase parameters. The data obtained evidenced that temporal pattern of parameters related to C. albicans from healthy individuals was stable and independent on bacterial metabolites. In contrast, dominant microsymbiont in vaginal dysbiosis inhibited fungi-related rhythms, which might be important in establishing lactobacillus-associated biotope colonization resistance. Effects of metabolites released by the associated microbiota typical to dysbiosis was revealed by increased mesor, amplitude, preserved biorhythm spectral pattern in examined properties as well as amplitude-phase characteristics indicating at enhanced or sustained C. albicans adaptive potential. Therefore, the amplitude-phase parameter of C. albicans physiological activity served as a marker of opposite (enhanced/weakened) effect of microsymbiont survival described in “microbial dominant-associate” pairs.
oai:iimmun.ru:article/1357
2023-08-06T17:10:31Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/1357
2023-08-06T17:10:31Z
Russian Journal of Infection and Immunity
Vol 10, No 4 (2020); 762-768
Assessing cytokine status of patients with chronic endometritis combined with endometrial hyperplastic processes in reproductive period
Tkachenko L.V.; Volgograd State Medical University
Sviridova N.I.; Volgograd State Medical University
Zharkin N.A.; Volgograd State Medical University
Burova N.A.; Volgograd State Medical University
Belan E.B.; Volgograd State Medical University
2020-11-26 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1357
цитокиновый статус; хронический эндометрит; гиперпластические процессы эндометрия; репродуктивный период
ru
The goal of our study was to examine local and serum cytokine level involved in regulating inflammation in patients with chronic endometritis combined with endometrial hyperplastic processes. On admission, all patients underwent hysteroscopy with separate diagnostic curettage followed by histological examination of samples isolated from the uterine and cervical canal mucosa. Such manipulations were indicated due to abnormal uterine bleeding as well as suspected endometrial pathology based on ultrasound examination. According to the histological examination data of the endometrial samples, all patients were divided into two groups: group I contained 45 women with CE combined with PEG without atypia; group II — 38 patients with morphologically verified CEE combined with AEG without atypia. Level of IL-1P, IL-2, IL-6, IFNy, TNFa in biological fluids (aspirate from the uterus; serum) was measured by using enzyme-linked immunosorbent assay. We found that in both groups (91.1% and 89.6%, respectively) the vast majority of patients was hospitalized due to abnormal uterine bleeding. Oligomenorrhea alternated with intermenstrual bleeding (66.7% and 71.2%, respectively) and dominated in pattern of menstrual cycle disorders in the examined patients, whereas 11 (24.4%) and 7 (18.4%) patients from group I and II, respectively, were noted to suffer from severe menstrual bleeding. Overall, analyzing the data on cytokine level both in the uterine aspirate and serum evidences about ongoing inflammatory process found at examination time point. Upon that, such process was not only local, but also exhibited signs of a systemic inflammatory response. The data on cytokine level in the uterine aspirate from patients with CE coupled to PGE or CGE without atypia point at local inflammatory process characterized by significantly increased concentration of IL-ф, IL-2, IL-6, TNFa and IFNy. At the same time, higher level of IL-ф and IFNy in patients from group II might indicates that degree of morphological changes in the endometrium could affect the level of local cytokine production. Thus, the data obtained evidence that immune changes in chronic endometritis combined with non-atypical endometrial hyperplastic processes mostly occur locally. In this regard, measuring cytokine concentration in the uterine aspirate is a diagnostic predictor and serves as a sign for monitoring therapeutic effectiveness of therapy in this cohort of patients.
