2024-03-28T20:57:50Z
https://iimmun.ru/index/oai
oai:iimmun.ru:article/633
2023-08-06T17:11:37Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/633
2023-08-06T17:11:37Z
Russian Journal of Infection and Immunity
Vol 9, No 5-6 (2019); 779-786
Surveying children and adult vaccination program against diphtheria, tetanus, measles and viral hepatitis B in the Russian Federation
https://iimmun.ru/iimm/article/download/633/3202
https://iimmun.ru/iimm/article/download/633/3203
https://iimmun.ru/iimm/article/download/633/3204
https://iimmun.ru/iimm/article/download/633/3205
https://iimmun.ru/iimm/article/download/633/3206
https://iimmun.ru/iimm/article/download/633/3207
https://iimmun.ru/iimm/article/download/633/3208
https://iimmun.ru/iimm/article/download/633/3209
https://iimmun.ru/iimm/article/download/633/3210
https://iimmun.ru/iimm/article/download/633/3211
https://iimmun.ru/iimm/article/download/633/3212
https://iimmun.ru/iimm/article/download/633/3213
https://iimmun.ru/iimm/article/download/633/5964
https://iimmun.ru/iimm/article/download/633/5965
https://iimmun.ru/iimm/article/download/633/5966
https://iimmun.ru/iimm/article/download/633/5967
https://iimmun.ru/iimm/article/download/633/5968
https://iimmun.ru/iimm/article/download/633/5969
https://iimmun.ru/iimm/article/download/633/5970
https://iimmun.ru/iimm/article/download/633/5971
https://iimmun.ru/iimm/article/download/633/5972
https://iimmun.ru/iimm/article/download/633/5973
https://iimmun.ru/iimm/article/download/633/5974
Galina N.P.; Sechenov First Moscow State Medical University
Mindlina A.Y.; Sechenov First Moscow State Medical University
Polibin R.V.; Sechenov First Moscow State Medical University
2019-12-04 19:51:30
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/633
вакцинация; иммунизация; своевременность вакцинации; охват прививками; вакциноуправляемые инфекции; корь; дифтерия; столбняк; гепатит В; коклюш
ru
Over the last years, incidence of vaccine-preventable infections tended to increase both in Russia and multiple European countries due to diverse reasons, including loss of alertness in medical workers to ensure proper quality of immunization. Currently, vaccination is considered to be the most efficient means for preventing infectious diseases. However, vaccination efficacy is directly related to population coverage and proper timeframe for its application.Materials and methods. Diphtheria, tetanus, measles and hepatitis B vaccination coverage and timeframe were analyzed for population of Russia, Moscow as well as the three pediatric and adult out-patient hospitals.Results. It was found that compared to adults, pediatric subjects were vaccinated better. However, in case of 95% or higher coverage against all infections, shortcomings in vaccination timeframe were uncovered. In 2017, despite pediatric subjects should be vaccinated against diphtheria and tetanus at the age of 3-to-6 months, coverage was as low as 46.9% in Russia, 48.7% in Moscow that ranged in some polyclinics from 21.8% in 2012 down to 1.7% in 2017. Moreover, this situation was even more unfavorable in case of revaccination program. In particular, it was found that at the age of 18 months only 50, 54, and 49–55% underwent revaccination in the Russia, Moscow 54%, and some out-patient hospitals, whereas at the age of 7–14 years in Russia there were revaccinated up to 97% population. Likewise, at the age of 6 months there were covered with vaccination against hepatitis B as few as 49% population in Russia and Moscow, whereas more than 90% coverage was achieved only at the age of 1 year. Immunization coverage reaching more than 85% of adult population against hepatitis B is superior to diphtheria and tetanus. Immunization coverage against measles in Russia at all age groups was over 90% population. Thus, it is necessary to optimize activities on adhering to vaccination timeframe, as decline in quality of vaccination inevitably leads to elevated incidence rate of vaccine-preventable infections. This and vaccination coverage might be adjusted by introducing a Preventive Vaccination Surveillance and Reporting Automated System in the Russian Federation.
oai:iimmun.ru:article/1179
2022-04-12T15:51:23Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/1179
2022-04-12T15:51:23Z
Russian Journal of Infection and Immunity
Vol 10, No 2 (2020); 305-314
Mimicry between respiratory virus proteins and some human immune proteins
https://iimmun.ru/iimm/article/download/1179/5904
https://iimmun.ru/iimm/article/download/1179/5905
https://iimmun.ru/iimm/article/download/1179/5906
https://iimmun.ru/iimm/article/download/1179/5907
https://iimmun.ru/iimm/article/download/1179/5908
https://iimmun.ru/iimm/article/download/1179/5909
https://iimmun.ru/iimm/article/download/1179/5910
https://iimmun.ru/iimm/article/download/1179/7160
https://iimmun.ru/iimm/article/download/1179/7193
https://iimmun.ru/iimm/article/download/1179/7194
https://iimmun.ru/iimm/article/download/1179/7201
https://iimmun.ru/iimm/article/download/1179/7364
https://iimmun.ru/iimm/article/download/1179/7716
https://iimmun.ru/iimm/article/download/1179/8480
Zhilinskaya I.N.; Smorodintsev Institute of Influenza, Ministry of Health of the Russian Federation
2020-04-16 14:31:55
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1179
мимикрия; вирусные белки; белки иммунной системы
ru
A comparative analysis on search for amino acid sequences in viral proteins causing respiratory infections (or respiratory infections syndrome) homologous to amino acid sequences from some human immune proteins was performed. The following viruses were used for comparative computer analysis: coronavirus (SARS-CoV), serotype C subgroup adenovirus C (adenoid 71 strain), measles virus (ICHINOSE-BA strain), rubella (Therien strain) and respiratory syncytial (B1 strain) virus. The search for homologous sequences in viral and human immune proteins was carried out by computer comparison of 12 amino acid fragments, which were assigned as homologous at identity in ≥ 8 positions. The data obtained showed that viral proteins contained homologous motifs in several host immune proteins involved in regulating both the inflammatory response and immune response. Mechanistically, all viruses studied were characterized by sequences homologous to host immune proteins such as complement system proteins, integrins, apoptosis inhibitory proteins, interleukins, and toll-like receptors. Such cellular proteins are actively involved in regulating host inflammatory process and immune response formation. Upon that, a set of host immune proteins, to which homologous fragments were found in viral proteins, was individual for each virus. Interestingly, the largest amount of homologous fragments (up to 20) was mainly concentrated in viral proteins with polymerase and protease activity suggesting that these proteins apart to their major role were involved in production of viral nucleic acids and might participate in regulating host immune system. Envelope, internal and non-structural viral proteins, homologous fragments were detected in much smaller quantities (from 1 to 4). In addition, two fragments homologous to various motifs of the same cellular protein were detected in some viral proteins. Thus, the data obtained further support our understanding that signs of immune system disorders in viral infections can result from multi-layered processes associated with modulation of host innate and adaptive immune system, and open up new approaches to study interaction of viruses with host immune system and identify new functions of viral proteins.
oai:iimmun.ru:article/9641
2023-12-15T21:48:01Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/9641
2023-12-15T21:48:01Z
Russian Journal of Infection and Immunity
Vol 13, No 5 (2023); 864-872
Alteration in innate immune cues assessed by analyzing peripheral blood immune system in post-covid patients
https://iimmun.ru/iimm/article/download/9641/87187
https://iimmun.ru/iimm/article/download/9641/87188
https://iimmun.ru/iimm/article/download/9641/87189
https://iimmun.ru/iimm/article/download/9641/87190
https://iimmun.ru/iimm/article/download/9641/87191
https://iimmun.ru/iimm/article/download/9641/87192
https://iimmun.ru/iimm/article/download/9641/87193
https://iimmun.ru/iimm/article/download/9641/87194
https://iimmun.ru/iimm/article/download/9641/87195
https://iimmun.ru/iimm/article/download/9641/87196
https://iimmun.ru/iimm/article/download/9641/87197
https://iimmun.ru/iimm/article/download/9641/153551
Dobrynina M.A.; Institute of Immunology and Physiology, Ural Branch of the Russian Academy of Sciences; State Research Center of the Russian Federation — Federal Medical Biophysical Center named after A.I. Burnazyan of the Federal Medical and Biological Agency of the Russian Federation
Zurochka A.V.; Institute of Immunology and Physiology, Ural Branch of the Russian Academy of Sciences; South Ural State University (National Research University)
Komelkova M.V.; Institute of Immunology and Physiology, Ural Branch of the Russian Academy of Sciences; South Ural State University (National Research University)
Zurochka V.A.; Institute of Immunology and Physiology, Ural Branch of the Russian Academy of Sciences; South Ural State University (National Research University)
Praskurnichiy E.A.; State Research Center of the Russian Federation — Federal Medical Biophysical Center named after A.I. Burnazyan of the Federal Medical and Biological Agency of the Russian Federation
Ryabova L.V.; South Ural State Medical University of the Ministry of Health of the Russian Federation
Sarapultsev A.P.; Institute of Immunology and Physiology, Ural Branch of the Russian Academy of Sciences; South Ural State University (National Research University)
2023-11-30 23:30:48
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/9641
SARS-CoV-2 инфекция; постковидные пациенты; иммунная система; натуральные киллеры (NK-клетки); Т-клетки; Т-лимфоциты; ТNK-лимфоциты; В-лимфоциты; CD46
Array
ru
The COVID-19 pandemic, caused by the SARS-CoV-2 virus, has led to global morbidity and mortality. Some patients fully recover from COVID-19, whereas around 45% subjects suffer from various persistent symptoms (fatigue, cognitive impairment, impaired thermoregulation, skin diseases, etc.) for at least four months after SARS-CoV-2 infection regardless of disease severity. Such persistent post-infection effects are known as long-COVID, post-acute effects of COVID-19, or post-COVID state. SARS-CoV-2 infection is accompanied by damage to the innate immune system. Considering the role of natural killer cells and the activation of the complement system in COVID-19 as well as the regulatory properties related to CD46 and its potential involvement in cell virus entry, we found necessary to study immune system parameters associated with impairment of these innate immune cues on various leukocyte subpopulations in post-COVID patients. We studied 92 immune system parameters, including: pan-leukocyte markers for gated lymphocytes, phenotyping of T cells, T-helper inducers, cytotoxic T-lymphocytes, NK- and TNK-cells, T-regulatory cells/suppressors, B-lymphocytes, including B-memory cells, activated helpers, early activation of lymphocytes, activated T-lymphocytes, and late lymphocyte activation markers. Levels of total IgM, IgG, IgA, specific IgM, IgG to coronavirus COVID-19, C1-inhibitor, C3a, and C5a complement components were measured by enzyme immunoassay using Multiscan FC Thermoscientific enzyme immunoassay analyzer (China) and Vector Best reagent kits (Russia). A complete blood count was conducted to study 25 parameters: leukocyte, erythrocyte, and platelet hematopoietic lineages as well as the quantitative and qualitative composition of hematopoietic lineages. Our study results showed that in some patients, six months after suffering from COVID-19, there was a decrease in the level of NK cells (48%) and CD46+ pan-leukocyte marker cells (64.5%). A decrease in NK cell levels was accompanied by increased level of total T- and B-lymphocytes, and altered platelet and erythroid hematopoietic lineages. In patients with reduced CD46 expression on T-lymphocytes, both their total count and NK cell count were significantly reduced. Our data also suggest that CD46 might be potentially involved in development of SARS-CoV-2 infection and the post-COVID state. Thus, in 50–65% of patients who have experienced SARS-CoV-2 infection, damage to the innate immune system persists after six months being accompanied by impaired erythroid and platelet hematopoietic lineages. The data obtained indicate a need for using immunocorrective therapy in such patients.
oai:iimmun.ru:article/557
2022-04-12T15:20:27Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/557
2022-04-12T15:20:27Z
Russian Journal of Infection and Immunity
Vol 7, No 3 (2017); 251-258
SEPSIS AT HIV-POSITIVE PATIENTS
Puzyryova L.V.; Omsk State Medical University
Konchenko V.D.; D.M. Dalmatov Infectious Hospital No. 1
Dalabayeva L.M.; D.M. Dalmatov Infectious Hospital No. 1
2017-09-28 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/557
сепсис; ВИЧ-инфекция; иммуносупрессия; грамположительный сепсис; грамотрицательный сепсис; тромбоцитопения; инфекционный эндокардит
ru
Septic implications at patients aren’t a rarity, especially in surgical, pediatric and obstetric practice now. However not enough attention, despite epidemic of this disease is paid to implication of a sepsis at patients with HIV infection now. Clinical laboratory implications of a sepsis at 36 patients with HIV infection are analysed. The main group is presented by patients with a lethal outcome (n = 18), control — with a positive outcome of a stationary stage of treatment (n = 18). Work is carried out on materials of the Infectious hospital No. 1 of D.M. Dalmatov of Omsk. At HIV-positive patients the stage of secondary diseases met most more often. Anti-retrovirus therapy was accepted only by 33% of patients in control group. At the died patients the hyperthermia arose after development of complaints from organs and systems and lasted less than 15 days. Low indicators of the CD4+ lymphocytes and a high virus load of HIV in a blood of patients with a lethal outcome became perceptible. In the general blood test thrombocytopenias, eosinopenias, monocytopenias, in the biochemical analysis — augmentation of the general bilirubin, thymol turbidity test, prothrombin ratio, urea were more often taped. At a bacteriological blood analysis various microflora with prevalence of S. aureus, sometimes in combination with Gram-negative flora was taped. Symptoms of an infectious endocarditis were taped at every second dead of the HIV-positive patient. Almost at all patients radiological changes in a pulmonary tissue were taped. Thus, clinical implications at patients with HIV infection are few symptoms and not typicalness that demands further studying.
oai:iimmun.ru:article/1419
2022-04-12T04:49:13Z
iimm:ORIG
oai:iimmun.ru:article/787
2023-08-06T17:26:35Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/787
2023-08-06T17:26:35Z
Russian Journal of Infection and Immunity
Vol 8, No 3 (2018); 355-360
MODIFYING EFFECT OF INHALATION CHEMICAL LOAD ON THE CONTENT OF CYTOKINES AND IMMUNOGLOBULINS IN ADOLESCENTS WITH LAMBLIA INFECTION
Masnavieva L.V.; East-Siberian Institute of Medical and Ecological Research.
Kudaeva I.V.; East-Siberian Institute of Medical and Ecological Research.
2018-11-02 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/787
подростки; иммунная система; цитокины; иммуноглобулины; лейкоциты; лямблии; загрязнение воздушной среды
ru
Giardiasis ranks second in the structure of parasitic diseases in Russia of the last decade. Infestation Giardia is associated with clinical signs of allergic reactions, imbalance parameters of the T- and В-lymphocytes, changes in the concentration of cytokines and immunoglobulins. Air pollution by chemical compounds also affects the immune system, stimulates production of pro- and anti-inflammatory cytokines, immunoglobulins. It can be assumed that the response of the organism to lamblia invasion will undergo changes under conditions of chemical inhalation exposure. The purpose of this study was to evaluate the content of cytokines and immunoglobulins in adolescents with giardiasis with an inhalation chemical load. 295 adolescents aged 13–16 with I–III health groups who did not have any at the time of the examination, exacerbation of any diseases and complaints of the work of gastrointestinal organs were included in the survey. Cellular blood composition with differential count of the leukocyte formula were studied in schoolchildren. The content of antibodies to lamblia, interleukins-2 and -10, interferons-alpha and-gamma, immunoglobulins A were studied in the blood serum by the method of enzyme immunoassay. Evaluation of individual inhalation chemical load by substances tropic to the immune system was carried out for each person. Data on the content of impurities in ambient air, indoor air, information on the organization of educational process and rest of students and their anthropometric parameters were taken into account when calculating chemical loads. Groups were formed after evaluation of antibodies to lamblia. Persons with Giardia invasion made up group I, school children without giardiasis were included in group II. Subgroups with a hazard index of immunity less than 2, 2 or more selected in each group. It has been established that changes in the relative content of basophils and monocytes, an increase in the levels of interferons-alpha and gamma, interleukin-2 and immunoglobulin A in adolescents with giardiasis occur at indexes of the development of pathology of the immune system less than 2. Adolescents with hazard indices of immunity equal to 2 or more decreased the content of immunoglobulin A and interferon-gamma and no differences in concentrations of interleukin-2 and -10, interferon-alpha and indicators of leukogram with parasitic infestation, compared to persons without giardiasis. It can be assumed, that high levels of inhalation chemical load, affecting the immune system of adolescents, create the prerequisites for the deficiency of protective mechanisms and the lamblias persistence.
oai:iimmun.ru:article/1927
2023-11-17T08:45:50Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/1927
2023-11-17T08:45:50Z
Russian Journal of Infection and Immunity
Vol 12, No 4 (2022); 726-734
The clinical effectiveness of probiotics and autoprobiotics in treatment of <i>Helicobacter pylori</i>-associated dyspepsia
https://iimmun.ru/iimm/article/download/1927/0
https://iimmun.ru/iimm/article/download/1927/13048
https://iimmun.ru/iimm/article/download/1927/13049
https://iimmun.ru/iimm/article/download/1927/13050
https://iimmun.ru/iimm/article/download/1927/13051
https://iimmun.ru/iimm/article/download/1927/13052
https://iimmun.ru/iimm/article/download/1927/13053
https://iimmun.ru/iimm/article/download/1927/13054
https://iimmun.ru/iimm/article/download/1927/13055
https://iimmun.ru/iimm/article/download/1927/13056
https://iimmun.ru/iimm/article/download/1927/13057
https://iimmun.ru/iimm/article/download/1927/13079
https://iimmun.ru/iimm/article/download/1927/13080
https://iimmun.ru/iimm/article/download/1927/13902
https://iimmun.ru/iimm/article/download/1927/14359
https://iimmun.ru/iimm/article/download/1927/14360
https://iimmun.ru/iimm/article/download/1927/14361
https://iimmun.ru/iimm/article/download/1927/14362
https://iimmun.ru/iimm/article/download/1927/14363
Ermolenko E.I.; Institute of Experimental Medicine; North-Western State Medical University named after I.I. Mechnikov
Molostova A.S.; Institute of Experimental Medicine; North-Western District Scientific and Clinical Center named after L.G. Sokolov, Federal Medical and Biological Agency
Baryshnikova N.V.; Institute of Experimental Medicine; Pavlov First Saint Petersburg State Medical University; St. Petersburg State Pediatric Medical University
Svarval A.V.; St. Petersburg Pasteur Institute
Gladyshev N.S.; St. Petersburg Pasteur Institute; St. Petersburg State University
Kashchenko V.A.; St. Petersburg State University; North-Western District Scientific and Clinical Center named after L.G. Sokolov, Federal Medical and Biological Agency
Suvorov A.N.; Institute of Experimental Medicine; St. Petersburg State University
2022-11-15 12:49:10
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1927
Helicobacter pylori; эрадикация; пробиотики; аутопробиотики; энтерококки; Enterococcus faecium
Array
en
The aim of our study was to evaluate the clinical performance of a monotherapy by Enterococcus faecium-based probiotics and indigenous autoprobiotics against H. pylori associated dyspepsia.
Materials and methods. There were examined 95 patients with dyspepsia. The entire patient cohort underwent clinical evaluation including filling out the questionnaire to assess dyspepsia symptoms before and after treatment, gastric endoscopy as well as gastric multi-focal biopsy (gastric body and gastric antrum) and verification of H. pylori infection with the three clinical laboratory methods (biochemical, bacteriological and molecular detection). An antagonistic in vitro activity of probiotics against H. pylori was detected by drop plate method for probiotic strains Enterococcus faecium SF68 and Bifidobacterium bifidum (Bifiform), Enterococcus faecium L3 (Laminolact), and autoprobiotic strains combined with indigenous Enterococcus faecium. To examine an antagonistic activity of probiotics and autoprobiotics in clinical trials, we used a starter culture based on the Enterococcus faecium L3 strain and an autoprobiotic based on indigenous Enterococcus faecium. The probiotic or autoprobiotic were administered orally to patients with gastritis twice a day at dose of 50 ml (8.0 lgCFU/ml) for 20 days. H. pylori eradication was assessed by stool antigen test 1.5–2 months after the end of treatment.
Results. Initially the H. pylori infection was confirmed with 49.4% of patients. The sensitivity of H. pylori to the probiotics was detected in 81% of individuals for indigenous Enterococci (the autoprobiotic), 76% — for Laminolact, and in 62% — for Bifiform. 22 patients with previous history of allergic reactions to antibiotics used in routine H. pylori eradication regimens were divided in two cohorts. One cohort (10 patients) received the autoprobiotic only, another cohort (12 patients) received only probiotic. Monotherapy with autoprobiotic resulted in 100% H. pylori eradication, single-agent therapy with probiotic led to 60% eradication of H. pylori. Dyspepsia symptoms were completely resolved in both groups of patients.
Conclusion. Our research demonstrated the sensitivity of examined H. pylori strains to be similar for traditional eradication treatment agents (antibiotics) and the proposed intervention agents (probiotics and autoprobiotics). An autoprobiotic monotherapy with indigenous enterococci led to higher levels of H. pylori eradication than with E. faecium L3-based probiotic agent. Our work demonstrated advantage for application of probiotics in patients with antibiotic allergies or other obstacles for the standard eradication therapy. Nonetheless, further investigation to better understand underlying mechanisms of action, as well as larger observational and randomized studies, are necessary to determine the scope of therapeutic application for probiotics and autoprobitics to eradicate H. pylori infection.
oai:iimmun.ru:article/121
2022-04-12T21:21:39Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/121
2022-04-12T21:21:39Z
Russian Journal of Infection and Immunity
Vol 3, No 1 (2013); 37-42
ANTIBIOTIC RESISTANCE OF THE BACTERIA ISOLATES OBTAINED FROM THE PATIENTS WITH DIABETES AND HIV-INFECTED PATIENTS IN CARDIOSURGICAL HOSPITAL
Yagodina A.Y.; ГБУЗ Пермского края Пермская краевая клиническая больница № 2 «Институт Сердца», г. Пермь
Pegushina O.G.; ГБУЗ Пермского края Пермская краевая клиническая больница № 2 «Институт Сердца», г. Пермь
Maslov Y.N.; ГБОУ ВПО Пермская государственная медицинская академия им. акад. Е.А. Вагнера МЗ РФ, г. Пермь
Serova I.A.; ГБОУ ВПО Пермская государственная медицинская академия им. акад. Е.А. Вагнера МЗ РФ, г. Пермь
Naumov S.A.; ГБУЗ Пермского края Пермская краевая клиническая больница № 2 «Институт Сердца», г. Пермь
2014-07-07 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/121
антибиотикорезистентность; кардиохирургия; ВИЧ-инфекция; сахарный диабет; иммунодефицит; внутрибольничная инфекция
ru
Abstract. The study was performed in the Perm Krai Clinical Hospital N 2 “Heart Institute”. The aim of this study was to evaluate the antibiotic susceptibility in isolates from patients with diabetes mellitus and HIV-infected patients. In all groups involved in the study (patients with diabetes mellitus, patients with HIV infection, and control group) the predomination of the Gram-positive species have been revealed. This fact is typical for surgical departments. More isolates of Staphylococcus aureus were found in the group of HIV-infected patients compared to patients in the control group (20.6% vs 4.6%, p = 0.02, respectively). Oxacillin resistance rate in Staphylococcus aureus was higher in the group of HIV-infected patients than in patients from the control group. Significant differences in Staphylococcus epidermidis resistance rate between HIV-infected patients and patients in the control group were detected for oxacillin (60% vs 10%, p = 0.03, respectively). It is plausible that in the group of immunocompromised patients vancomycin might be recommended for empiric therapy of infections caused by gram-positive species. The Enterococcus faecalis was more often isolated from HIV-infected patients in compare with patients from the control group (34.5% vs 13.8%, p = 0.02), probably due to severe immunodeficiency in the late stages of HIV infection. The absence of significant differences in resistance rate in Staphylococcus aureus and Staphilococcus epidermidis between patients with diabetes and patients in the control group could be considered as important. This fact might be associated with judicious control of glycemia during the pre-, intra-, and postoperative time periods. Postoperative pyoinflammatory diseases remain serious obstacle for the progress in cardiosurgery. Consequently, it’s important to know the characteristics of the microflora, colonizing the patients.
oai:iimmun.ru:article/2009
2023-04-01T16:28:37Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/2009
2023-04-01T16:28:37Z
Russian Journal of Infection and Immunity
Vol 13, No 1 (2023); 55-66
Maintaining immunological memory to the SARS-CoV-2 virus during COVID-19 pandemic
https://iimmun.ru/iimm/article/download/2009/14072
https://iimmun.ru/iimm/article/download/2009/14073
https://iimmun.ru/iimm/article/download/2009/14074
https://iimmun.ru/iimm/article/download/2009/14075
https://iimmun.ru/iimm/article/download/2009/14076
https://iimmun.ru/iimm/article/download/2009/14077
https://iimmun.ru/iimm/article/download/2009/14078
https://iimmun.ru/iimm/article/download/2009/14079
https://iimmun.ru/iimm/article/download/2009/14080
https://iimmun.ru/iimm/article/download/2009/14081
https://iimmun.ru/iimm/article/download/2009/14082
https://iimmun.ru/iimm/article/download/2009/14083
https://iimmun.ru/iimm/article/download/2009/14084
https://iimmun.ru/iimm/article/download/2009/15143
https://iimmun.ru/iimm/article/download/2009/74056
https://iimmun.ru/iimm/article/download/2009/74057
https://iimmun.ru/iimm/article/download/2009/74058
https://iimmun.ru/iimm/article/download/2009/74059
https://iimmun.ru/iimm/article/download/2009/74060
Toptygina A.P.; G.N.Gabrichevsky Research Institute for Epidemiology and Microbiology; Lomonosov Moscow State University
Afridonova Z.E.; G.N.Gabrichevsky Research Institute for Epidemiology and Microbiology
Zakirov R.S.; Federal State Autonomous Institution “National Medical Research Center of Children’s Health” of the Ministry of Health of the Russian Federation
Semikina E.L.; Federal State Autonomous Institution “National Medical Research Center of Children’s Health” of the Ministry of Health of the Russian Federation; I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation
2023-04-01 18:41:14
Authors who publish with this journal agree to the following terms:
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Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/2009
COVID-19; SARS-CoV-2; антитела; вакцинация; гибридный иммунитет; клеточный иммунитет; прорывной иммунитет
Array
НИОКТР 121021100125-4 от 10.02.2021г.
ru
The question on the duration and effectiveness of post-infection vs post-vaccination SARS-CoV-2 immunity remains in the focus of numerous studies. The aim of the work was to examine the duration of maintained post-infection and post-vaccination SARS-CoV-2 immunity as well as formation of hybrid (vaccination after infection) and breakthrough (repeated disease or disease after vaccination) immunity in the context of an ongoing COVID-19 pandemic. 107 adults with mild or moderate COVID-19 3–18 months after the disease and 30 subjects vaccinated twice with the Sputnik V vaccine were examined 1–6 times. Antibodies against SARS-CoV-2 virus were determined by ELISA on the “SARS-CoV-2-IgG quantitative-ELISA-BEST” test systems. The antibody avidity was measured by additional incubation with and without denaturing solution. Mononuclear cells were isolated from blood by gradient centrifugation, incubated with and without coronavirus S-protein for 20 hours, stained with fluorescently labeled antibodies, and the percentage of CD8highCD107a+ was counted using FACSCanto II cytometer. It was shown that in the group of convalescent and vaccinated subjects, the level of virus-specific antibodies decreased more deeply in individuals with initially high humoral response, but 9 months later the decrease slowed down and reached a plateau. The antibody avidity rose up to 50% and persisted for 18 months. Cellular immunity in recovered patients did not change for 1.5 years, while in vaccinated patients it gradually decreased 6 months later, but remained at detectable level. After revaccination, a significant increase in the level of antibodies, avidity up to 67.6% and cellular immunity returned to the initial level were noted. Hybrid immunity turned out to be significantly higher than post-infection and post-vaccination immunity. The level of antibodies increased to 1218.2 BAU/ml, avidity — to 69.85%, and cellular immunity — to 9.94%. Breakthrough immunity was significantly higher than that after the first disease. The level of antibodies rose to 1601 BAU/ml, avidity — up to 81.6%, cellular immunity — up to 13.71%. Using dynamic observation of four COVID-19 convalescents, it has been shown that in the context of the ongoing pandemic and active coronavirus mutation, natural boosting occurs both asymptomatically and as a result of a mild re-infection, which prevents disappearance of SARS-CoV-2 humoral and cellular immunity.
oai:iimmun.ru:article/247
2016-07-05T07:39:22Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/247
2016-07-05T07:39:22Z
Russian Journal of Infection and Immunity
Vol 4, No 4 (2014); 359-364
CLINICAL AND IMMUNOLOGICAL CHARACTERISTICS OF PATIENTS WITH HERPES INFECTIONS OF VARYING SEVERITY
Lyuboshenko T.M.; Omsk State Medical Academy, Russian Ministry of Health, Omsk, Russian Federation 644009, Russian Federation, Omsk, Maslennikova str., 150. Phone: +7 (3812) 36-36-83 (office)
Dolgikh T.I.; Clinical Diagnostic Centre, Omsk, Russian Federation
2015-02-03 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/247
герпесвирусная инфекция; клинические проявления; диагностика; иммунный профиль; тяжесть течения
ru
The peculiarities of clinical signs, immune and interferon status in 180 patients with laboratory confirmed infection of varying severity, caused by herpes simplex virus (VSHI) have been studied. It was determined that frequency of bacterial infections is increased in patients with more severe clinical forms of VSHI. In patients with mild course furunculosis was more often detected than in other groups. In patients with moderate course of VSHI vaginal candidiasis was more common. In patients with severe VSHI course the combination of labial and genital herpes as well as infection caused by the human papilloma virus were more prevalent. In case of severe infection occurred an increased frequency of dysbiosis, fatigue, low grade temperature, iron deficiency anemia and malignancies. The highest frequency of allergic reactions is observed in patients with moderate course of VSHI. The autoimmune syndrome manifestations were not depend on the severity of VSHI. The degree of reduction of cell immunity and disorders in the system of interferon were closely related to severity of VSHI course.
oai:iimmun.ru:article/399
2022-04-12T15:18:25Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/399
2022-04-12T15:18:25Z
Russian Journal of Infection and Immunity
Vol 6, No 2 (2016); 141-150
MOLECULAR EPIDEMIOLOGY FEATURES OF HBV/HDV CO-INFECTION IN KYRGYZSTAN
Semenov A.V.; St. Petersburg Pasteur Institute;
Pavlov First St. Petersburg State Medical University;
North-West State Medical University named after I.I. Mechnikov
Ostankova Y.V.; St. Petersburg Pasteur Institute, St. Petersburg
Nogoybaeva K.A.; Kyrgyz State Medical Institute of Retraining and Skills
Kasymbekova K.T.; Kyrgyz State Medical Institute of Retraining and Skills
Lavrentieva I.N.; St. Petersburg Pasteur Institute
Tobokalova S.T.; Kyrgyz State Medical Institute of Retraining and Skills
Totolian A.A.; St. Petersburg Pasteur Institute;
Pavlov First St. Petersburg State Medical University
2016-06-24 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/399
гепатит D;гепатит В;сочетанная инфекция;молекулярная эпидемиология;секвенирование;Кыргызстан
ru
One of the most serious health problems in the world are hepatotropic viruses that cause chronic liver disease. Hepatitis B virus is distributed globally; around 5% of the carriers are also infected with hepatitis delta virus. Co-infection or superinfection of hepatitis viruses B and D significantly associated with a much more severe liver disease, compared with infection only hepatitis B virus. However, examination of hepatitis virus B carriers for the presence of hepatitis D virus in most regions of the world is not mandatory. It should be noted that the complete genotype mapping of viruses hepatitis B and D isolated on the territory of the CIS and the countries of the former Soviet Union, there is not yet, despite the constantly ongoing works devoted genotyping hepatotropic virus in the territory of the Russian Federation and neighboring countries. Due to the fact that one of the prospective ways of spreading viruses is the “labor migration” the inhabitants of Central Asia in other countries, including the Russian Federation, there is a need to pay attention to the situation of viral hepatitis in the region. The aim of our study was to estimate the prevalence of genetic variants and characteristics of molecular epidemiology of chronic viral hepatitis co-infection B + D in Kyrgyzstan. The study involved 30 plasma samples from patients with chronic viral hepatitis B and D from different regions of Kyrgyzstan. Based on the phylogenetic analysis of the isolates showed that among patients examined HBV identified only D genotype. Based on the phylogenetic analysis of the isolates indicated that among the examined patients with chronic viral hepatitis B revealed only genotype D. It is shown prevalence of HBV subtype D1 (73.34%) compared to the HBV subtype D2 (3.33%) and D3 (23.33%). Revealed HDV genotype I with highly variable region of the gene encoding the delta antigen. The high similarity of some isolates with strains specific to neighboring countries endemic for hepatotropic viruses, as well as a dense clustering of other isolates may be an indication of numerous independent drifts of strains into the territory of the country. Also it can talk about the speed of evolution of the virus in a geographically isolated region as Kyrgyzstan. Identification of the propagation characteristics and endemics role in circulation of genotype of hepatitis B and D is great importance.
oai:iimmun.ru:article/1660
2023-08-06T17:09:06Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/1660
2023-08-06T17:09:06Z
Russian Journal of Infection and Immunity
Vol 11, No 6 (2021); 1083-1088
<i>Toxoplasma gondii</i> infection in patients with malignant and benign bone tumours
https://iimmun.ru/iimm/article/download/1660/10186
https://iimmun.ru/iimm/article/download/1660/10187
https://iimmun.ru/iimm/article/download/1660/10188
https://iimmun.ru/iimm/article/download/1660/10189
https://iimmun.ru/iimm/article/download/1660/11395
Hajizadeh M.; Iran University of Medical Sciences
Falak R.; Iran University of Medical Sciences
Tavakoli-Yaraki M.; Iran University of Medical Sciences
Hosseinzadeh R.; Iran University of Medical Sciences
Alipour M.; Iran University of Medical Sciences
Ahmadpour E.; Tabriz University of Medical Sciences
Rafiei-Sefiddashti R.; Iran University of Medical Sciences
2021-07-14 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1660
первичная опухоль кости; злокачественная опухоль; доброкачественная опухоль; токсоплазмоз; Toxoplasma gondii; ПЦР в реальном времени
en
Toxoplasma gondii (T. gondii) is an intracellular parasite that infects humans, and seroprevalence of its infection varies from about 10 to 80 percent in different countries with a higher prevalence in warmer and humid regions. In this study, the rate of acute and chronic toxoplasmosis in patients with benign or malignant bone tumours was investigated. Fifty-three patients who suffered from various bone tumours, as well as sixty-five healthy controls with an unknown sero-logical profile for anti-Toxoplasma antibodies, were enrolled in this cross-sectional study. Anti-Toxoplasma antibodies were detected in serum samples using enzyme-linked immunosorbent assay (ELISA) and blood samples of them were used for real-time PCR. Thirty-two (60.32%) and twenty-one (39.63%) of patients had malignant tumours and benign tumours, respectively. The results showed a higher and significant seropositivity rate of IgM antibodies in primary bone tumour patients compared to the control group and Toxoplasma DNA became positive in 18.86% of patients with primary bone tumours and 6.15% of controls. Surprisingly, the high presence of parasite DNA was detected in patients with malignant tumours. The seroprevalence of T. gondii IgM antibodies and DNA positivity among the cancer patients were significantly higher than healthy individuals. Also, chronic toxoplasmosis (it was shown with IgG positive) appears to be more common in people with benign cancers than malignancies. The study showed a relatively high seroprevalence of anti-T. gondii antibodies in patients with primary bone cancer. However, the considerable rate of positive blood samples for the presence of parasite’s DNA should not be ignored. A key to the effective management of diseases in immunosuppressed individuals is prompt and accurate diagnosis of toxoplasmosis. Moreover, it seems that PCR tests may be more reliable than serological methods and it could be considered as a precise method for diagnosis of acute toxoplasmosis.
oai:iimmun.ru:article/1744
2023-11-19T09:12:09Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/1744
2023-11-19T09:12:09Z
Russian Journal of Infection and Immunity
Vol 12, No 2 (2022); 315-322
Genetic polymorphisms of <i>helicobacter pylori</i> clinical isolates in St. Petersburg, Russia
https://iimmun.ru/iimm/article/download/1744/11073
https://iimmun.ru/iimm/article/download/1744/11074
https://iimmun.ru/iimm/article/download/1744/11075
https://iimmun.ru/iimm/article/download/1744/11076
https://iimmun.ru/iimm/article/download/1744/11077
https://iimmun.ru/iimm/article/download/1744/11078
https://iimmun.ru/iimm/article/download/1744/11079
https://iimmun.ru/iimm/article/download/1744/11080
https://iimmun.ru/iimm/article/download/1744/11081
https://iimmun.ru/iimm/article/download/1744/11898
https://iimmun.ru/iimm/article/download/1744/11899
https://iimmun.ru/iimm/article/download/1744/11900
https://iimmun.ru/iimm/article/download/1744/12689
https://iimmun.ru/iimm/article/download/1744/12919
https://iimmun.ru/iimm/article/download/1744/12920
Svarval A.V.; St. Petersburg Pasteur Institute
Starkova D.A.; St. Petersburg Pasteur Institute
Ferman R.S.; St. Petersburg Pasteur Institute
Narvskaya O.V.; St. Petersburg Pasteur Institute; St. Petersburg State Research Institute of Phthisiopulmonology of the Ministry of Health of Russia
2022-05-13 14:39:21
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1744
Helicobacter pylori; ген cagA; ген vacA; ген oipA; гастрит; язва двенадцатиперстной кишки; рак желудка; гены вирулентности; геномный полиморфизм
Array
en
Introduction. Helicobacter pylori was proved to be the principal causative agent of gastroduodenal disorders in human. Although Russian Federation is among the countries with a high prevalence of H. pylori infection (60–90%), currently there is a very limited number of studies evaluating H. pylori genotypes in Russia. Objective. Based on the assessment of virulence-associated cagA, oipA, and vacA genes, our study was aimed to determine H. pylori genotypes associated with the clinical outcomes in patients with H. pylori infection in St. Petersburg, Northwest Russia. Materials and methods. Using PCR for the detection of cagA, oipA, and vacA s, m, i allelic variants, we analyzed 61 H. pylori isolates isolated and cultured from biopsies collected during endoscopy of patients with chronic gastritis (G), duodenal ulcer (DU), and gastric cancer (GC). Results. The genetic diversity of H. pylori clinical isolates has been revealed (HGDI 0.88): 41 (67%) of 61 H. pylori isolates were cagA-positive, 38 (62%) — oipA-positive. The proportions of cagA+ isolates differed in patients with G (56.7%) and DU (80.9%) (p = 0.06). The s, m, and i allelic variants of the vacA gene were detected in all strains, although the vacA s1 allele was significantly dominant in patients with DU (95.2%) rather than with G (64.9%) (p = 0.01). The vacA alleles m1 and i1 in the isolates from patients with G and DU were found in almost equal proportions: 45.9% and 42.8% for m1 allele, 45.9% and 47.6% for i1 allele, respectively. Seven isolates (11.5%) were positive for different mixed combinations of vacA alleles s, m, and i. Noteworthy, all vacA s2 strains were cagA-negative and had the m2 allele. OipA+ strains were found in almost equal proportions in patients with G (62.2%) and DU (57.1%) (p = 0.71). All three cagA- and oipA-positive isolates from patients with GC carried vacA s1/m1/i1 alleles. Different combinations of virulence-associated determinants constituted 17 genetic profiles. The most common combined genotype cagA+/oipA+/vacA s1/m1/i1 comprised 18 (29.5%) H. pylori isolates. Conclusion. We have determined predominant genotypes in the H. pylori population in the Northwest of Russia. The significant association between vacA s1 genotype of the pathogen and clinical manifestations of H. pylori infection has been established in our study.
oai:iimmun.ru:article/770
2023-08-06T17:10:19Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/770
2023-08-06T17:10:19Z
Russian Journal of Infection and Immunity
Vol 10, No 4 (2020); 735-740
VNTR loci as indicators of proline-dependent plague microbe strains (Yersinia pestis) in the central caucasian mountain natural plague focus
Dubyanskiy V.M.; Stavropol Research Anti-Plague Institute
Volynkina A.S.; Stavropol Research Anti-Plague Institute
Anisimov A.P.; Federal Budget Institution of Science «State Research Center for Applied Microbiology & Biotechnology»
2020-11-26 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/770
чума; природный очаг; штамм; VNTR локусы; MLVA; прогнозирование
Работа выполнена в рамках отраслевой научно-исследовательской программы Роспотребнадзора на 2016-2020 гг. «Проблемно-ориентированные научные исследования в области эпидемиологического надзора за инфекционными и паразитарными болезнями» (ДВМ и ВАС). АПА поддержан грантом Минобрнауки России (Соглашение № 075-15-2019-1671 от 31 октября 2019 г.)