oai:oai.iimmun.ru:article/1569
2020-08-07T07:04:12Z
iimm:SHORT
oai:iimmun.ru:article/156
2015-06-16T09:40:30Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/156
2015-06-16T09:40:30Z
Russian Journal of Infection and Immunity
Vol 4, No 1 (2014); 43-48
THE SPECTRUM OF BACTEREMIA PATHOGENS IN PATIENTS WITH IMMUNE DEFICIENCY STATES OF VARIOUS ORIGINS
Polukhina O.V.; Federal State Budgetary Institution «Russian Scientific Center of Radiology and Surgical Technologies», Ministry of Health of the Russian Federation, St. Petersburg
Suborova T.N.; Federal State Budgetary Military Educational Institution of Higher Professional Education “Kirov Military Medical Academy”, Ministry of Defense of the Russian Federation, St. Petersburg
Kuzin A.A.; Federal State Budgetary Military Educational Institution of Higher Professional Education “Kirov Military Medical Academy”, Ministry of Defense of the Russian Federation, St. Petersburg
Petrov A.N.; Federal State Budgetary Military Educational Institution of Higher Professional Education “Kirov Military Medical Academy”, Ministry of Defense of the Russian Federation, St. Petersburg
Osovskikh V.V.; Federal State Budgetary Institution «Russian Scientific Center of Radiology and Surgical Technologies», Ministry of Health of the Russian Federation, St. Petersburg
Granov D.A.; Federal State Budgetary Institution «Russian Scientific Center of Radiology and Surgical Technologies», Ministry of Health of the Russian Federation, St. Petersburg
Pilipenko V.V.; Federal State Budgetary Institution «Russian Scientific Center of Radiology and Surgical Technologies», Ministry of Health of the Russian Federation, St. Petersburg
2014-07-09 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/156
онкоурологические заболевания; трансплантация; ранения; травмы; бактериемия; возбудители
ru
Abstract. A comparative analysis of the results of bacteriological study of blood samples obtained from 1608 patients with secondary immunodeficiency of different origins has been conducted. It was established that within the spectrum of pathogens of bacteremia in these onco-urological profile patients, enterobacteria was the predominant one. In post–liver transplantation patients micromycete was predominant, and for wounded patients and victims with serious injuries staphylococci were more often isolated.
oai:iimmun.ru:article/1465
2023-08-06T17:10:13Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/1465
2023-08-06T17:10:13Z
Russian Journal of Infection and Immunity
Vol 11, No 2 (2021); 383-388
Features of proinflammatory cytokine production in patients with allergic rhinitis combined with <i>Helicobacter pylori</i>-associated pathologies
https://iimmun.ru/iimm/article/download/1465/8520
https://iimmun.ru/iimm/article/download/1465/8521
https://iimmun.ru/iimm/article/download/1465/8522
https://iimmun.ru/iimm/article/download/1465/8523
https://iimmun.ru/iimm/article/download/1465/8524
https://iimmun.ru/iimm/article/download/1465/8525
https://iimmun.ru/iimm/article/download/1465/8526
https://iimmun.ru/iimm/article/download/1465/9951
Iraklionova N.S.; Volgograd State Medical University
Belan E.B.; Volgograd State Medical University
Turkina S.V.; Volgograd State Medical University
Dotsenko A.M.; Volgograd State Medical University
2020-08-25 17:15:27
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1465
желудочно-кишечный тракт; Helicobacter pylori; цитокины; IL-6; IL-8; воспаление; аллергический ринит
Авторы выражают благодарность руководству ГБУЗ Волгоградская областная клиническая больница № 1 и ГУЗ Консультативно-диагностическая поликлиника № 2 (г. Волгоград) за оказанную помощь при проведении данного исследования.