ru
Y. pestis MLVA typing is used both to seek for similarities and differences between individual isolates upon conducting epidemiological investigations as well as for clonal clustering of intraspecies phylogenetic groups while analyzing microevolution and taxonomy issues. It cannot be ruled out that the most variable loci may be indicators allowing to approximate the unique strain-related properties circulating in certain natural plague foci. The Central Caucasian Highland Natural Plague Focus distinguished by heterogeneity of the circulating strains therein, including proline pro- and auxo-trophy, may represent a convenient model for testing this hypothesis. The purpose of our work was to assess the frequencies of the VNTR alleles associated with proline dependence among the Y. pestis strain VNTR loci, determined during previous MLVA-25 typing in the Central Caucasian Highland Natural Plague Focus. The main task was to identify the most informative sets of VNTR loci suitable for predicting proline pro— and auxotrophy (pro+, pro—). It was found that the loci ms45, ms56, ms46, ms07, ms69, ms62 displayed peak variability by allele frequencies and/or exhibited significant differences of mean allele frequencies in the pro— and pro+ strains. In particular, it was showed that the alleles of the ms45 locus contained 6 tandem repeats suggesting probability for pro+ reaching 0.944, whereas the alleles of the ms45 locus contained 7 tandem repeats with expected probability for pro— reaching 0.783. Moreover, the ms56 and ms46 contained 9 and more than 18 tandem repeats, respectively, thereby pointing at probability for pro+ equal to 0.933 and 0.818, respectively. Diagnostics for pro+/pro— phenotype by using specific statistical methods demonstrated statistical error 13.33% and 26.67% for the pro— and pro+ strains, respectively. All pro+ strains bearing a 6 tandem repeat complex from the ms45 locus, 9 tandem repeats derived from the ms56 locus and ms46 locus-derived 29—30 tandem repeats were accurately diagnosed solely based on these 3 loci. Thus, it is possible to predict some properties of Y. pestis strains based on determining the allele frequencies. While the number of MLVA typed plaque strains isolated in such natural focus has been progressively increased, it may be expected that opportunities for prognosing their properties based on determining locus tandem repeat composition having diagnostic value would be elevated.
oai:iimmun.ru:article/1452
2022-04-12T15:57:24Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/1452
2022-04-12T15:57:24Z
Russian Journal of Infection and Immunity
Vol 11, No 3 (2021); 523-529
Measles humoral immunity in health-care workers
https://iimmun.ru/iimm/article/download/1452/8370
https://iimmun.ru/iimm/article/download/1452/8371
https://iimmun.ru/iimm/article/download/1452/8372
https://iimmun.ru/iimm/article/download/1452/8373
https://iimmun.ru/iimm/article/download/1452/8374
https://iimmun.ru/iimm/article/download/1452/8375
https://iimmun.ru/iimm/article/download/1452/9948
Krieger E.A.; Northern State Medical University
Samodova O.V.; Northern State Medical University
2020-08-25 15:20:59
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1452
иммунитет; корь; вакцинация; антитела; серопревалентность; медицинские работники
ru
Healthcare-workers are at risk of contact with measles patients and disease transmission. Measles-infected employees of healthcare facilities may contribute to the nosocomial measles spread and serve as a source of infection for most susceptible cohorts such as pregnant women, neonates, and immunocompromised patients. In order to study the humoral immunity against measles in healthcare workers and reveal factors associated with seronegative status, we performed a cross-sectional study by enrolling 847 healthcare workers of the Arkhangelsk Regional Clinical Hospital. Anti-measles virus serum immunoglobulin G antibodies were quantified by using VectorMeasles-IgG ELISA kit (Vector-Best, Russia). According to the manufacturer's recommendations specific IgG anti-measles titer cut-off value higher than 0.18 IU/ml, equal to 0.12—0.17 IU/ml, or lower than 0.12 IU/ml was considered as positive (protective), equivocal, or negative, respectively. Assessing an impact of employee's gender, age, affiliation (department), current position was carried out by using binary logistic regression analysis while analyzing seronegative status of healthcare workers. Study participants dominated by females (92.1%). The median age was 48 (39; 57) years. The employees of somatic departments prevailed (26.7%). It was found that 93.7% of medical workers had concentration of anti-measles antibodies exceeding magnitude of protective titer (above 0.18 IU/ml), 4.4% and 1.9% were measles seronegative and equivocal, respectively. The level of antibodies against measles was associated with age of healthcare workers, but not with gender. All employees older than 60 years were measles seropositive, whereas those younger than 35 or within range 35 to 60 years of age had protective antibody titer in 77% and 95.5% of cases, respectively. The proportion of subjects with seronegative results did not depend on employee's position (doctor, nurse, cleaning worker), but varied a lot between different hospital departments. According to the data of logistic regression, the odds to have a seronegative test result among employees from somatic and remaining departments were 4.4-fold higher. Importantly, the rate of seronegative results was by 10-fold lower in employees born between 1968 and 1984 than those found in subjects born after 1985. On the other hand, subjects older than 60 years of age were seronegative at 50-fold lower rate than those who were under 35. The median concentration of measles immunoglobulin G among vaccinated healthcare workers vs. subjects recovered after natural infection was significantly lower reaching 0.56 IU/ml and 4.2 IU/ml (p < 0.001), respectively. Five-year monitoring showed that titer of measles IgG antibodies decreased by 1.2-1.9-fold (average — 1.5). Thus, a cohort of healthcare workers from multidisciplinary healthcare facility demonstrated that the proportion measles-susceptible subjects was 6.3%. Importantly, age of examined subjects mainly affected seronegative status. Taking into consideration age-related lowered serum antibody level in vaccinated healthcare workers, seroprevalence studies and subsequent revaccination of seronegative people should be performed at least once every 5 years to prevent measles spread in healthcare facilities.
oai:iimmun.ru:article/713
2022-04-12T15:46:21Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/713
2022-04-12T15:46:21Z
Russian Journal of Infection and Immunity
Vol 9, No 2 (2019); 354-362
The problem of pertussis in some regions of the world
Basov A.A.; G.N. Gabrichevsky Research Institute for Epidemiology and Microbiology
Tsvirkun O.V.; G.N. Gabrichevsky Research Institute for Epidemiology and Microbiology
Gerasimova A.G.; G.N. Gabrichevsky Research Institute for Epidemiology and Microbiology
Zekoreeva A.K.; Center for Hygiene and Epidemiology of the Moscow in the Northern District
2019-05-13 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/713
коклюш; бесклеточные вакцины; иммунизация; ревакцинация; календарь прививок; заболеваемость
ru
Pertussis infection remains a high-priority issue both for Russian health care and abroad. A rise of pertussis incidence in various human age groups instigates a search for new ways to fight this infection and improve methods for its laboratory diagnostics. By taking into consideration a short-term effect induced by acellular and whole-cell vaccines, a feasibility of introducing the second or even the third pertussis revaccination is vigorously debated. Objective of the study was to analyze the experience and effectiveness of acellular pertussis vaccines in countries, which use the second and third pertussis revaccination in the National Immunization Schedule in order to have an insight into adjusting strategy and tactics of pertussis immunization In Russia. Analyzing pertussis prevalence demonstrated that despite a wide immunization coverage pertussis incidence in the last years (2008–2015) was increased in a large number of countries in the European region, as well as inAustralia,CanadaandUSA. However, the reasons for elevated pertussis incidence have not been clarified yet. On one hand, it may be accounted for by low vaccination coverage in adolescents and adults; weakened immune protection after vaccination; genetic changes in Bordetella pertussis; shortened durability of protective immunity in children vaccinated with acellular vs. whole-cell vaccine; improved monitoring and morbidity reporting, as well as improved laboratory diagnostics due to shifting from serological and bacteriological to molecular genetic assays. In an attempt to solve this issue, researchers from several countries collaborate to discuss and develop a strategy to reduce pertussis incidence. ForRussia, the most important is to empower and/or improve existing infant immunization strategy in order to provide wide coverage with the four dose pertussis vaccine for decreasing the risk of pertussis morbidity and mortality. It is worth noting the “cocoon” strategy given the high risk of pertussis infection in children of the first months of life. We believe that forRussiait is worth investigating an opportunity of using children 2–3 months of life an acellular vaccine as the first vaccination, which is expected to increase the coverage of this cohort and allow to increase proportion of children who might complete vaccination by 5 months of age. At the same time, more reasonable might be to preserve a number of age groups for pertussis vaccination in the current National Immunization Schedule, as expanding age limits for vaccination might put a risk at increasing pertussis morbidity in older individuals, which could be hard to diagnose.
oai:iimmun.ru:article/690
2023-08-06T17:11:02Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/690
2023-08-06T17:11:02Z
Russian Journal of Infection and Immunity
Vol 10, No 1 (2020); 129-136
Level of measles herd immunity assessed in hospital medical workers within a framework of the state measles elimination program
https://iimmun.ru/iimm/article/download/690/3654
https://iimmun.ru/iimm/article/download/690/3655
https://iimmun.ru/iimm/article/download/690/3656
https://iimmun.ru/iimm/article/download/690/3657
https://iimmun.ru/iimm/article/download/690/3658
https://iimmun.ru/iimm/article/download/690/3659
https://iimmun.ru/iimm/article/download/690/6090
https://iimmun.ru/iimm/article/download/690/6137
https://iimmun.ru/iimm/article/download/690/6138
https://iimmun.ru/iimm/article/download/690/6139
https://iimmun.ru/iimm/article/download/690/6140
https://iimmun.ru/iimm/article/download/690/7765
Kostinov M.P.; Mechnikov Research Institute of Vaccines and Sera; I.M. Sechenov First Moscow State Medical University
Filatov N.N.; Mechnikov Research Institute of Vaccines and Sera; I.M. Sechenov First Moscow State Medical University
Zhuravlev P.I.; Mechnikov Research Institute of Vaccines and Sera
Gladkova L.S.; The City Clinical Hospital named after D.D. Pletnev of the Moscow City Health Department; Moscow State University of Food Industries
Polischuk V.B.; Mechnikov Research Institute of Vaccines and Sera
Shmitko A.D.; Mechnikov Research Institute of Vaccines and Sera
Pakhomov D.V.; Mechnikov Research Institute of Vaccines and Sera
Khromova E.A.; Mechnikov Research Institute of Vaccines and Sera
Vasilyeva G.V.; Mechnikov Research Institute of Vaccines and Sera
Tikhonova I.A.; The City Clinical Hospital named after D.D. Pletnev of the Moscow City Health Department
Ryzhov A.A.; Mechnikov Research Institute of Vaccines and Sera
Blagovidov D.A.; Mechnikov Research Institute of Vaccines and Sera
Kostinova A.M.; National Research Center Institute of Immunology
2020-04-05 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/690
коллективный иммунитет; корь; антитела к вирусу кори
ru
Within a framework of the state measles elimination program, in April, 2018 a level of measles herd immunity was assessed in 1899 Moscow hospital medical workers aged from 19 to 69 years and older. All subjects enrolled in the study were vaccinated against measles or recovered after measles infection. Serum samples were collected from subjects and examined by ELISA for measles IgG antibodies with the Vector-Best IgG-measle test system (Russia). It was found that 278 (14.6%) and 1621 (85.4%) subjects were seronegative (< 0.18 IU/mL) and seropositive (> 0.18 IU/ml), respectively. Age-related group distribution of 1855 serum samples revealed that percentage of seronegative subjects was in: aged 19—23 years was -38.5%; 24-28 and 29-33 years - 22.2%; 34-38 years - 27.5%; 39-43 years - 25.8%; 44-48 years - 16.8%; 49-53 and 54-58 years — 8.6% and 8.3%, respectively; 59-63 years old — 4.9%; 64-68 and over 69 years old — 0%. Moreover, mean level of measles IgG antibodies increased proportionally to age of medical workers from 0.58 IU/ml (19-23 years) to 2.94-2.72 IU/ml (64-68 and over 69 years). The data obtained indicate that a cohort of measles susceptible subjects (from 38.5% to 16.8%), respectively, is identified among young and middle age (from 19 to 48 years) individuals. It is assumed that two-dose measles vaccination in childhood does not contribute to the long-term preservation of protective levels of measles antibodies, thereby justifying a need to administer a three-dose measles vaccine.
oai:iimmun.ru:article/2036
2023-10-24T12:01:57Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/2036
2023-10-24T12:01:57Z
Russian Journal of Infection and Immunity
Vol 13, No 3 (2023); 535-548
Tick-borne infections in the south of Russia: modern epidemiological situation, new approach to create “forecasting” and “explaining” morbidity models (in astrakhan rickettsiosis fever and crimean-congo hemorrhagic fever)
https://iimmun.ru/iimm/article/download/2036/14469
https://iimmun.ru/iimm/article/download/2036/14471
https://iimmun.ru/iimm/article/download/2036/14472
https://iimmun.ru/iimm/article/download/2036/14473
https://iimmun.ru/iimm/article/download/2036/14474
https://iimmun.ru/iimm/article/download/2036/14475
https://iimmun.ru/iimm/article/download/2036/14476
https://iimmun.ru/iimm/article/download/2036/14477
https://iimmun.ru/iimm/article/download/2036/14478
https://iimmun.ru/iimm/article/download/2036/14479
https://iimmun.ru/iimm/article/download/2036/14480
https://iimmun.ru/iimm/article/download/2036/14481
https://iimmun.ru/iimm/article/download/2036/14728
https://iimmun.ru/iimm/article/download/2036/14729
https://iimmun.ru/iimm/article/download/2036/14730
https://iimmun.ru/iimm/article/download/2036/14925
https://iimmun.ru/iimm/article/download/2036/14926
https://iimmun.ru/iimm/article/download/2036/14927
https://iimmun.ru/iimm/article/download/2036/14928
https://iimmun.ru/iimm/article/download/2036/123638
https://iimmun.ru/iimm/article/download/2036/139468
Prislegina D.A.; Stavropol Plague Control Research Institute; Central Research Institute of Epidemiology
Maletskaya O.V.; Stavropol Plague Control Research Institute
Dubyanskiy V.M.; Stavropol Plague Control Research Institute; Central Research Institute of Epidemiology
Taran T.V.; Stavropol Plague Control Research Institute
Platonov A.E.; Central Research Institute of Epidemiology
2023-06-26 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/2036
клещевые трансмиссивные инфекции; климатические факторы; эпидемиологическая ситуация; прогнозирование заболеваемости; модель динамики заболеваемости «прогнозная»; модель динамики заболеваемости «объясняющая»
Array
19-75-20088
ru
The article presents a description of the current tick-borne infection epidemiological situation in the south of Russia from the years 2013 to 2022, proposes a new approach to develop “forecasting” models for morbidity dynamics of Astrakhan rickettsial fever (ARF) and Crimean hemorrhagic fever (СCHF) in the Astrakhan region and presents data assessing 2022 “explaining” models for the Stavropol Territory and Astrakhan Region. Materials and methods. A comprehensive research was performed using epidemiological analysis and non-parametric statistical methods. The data assessing tick-borne infections epidemic process manifestations were retrieved from ARF and CCHF morbidity databases (developed as a project) and documents of infectious disease focus epidemiological examination provided by the departments of Rospotrebnadzor in the subjects of the Southern and North Caucasian Federal Districts. Morbidity models were developed using the Bayes’ theorem and Wald’s sequential statistical analysis, with a preliminary calculation of indicators informativeness by the Kullback method. The values of climatic factors from the database of the Center for Collective Use “IKI-monitoring” of the Space Research Institute of the Russian Academy of Sciences were used. Results. The results of the study indicate persistence of serious epidemiological situation regarding rickettsiosis of the tick-borne spotted fever group, Q fever, tick-borne borreliosis and CCHF in the south of Russia. Almost all tick-borne infections nosological forms in children under 14 years (including young children and infants) were widely involved in the epidemic process, which belong to patients at risk for a complicated disease course due to complicated diagnostics and treatment. The annual registration of tick-borne infections cases in the resort areas, with the subsequent occurrence of imported cases in other, including non-endemic regions poses a serious problem. The proposed “forecasting” models allow to predict the CСHF and ARF morbidity for each administrative district of the Astrakhan region with up to 91.7% accuracy. The “explaining” models CСHF accuracy for the Stavropol Territory and Astrakhan Region, when tested in 2022, was 88.5 and 83.3%, respectively, for ARF — 91.7%. Conclusions. The further continuation of “forecasting” and “explaining” models verification for planning preventive measures and propose similar steps for tick-borne borreliosis and Q fever to epidemiological tick-borne infections to stabilize situation in the south of Russia.
oai:ojs.iimmun.ru:article/237
2016-02-16T10:54:36Z
iimm:ORIG
oai:iimmun.ru:article/16867
2024-02-17T14:59:08Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/16867
2024-02-17T14:59:08Z
Russian Journal of Infection and Immunity
Vol 13, No 6 (2023); 1079-1088
Influence of long-term antibiotic therapy on gut microbiome composition and metabolic profile in pulmonary tuberculosis
https://iimmun.ru/iimm/article/download/16867/151918
https://iimmun.ru/iimm/article/download/16867/151919
https://iimmun.ru/iimm/article/download/16867/151920
https://iimmun.ru/iimm/article/download/16867/151921
https://iimmun.ru/iimm/article/download/16867/151922
https://iimmun.ru/iimm/article/download/16867/151923
https://iimmun.ru/iimm/article/download/16867/151924
https://iimmun.ru/iimm/article/download/16867/151925
https://iimmun.ru/iimm/article/download/16867/151926
https://iimmun.ru/iimm/article/download/16867/151927
https://iimmun.ru/iimm/article/download/16867/151928
https://iimmun.ru/iimm/article/download/16867/154149
https://iimmun.ru/iimm/article/download/16867/154150
https://iimmun.ru/iimm/article/download/16867/154151
https://iimmun.ru/iimm/article/download/16867/154152
https://iimmun.ru/iimm/article/download/16867/154222
https://iimmun.ru/iimm/article/download/16867/158603
https://iimmun.ru/iimm/article/download/16867/158604
https://iimmun.ru/iimm/article/download/16867/158605
https://iimmun.ru/iimm/article/download/16867/158606
Yunusbaeva M.M.; ITMO University
Terentyeva D.R.; ITMO University; Pasteur Institute
Borodina L.Y.; Republican Clinical Antituberculous Dispensary
Zakirova A.M.; Republican Clinical Antituberculous Dispensary
Bulatov S.E.; Republican Clinical Antituberculous Dispensary
Bilalov F.S.; Republican Clinical Antituberculous Dispensary; Bashkir State Medical University
Yunusbayev B.B.; St. Petersburg State University
2023-12-25 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/16867
туберкулез; микробиом кишечника; дисбиоз; кишечные бактерии; метаболизм; антибиотики
Array
ru
The use of long-term multicomponent antibiotic therapy is the most effective way to treat tuberculosis (TB). However, little is known about the effect of this chemotherapy on the human intestinal microflora. The purpose of this study was to analyze an effect of long-term antibiotic therapy on gut microbiome composition and metabolic profile in TB patients. We used deep sequencing of fecal samples from 23 treatment-naive TB patients to reconstruct the metabolic capacity and strain/species-level abundance in the gut microbiome. Two fecal samples were obtained from each patient: before and after treatment. We showed that TB treatment regimen does not disrupt the overall diversity of the gut microbiome but does have an impact on gut bacterial microbiome composition and metabolic profile. While taking first-line anti-tuberculosis drugs (isoniazid, rifampicin, ethambutol, pyrazinamide), TB patients showed an apparent increase in Actinobacteria abundance. Pairwise comparison of metagenomic data revealed 28 differentially represented bacterial taxa, of which three species Bacteroides cellulosilyticus, Enterocloster aldensis, Clostridium spiroforme were strongly enriched in TB patients post-chemotherapy, whereas 25 species were enriched in TB patients before treatment (Bifidobacterium catenulatum, Enterococcus faecium, Bacteroides salyersiae, Bacteroides xylanisolvens, Bacteroides eggerthii, Lachnospira eligens, Akkermansia muciniphila, Ruminococcus lactaris, etc.) (p < 0.05). The metabolic profile of the gut microbiome was characterized by increased metabolic processes aimed at the growth and division of microbial cells. Iron is the main limiting factor for growth and reproduction. In addition, it is important to note the prevalence of glycolysis and lactate fermentation as the major means for energy production by intestinal microbiota.