ru
Investigating a role for pro- and anti-inflammatory cytokines in immune and inflammatory response represents one of the most pressing topics in current immunology. There have been accumulated a great body of data regarding the cytokine status during inflammatory processes in gastrointestinal tract. However, many issues remain poorly elucidated. Moreover, most of the studies were mainly aimed at examining biopsy material cytokines collected from various parts of the gastrointestinal tract or gastric juice. Here we present the data on investigating the features of cytokine production in patients with allergic rhinitis combined with H. pylori-associated diseases of the upper gastrointestinal tract. A total of 225 patients aged 18 to 40 years (mean age 29.5±6.74 years) were examined, among which 43 patients suffered from allergic rhinitis, 66 patients had inflammatory diseases of the upper gastrointestinal tract (chronic gastritis/gastroduodenitis), and 69 patients — allergic rhinitis with concomitant inflammatory diseases of the upper gastrointestinal tract. Patients were stratified to various study groups depending on diagnosed allergic rhinitis and H. pylori infection. Serum IL-6 and IL-8 level and complete blood cell count were measured. Statistical significance for difference in parameter frequencies between independent groups was assessed by using Fisher's exact test or χ2 criteria. It was shown that serum IL-6 level in H. pylori-negative patients was within the reference interval with single spikes, whereas its production was markedly increased in patients with H. pylori infection regardless of allergic pathology. Frequency of subjects with elevated serum IL-8 level was comparable between groups of patients with gastrointestinal diseases, but significantly higher than that one in the control group. An interdependence between serum IL-8 level and white blood cell count was found, which is of interest for predicting development of the inflammatory process. Thus, the serum IL-8 level during the upper gastrointestinal tract inflammatory diseases was increased regardless of verified of H. pylori infection and allergic rhinitis, whereas increased IL-6 serum level was observed solely in H. pylori-positive patients.
oai:iimmun.ru:article/1904
2023-01-17T13:08:33Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/1904
2023-01-17T13:08:33Z
Russian Journal of Infection and Immunity
Vol 12, No 4 (2022); 771-778
Characterizing A “N-CoV-2-IgG PS” diagnostic kit to quantify SARS-CoV-2 nucleocapsid protein-specific human IgG antibodies
https://iimmun.ru/iimm/article/download/1904/12887
https://iimmun.ru/iimm/article/download/1904/12888
https://iimmun.ru/iimm/article/download/1904/12889
https://iimmun.ru/iimm/article/download/1904/12890
https://iimmun.ru/iimm/article/download/1904/12891
https://iimmun.ru/iimm/article/download/1904/12892
https://iimmun.ru/iimm/article/download/1904/12893
https://iimmun.ru/iimm/article/download/1904/12894
https://iimmun.ru/iimm/article/download/1904/12895
https://iimmun.ru/iimm/article/download/1904/14154
https://iimmun.ru/iimm/article/download/1904/14373
https://iimmun.ru/iimm/article/download/1904/14374
https://iimmun.ru/iimm/article/download/1904/14375
Zueva E.V.; St. Petersburg Pasteur Institute
Belyaev N.N.; St. Petersburg Pasteur Institute
Verbov V.N.; St. Petersburg Pasteur Institute
Likhachev I.V.; St. Petersburg Pasteur Institute
Bachinin I.A.; St. Petersburg Pasteur Institute
Khamitova I.V.; St. Petersburg Pasteur Institute
Korobova Z.R.; St. Petersburg Pasteur Institute; Pavlov First St. Petersburg State Medical University
Arsentieva N.A.; St. Petersburg Pasteur Institute
Totolian A. .; St. Petersburg Pasteur Institute; Pavlov First St. Petersburg State Medical University
2022-11-15 12:49:19
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1904
COVID-19; ИФА-наборы; N-белок SARS-CoV-2; единицы BAU/мл; N-белок-связывающие антитела; нейтрализующие антитела; анализ антител
Array
ru
Confirming detected SARS-CoV-2-specific antibodies is necessary to reveal immune response in COVID-19 convalescent subjects as well as to conduct population studies by screening for specific antibodies to assess rate of COVID-19 prevalence. With this purpose St. Petersburg Pasteur Institute was the first in Russia to develop the ELISA kit for the quantitative determination of human IgG to the SARS-CoV-2 nucleocapsid (N-CoV-2-IgG PS). Arbitrary units (AU/ml) were used to assess the level of antibodies. The data shown in AU/ml were recalculated later to the international units (BAU/ml) in accordance with established the First WHO International Standard for anti-SARS-CoV-2 human Immunoglobulin. Comparing the data of the N-CoV-2-IgG PS calibration curve with those of the First WHO International Standard for anti-SARS-CoV-2 human Immunoglobulin revealed a complete inter-assay association (r = 0.999, R2 = 0.997) allowing to find that 1BAU/ml = 5.97 AU/ml. The aim of the study was to characterize the “SARS-CoV-2 protein N Human IgG Quantitative ELISA Kit” (N-CoV-2-IgG PS), compare quantitative and qualitative data of ELISA kits, assess a correlation between the binding antibodies to SARS-CoV-2 N proteins and the neutralizing antibodies against SARS-CoV-2. The data of correlation analysis of the 83 COVID-19 convalescent blood plasma samples a significant relationship between the antibodies quantitative values and titers SARS-CoV-2-specific antibody (r = 0.8436, R2 = 0.7802) as well as a moderate relationship between antibody concentration and positivity index (r = 0.6648, R2 = 0.3307), assessed by Chaddock scale. Comparing concentration of N-protein binding antibodies with neutralizing antibody titers level uncovered data consistency obtained by quantitative and virus microneutralization assays (r = 0.7310, R2 = 0.6527) used in parallel to analyze 80 blood plasma samples obtained from COVID-19 patients and convalescents. AUC under the ROC curve comprised 0.701 (P < 0.0001) evidencing about a satisfactory informative value for “N-CoV-2-IgG PS” compared with microneutralization assay. In addition, the efficacy of the “N-CoV-2-IgG PS” was 95%, while the positive and negative prognostic value was 97% and 87%, respectively. The data obtained confirmed a correlation between N-protein binding antibody level and neutralizing antibody titer. Checking inter-assay agreement evidenced about acceptance for informativeness and efficacy of using “N-CoV-2-IgG PS”, thereby confirming an opportunity to apply the Kit to screen for SARS-CoV-2 N protein-specific IgG antibody level and assess seroprevalence in diverse population cohorts.
oai:iimmun.ru:article/562
2022-04-12T15:20:39Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/562
2022-04-12T15:20:39Z
Russian Journal of Infection and Immunity
Vol 7, No 3 (2017); 292-296
COMPARISON OF INFLUENZA A(H1N1)pdm09 GENOMES OBTAINED FROM DIFFERENT TYPES OF VIRUS-CONTAINING MATERIAL
Fadeev A.V.; Research Institute of Influenza
Zhilinskaya I.N.; Research Institute of Influenza
2017-09-30 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/562
вирус гриппа А; полногеномное секвенирование; эпидемиологический надзор; гемагглютинин; адаптационные мутации; рецепторная специфичность
ru
This paper describes results of comparison of genetic sequences of inf luenza viruses obtained from clinical samples (nasopharyngeal swabs) and viruses isolated on MDCK cells and in developing chick embryos. The obtained data shows that 1–2x fold passaging of inf luenza viruses in MDCK cells does not lead to additional mutations in the genome of the inf luenza virus, and the genetic material of this virus is identical to the genetic material from the clinical sample. When passaging viruses in chick embryos within 1–2 passages, the genetic material of the virus differs from the genetic material from the clinical sample, because of mutations in the receptor region of hemagglutinin, leading to aminoacid substitution Q223R. The mutation Q223R changes the receptor specificity of hemagglutinin from human-type receptors (α-2.6-sialic acids) to avian receptors (α-2.3-sialic acids). The data obtained coincide with the data of Japanese researchers who showed that a similar substitution in the hemagglutinin gene was detected in Japanese strains of inf luenza virus subtype A (H1N1) pdm09, passaged in chick embryos for 1–2 passages. With their further passaging in chick embryos, the number of acquired mutations in the surface proteins of these viruses increases. Thus, we have shown that for the genetic analysis of epidemic-relevant strains of inf luenza viruses for routine epidemiological surveillance tasks, the use of direct sequencing of clinical samples containing the genetic material of viruses makes it possible to obtain the most reliable data, to increase their efficiency, since it saves time normally spent on virus isolation procedure. If preliminary isolation of viruses is necessary, for example, if it is not possible to carry out full genome amplification from a clinical sample (low content of the material, presence of polymerase chain reaction inhibitors), virus isolation on MDCK cells with a minimal amount of passage is optimal, which is used in practice of epidemiological surveillance.