oai:iimmun.ru:article/674
2022-04-12T15:21:36Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/674
2022-04-12T15:21:36Z
Russian Journal of Infection and Immunity
Vol 8, No 1 (2018); 61-70
INFECTION OF LABOUR MIGRANTS FROM CENTRAL ASIA AND RESIDENTS OF ST. PETERSBURG AND THEIR SUSCEPTIBILITY TO VARIOUS INFECTIOUS DISEASES
Kraeva L.A.; St. Peterburg Pasteur Institute; Military Medical Academy named S.M. Kirov
Тоkarevich N.K.; St. Peterburg Pasteur Institute
Lavrentyeva I.N.; St. Peterburg Pasteur Institute
Roshchina N.G.; St. Peterburg Pasteur Institute
Kaftyreva L.A.; St. Peterburg Pasteur Institute; North-Western State Medical University named after I.I. Mechnikov
Kunilova E.S.; St. Peterburg Pasteur Institute
Kurova N.N.; St. Peterburg Pasteur Institute
Stoyanova N.A.; St. Peterburg Pasteur Institute
Antipova A.Y.; St. Peterburg Pasteur Institute
Svarval А.V.; St. Peterburg Pasteur Institute
Zueva E.V.; St. Peterburg Pasteur Institute
Porin A.A.; St. Peterburg Pasteur Institute; North-Western State Medical University named after I.I. Mechnikov
Rogacheva E.V.; St. Petersburg State Academy of Veterinary Medicine
Zheltakova I.R.; St. Peterburg Pasteur Institute
Khamitova I.V.; St. Peterburg Pasteur Institute
Timofeeva E.V.; Office of the Federal Service for Supervision of Consumer Rights Protection and Human Wellbeing in St. Petersburg
Bespalova G.I.; North-Western State Medical University named after I.I. Mechnikov
2018-05-17 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/674
трудовые мигранты; дифтерия; бруцеллез; лихорадка Ку; лептоспироз; парвовирусная инфекция; хеликобактерная инфекция; брюшной тиф
ru
Wide migration processes typical for megacities, including St. Petersburg, require a comprehensive study of the infection among migrants arriving on a work visa. Biological material for research was taken from 370 migrants who arrived in St. Petersburg on a work visa. The control group is represented by 320 adults of St. Petersburg. The methodology of the study of the biological material depended on the type of pathogen and included classical and modern methods of research. All obtained data are processed using adequate methods of mathematical statistics. C. diphtheriae strains in migrant workers were isolated 80 times more often than in permanent residents of St. Petersburg. In St. Petersburg gravis biovar occurs in 25% of cases, in the visiting contingent — in 83% of cases, which is an unfavorable prognostic sign. In migrants 17% of C. diphtheriae strains have a “silent” gene (tox+), which, under known conditions, can resume toxin production. The local people are protected from diphtheria by 95%, and labor migrant is only 66%. 17% of migrant workers with C. diphtheriae strains have a low level of protection against diphtheria, which poses a threat to them and those in contact with them. Infection with brucellosis pathogens of labor migrants from Uzbekistan is 9 times higher than the local population, persons from Tajikistan — 60 times higher. The infection rate of migrant workers from Uzbekistan and Tajikistan C. burnetii is 25 times higher than that of the local population. The chronic course of these infections complicates diagnosis and reduces the quality of life. According to the results of the screening test, S. Typhi bacterium carrier is distributed 7 times more in migrant workers from Uzbekistan and 2 times more in persons from Tajikistan than among the local population of St. Petersburg. The seroprevalence of toxic H. pylori in migrant workers is 84%, which is much higher than that of permanent residents of St. Petersburg (57%). The causes of this phenomenon have not been studied and require further study. Labor migrants from Central Asia have a low level of population immunity to parvovirus infection: 37% of seropositive persons from Uzbekistan and 62% from Tajikistan compared with 78% of the local population. This may contribute to the spread of parvovirus infection involving infection of seronegative residents of St. Petersburg risk groups, including blood donors, pregnant women, persons with immunodeficiencies, hematologic and oncologic patients. The results obtained ascertain the tense epidemiological situation among labour migrants in St. Petersburg for a number of infections. Reliable information will help to organize the correct further study of the problem and conduct appropriate measures to preserve the health of the local population and the visiting contingent.
oai:iimmun.ru:article/66
2022-04-12T21:20:02Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/66
2022-04-12T21:20:02Z
Russian Journal of Infection and Immunity
Vol 1, No 4 (2011); 331-340
APPLICATION OF MODERN STATISTICAL METHODS TO ASSESS THE INTERACTION OF THE INTERFERON STATUS OF FULL-TERM NEONATAL INFANT AND HIS MOTHER
Selkov S.A.; НИИ акушерства и гинекологии им. Д.О. Отта СЗО РАМН, Санкт-Петербург
Koroleva L.I.; НИИ акушерства и гинекологии им. Д.О. Отта СЗО РАМН, Санкт-Петербург
Tishkov A.V.; Санкт-Петербургский институт информатики и автоматизации РАН, Санкт-Петербург
Anikin V.B.; ФГБУ НИИ гриппа Минздравсоцразвития России, Санкт-Петербург
Khromov-Borisov N.N.; ГОУ ВПО СПбГМУ им. И.П. Павлова Росздрава, Санкт-Петербург
2014-07-01 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/66
интерфероновый статус; новорожденный; мать; статистический анализ
ru
Abstract. The analysis of the IFN-status of healthy full-term neonatal infants and their healthy mothers with modern statistical methods was conducted. It was established that the IFN status of healthy full-term neonatal infants differs from the same of their mothers with higher occurrence of IFNs and strengthened induced production of IFNα/β and IFNγ. The statistically significant interaction between indicators of the IFN status of newborns and their mothers has been revealed. The obtained data is important for assessment of nonspecific resistance in system mother–newborn.
oai:iimmun.ru:article/2004
2023-01-09T16:42:06Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/2004
2023-01-09T16:42:06Z
Russian Journal of Infection and Immunity
Vol 12, No 6 (2022); 1040-1050
Disseminated purulent peritonitis outcome affects NKT cell phenotype
https://iimmun.ru/iimm/article/download/2004/14013
https://iimmun.ru/iimm/article/download/2004/14014
https://iimmun.ru/iimm/article/download/2004/14015
https://iimmun.ru/iimm/article/download/2004/14016
https://iimmun.ru/iimm/article/download/2004/14017
https://iimmun.ru/iimm/article/download/2004/14018
https://iimmun.ru/iimm/article/download/2004/14019
https://iimmun.ru/iimm/article/download/2004/14020
https://iimmun.ru/iimm/article/download/2004/14021
https://iimmun.ru/iimm/article/download/2004/14022
https://iimmun.ru/iimm/article/download/2004/14023
https://iimmun.ru/iimm/article/download/2004/14922
Savchenko A.A.; Federal Research Center “Krasnoyarsk Science Center”, Siberian Branch of the Russian Academy of Sciences, Scientific Research Institute of Medical Problems of the North
Borisov A.G.; Federal Research Center “Krasnoyarsk Science Center”, Siberian Branch of the Russian Academy of Sciences, Scientific Research Institute of Medical Problems of the North
Kudryavtsev I.V.; I.P. Pavlov First St. Petersburg State Medical University, Russian Ministry of Health; Institute of Experimental Medicine
Belenjuk V.D.; Federal Research Center “Krasnoyarsk Science Center”, Siberian Branch of the Russian Academy of Sciences, Scientific Research Institute of Medical Problems of the North
2022-12-30 21:00:41
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
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Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/2004
перитонит; NKT-клетки; фенотип; исход заболевания; послеоперационный период; CD3–CD56+
Array
en
The aim of our study was to investigate the main characteristics of peripheral blood NKT cell phenotype in patients with disseminated purulent peritonitis (DPP) in dynamics of postoperative period, depending on the disease outcome. Fifty-two patients with acute surgical diseases and injuries of the abdominal organs complicated by DPP, and 68 healthy individuals in control group, were examined. Blood sampling was performed before surgery (preoperative period), as well as on the day 7, 14 and 21 of postoperative period. All patients with DPP were divided into two groups depending on disease outcome in postoperative period: patients with favorable disease outcome (n = 34); and patients with unfavorable outcome (n = 18). Study of the phenotype of blood NKT lymphocytes was performed by flow cytometry using direct immunofluorescence of whole peripheral blood samples with monoclonal antibodies. The low relative and absolute level of NKT cells was observed in DPP patients regardless of outcome disease in preoperative period. At the same time, the absolute level of NKT cells returned to normal only in patients with favorable DPP outcome and only by day 21 after surgery. Patients with favorable DPP outcome by the end of examination period had normalized quantity of mature NKT-lymphocytes and significantly decreased level of cytotoxic cells which was apparently associated with migration of such cell subsets to site of inflammation. A reduced level of non-classical (expressing CD8 marker) mature and cytokine-producing NKT cells was detected only in patients with favorable DPP outcome in preoperative period which returned to normal by the end of postoperative period. At the same time, patients with unfavorable disease outcome had reduced quantity of NKT cells of these subsets by day 21 of postoperative treatment. Patients with favorable outcome had high level of mature and cytotoxic CD11b+ NKT cells already in the preoperative period, while patients with unfavorable DPP outcome had increased level of cytotoxic CD11b+ NKT cells only by day 21 after surgery. The proportion of NKT cells expressing activation markers (CD28 and CD57) was reduced in patients in preoperative period that returned to normal immediately after surgery with favorable outcome, while it recovered with unfavorable outcome closer to the end of postoperative examination. The defined features of NKT cell phenotype in patients with unfavorable DPP outcome characterize disturbances in subset ratio and mechanisms of functioning of this cell fraction. This determines a need to develop immunotherapeutic methods aimed at stimulating immunoregulatory activity of NKT cells.
oai:iimmun.ru:article/2032
2023-08-06T17:51:49Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/2032
2023-08-06T17:51:49Z
Russian Journal of Infection and Immunity
Vol 13, No 2 (2023); 309-318
Intrathecal nonspecific immunoglobulin synthesis in syphilitic infection
https://iimmun.ru/iimm/article/download/2032/14437
https://iimmun.ru/iimm/article/download/2032/14438
https://iimmun.ru/iimm/article/download/2032/14439
https://iimmun.ru/iimm/article/download/2032/14440
https://iimmun.ru/iimm/article/download/2032/14441
https://iimmun.ru/iimm/article/download/2032/14442
https://iimmun.ru/iimm/article/download/2032/14443
https://iimmun.ru/iimm/article/download/2032/73851
Ponomareva M.V.; Urals Institute of Dermatovenereology and Immunopathology
Levchik N.K.; Urals Institute of Dermatovenereology and Immunopathology
Zilberberg N.V.; Urals Institute of Dermatovenereology and Immunopathology
2023-04-24 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/2032
интратекальный синтез иммуноглобулинов; MRZ-реакция; нейросифилис; сифилис; антительный индекс; цереброспинальная жидкость; нейротропные вирусы
Array
ru
Intrathecal Ig synthesis is one of the characteristic laboratory features of neurosyphilis (caused by Treponema pallidum subspecies pallidum able to affect the central nervous system (CNS), but a persisting pathogen-driven immune response is problem of scar effect. This long-term Ig production within the CNS compartment even after timely and proper treatment remains unexplored. We hypothesized that a long-term current chronic inflammation in the CNS caused by syphilitic infection can be a trigger of nonspecific humoral immune response and the scar effect may result from non-specific B cell activation within the CNS in the absence of antigens. The MRZ reaction is a laboratory tool for detection of intrathecal nonspecific humoral immune reaction, which is poorly investigated in syphilis patient. The MRZ reaction (MRZR) is used as a marker of intrathecal nonspecific humoral immune response and is composed of the three antibody indices (AI) against neurotropic viruses: measles, rubella and varicella zoster virus. A positive MRZR, defined as an elevated AIs (> 1.5) against at least one or more viral agents, confirms diagnosis of a chronic or autoimmune disease involving CNS recently declared as a highly specific marker of multiple sclerosis. Insufficient data on neuroinfections motivated us to examine a hypothesis that a long-term chronic inflammation within the CNS compartment caused by syphilitic infection may be a trigger of intrathecal nonspecific humoral immune response. We assessed prevalence of positive MRZR in 147 patients with later-stage syphilis seropositive for MRZ viruses. Study group included 43 patients with first-time diagnosed neurosyphilis, 16 patients with a follow-up visit after neurosyphilis treatment, 88 patients with excluded neurosyphilis. There were no significant differences between categories of patients examined, and presence of positive MRZR was not associated with any demographic, clinical and laboratory characteristics. To sum up, our study (n = 147) showed that 27 patients (18%, 95% CI: 12–25% ) were positive for intrathecal Ig production against one, two or three of the M, R, Z viruses, and bi- and tri-specific MRZR was present in 7 of 147 patients (5%, 95% CI: 2–10%). The data obtained evidence that frequency of the MRZR for syphilitic infection matches that of found in general population and a long-term current syphilitic infection affecting CNS seems to not be a trigger of a nonspecific intrathecal immune response. Among our patients, we also selected 43 patients with previously treated syphilitic infection without any neurological symptoms and excluded neurosyphilis diagnosis, so these patients can be considered as an apparently healthy group.
oai:iimmun.ru:article/141
2022-04-12T21:20:53Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/141
2022-04-12T21:20:53Z
Russian Journal of Infection and Immunity
Vol 3, No 4 (2013); 327-334
ANALYSIS OF T-HELPER SUBSETS OF PERIPHERAL BLOOD OF PATIENTS WITH CHRONIC HEPATITIS C EXPRESSING CHEMOKINE RECEPTORS CXCR3 AND CCR6 AND ACTIVATION MARKERS CD38 AND HLA-DR
Elezov D.S.; St. Petersburg Pasteur Institute, St. Petersburg
Kudryavtsev I.V.; Institute of Experimental Medicine of the North West Branch of the Russian Academy of Medical Sciences, St. Petersburg; Far East Federal University, Vladivostok; St. Petersburg State University, St. Petersburg
Arsentieva N.A.; St. Petersburg Pasteur Institute, St. Petersburg
Semenov A.V.; St. Petersburg Pasteur Institute, St. Petersburg
Esaulenko E.V.; St. Petersburg State Pediatric Medical University, St. Petersburg
Basina V.V.; St. Petersburg State Pediatric Medical University, St. Petersburg
Totolian A.A.; St. Petersburg Pasteur Institute, St. Petersburg
2014-07-08 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/141
гепатит С; Т-хелперы; хемокиновые рецепторы; активационные маркеры; CXCR3; CCR6; CD38; HLA-DR
ru
Abstract. In case of hepatitis C the adequate adaptive immune response depends on T-helper lymphocyte subsets and recruiting specific T cells in the site of infection. The purpose of the study was to assess changes in subpopulations of T-helper cells bearing chemokine receptor CXCR3 and CCR6 and activation markers CD38 and HLA-DR in peripheral blood of patients with chronic hepatitis C (n = 19) and healthy donors (n = 32). The T-helper phenotype was assessed by flow cytometry using a combination of monoclonal antibodies HLA-DR-FITC/CD38-PE/CD3-ECD/CCR6-PE-Cy7/CXCR3-APC/CD4-APC-Cy7. It was demonstrated reduction of CD38+ subset, increase of CXCR3+ and CCR6+ subsets of T-helper cells in particular CD3+CD4+ CXCR3+CCR6+ cells and importance of joint determination of the chemokine receptors CXCR3 and CCR6 and the activation markers CD38 and HLA-DR compared to their separate analysis in chronic hepatitis C.
oai:iimmun.ru:article/328
2023-08-06T17:27:07Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/328
2023-08-06T17:27:07Z
Russian Journal of Infection and Immunity
Vol 5, No 3 (2015); 233-242
NEPHRITOGENIC ACTIVITY OF STREPTOCOCCUS PYOGENES emm1 AND emm12 GENOTYPES ISOLATED FROM PATIENTS AND ASYMPTOMATIC CARRIERS
Burova L.A.; Institute of Experimental Medicine, St. Petersburg, Russia
Pigarevsky P.V.; Institute of Experimental Medicine, St. Petersburg, Russia
Snegova V.A.; Institute of Experimental Medicine, St. Petersburg, Russia
Kuleshevich E.V.; Institute of Experimental Medicine, St. Petersburg, Russia
Zharkov D.A.; Military Medical Academy, St. Petersburg, Russia
Schalen C.; Lund University, Lund, Sweden
Totolian A.A.; Institute of Experimental Medicine, St. Petersburg, Russia
2015-11-30 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/328
постинфекционный гломерулонефрит;Streptococcus pyogenes;Fc-рецепция;IgG;иммунные комплексы
ru
In this paper the nephritogenic activity of Streptococcus pyogenes genotype emm1 and emm12 clinical isolates from scarlet fever patients and healthy children was considered. As earlier established, strains of these types differ in Fc-binding profile, interacting with native IgG and immune complexes (IC), respectively. As expected, all the type emm1 strains bound native IgG; besides, ICs interacted only with strains from patients but not with those from carriers. In contrast, all type emm12 strains appeared to be negative for native IgG, whereas ICs were bound by strains from patients exclusively. None of the tested strains bound IgG3. By immunization of rabbits, binding of native IgG as well as ICs was associated with increasing of anti-IgG antibodies titer, formation of ICs, «crescent» deposition of IgG and C3-complement, local production of the proinflammatory cytokine TNFα, аnd also with accumulation of lymphocytes in kidney tissue. These signs indicated immune inflammation, leading to experimental membrane-proliferative glomerulonephritis (PSGN). It is known that PSGN development depends on IC-binding by tissue FcγR, on complement activation as well as on tissue infiltration by macrophages/monocytes. According to the data of morphometric evaluation the nephritogenic activity of the type emm12 strains exceeded those of type emm1. On testing of three IC-binding emm12 strains in six rabbits, typical PSGN developed in 5 of them and an abortive process in 1 animal. In case of five IgG-binding type emm1 strains, out of ten rabbits full-blown PSGN was observed only in 3 of them, but abortive changes in 5 and negative result in 2 animals. No pathologic changes were elicited by the «carrier» strains of either genotype; the inability of these to bind ICs, according to literature data, could be explained by mutation in the Mga-regulator gene thereby impeding M-proteins synthesis. We conclude that isolation of type emm12 IC-binding strains at acute streptococcal infection should be considered a high risk-factor for postinfectious sequelae development. The rabbit model of PSGN used in this research thus allowed to reveal some main stages and features of its pathogenesis.
oai:iimmun.ru:article/1663
2023-11-16T16:55:23Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/1663
2023-11-16T16:55:23Z
Russian Journal of Infection and Immunity
Vol 12, No 1 (2022); 85-94
Assessing the 2019 rubella elimination status in the Russian Federation
https://iimmun.ru/iimm/article/download/1663/10216
https://iimmun.ru/iimm/article/download/1663/10217
https://iimmun.ru/iimm/article/download/1663/10218
https://iimmun.ru/iimm/article/download/1663/10219
https://iimmun.ru/iimm/article/download/1663/10220
https://iimmun.ru/iimm/article/download/1663/10221
https://iimmun.ru/iimm/article/download/1663/10222
https://iimmun.ru/iimm/article/download/1663/10223
https://iimmun.ru/iimm/article/download/1663/10224
https://iimmun.ru/iimm/article/download/1663/10225
https://iimmun.ru/iimm/article/download/1663/10226
https://iimmun.ru/iimm/article/download/1663/10227
https://iimmun.ru/iimm/article/download/1663/12155
Chekhlyaeva T.S.; G.N. Gabrichevsky Research Institute for Epidemiology and Microbiology
Tsvirkun O.V.; G.N. Gabrichevsky Research Institute for Epidemiology and Microbiology; The Peoples’ Friendship University of Russia
Turaeva N.V.; G.N. Gabrichevsky Research Institute for Epidemiology and Microbiology
Erokhov D.V.; G.N. Gabrichevsky Research Institute for Epidemiology and Microbiology
Barkinkhoeva L.A.; G.N. Gabrichevsky Research Institute for Epidemiology and Microbiology
Tikhonova N.T.; G.N. Gabrichevsky Research Institute for Epidemiology and Microbiology
2021-12-03 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1663
краснуха; элиминация; эпидемиология; надзор; генотип; вирус краснухи
Авторы выражают глубокую признательность сотрудникам управления Роспотребнадзора в Москве, Санкт-Петербурге, Оренбургской области, Республике Башкортостан, ФБУЗ Центр гигиены и эпидемиологии в Республике Башкортостан, ФБУЗ Центр гигиены и эпидемиологии в Оренбургской области, ФБУЗ Центр гигиены и эпидемиологии в Москве и ФБУН Санкт-Петербургский научно-исследовательский институт им. Пастера Роспотребнадзора за проведение эпидемиологического расследования и серологических исследований для лабораторного подтверждения случаев краснухи.
ru
In 2002, the WHO Regional Office for Europe developed the Strategic Program for the Prevention of Measles and Congenital Rubella Infections in the European Region, which was revised in 2004. As a result of the revision, an additional target was set to eliminate endemic rubella in the region by 2010. Rubella is a disease well controlled by vaccination that accounts for a theoretical potential to interrupt its global transmission. Since 2013, the Russian Federation has been implementing the National Rubella Elimination Program. Elimination criteria have been revised as the Program proceeds. Currently, the main criterion for rubella elimination is the absence of endemic (local) virus transmission for at least 36 months, which should be confirmed by molecular genetic research methods. In addition, in the Russian Federation, an incidence rate of less than 1 case per 1 million population is also used as one of the elimination criteria. Since 2013, due to a high (over 95%) coverage of preventive vaccinations a decrease in incidence rates and their stabilization at a level of less than 1 per 1 million population since 2014 state in favor of successfully implemented Program. Genetic monitoring of rubella virus strains circulating in human population noted the termination of endemic virus transmission. While implementing the Elimination Program, the prevailing virus genotypes that circulate in Russia were found to be genotypes 1E and 2B showing a global distribution. The data obtained after molecular genetic monitoring allowed to find that the strains isolated during this period belonged to different clusters accounting for in favor of being imported. Considering the above factors such as high vaccination coverage, low incidence rate and lack of endemic virus transmission, the 2017 WHO Committee on verification of measles and rubella elimination assigned the Russian Federation the status of a country that has achieved rubella elimination. The continuation of the phase of infection elimination is confirmed annually. This article presents the results on comprehensive assessment of rubella elimination status in the Russian Federation by specialists from the National Scientific and Methodological Center for Measles and Rubella and WHO EURO Moscow regional reference laboratory for measles and rubella based on 2019 epidemiological data and molecular genetic studies.
oai:iimmun.ru:article/1844
2023-08-06T17:08:48Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/1844
2023-08-06T17:08:48Z
Russian Journal of Infection and Immunity
Vol 12, No 3 (2022); 535-542
Evaluation of the two in-patient hospitals on potential environmental hazard during the period of new coronavirus infection in the Khabarovsk city (december 2020 — march 2021)
https://iimmun.ru/iimm/article/download/1844/12235
https://iimmun.ru/iimm/article/download/1844/12236
https://iimmun.ru/iimm/article/download/1844/12237
https://iimmun.ru/iimm/article/download/1844/12238
https://iimmun.ru/iimm/article/download/1844/12302
https://iimmun.ru/iimm/article/download/1844/12303
https://iimmun.ru/iimm/article/download/1844/12304
https://iimmun.ru/iimm/article/download/1844/12305
https://iimmun.ru/iimm/article/download/1844/12610
Trotsenko O.E.; Khabarovsk Research Institute of Epidemiology and Microbiology of the Federal Service for Surveillance on Consumers Rights Protection and Human Wellbeing (Rospotrebnadzor)
Bondarenko A.P.; Khabarovsk Research Institute of Epidemiology and Microbiology of the Federal Service for Surveillance on Consumers Rights Protection and Human Wellbeing (Rospotrebnadzor)
Pshenichnaya N.Y.; Central Research Institute of Epidemiology of the Federal Service for Surveillance on Consumers Rights Protection and Human Wellbeing (Rospotrebnadzor)
Zaitseva T.A.; Khabarovsk Krai Rospotrebnadzor Regional Office
Garbuz Y.A.; Center of Hygiene and Epidemiology of Khabarovsk Krai
Chishagorova I.V.; Center of Hygiene and Epidemiology of Khabarovsk Krai
Shmylenko V.A.; Khabarovsk Research Institute of Epidemiology and Microbiology of the Federal Service for Surveillance on Consumers Rights Protection and Human Wellbeing (Rospotrebnadzor)
Bazykina E.A.; Khabarovsk Research Institute of Epidemiology and Microbiology of the Federal Service for Surveillance on Consumers Rights Protection and Human Wellbeing (Rospotrebnadzor)
Ogienko O.N.; Khabarovsk Research Institute of Epidemiology and Microbiology of the Federal Service for Surveillance on Consumers Rights Protection and Human Wellbeing (Rospotrebnadzor)
2022-07-04 15:06:27
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1844
ЛПУ; больные; внебольничная пневмония; бактериальная флора; больничная среда; риск инфицирования
Array
ru
Microbiological monitoring after infectious diseases in the system of epidemiological surveillance implies simultaneous pathogen identification both among patients and in hospital environment. Our aim is to assess potential hospital environmental hazard for the two in-patient infectious disease hospitals of the Khabarovsk city by using bacteriological and epidemiological analysis during new coronavirus disease pandemic. Materials and methods. Bacteriological assessment of nasopharyngeal microflora in 241 patients suffering from community-acquired pneumonia that were hospitalized in the two prevention and treatment facilities of the Khabarovsk city was performed. Sanitary-bacteriological control of hospital environment (428 hospital environment samples and 91 air samples) was carried out in parallel. Bacteriological assessment was performed with classical methods. Identification of isolated bacteriological pathogens and evaluation of drug-resistant strains were carried out by utilizing bacteriological analyzer Vitek 2 Compact. Results. Nine different pathogens (Pseudomonas aeruginosa, Pseudomonas stutzeri, Acinetobacter baumannii, Klebsiella pneumoniae, Klebsiella oxytoca, Enterobacter cloacae, Pantoea, Enterococcus faecium, Staphylococcus haemolyticus) were isolated in 20 out of 428 samples — 4.7% [2.7–6.7]. Half of isolated agents — 2.3% [0.9–3.8] — were represented by drug-resistant isolates (10 out of 20 isolates) including 5 carbapenem-resistant isolates (Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae) and 5 isolates with multiple drug resistance (Enterobacter cloacae, Pantoea, Enterococcus faecium, Staphylococcus haemolyticus). Air samples contained pathogenic biological agents found in 6 out of 91 samples — 6.6% [1.5–11.7], and half of them — 3.3% [0.6–7.9] — were identified as drug-resistant variants, including S. aureus и S. haemolyticus. One of the surveyed hospitals was recognized as more hazardous due to microflora isolated from intensive care unit (A. baumannii and P. aeruginosa were resistant to 3rd–4th generation cephalosporins and carbapenems). Conclusion. Revealed circulation of wide range of microorganisms isolated from environment of two in-patient hospitals indicates high risk of healthcare-associated infections formation. Intensive care units can serve as a reservoir of healthcare-associated infections due to high percentage of patients with severe disease cases (“main reservoir” of drug-resistant strains).
oai:iimmun.ru:article/1342
2023-08-06T17:10:30Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/1342
2023-08-06T17:10:30Z
Russian Journal of Infection and Immunity
Vol 10, No 4 (2020); 729-734
Rise in 2017-2018 measles morbidity in Serbia and Northwest Russia
Stoiljkovic V.D.; Institute of Virology, Vaccine and Sera Torlak
Bichurina M.A.; St. Petersburg Pasteur Institute
Lavrentieva I.N.; St. Petersburg Pasteur Institute
Filipovic-Vignjevic S.B.; Institute of Virology, Vaccine and Sera Torlak
Bancevic M.D.; Institute of Virology, Vaccine and Sera Torlak
Zheleznova N.V.; St. Petersburg Pasteur Institute
Antipova A.Y.; St. Petersburg Pasteur Institute
2020-11-26 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1342
заболеваемость; корь; Сербия; Северо-Запад России; вакцинация; генотипы
en
In 2017, the WHO registered 23,927 measles cases in 44 out of 53 countries in the European region. In 2018, measles incidence rate increased up to 82,599 cases registered in 48 countries of the region, with a large number of measles-associated deaths. Overall, 72 measles fatalities were registered in 10 European countries, including Serbia (15 cases).Aim of the study: to characterize 2017—2018 epidemiological upsurge of measles incidence rate observed in the Republic of Serbia (RS) and the Northwestern Federal District (NWFD) of the Russian Federation.Materials and methods. During the 2017—2018 season, 944 serum samples were collected from patients with measles, rubella, or exanthematous diseases in the NWFD and tested in the Laboratory of Virology at the St. Petersburg Regional Centre for Measles Surveillance (SPbRC). In 2017—2018, 2,946 serum samples from the Republic of Serbia were analyzed in the SPbRC by using ELISA with IgM measles test system (Vector-Best, Russia; or Siemens, Germany). Urine and swab samples were examined by RT-PCR and used for isolation and genotyping of measles viruses.Results. From 2017 to 2018, 5,798 measles cases were registered in the RS, among which 2,946 were laboratory-confirmed (serological testing and/or PCR). Unvaccinated subjects or those with unknown vaccination status accounted for majority of the cases. Children under 5 years of age and adults aged 30 years and over dominated among measles patients. During this season, 15 deaths were reported. Several genotypes of measles virus circulated in the RS, e.g. В3 Dublin, D8 Gir Somnath, and D8 Herborn. In 2018, 109 measles cases were recorded in the NWFD, 5 of which were imported from abroad. Among patients, adults comprised 64.2%, wherein 74.3% were covered by unvaccinated subjects or those with unknown vaccination status. Rise in measles incidence rate linked to multiple importations of various measles virus genotypes: В3 Kabul; B3 Dublin; D8 Frankfurt; D8 Cambridge; and D8 Gir Somnath.
oai:iimmun.ru:article/1515
2022-04-12T15:58:29Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/1515
2022-04-12T15:58:29Z
Russian Journal of Infection and Immunity
Vol 11, No 4 (2021); 752-762
A relationship between causative agents of infectious diarrhea and fatal outcomes in pre-school children
https://iimmun.ru/iimm/article/download/1515/9129
https://iimmun.ru/iimm/article/download/1515/9132
https://iimmun.ru/iimm/article/download/1515/9133
https://iimmun.ru/iimm/article/download/1515/9134
https://iimmun.ru/iimm/article/download/1515/9135
https://iimmun.ru/iimm/article/download/1515/9136
https://iimmun.ru/iimm/article/download/1515/9137
https://iimmun.ru/iimm/article/download/1515/10460
https://iimmun.ru/iimm/article/download/1515/10461
https://iimmun.ru/iimm/article/download/1515/10462
https://iimmun.ru/iimm/article/download/1515/10467
https://iimmun.ru/iimm/article/download/1515/10827
Podkolzin A.T.; Central Research Institute of Epidemiology
Kozhakhmetova T.A.; Central Research Institute of Epidemiology
Kyasova D.K.; Central Research Institute of Epidemiology
Dalelova Z.K.; Central Research Institute of Epidemiology
Kuleshov K.V.; Central Research Institute of Epidemiology
Guseva A.N.; Central Research Institute of Epidemiology
Pavlova A.S.; Central Research Institute of Epidemiology
Veselova O.A.; Central Research Institute of Epidemiology
Parkina N.V.; Central Research Institute of Epidemiology
Konovalova T.A.; Central Research Institute of Epidemiology
Yatsyshina S.B.; Central Research Institute of Epidemiology
2021-05-13 00:00:00
Authors who publish with this journal agree to the following terms:
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Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1515
инфекционная диарея; дети; дифференциальная диагностика; летальный исход; ПЦР
ru
Infectious diarrhea is one of the leading causes of fatal outcomes in young children. Differential diagnostics of such infections within the first hours of illness poses significant objective obstacles. Data from laboratory studies of autopsy material and pathological studies provide valuable information for understanding the spectrum of differential diagnostics and etiological structure of infectious diarrhea with fatal outcomes in young children. Materials and methods. There were analyzed 100 cases of fatal outcomes in children under the age of six years registered in Russia from November 2011 to December 2019, who was diagnosed with infectious diarrhea at different levels of the healthcare system. The data were assessed based on available medical case reports and the laboratory testing of autopsy samples performed by using nucleic acid amplification methods. Results. The diagnosis of infectious diarrhea was revised in 24 patients, based on the data of a set of intravital and post-mortem studies. In patients with unconfirmed diagnosis of acute intestinal infections, pneumonia was the most often detected — in 45.8% (11/24), sepsis — in 29.2% (7/24), meningitis/meningoencephalitis, acute surgical pathology and asphyxiation associated with vomit aspiration — in 16.7 % (4/24) cases. The causative agents of infectious diarrhea were identified in 71 of 76 patients with confirmed diagnosis of acute intestinal infections. Most prevalent were group A rotaviruses — 52.6% (40/76), group F adenoviruses — 17.1% (13/76), and noroviruses — 13.2% (10/76). Combination of pathogens was detected in 29 cases (38.2%). Prehospital lethal outcomes in patients with infectious diarrhea were observed in 17 cases (22.4%). In total, rate of neonatal deaths due to acute intestinal infections accounted for 62.2% and 2-year-old toddlers — 20.3%. 64 of 76 (84%) children had no unfavorable premorbid background. The most common pathologies associated with infectious diarrhea with developing fatal outcomes were pneumonia (including aspiration pneumonia) in 22.4% (17/76) and aspiration asphyxia in 6.6% (5/76). Hemolytic-uremic syndrome associated with diarrhea was diagnosed in 7.9% (6/76) of children. Conclusions. Within the first years of life children comprise a risk group for developing fatal outcomes during infectious diarrhea. Lack of unfavorable premorbid background should not be considered as a reliable positive prognostic criterion. Diagnostics of pneumonia should be included in the mandatory examination plan for children with severe infectious diar rhea. Based on study of clinical and autopsy material, group A rotaviruses were the lead causative agents among those resulting in infectious diarrhea with fatal outcomes in young children. Special attention should be paid to preventing vomit aspiration within the first days after disease onset.
oai:iimmun.ru:article/185
2015-06-16T09:39:25Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/185
2015-06-16T09:39:25Z
Russian Journal of Infection and Immunity
Vol 4, No 3 (2014); 257-266
FLU IN CHILDREN AFTER A PANDEMIC IN ST. PETERSBURG GENERAL HOSPITAL
Dondurey E.A.; Research Institute of Influenza Ministry of healthcare of the Russian Federation. St. Petersburg
Sukhovetskaya V.F.; Research Institute of Influenza Ministry of healthcare of the Russian Federation. St. Petersburg
Osidak L.V.; Research Institute of Influenza Ministry of healthcare of the Russian Federation. St. Petersburg
Konovalova N.I.; Research Institute of Influenza Ministry of healthcare of the Russian Federation. St. Petersburg
Lobova T.G.; Research Institute of Influenza Ministry of healthcare of the Russian Federation. St. Petersburg
Pisareva M.M.; Research Institute of Influenza Ministry of healthcare of the Russian Federation. St. Petersburg
Gladchenko L.N.; N.F. Filatov Children’s City Clinical Hospital No. 5, St. Petersburg
Obraztsova E.V.; Research Institute of Influenza Ministry of healthcare of the Russian Federation. St. Petersburg
Gonchar V.V.; Research Institute of Influenza Ministry of healthcare of the Russian Federation. St. Petersburg
Minchenko S.I.; N.F. Filatov Children’s City Clinical Hospital No. 5, St. Petersburg
Korcheniuk L.V.; N.F. Filatov Children’s City Clinical Hospital No. 5, St. Petersburg
2014-08-15 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/185
постпандемический период; дети; многопрофильный стационар; надзор за гриппом; эволюционная изменчивость.
ru
Flu monitoring was carried out in children’s general hospital in St. Petersburg within three epidemic seasons (2010–2011, 2011–2012 and 2012–2013). 1916 patients under the age of 18 years were examined with the complex of virologic tests . The natural decreasing of flu incidence and predominant diagnosing in etiologic structure one of the virus serotypes [in the first A(H1N1)pdm09, in the second — A(H3N2)] have been observed during the first two years after a pandemic. In the third season restoration of the main characteristics of epidemic flu situation were detected: polietiology with annual change of serotypes proportions, late start (winter and spring), majority of younger children among hospitalaized patients and a mild course of disease. The most probable candidates [viruses of a subtype A(H3N2)] are revealed as a causal factor of significant increasing of disease incidence with the severe forms in the near future. Laboratory data were confirmed by increase in frequency of the complicated by pneumonia ARI at hospitalized patients during the periods of their maximum registration. All isolates received during the study were corresponded to the referens-strains included in vaccines. Thus, timely and appropriate vaccination during the studied period had to become an effective protection against a flu.
oai:iimmun.ru:article/384
2022-04-12T15:18:00Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/384
2022-04-12T15:18:00Z
Russian Journal of Infection and Immunity
Vol 6, No 1 (2016); 45-54
INTERLEUKIN 1 AND INTERLEUKIN 4 GENES POLYMORPHISM ASSOCIATED WITH EARLY AND PRESCHOOL AGE CHILDREN SENSITIZATION TO STREPTOCOCCUS PYOGENES
Shabaldin A.V.; Research Institute for Complex Issues of Cardiovascular Diseases, Kemerovo, Russian Federation
Shabaldina E.V.; Kemerovo State Medical Academy, Kemerovo, Russian Federation
Ryazantsev S.V.; St. Petersburg Research Institute of Ear, Throat, Nose and Speech, St. Petersburg, Russian Federation
Simbirtsev A.S.; Research Institute of Highly Pure Biopreparations, St. Petersburg, Russian Federation
2016-06-09 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/384
сенсибилизация к Streptococcus pyogenes;полиморфизм IL1B (+3953,С→T,rs 114634);полиморфизм IL1Ra (VNTR,intron 2,89 bp);полиморфизм IL4 (VNTR intron 3,70 bp)
ru
Background. Streptococcus pyogenes infection and sensitization to its antigens is considered to be an unfavorable factor for the induction of rheumatic pathology. The search for genetic predictors of rheumatic diseases in general, and sensitization to S. pyogenes, in particular, is of high relevance in modern medicine.Objective. To study the associations between interleukin 1 and interleukin 4 gene polymorphisms and the development of sensitization to antigens of S. pyogenes in toddlers and preschool children.Materials and methods. 771 children aged 2–6 years with recurrent acute respiratory tract infections treated by allergist-immunologist and otorhinolaryngologist were included in the study. Antibodies against S. pyogenes were determined by ELISA using commercial kits “Immunoteks” (Stavropol, Russia) in all children. Children with IgG immune response to S. pyogenes were assigned to the study group (n = 306), whereas children without this response were assigned to the control group (n = 465). Both groups underwent gene typing of IL1B (+3953, C→T, rs 114634), IL1Ra (VNTR, intron 2, 89 bp) and IL4 (VNTR intron 3, 70 bp) gene polymorphisms in the Laboratory for Pharmacogenomics, ICBFM SB RAS. The data were processed using routine statistical methods for genetic analysis and the statistical software package Statistica 6.0. There were no deviations from Hardy–Weinberg equilibrium across all loci, suggesting validity of association studies between individual and combined genotypes and sensitization to antigens of S. pyogenes.Results. Positive association between sensitization to antigens of S. pyogenes and individual genotypes have been found: IL1B (+3953, C→T)*C,T (52.6% in the study group vs. 39.8% in the control group, p = 0.001; OR = 2.02; CI(99%) 0.47–5.93), IL1Ra (VNTR, intron 2, 89 bp)*2r,5r (7.19% in the study group vs. 1.29% in the control group, p = 0.001; OR = 5.59; CI(99%) 1.58–19.77), IL4 (VNTR intron 3, 70 bp)*2r,2r (6.86% in the study group vs 3.01% in the control group, p = 0.05; OR = 2.34; CI(99%) 0.66–8.29); as well as for combined genotypes: IL1B (+3953, C→T)*C,C / IL1Ra (VNTR, intron 2, 89 bp)*2r,4r / IL4 (VNTR intron 3, 70 bp)*2r,2r (OR = 46.15); IL1B (+3953, C→T)*T,T / I-1Ra (VNTR, intron 2, 89 bp)*4r,4r / IL4 (VNTR intron 3, 70 bp)*2r,3r (OR = 8.82) and IL1B (+3953, C→T)*C,T / IL1Ra (VNTR, intron 2, 89 bp)*2r,2r / IL4 (VNTR intron 3, 70 bp)*3r,3r (OR = 7.23). Conclusion. High odds ratio (OR = 46.15) for IL1B (+3953, C→T)*C,C / IL1Ra (VNTR, intron 2, 89 bp)*2r,4r / IL4 (VNTR intron 3, 70 bp)*2r,2r suggests that combined genotype is a main marker of sensitivity and impaired immune tolerance to S. pyogenes in toddlers and preschool children. Thus, this study has confirmed the association between gene polymorphisms, and pro-inflammatory and proallergic cytokines, and autoimmune and allergic diseases.