oai:iimmun.ru:article/896
2023-08-06T17:12:01Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/896
2023-08-06T17:12:01Z
Russian Journal of Infection and Immunity
Vol 9, No 5-6 (2019); 811-816
Abundance of <i>Ixodes persulcatus</i> ticks in Komi Republic as a function of an air temperature
https://iimmun.ru/iimm/article/download/896/4723
https://iimmun.ru/iimm/article/download/896/4724
https://iimmun.ru/iimm/article/download/896/4725
https://iimmun.ru/iimm/article/download/896/4726
https://iimmun.ru/iimm/article/download/896/4727
https://iimmun.ru/iimm/article/download/896/4728
https://iimmun.ru/iimm/article/download/896/4822
https://iimmun.ru/iimm/article/download/896/6694
https://iimmun.ru/iimm/article/download/896/6696
https://iimmun.ru/iimm/article/download/896/6695
https://iimmun.ru/iimm/article/download/896/6697
https://iimmun.ru/iimm/article/download/896/6698
https://iimmun.ru/iimm/article/download/896/6699
https://iimmun.ru/iimm/article/download/896/6700
Tronin A.A.; Scientific Research Center for Ecological Safety, Russian Academy of Sciences
Tokarevich N.K.; St. Petersburg Pasteur Institute
Gnativ B.R.; Center for Hygiene and Epidemiology of Komi Republic
2020-01-31 21:03:39
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/896
клещевой энцефалит; нападение клещей; Ixodes persulcatus; популяция; изменение климата; температура воздуха; Республика Коми
en
The sharp rising incidence of tick-borne encephalitis (TBE) in Komi Republic at the North-east of European Russia was recorded last decades. Tick-bite incidence also was grown. Rapid rise of TBE incidence growth and Ixodidae ticks depends on a number of factors, and the impact of climate change being one of them. Ixodes persulcatus ticks is considered as a main vector of TBE in Komi. Our objective is to estimate the influence of air temperature change on the tickbite incidence and Ixodes persulcatus population in Komi. Komi Republic is located near the Polar circle where the northern frontier of Ixodes persulcatus ticks situated and we expected the growth of tick’s population. The number of Komi inhabitants seeking medical care after tick bites in 1992–2014 was considered. Gridded monthly air temperature data with grid size 0.5 degree were recalculated to temperature referred to Komi administrative units. The time series of annual number of tick victims from 1992 till 2014 and model air temperature from 1948 till 2016 for all Komi administrative units were compiled. We analyzed the data on tick-bite incidence in Komi administrative units in relation to changes in local annual average air temperature within the study area. The linear dependence of the tick-bite incidence on air temperature was established when of the tick-bite incidence is represented in logarithm form. The tick population depends not only on temperature but humidity, landcover and hosts. Described areas of Komi belong to humid climate, where precipitations exceed evaporation. Most of the Komi territory is covered by taiga with underwood, grass and bush. Hosts of the first and the major levels are represented by birds and rodents. The dependence of tick-bite incidence and temperature looks like “Malthus’s law”, but the development of population depends on temperature not on time. The exponential growth in the nearest future will ceased and the population will proceed to stable phase. Ticks population in Komi Republic is moving to the North and the air temperature determines the dynamics of population.