oai:iimmun.ru:article/1596
2023-08-06T17:08:56Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/1596
2023-08-06T17:08:56Z
Russian Journal of Infection and Immunity
Vol 11, No 6 (2021); 1123-1130
Features of effector lymphocyte subsets in patients with uveal melanoma in recurrent and chronic herpesvirus infection
https://iimmun.ru/iimm/article/download/1596/9673
https://iimmun.ru/iimm/article/download/1596/9674
https://iimmun.ru/iimm/article/download/1596/9675
https://iimmun.ru/iimm/article/download/1596/9676
https://iimmun.ru/iimm/article/download/1596/9677
https://iimmun.ru/iimm/article/download/1596/9678
https://iimmun.ru/iimm/article/download/1596/9679
https://iimmun.ru/iimm/article/download/1596/9680
https://iimmun.ru/iimm/article/download/1596/9681
https://iimmun.ru/iimm/article/download/1596/9682
https://iimmun.ru/iimm/article/download/1596/9683
https://iimmun.ru/iimm/article/download/1596/9684
https://iimmun.ru/iimm/article/download/1596/11268
Balatskaya N.V.; Moscow Helmholtz Research Сentre of Eye Diseases
Saakyan S.V.; Moscow Helmholtz Research Сentre of Eye Diseases
Myakoshina E.B.; Moscow Helmholtz Research Сentre of Eye Diseases
Kulikova I.G.; Moscow Helmholtz Research Сentre of Eye Diseases
Krichevskaya G.I.; Moscow Helmholtz Research Сentre of Eye Diseases
2021-07-02 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1596
увеальная меланома; язва роговицы с вовлечением увеального тракта; субпопуляции лимфоцитов; герпесвирусная инфекция; иммунофенотипирование; иммунитет
ru
The aim of the study is to conduct a comparative analysis of percentages for peripheral blood effector lymphocyte subsets in patients with uveal melanoma manifested by recurrent and chronic herpesvirus infection. There were 141 subjects enrolled: 70 patients with uveal melanoma, 38 patients with corneal ulcers and involvement of the uveal tract as well as 33 healthy donors. Immunophenotyping was performed by using laser flow cytometry with panel of monoclonal antibodies to differentiate lymphocyte subpopulations. IgM and IgG antibodies to herpesvirus infections were determined by using enzyme-linked immunosorbent assay on an automatic ELISA analyzer Lazurit (USA) with diagnostic kits of CJSC “Vector-Best” (Koltsovo). The data obtained showed that the absolute number of blood lymphocytes (CD45+) in patients with uveal melanoma did not differ from those in healthy donors. In contrast, patients with corneal ulcers and involvement of the uveal tract had this parameter increased. A decreased relative and absolute count of T cells (CD3+) in uveal melanoma, but increased absolute CD3+ number in inflammation was observed. No difference in relative and absolute content of the CD3+CD4+ helper/inducer subpopulation in patients with recurrent herpesvirus infections was found. Corneal ulcers in cancer patients revealed significantly increased absolute level of CD3+CD4+ helpers/inductor cells. Chronic herpesvirus infection in uveal melanoma patients showed increased relative and absolute number of cytotoxic T lymphocytes (CD3+CD8+). Recurrent herpesvirus infection was featured with decreased relative number of T lymphocytes (CD3+CD8+), upon inflammation, there was noted increased absolute and decreased relative number compared with healthy subjects. Double positive T cells increased in tumor and inflammation. B lymphocytes (CD19+) increased in melanoma and inflammation. The relative number of blood natural killer cells (CD16+CD56+) in uveal melanoma increased upon recurrent infection. Inflammation was coupled to decreased relative level of natural killer cells (CD16+CD56+). Melanoma showed no changes in CD4+/ CD8+ ratio; upon inflammation, its increase was noted in acute and chronic herpesvirus infections (p < 0.05). The suppression of the immune system in uveal melanoma, restricting antiviral defense, was revealed. The data obtained seem to be important for development of personalized approaches to prognosis and treatment of patients with uveal melanoma.
oai:iimmun.ru:article/1851
2023-11-19T09:12:09Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/1851
2023-11-19T09:12:09Z
Russian Journal of Infection and Immunity
Vol 12, No 2 (2022); 271-278
Cytokine markers of clinical variants of infective endocarditis
https://iimmun.ru/iimm/article/download/1851/12308
https://iimmun.ru/iimm/article/download/1851/12309
https://iimmun.ru/iimm/article/download/1851/12310
https://iimmun.ru/iimm/article/download/1851/12311
https://iimmun.ru/iimm/article/download/1851/12312
https://iimmun.ru/iimm/article/download/1851/12313
https://iimmun.ru/iimm/article/download/1851/12314
https://iimmun.ru/iimm/article/download/1851/12315
https://iimmun.ru/iimm/article/download/1851/12316
https://iimmun.ru/iimm/article/download/1851/12317
https://iimmun.ru/iimm/article/download/1851/12318
https://iimmun.ru/iimm/article/download/1851/12319
https://iimmun.ru/iimm/article/download/1851/12320
https://iimmun.ru/iimm/article/download/1851/12321
https://iimmun.ru/iimm/article/download/1851/12983
https://iimmun.ru/iimm/article/download/1851/13369
https://iimmun.ru/iimm/article/download/1851/13370
https://iimmun.ru/iimm/article/download/1851/13371
https://iimmun.ru/iimm/article/download/1851/13372
https://iimmun.ru/iimm/article/download/1851/13373
https://iimmun.ru/iimm/article/download/1851/13374
Samoylenko E.S.; Kuban State Medical University; Scientific Research Institute – Ochapovsky Regional Clinical Hospital №1
Kolesnikova N.V.; Kuban State Medical University
Podsadnyaya A.A.; Specialized Clinical Infectious Diseases Hospital
Bratova A.V.; Scientific Research Institute – Ochapovsky Regional Clinical Hospital №1
2022-05-13 14:39:08
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1851
инфекционный эндокардит; цитокины; интерлейкины; патогенез; диагностика; тромбоэмболические осложнения
Array
ru
Introduction. Infective endocarditis is a bacterial disease. Pathogen is localized mainly on heart valves and endocardium. This condition is accompanied by immunopathological manifestations and potential generation of septic process being unfavorable prognosis for disease outcome. Currently, the causes of death of patients with endocarditis have been increasingly presented as thromboembolic complications, which severity depends on variant of the disease course. An important role in imbalanced immune system in the infectious process is assigned to altered intercellular interaction mediated via cytokine network. Activated immune cells produce cytokines, which investigating is important in terms of interpreting changes in immune system functionality, assessing the severity of diseases and controlling therapeutic effectiveness. Infective endocarditis remains a severe disease associated with high mortality despite current advances in diagnostics and treatment. A timely diagnostic process and early initiation of treatment are major factors for successful patient management necessitating to improve diagnostics of various clinical variants of endocarditis course, taking into account pathogenetically relevant cytokines. Objective was to comparatively evaluate the importance of serum cytokine concentrations in patients with uncomplicated course of infective endocarditis and its thromboembolic complications and determine cytokine markers of various variants of endocarditis course. Materials and methods. An immunological examination of 119 blood serum samples from patients with confirmed diagnosis of infectious endocarditis and 20 samples from apparently healthy persons was carried out. Depending on the clinical disease form, the patients were divided into 4 groups: group 1 — primary infective endocarditis (PIE) with thromboembolic complications (n = 24), 2 — PIE without thromboembolic complications (n = 34), 3 — secondary IE with thromboembolic complications (n = 27), 4 — secondary IE without thromboembolic complications (n = 34). The control group consisted of 20 apparently healthy subjects. Immunological studies of serum cytokine concentrations were conducted in all groups. Results. Statistically significant increase in serum concentration of IL-10, IL-6, VEGF-A, IL-18, IL-1Ra and IL-8 was revealed in all clinical groups of patients compared to those in control group (p < 0,05). By correlation analysis, we found a significant positive relationship between IL-10 and IL-18 or IL-6. An increase in the concentration of IL-10 leads to increased level of pro-inflammatory IL-18 and IL-6. The marker of secondary endocarditis was observed as increased level of serum concentrations of IFNγ. A characteristic feature of primary infective endocarditis with thromboembolic complications was revealed as significantly increased serum concentration of IL-8, IL-1Ra and IL-6. Markers of secondary complicated endocarditis were identified as increased level of IL-6, VEGF-A and IL-18.
oai:iimmun.ru:article/1232
2023-08-06T17:10:46Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/1232
2023-08-06T17:10:46Z
Russian Journal of Infection and Immunity
Vol 10, No 3 (2020); 506-514
Lung memory T-cell response in mice following intranasal immunization with influenza vector expressing mycobacterial proteins
Shurygina A.S.; Smorodintsev Research Institute of Influenza Russian Ministry of Health
Zabolotnykh N.V.; Saint-Petersburg State Research Institute of Phthisiopulmonology Russian Ministry of Health
Vinogradova T.I.; Saint-Petersburg State Research Institute of Phthisiopulmonology Russian Ministry of Health
Vasilyev K.A.; Smorodintsev Research Institute of Influenza Russian Ministry of Health
Buzitskaya Z.V.; Smorodintsev Research Institute of Influenza Russian Ministry of Health
Stukova M.A.; Smorodintsev Research Institute of Influenza Russian Ministry of Health
2020-04-20 11:31:51
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1232
гриппозный вектор; ESAT-6; Ag85A; вакцина БЦЖ; Т-клетки памяти; проточная цитометрия
ru
Improving specific prevention of tuberculosis continues to be a top priority in phthisiology. “Prime-boost” vaccination schemes aim to maintain adequate levels of specific immunity while forming long-term protection. They are based on sequential use of BCG vaccine and new vaccine candidates expressing protective mycobacterial proteins. The development of new tuberculosis prevention approaches requires an understanding of how the anti-tuberculosis immune response forms and which mechanisms provide TB protection. Since tuberculosis is an airborne infection, vaccine effectiveness largely depends on mucosal immunity based on the formation of long-lived, functionally-active memory T-lymphocytes in the respiratory tract. We have previously shown that the influenza vector expressing ESAT-6 and Ag85A mycobacterial proteins (Flu/ESAT-6_Ag85A) in vaccination scheme of intranasal boost immunization resulted in significant increase of BCG's protective effect according to key indicators aggregate data in experimental tuberculosis infection. The aim of this work was to study the effect of intranasal immunization with the Flu/ESAT-6_Ag85A influenza vector on the formation of antigen-specific central and effector memory T cells and the cytokine-producing activity of effector T cells (TEM) in BCG standard and “BCG prime — influenza vector boost” vaccination schemes in mice. Intranasal immunization with the influenza vector has been shown to increase the proportion of antigen-specific CD4+ central memory T cells (TCM) in the pool of activated lymphocytes of lung and spleen reaching significant differences from the BCG group in the percentage of spleen CD4+ TCM (p < 0.01). In contrast to BCG, vaccination with the studied vaccine candidate was accompanied by accumulation of highly differentiated CD8 effector cells in lung, the target organ during tuberculosis infection. Comparative evaluation of the cell-mediated, post-vaccine immune response after immunization with influenzavector-based vaccine candidate (intranasal/mucosal) or BCG vaccine (subcutaneous) showed advantages in the mucosal group: in formation of functionally active subpopulations of effector CD4 and CD8 T lymphocytes (CD44highCD62Llow) in lungs secreting IL-2 as well as polyfunctional cells capable of coproducing two cytokines (IFNγ/TNFα or IFNγ/IL-2) or three cytokines (IFNγ/TNFα/IL-2). Due to their more pronounced effector function, polyfunctional T-lymphocytes can be considered to be potential immunological markers of protective immunity in tuberculosis.
oai:iimmun.ru:article/1424
2022-04-12T15:57:18Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/1424
2022-04-12T15:57:18Z
Russian Journal of Infection and Immunity
Vol 11, No 3 (2021); 530-538
Features of IgG-antibodies production to individual Cytomegalovirus proteins in various eye diseases (age-related macular degeneration and central serous chorioretinopathy)
https://iimmun.ru/iimm/article/download/1424/8174
https://iimmun.ru/iimm/article/download/1424/8175
https://iimmun.ru/iimm/article/download/1424/8176
https://iimmun.ru/iimm/article/download/1424/8177
https://iimmun.ru/iimm/article/download/1424/8178
https://iimmun.ru/iimm/article/download/1424/8179
https://iimmun.ru/iimm/article/download/1424/8180
https://iimmun.ru/iimm/article/download/1424/8181
https://iimmun.ru/iimm/article/download/1424/8182
https://iimmun.ru/iimm/article/download/1424/8183
https://iimmun.ru/iimm/article/download/1424/9932
Neroev V.V.; Helmholtz National Medical Research Center of Eye Diseases
Krichevskaya G.I.; Helmholtz National Medical Research Center of Eye Diseases
Alatortseva G.I.; I.I. Mechnikov Research Institute for Vaccines and Sera
Ryabina M.V.; Helmholtz National Medical Research Center of Eye Diseases
Sarygina A.P.; Helmholtz National Medical Research Center of Eye Diseases
Nesterenko L.N.; I.I. Mechnikov Research Institute for Vaccines and Sera
Dotsenko V.V.; I.I. Mechnikov Research Institute for Vaccines and Sera
Luhverchik L.N.; I.I. Mechnikov Research Institute for Vaccines and Sera
2020-08-23 19:30:37
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1424
цитомегаловирус, вирусные белки, антитела, линейный иммуноанализ, возрастная макулярная дегенерация, центральная серозная хориоретинопатия
ru
Age-related macular degeneration (AMD) and central serous chorioretinopathy (CSC) are diseases targeting the posterior segment of the eye that often lead to lowered visual functions. Pathogenesis of such disorders largely remains unclear. Among the risk factors of developing chronic inflammation, various microorganisms are considered, particularly Cytomegalovirus (CMV ). The study was aimed at analyzing the humoral response to individual viral proteins during chronic and reactivated CMV infection in AMD and CSC patients. Materials and methods. 104 CMV-seropositive patients were enrolled in the study including 75 AMD and 29 CSC subjects. IgM- and IgG-antibodies specific to CMV late viral antigens as well as IgG antibodies against the main non-structural immediate early (IE) antigen were evaluated by ELISA. IgG antibodies to individual CMV phosphoproteins such as the main non-structural immediate early protein (IE), the DNA-binding phosphoprotein pp52, and the tegument phosphoproteins (pp150, pp65, and pp28) were assessed by using Line-Immunoassay: recombinant antigens containing immunodominant protein fragments derived from viral antigens (p52, p150, p65, p28) were used. Positive (bands 2+) and strongly positive (bands 3+) data were only used for analysis. Results. It was shown that in both groups patients with chronic CMV infection had comparable rate of detected antibodies specific to individual antigens. The level of seropositivity to CMV р150 and р65 was significantly higher than that to CMV р52 and р28 (p < 0.05). Patients with AMD compared to patients with CSС had significantly higher moderate positive response (2+) to all the antigens examined. Upon reactivation of chronic CMV infection in AMD patients, the level of seropositivity to all antigens was increased, the number of cases with an intensely positive response to individual antigens was elevated, but patients with moderate positive response still prevailed. However, reactivation of chronic CMV infection was observed only in 6 CSС patients, allowing to perform no comparative analysis between these two groups. Conclusion. The main difference between CMV-chronically infected patients with AMD and CSC was not found at the level of seropositivity to individual CMV recombinant antigens, but rather in magnitude of antibody production so that AMD patients in comparison to CSC patients displayed moderate antibody production (bands 2+). A marked difference was related to the level of antibodies against CMV p150: AMD patients showed moderate antibody response (bands 2+), whereas CSC subjects dominated with strong positive response (bands 3+) (p < 0.05). It seems that moderate antibody production to recombinant CMV antigens examined in AMD patients occurs due to a weak expression of such viral antigens during chronic infection, resulting in long-term maintenance of antigenic stimulation leading to prolonged inflammation.
oai:iimmun.ru:article/620
2022-04-12T15:45:32Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/620
2022-04-12T15:45:32Z
Russian Journal of Infection and Immunity
Vol 9, No 2 (2019); 337-346
Assessing natural herd immunity to tick-borne encephalitis in Republic Sakha (Yakutia)
Dragomeretskaya A.G.; FBIS Khabarovsk Research Institute of Epidemiology and Microbiology
Ignatyeva M.E.; Central Office of the Federal Service for Surveillance on Costumer Rights Protection and Human Wellbeing, Republic Sakha (Yakutia)
Trotsenko O.E.; FBIS Khabarovsk Research Institute of Epidemiology and Microbiology
Mzhelskaya T.V.; FBIS Khabarovsk Research Institute of Epidemiology and Microbiology
Budatsirenova L.V.; Central Office of the Federal Service for Surveillance on Costumer Rights Protection and Human Wellbeing, Republic Sakha (Yakutia)
Grigoryeva V.I.; Central Office of the Federal Service for Surveillance on Costumer Rights Protection and Human Wellbeing, Republic Sakha (Yakutia)
Romanova A.P.; FBIS Khabarovsk Research Institute of Epidemiology and Microbiology
2019-05-13 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/620
клещевой вирусный энцефалит; Республика Саха (Якутия); неэндемичная территория; напряженность иммунитета; переносчики возбудителя
ru
Tick-borne encephalitis (TBE) is a natural focal viral neuroinfection vastly spread in the mid-latitude climate zone of theEurasia. Lethal cases and disability related to TBE determine its high epidemiological significance as well as underlie undiminished attention to prevent it in endemic territories. At the same time, TBE epidemiologic features significantly differ in various geographic areas. Importantly, Republic Sakha (Yakutia) is not among TBE endemic areas in theRussian Federation. However, in the last decade an increased incidence of ixodic tick bites was registered in the Republic Sakha. A pattern of pathogen-specific population immunity is one of the most valuable criteria for assessing magnitude of epidemic process in TBE foci. Hence, our study was aimed at assessing natural herd immunity TBEV in Republic Sakha associated with elevated incidence of tick bite visits. Here, we analyzed the data regarding the rate of tick attacks in the period of 2001–2007 in Republic Sakha. Residents and individuals unvaccinated against TBEV in the city ofYakutskas well as various administrative regions were examined to record the peak incidence of tick attacks. It was found that a range of the administrative regions recording with registered of ixodic tick attacks and elevated incidence of tick bites was expanded. Moreover, the top incidence of tick bites was annually recorded in the south regions of the republic Aldanian, Neryungri, Olyekminsk, Leninsk, Khangalassky districts, city ofYakutskand its suburbs. Furthermore, TBEV-specific immunoglobulins were detected in 5.7±0.68% of the residents examined suggesting about contacts with the pathogen. In addition, the peak count of seropositive people (8.9±1.85%) was identified in Namsky district located in the central part of the republic. Overall, detection rate of TBEV-specific antibodies among general adult population (6.9±0.95%) was significantly higher compared to those observed in children (3.9±0.89%, р < 0.05). no significant differences in urban (5.8±1.12%) vs. rural population (5.63±0.84%) was found.