oai:iimmun.ru:article/46
2022-04-12T21:19:45Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/46
2022-04-12T21:19:45Z
Russian Journal of Infection and Immunity
Vol 1, No 2 (2011); 185-188
SIGNIFICANCE OF UROGENITAL INFECTIONS IN PATHOLOGY OF PREGNANCY AND DELIVERY IN EXPERIMENTS
Arshba I.M.; ГУН НИИ медицинской приматологии РАМН, г. Сочи
Dzhikidze E.K.; ГУН НИИ медицинской приматологии РАМН, г. Сочи
2014-06-28 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/46
обезьяны; урогенитальная инфекция; хламидии; микоплазмы; уреаплазмы
ru
Abstract. Development of adequate models in laboratory animals is highly important to detect etiological links between urogenital tract pathology and chlamydia, mycoplasma and ureaplasma bacteria. These model systems can be very useful to find solutions in combating with urogenital tract diseases. One of the obligatory conditions to use monkeys in development of experimental models of infectious and somatic human diseases is knowledge of spontaneous pathology in primates. The present study has shown high frequency of urogenital infections bacteria in the urogenital tract of healthy and sick monkeys.
oai:iimmun.ru:article/279
2022-04-13T15:15:26Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/279
2022-04-13T15:15:26Z
Russian Journal of Infection and Immunity
Vol 5, No 1 (2015); 89-92
DURATION OF POSTVACCINAL IMMUNITY AGAINST HEPATITIS B IN HEALTH CARE WORKERS IN ST. PETERSBURG
Kalinina Z.P.; 198095, Russian Federation, St. Petersburg, str. Shkapina, 30, letter A, St. Petersburg Мedical Informational-Analytical Center.
North-Western State Medical University named after I.I. Mechnikov, St. Petersburg, Russian Federation
Dar’ina M.G.; 198095, Russian Federation, St. Petersburg, str. Shkapina, 30, letter A, St. Petersburg Мedical Informational-Analytical Center.
North-Western State Medical University named after I.I. Mechnikov, St. Petersburg, Russian Federation
Movchan K.N.; 198095, Russian Federation, St. Petersburg, str. Shkapina, 30, letter A, St. Petersburg Мedical Informational-Analytical Center.
North-Western State Medical University named after I.I. Mechnikov, St. Petersburg, Russian Federation
Mamicheva O.Y.; North-Western State Medical University named after I.I. Mechnikov, St. Petersburg, Russian Federation
Gagarkina I.B.; 198095, Russian Federation, St. Petersburg, str. Shkapina, 30, letter A, St. Petersburg Мedical Informational-Analytical Center.
Averina T.Y.; 198095, Russian Federation, St. Petersburg, str. Shkapina, 30, letter A, St. Petersburg Мedical Informational-Analytical Center.
Balabash O.A.; 198095, Russian Federation, St. Petersburg, str. Shkapina, 30, letter A, St. Petersburg Мedical Informational-Analytical Center.
Yefimova L.I.; 198095, Russian Federation, St. Petersburg, str. Shkapina, 30, letter A, St. Petersburg Мedical Informational-Analytical Center.
Potemkina I.V.; 198095, Russian Federation, St. Petersburg, str. Shkapina, 30, letter A, St. Petersburg Мedical Informational-Analytical Center.
2015-04-21 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/279
вирусный гепатит В;иммунизация;профилактика;медицинские работники;анти-HBsAg.
ru
Immunization against hepatitis В is the most effective method of preventing this disease. Study of quantitative characteristics of postvaccinal immunity against viral hepatitis В in 214 health care workers from different institutions of St. Petersburg has shown that HBV vaccine provides a long term immunity in 80% of cases with the absence of manifest forms of the disease. The duration of circulation of high titers of antibodies depended on the age of people when vaccination was provided. The highest level of immunity was observed in people vaccinated before the age of 30 years (84,0–90,6%). Reduction of immunity level in vaccinated health care workers after 5 years from the date of vaccination requires revaccination against viral hepatitis B after pre-vaccination screening.