oai:iimmun.ru:article/798
2023-08-06T17:11:56Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/798
2023-08-06T17:11:56Z
Russian Journal of Infection and Immunity
Vol 9, No 5-6 (2019); 735-742
Parameters of mineral metabolism and bone density in young males with <i>Helicobacter pylori</i>-associated chronic gastritis
https://iimmun.ru/iimm/article/download/798/4364
https://iimmun.ru/iimm/article/download/798/4365
https://iimmun.ru/iimm/article/download/798/4366
https://iimmun.ru/iimm/article/download/798/4367
https://iimmun.ru/iimm/article/download/798/4368
https://iimmun.ru/iimm/article/download/798/4369
https://iimmun.ru/iimm/article/download/798/4370
https://iimmun.ru/iimm/article/download/798/4371
https://iimmun.ru/iimm/article/download/798/4372
https://iimmun.ru/iimm/article/download/798/4373
https://iimmun.ru/iimm/article/download/798/4374
https://iimmun.ru/iimm/article/download/798/4375
https://iimmun.ru/iimm/article/download/798/4376
https://iimmun.ru/iimm/article/download/798/4377
https://iimmun.ru/iimm/article/download/798/4378
https://iimmun.ru/iimm/article/download/798/5844
https://iimmun.ru/iimm/article/download/798/5845
https://iimmun.ru/iimm/article/download/798/5846
https://iimmun.ru/iimm/article/download/798/5847
https://iimmun.ru/iimm/article/download/798/5848
https://iimmun.ru/iimm/article/download/798/5849
https://iimmun.ru/iimm/article/download/798/5850
https://iimmun.ru/iimm/article/download/798/5851
https://iimmun.ru/iimm/article/download/798/5852
https://iimmun.ru/iimm/article/download/798/5853
https://iimmun.ru/iimm/article/download/798/5854
https://iimmun.ru/iimm/article/download/798/5855
https://iimmun.ru/iimm/article/download/798/5856
https://iimmun.ru/iimm/article/download/798/5857
https://iimmun.ru/iimm/article/download/798/5901
Drygina L.B.; The Nikiforov Russian Center of Emergency and Radiation Medicine of the Ministry of Russian Federation for Civil Defense, Emergencies and Elimination of Consequences of Natural Disasters
Dorofeytchik-Drygina N.A.; The Nikiforov Russian Center of Emergency and Radiation Medicine of the Ministry of Russian Federation for Civil Defense, Emergencies and Elimination of Consequences of Natural Disasters
2020-01-30 21:56:34
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/798
инфекция H. pylori; низкая минеральная плотность костной ткани; атрофический гастрит; остеокальцин; дезоксипиридинолин; минеральный обмен
ru
Here we present the current data on the chronic gastritis prevalence in young people. An issue regarding involvement of Helicobacter pylori infection in formation of low bone density is discussed. Examining the features of bone tissue metabolism in infection-associated chronic gastritis to optimize diagnostic algorithm was shown to be of high significance. In the study there were enrolled 200 employees working at the EMERCOM of Russia, who suffered from the acid-dependent gastric diseases and risk factors for reducing bone mineral density. Depending on the bone mineral density parameters, examining functional activity of the gastric mucosa, verification of the H. pylori infection, examining parameters of mineral metabolism, vitamin D level, parathyroid hormone, bone tissue remodeling markers was performed. It was shown that atrophic changes in the gastric mucosa the played a lead role in developing low bone mineral density.
oai:iimmun.ru:article/2121
2023-11-19T06:05:37Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/2121
2023-11-19T06:05:37Z
Russian Journal of Infection and Immunity
Vol 13, No 3 (2023); 481-496
Molecular genetic characterization of the epstein–Barr Virus: a relationship with the clinical features of pediatric infectious mononucleosis
https://iimmun.ru/iimm/article/download/2121/15409
https://iimmun.ru/iimm/article/download/2121/15410
https://iimmun.ru/iimm/article/download/2121/15412
https://iimmun.ru/iimm/article/download/2121/15413
https://iimmun.ru/iimm/article/download/2121/15414
https://iimmun.ru/iimm/article/download/2121/15415
https://iimmun.ru/iimm/article/download/2121/15416
https://iimmun.ru/iimm/article/download/2121/15417
https://iimmun.ru/iimm/article/download/2121/15418
https://iimmun.ru/iimm/article/download/2121/73977
https://iimmun.ru/iimm/article/download/2121/73978
https://iimmun.ru/iimm/article/download/2121/73979
https://iimmun.ru/iimm/article/download/2121/73980
https://iimmun.ru/iimm/article/download/2121/73981
https://iimmun.ru/iimm/article/download/2121/77578
https://iimmun.ru/iimm/article/download/2121/77579
https://iimmun.ru/iimm/article/download/2121/78383
https://iimmun.ru/iimm/article/download/2121/78389
https://iimmun.ru/iimm/article/download/2121/139466
https://iimmun.ru/iimm/article/download/2121/139467
Popkova M.I.; Academician I.N. Blokhina Nizhny Novgorod Scientific Research Institute of Epidemiology and Microbiology of Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
Utkin O.V.; Academician I.N. Blokhina Nizhny Novgorod Scientific Research Institute of Epidemiology and Microbiology of Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
Filatova E.N.; Academician I.N. Blokhina Nizhny Novgorod Scientific Research Institute of Epidemiology and Microbiology of Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
Bryzgalova D.A.; Academician I.N. Blokhina Nizhny Novgorod Scientific Research Institute of Epidemiology and Microbiology of Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
Sakharnov N.A.; Academician I.N. Blokhina Nizhny Novgorod Scientific Research Institute of Epidemiology and Microbiology of Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
Soboleva E.A.; Nizhny Novgorod Regional Center for the Prevention and Control of AIDS and Infectious Diseases
Nazarova L.V.; Infectious Clinical Hospital No. 23
2023-06-26 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/2121
вирус Эпштейна–Барр; инфекционный мононуклеоз; ВЭБ-1; ВЭБ-2; LMP-1; секвенирование; ПЦР; геноварианты
Array
Отраслевая научно-исследовательская программа на 2021-2025 гг. «Научное обеспечение эпидемиологического надзора и санитарной охраны территории Российской Федерации. Создание новых технологий, средств и методов контроля и профилактики инфекционных и паразитарных болезней» (утв. приказом Роспотребнадзора от 24.12.2020 г. № 869, п. 1.3.4.1). Рег. № НИОКТР 121091400195-7
ru
Introduction. Infectious mononucleosis (IM) is an high priority viral infection in children. Epstein-Barr virus (EBV) is the main etiological agent of IM. EBV is classified into two main types — EBV-1 and EBV-2. In addition, different variants of the virus are isolated based on individual genes, among which the LMP-1 gene and the oncoprotein it encodes are the most well known. So far, the study of the clinical significance of EBV genetic diversity in EBV-IM in children in Russia has not been conducted. The aim of the study was to evaluate a relationship between EBV LMP-1 molecular genetic variants and clinical and laboratory manifestations of IM in children.
Materials and methods. The material of the study was presented by blood leukocyte and saliva samples of children aged 1–17 years with EBV-IM (n = 69). A total of 132 EBV isolates were studied. For differential detection of EBV-1/EBV-2, we used a previously optimized one-round PCR variant with electrophoretic detection of amplification products in agarose gel. The nucleotide sequences of the C-terminal region of the LMP-1 gene were determined by Sanger sequencing followed by analysis of the obtained sequences using the MEGA X software. Multiple Factor Analysis was used to search for the relationship between LMP-1 variants and clinical and laboratory manifestations of IM (32 signs and 8 groups of signs).
Results. It was established that only one type of virus, EBV-1, was identified in all children. At the same time, the severity of clinical manifestations of EBV-IM in children varied significantly (from 15.5 to 35.5 scores in total). Molecular genetic analysis of the sequences of the LMP-1 C-terminal region in Nizhny Novgorod region EBV isolates demonstrated a significant heterogeneity of the viral population, which was not limited only to their grouping according to known variants. According to the frequency of detection, B95-8 was the dominant variant of LMP-1 (60.6±6.0% of cases), other variants were less common (China 1, NC, Med– and China 1+В95-8). It was found that EBV-IM proceeded more easily and with less severity of the intoxication syndrome in cases of infection with a virus having the molecular genetic profile of EBV-1/B95-8, in particular EBV-1/B95-8/E214D. Conversely, EBV-1/Med–, as well as EBV-1/Med–/L338S, EBV-1/Med–/S229T, EBV-1/China 1/L338S and EBV-1/NC/S229T profiles were associated with more severe infection.
Conclusion. For the first time, the influence of the genetic diversity of EBV on the clinical manifestations of IM in children was revealed. In the context of the tasks to be solved in this study, it is necessary to conduct a larger-scale and systemic studies in different territories of Russia.
oai:iimmun.ru:article/17525
2024-02-17T14:59:08Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/17525
2024-02-17T14:59:08Z
Russian Journal of Infection and Immunity
Vol 13, No 6 (2023); 1018-1026
Optimized properties of live vaccine influenza reassortant strains obtained by reverse genetics
https://iimmun.ru/iimm/article/download/17525/157852
https://iimmun.ru/iimm/article/download/17525/157853
https://iimmun.ru/iimm/article/download/17525/157854
https://iimmun.ru/iimm/article/download/17525/157855
https://iimmun.ru/iimm/article/download/17525/157856
https://iimmun.ru/iimm/article/download/17525/157857
https://iimmun.ru/iimm/article/download/17525/157858
https://iimmun.ru/iimm/article/download/17525/157901
https://iimmun.ru/iimm/article/download/17525/157903
https://iimmun.ru/iimm/article/download/17525/157904
https://iimmun.ru/iimm/article/download/17525/157905
https://iimmun.ru/iimm/article/download/17525/157906
https://iimmun.ru/iimm/article/download/17525/157907
https://iimmun.ru/iimm/article/download/17525/157908
https://iimmun.ru/iimm/article/download/17525/158217
https://iimmun.ru/iimm/article/download/17525/158557
Larionova N.V.; Institute of Experimental Medicine
Kiseleva I.V.; Institute of Experimental Medicine
Bazhenova E.A.; Institute of Experimental Medicine
Stepanova E.A.; Institute of Experimental Medicine
Rudenko L.G.; Institute of Experimental Medicine
2023-12-25 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/17525
вирус гриппа; живая гриппозная вакцина (ЖГВ); холодоадаптированность (са-признак); температурочувствительность (ts-признак); аттенуация (att); классическая реассортация (NR); генно-инженерная сборка реассортантов (RGR)
Array
122020300200-8
ru
Classical reassortment in developing chicken eggs is a well-established technique for obtaining LAIV strains. Naturally generated reassortant vaccine strains are characterized by high reproductive capacity, genetically stable characteristics of temperature sensitivity and cold resistance, which correspond to the characteristics of the MDV involved in crossing with the epidemic virus. Along with antigenic relevance, natural reassortment ensures attenuation of vaccine strains, good reproduction capacity in upper respiratory tract cells and inability to reproduction in the lower respiratory tract. With classical reassortment, the speed and efficiency of obtaining vaccine reassortants largely depend on the properties of epidemic virus, and therefore cannot be stable. The potential of reverse genetics is attractive because it allows to obtain vaccine reassortants quickly and efficiently, reduce the likelihood of spontaneous mutations; however, the vaccine strain is deprived of the advantages of natural selection, in which the most viable clones are selected. This study presents the results of comparatively assessed A(H3N2) LAIVs obtained in parallel by classical reassortment and reverse genetics according to criteria confirming that vaccine strains inherit the necessary properties that guarantee their harmlessness and high reproduction in chicken embryos. Strains for LAIV obtained by both methods retained all attenuating mutations inherited from the MDV, were highly reproductive at the optimal temperature, with temperature sensitivity corresponded to the MDV. However, strains obtained by reverse genetics, was observed to have partial loss of cold resistance in comparison with that of the MDV and classical reassortants. Reduced cold adaptation may negatively affect vaccine effectiveness. It is important that after several additional passages in chicken embryos at low temperature, the cold resistance of the vaccine strain, assembled by reverse genetics, was increased. Credibly that cold resistance is a phenotypic trait, the degree of manifestation of which depends on the temperature conditions of virus multiplication. The selective factor of reduced incubation temperature is missing in reverse genetics. In order for the cold-adapted phenotype to be fully realized, additional passages at low temperature of RG-reassortants are necessary. Thus, the reverse genetics method using plasmid technology allows to effectively prepare reassortant strains for LAIV. An important stage in obtaining vaccine strains using genetic engineering techniques should be the control of their cold-adapted phenotype and its optimization by additional passages at low temperature.
oai:iimmun.ru:article/598
2022-04-12T15:21:10Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/598
2022-04-12T15:21:10Z
Russian Journal of Infection and Immunity
Vol 7, No 4 (2017); 405-408
THE STUDY OF INFLUENZA VIRUS NEURAMINIDASE HYDRATION DEGREE
Grebenkina N.S.; I.I. Mechnikov Research Institute of Vaccines and Sera, Moscow
Kontarov N.A.; I.I. Mechnikov Research Institute of Vaccines and Sera, Moscow
Yuminova N.V.; I.I. Mechnikov Research Institute of Vaccines and Sera, Moscow
2018-01-20 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/598
степень гидратации; нейраминидаза; модель адсорбции Брунауэра–Эммета–Теллера; иммуногенность вируса гриппа
ru
It is known that the functioning of many proteins and enzymes depends on the degree of hydration of their surfaces. In our studies, neuraminidase (NA) of influenza virus was selected as a model for surface antigenic viral protein. The Brunauer–Emmett–Teller (BET) model of adsorption was used to calculate the values of water monolayer (am) at different values of water vapor pressure. The obtained BET isotherms allow for concluding that hysteresis takes place manifested by the difference between the monolayer am values for sorption and desorption of water from the surface of the enzyme, which is probably associated with a high degree of cooperation of the hydration shell formed. The maximum binding of water molecules was observed for the vapor pressure p/ps value of 0.65 and was am = 224 water molecules per a molecule of the enzyme. Basing on the calculated surface area of a NA tetramer (S = 256 nm2 ) and the maximum projection area of water molecule, it may be concluded that the entire surface of the enzyme is completely covered with a water monolayer. For said am value the maximum activity of NA was observed, whereas the minimum enzyme activity corresponded to the am value of 98 water molecules per a molecule of the enzyme, which corresponded to the water vapor pressure p/pS value of 0.38. Thus, for the influenza virus NA protein a dependency of the enzymatic activity on the degree of hydration of the surface of the enzyme is demonstrated. The dependence of immunogenicity of influenza virus from the extent of hydration of NA.
oai:iimmun.ru:article/53
2022-04-12T21:19:52Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/53
2022-04-12T21:19:52Z
Russian Journal of Infection and Immunity
Vol 1, No 3 (2011); 249-254
THE DYNAMICS OF TITERS CHANGES OF SPECIFIC IMMUNOGLOBULINS TO HBsAg IN SERUM AND YOLK OF QUAIL EGGS IN CASE OF USING OF DIFFERENT PREPARATION FOR IMMUNIZATION
Sboychakov V.B.; Федеральное государственное военное образовательное учреждение высшего профессионального образования «Военно-медицинская академия имени С.М. Кирова»
Borisenko S.V.; Государственное научное учреждение «Всероссийский научно-исследовательский ветеринарный институт птицеводства Российской академии сельскохозяйственных наук»
Sokurova A.M.; Федеральное государственное военное образовательное учреждение высшего профессионального образования «Военно-медицинская академия имени С.М. Кирова»
2014-06-30 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/53
HBsAg; специфические иммуноглобулины; титр; перепел
ru
Abstract. The special study to find a more cost-effective source of immunodiagnostic products was conducted. Determination of titers of specific immunoglobulins to HBsAg in serum and egg yolk of quail using different preparation for immunization showed that the most effective mode is immunization of quail by the HBsAg adsorbed on aluminum hydroxide and injected into reverse emulsion «water–oil».
oai:iimmun.ru:article/767
2023-08-06T17:12:44Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/767
2023-08-06T17:12:44Z
Russian Journal of Infection and Immunity
Vol 9, No 1 (2019); 173-182
In vivo virulence of Beijing genotype Mycobacterium tuberculosis
https://iimmun.ru/iimm/article/download/767/4229
https://iimmun.ru/iimm/article/download/767/4230
https://iimmun.ru/iimm/article/download/767/4231
https://iimmun.ru/iimm/article/download/767/4232
https://iimmun.ru/iimm/article/download/767/4233
https://iimmun.ru/iimm/article/download/767/4234
https://iimmun.ru/iimm/article/download/767/4235
https://iimmun.ru/iimm/article/download/767/4236
https://iimmun.ru/iimm/article/download/767/4237
https://iimmun.ru/iimm/article/download/767/4238
https://iimmun.ru/iimm/article/download/767/5604
Bespyatykh J.A.; Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency
Vinogradova Т.I.; St. Petersburg Research Institute of Phthisiopulmonology
Manicheva O.A.; St. Petersburg Research Institute of Phthisiopulmonology
Zabolotnykh N.V.; St. Petersburg Research Institute of Phthisiopulmonology
Dogonadze M.Z.; St. Petersburg Research Institute of Phthisiopulmonology
Vitovskaya M.L.; St. Petersburg Research Institute of Phthisiopulmonology
Guliaev A.S.; Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency
Zhuravlev V.Y.; St. Petersburg Research Institute of Phthisiopulmonology
Shitikov E.A.; Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency
Ilina E.N.; Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency
2019-03-21 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/767
туберкулез; вирулентность; Mycobacterium tuberculosis; микобактерии; мышиные модели; Beijing B0/W14
Работа выполнена при финансовой поддержке Российского научного фонда (№ 17-15-01412)
ru
Mycobacterium tuberculosis Beijing genotype strains comprise 50–80% in Russian Federation, which are divided into the main B0/W148, CladeA, and CAO clusters based on VNTR and SNP analysis. It should be noted that such phylogenetically highly close MTB strains belong to the modern Beijing family, generally demonstrating high transmissibility, association with drug resistance, and prevalence among patients with severe forms of the disease. However, studies on MTB genetic cluster strain-related virulence are scarce and contradictory. Here, we investigated virulence of diverse Mycobacterium tuberculosis strains belonging to the B0/W148, CladeA and CAO clusters and nonclustered strain NK of the Beijing family as well as laboratory strain H37Rv in C57BL/6 mice. It was found that mice infected with NK and B0/W148 vs. CladeA strains revealed the peak and the lowest mortality, respectively, while assessing survival rate in various groups (20 mice per MTB strain examined). Analyzing experimental data in mice demonstrated that all MTB strains were able to cause typical tuberculosis-related pathogenic signs. In particular, time-dependent evaluation of pathological changes (on 1, 3, 7, 14, 21, 28, 60 and 120 day post infection) in the lungs and spleen revealed significant differences among various strains. Tuberculosis progression was observed in the mice infected with B0/W148 and NK strains, whereas CladeA, CAO and H37Rv strains resulted in stabilized course and less marked organs damage. Moreover, we found that bacterial load after infection with Beijing family clustering strains was lower compared to that of the reference H37Rv strain, except NK strain demonstrating the peak bacterial load among the Beijing family comparable to H37Rv strain at 120 dpi. Thus, it was found that the level of virulence between most virulent B0/W148 cluster strain vs. NK strain was similar. Overall, the data obtained indicate that Beijing genotype strains are characterized by a diverse range of phenotypic virulence in vivo.
oai:iimmun.ru:article/1931
2023-01-19T14:31:50Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/1931
2023-01-19T14:31:50Z
Russian Journal of Infection and Immunity
Vol 12, No 5 (2022); 929-937
<i>CDR1, CDR2, MDR1</i> and <i>ERG11</i> expression in azole resistant <i>Сandida albicans</i> isolated from HIV-infected patients in city of Moscow
https://iimmun.ru/iimm/article/download/1931/13116
https://iimmun.ru/iimm/article/download/1931/13117
https://iimmun.ru/iimm/article/download/1931/13119
https://iimmun.ru/iimm/article/download/1931/13120
https://iimmun.ru/iimm/article/download/1931/13121
https://iimmun.ru/iimm/article/download/1931/13122
https://iimmun.ru/iimm/article/download/1931/13123
https://iimmun.ru/iimm/article/download/1931/13124
https://iimmun.ru/iimm/article/download/1931/13143
https://iimmun.ru/iimm/article/download/1931/13144
https://iimmun.ru/iimm/article/download/1931/13716
https://iimmun.ru/iimm/article/download/1931/14892
https://iimmun.ru/iimm/article/download/1931/14893
Voropaev A.D.; I.M. Sechenov First Moscow State Medical University (Sechenov University)
Yekaterinchev D.A.; I.M. Sechenov First Moscow State Medical University (Sechenov University)
Urban Y.N.; G.N. Gabrichevsky Research Institute for Epidemiology and Microbiology
Zverev V.V.; I.M. Sechenov First Moscow State Medical University (Sechenov University)
Nesvizhsky Y.V.; I.M. Sechenov First Moscow State Medical University (Sechenov University)
Voropaeva E.A.; G.N. Gabrichevsky Research Institute for Epidemiology and Microbiology
Likhanskaya E.I.; G.N. Gabrichevsky Research Institute for Epidemiology and Microbiology
Afanasiev M.S.; I.M. Sechenov First Moscow State Medical University (Sechenov University)
Afanasiev S.S.; G.N. Gabrichevsky Research Institute for Epidemiology and Microbiologyor epidemiology and microbiology
2022-11-16 17:27:43
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1931
Candida albicans; ВИЧ; флуконазол; ERG11; CDR1; CDR2; MDR1
Array
121021000287-0
ru
Candida fungi are common opportunistic microorganisms capable of causing infections of various localization, as well as life-threatening conditions in immunocompromised patients, such as HIV-infected individuals, oncology patients, subjects undergoing HSCT, which number has been steadily increasing in recent years. In addition, resistance to anti-fungal drugs has been spreading as well. Naturally sensitive to azoles, C. albicans possess a variety of mechanisms of acquired resistance, including efflux transporters and target protein-encoding gene amplification. This study was conducted to assess a prevalence of such mechanisms in the isolates sample obtained from HIV-infected patients in the Moscow region of the Russian Federation, characterize a relationship between these mechanisms and patterns of developing drug resistance. 18 strains of C. albicans resistant to fluconazole and voriconazole were isolated from HIV-infected patients with recurrent oropharyngeal candidiasis in the Moscow region. The expression levels of the ERG11, MDR1, CDR1, CDR2 genes involved in the formation of acquired azole resistance were measured using quantitative PCR, the –2ΔΔCT method with ACT and PMA genes as control genes and reference values of sensitive isolates. Expression levels exceeding the average values of sensitive isolates by more than 3 standard deviations were considered significantly elevated. In most of the isolates, elevated levels of CDR1 and CDR2 gene expression were found: 89% and 78%, respectively. The expression level of the MDR1 gene was increased only in 28% of cases. ERG11 expression levels were significantly elevated in 78% of the isolates. Expression levels of all resistance genes studied were significantly increased in 4 strains. In this sample of C. albicans isolates, acquired resistance is mainly associated with efflux vectors encoded by the CDR1 and CDR2 genes. Also, in most isolates, an increased expression level for the azole target protein gene — ERG11 was detected. The expression level of the efflux transporter gene MDR1 was increased in the smallest number of samples. It is also impossible to exclude a potential role of other mechanisms in developing acquired resistance, such as mutations in the ERG11 gene. It can be assumed that the identified mechanisms of resistance result from long-term, widespread, and sometimes uncontrolled use of azoles, including those in treatment and prevention of candidiasis in HIV-infected patients.