oai:iimmun.ru:article/1614
2023-08-06T17:09:43Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/1614
2023-08-06T17:09:43Z
Russian Journal of Infection and Immunity
Vol 11, No 5 (2021); 979-983
Profile of several autoantibodies, complement and vitamin D levels in Brazilian patients with Chikungunya infection
https://iimmun.ru/iimm/article/download/1614/11398
Carvalho J.F.; Federal University of Bahia
Cordeiro L.R.; Federal University of Bahia
Silva F.F.; University of São Paulo
Mota L.; University of Brasilia
Rodrigues C.; University of Fortaleza
Andrade C.F.; National School of Public Health Sérgio Arouca; Vassouras University
Caldas C.; Federal University of Pará
2021-07-14 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1614
Чикунгунья; аутоантитела; аутоиммунитет; арбовироз; ревматоидный артрит
en
Introduction. Chikungunya virus infection (CKV) may lead to chronic arthritis in up to 40% of the patients. There are previous data regarding positive auto antibodies in CKV. Objective is to systematically evaluate the prevalence of auto antibodies in CKV patients. Methods. All study participants had clinical manifestations being CKV positive at least serologally or by RT-PCR data. The following autoantibodies were assessed: antinuclear antibodies (ANA), anti-dsDNA, anti-Sm, anti-Ro/SS-A, anti-La/SS-B, anti-U1RNP, IgG and IgM anticardiolipin, anticyclic citrullinated peptide (antiCCP), rheumatoid factor (RF), antiribosomal P protein, lupus anticoagulant, anti-Jo-1 and anti-Scl-70. CH100, C3 and C4 complement components, serum levels of immunoglobulins, C-reactive protein, erythrocyte sedimentation rate, alpha1-acid glycoprotein, antithyroglobulin, antithyroperoxidase, TRAb, 25 hydroxyvitamin D (25OHD), dengue and zika IgG and IgM antibodies were also measured. Results. 30 CKV patients were included. Mean age was 59.1±15.7 years, 85% females and 77% Caucasian subjects. Disease duration comprised 4.9±4.0 months. Oligoarthritis was observed in 93% cases. Mean C-reactive protein levels were 10.1±6.8 ng/dL, erythrocyte sedimentation rate — 34.2±19.9 mm/1st hour and alpha1-acid glycoprotein 115.5±52.6 mg/dL. Intramuscular betamethasone depot single dose injection was administered in 54%, prednisone — in 15% and methotrexate — in 23% cases. Importantly, 1/30 (3.3%) cases was positive for ANA, 4/30 (13.3%) — for RF and none was positive for anti-CCP or any other autoantibodies. Complement and immunoglobulin levels were all within the normal range. Low levels of 25OHD were observed in 88% cases.
oai:iimmun.ru:article/1613
2023-04-01T17:56:25Z
iimm:SHORT
v2
https://iimmun.ru/iimm/article/view/1613
2023-04-01T17:56:25Z
Russian Journal of Infection and Immunity
Vol 13, No 1 (2023); 171-173
RAS and SARS-CoV-2 interaction: short review of the latest evidence
https://iimmun.ru/iimm/article/download/1613/9853
https://iimmun.ru/iimm/article/download/1613/9854
https://iimmun.ru/iimm/article/download/1613/11759
https://iimmun.ru/iimm/article/download/1613/74123
Vitiello A.; Ministry of Health
Ferrara F.; Asl Napoli 3 Sud
2023-04-01 18:41:52
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1613
COVID-19; ренин-ангиотензиновая система; SARS-CoV-2; пандемия
Array
en
Coronavirus SARS-CoV-2 is responsible for the coronavirus disease (COVID-19) cause of the recent global pandemic, which is causing thousands of deaths worldwide and represents a health challenge with few precedents in human history. The angiotensin 2 conversion enzyme (ACE-2) has been identified as the receptor that facilitates access to SARS-CoV-2 in cells; evidence shows that its concentration varies during the various stages of viral infection. Therapeutic agents modifying the renin-angiotensin system (RAS) may be able to modulate the concentration of ACE-2 and the various components of the system. In this article we examine the latest evidence on the association between the use of RAS modifying agents and coronavirus 2019 (COVID-19) disease caused by SARS-CoV-2. Our investigation and critical literature research does not suggest discontinuation of ACEIs/ARBs treatment in clinical practice as there is a lack of robust evidence. However, we recommend further well-structured epidemiological studies investigating this sensitive issue that may provide important new suggestions for implementing guidelines.
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