oai:iimmun.ru:article/2047
2023-08-06T17:51:49Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/2047
2023-08-06T17:51:49Z
Russian Journal of Infection and Immunity
Vol 13, No 2 (2023); 243-256
Morphostructural damage to bacterial cells exposed to chlorine-containing derivatives of 5-,6-,7-aminoindoles assessed by scanning electron microscope
https://iimmun.ru/iimm/article/download/2047/14991
https://iimmun.ru/iimm/article/download/2047/14992
https://iimmun.ru/iimm/article/download/2047/14993
https://iimmun.ru/iimm/article/download/2047/14994
https://iimmun.ru/iimm/article/download/2047/14995
https://iimmun.ru/iimm/article/download/2047/14996
https://iimmun.ru/iimm/article/download/2047/14997
https://iimmun.ru/iimm/article/download/2047/14998
https://iimmun.ru/iimm/article/download/2047/14999
https://iimmun.ru/iimm/article/download/2047/15000
https://iimmun.ru/iimm/article/download/2047/15001
https://iimmun.ru/iimm/article/download/2047/15002
https://iimmun.ru/iimm/article/download/2047/15003
https://iimmun.ru/iimm/article/download/2047/15004
https://iimmun.ru/iimm/article/download/2047/15005
https://iimmun.ru/iimm/article/download/2047/15006
https://iimmun.ru/iimm/article/download/2047/15007
https://iimmun.ru/iimm/article/download/2047/15008
https://iimmun.ru/iimm/article/download/2047/15012
https://iimmun.ru/iimm/article/download/2047/15013
https://iimmun.ru/iimm/article/download/2047/15014
https://iimmun.ru/iimm/article/download/2047/15015
https://iimmun.ru/iimm/article/download/2047/15016
https://iimmun.ru/iimm/article/download/2047/15017
https://iimmun.ru/iimm/article/download/2047/15018
https://iimmun.ru/iimm/article/download/2047/15019
https://iimmun.ru/iimm/article/download/2047/15020
https://iimmun.ru/iimm/article/download/2047/15021
https://iimmun.ru/iimm/article/download/2047/15022
https://iimmun.ru/iimm/article/download/2047/15023
https://iimmun.ru/iimm/article/download/2047/15024
https://iimmun.ru/iimm/article/download/2047/15025
https://iimmun.ru/iimm/article/download/2047/15026
https://iimmun.ru/iimm/article/download/2047/15027
https://iimmun.ru/iimm/article/download/2047/15028
https://iimmun.ru/iimm/article/download/2047/15029
https://iimmun.ru/iimm/article/download/2047/15030
https://iimmun.ru/iimm/article/download/2047/15031
https://iimmun.ru/iimm/article/download/2047/15032
https://iimmun.ru/iimm/article/download/2047/15033
https://iimmun.ru/iimm/article/download/2047/15034
https://iimmun.ru/iimm/article/download/2047/15035
https://iimmun.ru/iimm/article/download/2047/15036
https://iimmun.ru/iimm/article/download/2047/15037
https://iimmun.ru/iimm/article/download/2047/15038
https://iimmun.ru/iimm/article/download/2047/15039
https://iimmun.ru/iimm/article/download/2047/15040
https://iimmun.ru/iimm/article/download/2047/15041
https://iimmun.ru/iimm/article/download/2047/15043
https://iimmun.ru/iimm/article/download/2047/15044
https://iimmun.ru/iimm/article/download/2047/15045
https://iimmun.ru/iimm/article/download/2047/15046
https://iimmun.ru/iimm/article/download/2047/15047
https://iimmun.ru/iimm/article/download/2047/15048
https://iimmun.ru/iimm/article/download/2047/15049
https://iimmun.ru/iimm/article/download/2047/15050
https://iimmun.ru/iimm/article/download/2047/15051
https://iimmun.ru/iimm/article/download/2047/15052
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https://iimmun.ru/iimm/article/download/2047/15075
https://iimmun.ru/iimm/article/download/2047/15076
https://iimmun.ru/iimm/article/download/2047/15077
https://iimmun.ru/iimm/article/download/2047/15090
https://iimmun.ru/iimm/article/download/2047/109227
https://iimmun.ru/iimm/article/download/2047/109230
https://iimmun.ru/iimm/article/download/2047/109235
https://iimmun.ru/iimm/article/download/2047/109240
https://iimmun.ru/iimm/article/download/2047/109241
https://iimmun.ru/iimm/article/download/2047/109242
https://iimmun.ru/iimm/article/download/2047/109243
https://iimmun.ru/iimm/article/download/2047/109244
https://iimmun.ru/iimm/article/download/2047/109245
https://iimmun.ru/iimm/article/download/2047/109246
https://iimmun.ru/iimm/article/download/2047/109251
https://iimmun.ru/iimm/article/download/2047/109256
https://iimmun.ru/iimm/article/download/2047/109259
https://iimmun.ru/iimm/article/download/2047/109260
https://iimmun.ru/iimm/article/download/2047/109261
https://iimmun.ru/iimm/article/download/2047/109266
Maseykina A.A.; National Research Mordovia State University
Stepanenko I.S.; Volgograd State Medical University
Platkova T.N.; National Research Mordovia State University
Kiryutina A.I.; National Research Mordovia State University
Malysheva V.S.; National Research Mordovia State University
2023-04-24 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/2047
морфоструктурные изменения бактерий; хлорсодержащие производные 5-;6-;7-аминоиндолов; сканирующая электронная микроскопия; клеточная стенка; антимикробная активность; ESKAPE-патогены
Array
ru
The cell wall and membranes of Gram-positive and Gram-negative bacteria provide a physical, osmotic, and metabolic barrier between the internal contents of the bacterial cell and the external environment. Observation of changes in the integrity of the bacterial structure using a scanning electron microscope (SEM) can help elucidate the detailed mechanisms of cell death. The aim of the study was to analyze the morphological changes in microbial cells exposed to new compounds with antimicrobial activity — chlorine-containing derivatives of 5-,6-,7-aminoindoles using SEM. Methods. The present study was carried out using strains of Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli obtained from patients with nonspecific diseases of the respiratory, urinary tract, and intestines with different sensitivities to traditionally used antimicrobial drugs. Results. As a result, the studied chloromethyl-containing compounds of the indole series showed own biological activity, namely antimicrobial. Control cells were morphologically correct and typical. Statistical analysis of cell surface morphometry in control and experimental samples did not reveal significant changes in size after exposure to compounds with laboratory codes T1, T4, T7 and T12. At the same time, compared with control untreated cells of P. aeruginosa, S. aureus and E. coli, treatment with chlorine-substituted derivatives of 5-,6-,7-aminoindoles caused obvious morphological changes, which indicates a deteriorated state of the cell wall. Filamentous cells were observed in P. aeruginosa exposure to T7 and T12. The appearance of long filaments may be associated with the stress experienced by the cell after exposure to the compounds under study. It is believed that the formation of such filaments in bacteria under stress conditions results from defects in cell division, especially in the separation of daughter cells. There are data according to which, when DNA synthesis is suppressed, a bacterium changes its morphology, becomes longer, without reaching cell division. Treatment with T1, T7 and T12 resulted in degradation of the P. aeruginosa cell wall, while treatment with T4 caused the formation of pores on the cell surface. In this study, microscopy showed marked morphological changes in the cell walls of S. aureus, which led to deformation of the cell wall under the influence of T1, T4, T7 and T12. Treatment of E. coli T1, T4, T7 and T12 cells at a concentration of 500 μg/ml caused cell lysis, although normal cells were also found. The appearance of cellular debris around whole E. coli cells indicates membrane damage, which probably leads to a change in osmotic pressure. Conclusion. The results using SEM confirmed the data on the antimicrobial activity of chlorine-substituted derivatives of 5-,6-,7-aminoindoles.
oai:iimmun.ru:article/295
2023-08-06T17:27:17Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/295
2023-08-06T17:27:17Z
Russian Journal of Infection and Immunity
Vol 5, No 2 (2015); 143-147
THE CLINICAL AND LABORATORY EFFECTIVENESS OF OSELTAMIVIR FOR TREATMENT OF INFLUENZA IN HOSPITALIZED PATIENTS
Voloschcuk L.V.; Research Institute of Influenza Ministry of Healthcare of the Russian Federation
Golovacheva E.G.; Research Institute of Influenza Ministry of Healthcare of the Russian Federation
Go A.A.; Research Institute of Influenza Ministry of Healthcare of the Russian Federation
Mushkatina A.L.; Research Institute of Influenza Ministry of Healthcare of the Russian Federation
Zarishnuk P.V.; Research Institute of Influenza Ministry of Healthcare of the Russian Federation
Dnieprovskay G.L.; Research Institute of Influenza Ministry of Healthcare of the Russian Federation
Tumina T.L.; Research Institute of Influenza Ministry of Healthcare of the Russian Federation
2015-06-28 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/295
грипп;цитокины;интоксикация;катаральный синдром;озельтамивир;противовирусная активность
ru
The goal of our study was to estimate the efficacy of antiviral drug Oseltamivir for influenza treatment. We assessed the effect of Oseltamivir on the immune status of the 75 patients. It was formed 2 groups of observations with confirmed diagnosis of influenza: 38 received therapy Oseltamivir and 37 people receiving pathogenetic treatment. Treatment Oseltamivir contributes to a significant reduction in the duration of the catarrhal and intoxication syndromes and prevents the development of complications and exacerbations of chronic disease in persons with modified backdrop premorbid, which indicates for its high antiviral activity. We can assume the effect of Oseltamivir for restore the disturbed balance of cytokines, on assessing the dynamics of IL-1β, IL-8, IFNα, IFNγ in the serum.
oai:iimmun.ru:article/1610
2023-08-06T17:08:59Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/1610
2023-08-06T17:08:59Z
Russian Journal of Infection and Immunity
Vol 11, No 6 (2021); 1152-1158
Influence of ultraviolet blood irradiation on COVID-19 associated community-acquired pneumonia
https://iimmun.ru/iimm/article/download/1610/9792
https://iimmun.ru/iimm/article/download/1610/9793
https://iimmun.ru/iimm/article/download/1610/9794
https://iimmun.ru/iimm/article/download/1610/9795
https://iimmun.ru/iimm/article/download/1610/9796
https://iimmun.ru/iimm/article/download/1610/9797
https://iimmun.ru/iimm/article/download/1610/9798
https://iimmun.ru/iimm/article/download/1610/9799
https://iimmun.ru/iimm/article/download/1610/9800
https://iimmun.ru/iimm/article/download/1610/9801
https://iimmun.ru/iimm/article/download/1610/9888
https://iimmun.ru/iimm/article/download/1610/11960
https://iimmun.ru/iimm/article/download/1610/11968
Khodza I.E.; City Multiservice Hospital No. 2
Puzdryak P.D.; City Multiservice Hospital No. 2; North-Western State Medical University named after I.I. Mechnikov
Bondarenko P.B.; City Multiservice Hospital No. 2
Degtyarev A.M.; City Multiservice Hospital No. 2
Erofeev A.A.; City Multiservice Hospital No. 2
Chizhova K.A.; North-Western State Medical University named after I.I. Mechnikov
Shlomin V.V.; City Multiservice Hospital No. 2
Vedernikova E.S.; City Multiservice Hospital No. 2
2021-11-11 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1610
ультрафиолетовое облучение крови; новая коронавирусная инфекция; лечение COVID-19; внебольничная вирусная пневмония; пандемия XXI века
ru
The pandemic of the 21st century caused by the SARS-CoV-2 virus has posed a challenge for the global medical community. Community-acquired polysegmental pneumonia caused by the novel coronavirus infection (COVID-19) proceeds in a variety of types and may be complicated by a potentially lethal immune response — the cytokine storm. The latter is characterized by rapid proliferation and increased activity of T cells, macrophages and natural killer cells releasing various inflammatory cytokines and chemical mediators by protective cells. This pathological condition can be treated by recombinant humanized monoclonal antibody against monofunctional cytokine human interleukin-6 receptor. The effect of IL-6 blockers is to selectively bind and inhibit both soluble and membrane IL-6 receptors (sIL-6R and mIL-6R). There are studies demonstrating a positive effect and increased survival rate while using drugs that block the production of interleukin-6. The new coronavirus infection causes inflammation of the artery wall with intravascular thrombogenesis, which justifies the high efficiency of anticoagulant and hormone therapy. The standards of drug treatment of the studied infection include antiviral, anti-inflammatory, anticoagulant, mucolytic, symptomatic, intravenous infusion and oxygen therapy. Methodological recommendations for the diagnosis and treatment of a new coronavirus infection, issued by the Ministry of Health of the Russian Federation, are regularly updated in accordance with the accumulation of positive treatment results by global and local medical communities. In addition to drug therapy, there are other methods of body detoxification. One of the additional methods for treatment of community-acquired pneumonia along with viral “vasculitis” and correction of the immune response can be provided by ultraviolet blood irradiation (UBI). It is well known that ultraviolet radiation has a disinfecting effect. The wavelengths used in UBI affect the efficiency of UV absorption by DNA molecules of the pathogen. Bactericidal UV radiation at certain wavelengths causes thymine dimerization in DNA molecules. The accumulation of such changes in the DNA of microorganisms leads to a slowdown in the rate of their reproduction and extinction. The photohemocorrection method is characterized by immunostimulatory, antiinflammatory, anti-hypoxic, membrane stabilizing, antioxidant and detoxifying effects. In the current study we obtained data on a significant decrease in the systemic inflammatory response, marked and fast decrease of the C-reactive protein in blood tests of patients while receiving ultraviolet blood irradiation. The relief of the systemic inflammatory reaction had a positive effect on the reduction of infiltrative changes in the lung tissue, as well as the timing of discharge from hospital.
oai:iimmun.ru:article/1809
2023-08-06T17:08:34Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/1809
2023-08-06T17:08:34Z
Russian Journal of Infection and Immunity
Vol 12, No 3 (2022); 495-504
Comparison of the humoral and cellular immunity in COVID-19 convalescents
https://iimmun.ru/iimm/article/download/1809/11863
https://iimmun.ru/iimm/article/download/1809/11864
https://iimmun.ru/iimm/article/download/1809/11866
https://iimmun.ru/iimm/article/download/1809/11867
https://iimmun.ru/iimm/article/download/1809/11868
https://iimmun.ru/iimm/article/download/1809/11869
https://iimmun.ru/iimm/article/download/1809/11870
https://iimmun.ru/iimm/article/download/1809/11871
https://iimmun.ru/iimm/article/download/1809/11872
https://iimmun.ru/iimm/article/download/1809/11873
https://iimmun.ru/iimm/article/download/1809/12814
https://iimmun.ru/iimm/article/download/1809/13926
https://iimmun.ru/iimm/article/download/1809/13927
https://iimmun.ru/iimm/article/download/1809/13928
https://iimmun.ru/iimm/article/download/1809/13929
Toptygina A.P.; G.N. Gabrichevsky Research Institute for Epidemiology and Microbiology; Lomonosov Moscow State University
Semikina E.L.; National Medical Research Center of Children′s Health of the Ministry of Health of the Russian Federation; I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation
Zakirov R.S.; National Medical Research Center of Children′s Health of the Ministry of Health of the Russian Federation
Afridonova Z.E.; G.N. Gabrichevsky Research Institute for Epidemiology and Microbiology
2022-07-04 15:06:16
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1809
COVID-19; SARS-CoV-2; антитела; субклассы IgG; клеточный иммунитет; CD8+-лимфоциты
Array
ru
The SARS-CoV-2 virus caused the COVID-19 pandemic is related to the SARS-CoV-1 and MERS coronaviruses, which were resulted in 2003 and 2012 epidemics. Antibodies in patients with COVID-19 emerge 7–14 days after the onset of symptoms and gradually increase. Because the COVID-19 pandemic is still in progress, it is hard to say how long the immunological memory to the SARS-CoV-2 virus may be retained. The aim of this study was to study a ratio between humoral and cellular immunity against the SARS-CoV-2 S protein in COVID-19 convalescents. There were enrolled 60 adults with mild to moderate COVID-19 2 to 12 months prior to the examination. The control group consisted of 15 adults without COVID-19 or unvaccinated. Specific antibodies to the SARS-CoV-2 virus were determined by ELISA with the SARS-CoV-2-IgG-ELISA-BEST kit. To determine the specific IgG and IgA subclasses, the anti-IgG conjugate from the kit was replaced with a conjugate against the IgG subclasses and IgA. Additional incubation with or without denaturing urea solution was used to determine the avidity of antibodies. Peripheral blood mononuclear cells were isolated by gradient centrifugation, incubated with or without coronavirus S antigen for 20 hours, stained by fluorescently labeled antibodies, and the percentage of CD8highCD107a cells was assessed on flow cytometer BD FACSCanto II. In the control group, neither humoral nor cellular immunity against the SARS-CoV-2 S protein was found. In the group of convalescents, the level of IgG antibodies against the SARS-CoV-2 S protein varies greatly not being strictly associated with the disease duration, with 57% and 43% of COVID-19 patients having high vs. low level of humoral response, respectively. A correlation between level of specific IgG and IgA was r = 0.43. The avidity of antibodies increased over time in convalescents comprising 49.9% at 6–12 months afterwards. No virus-specific IgG2 and IgG4 subclasses were detected, and the percentage of IgG1 increased over time comprising 100% 6–12 months after recovery. 50% of the subjects examined had high cellular immunity, no correlations with the level of humoral immunity were found. We identified 4 combinations of humoral and cellular immunity against the SARS-CoV-2 S protein: high humoral and cellular, low humoral and cellular, high humoral and low cellular, and vice versa, low humoral and high cellular immunity.
oai:iimmun.ru:article/1366
2022-04-12T15:56:51Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/1366
2022-04-12T15:56:51Z
Russian Journal of Infection and Immunity
Vol 11, No 3 (2021); 539-548
Immunoglobulins and predicted mortality in clinical course of concomitant HIV and TB infection
https://iimmun.ru/iimm/article/download/1366/7565
https://iimmun.ru/iimm/article/download/1366/7566
https://iimmun.ru/iimm/article/download/1366/7567
https://iimmun.ru/iimm/article/download/1366/7568
https://iimmun.ru/iimm/article/download/1366/7569
https://iimmun.ru/iimm/article/download/1366/7570
https://iimmun.ru/iimm/article/download/1366/7571
https://iimmun.ru/iimm/article/download/1366/7572
https://iimmun.ru/iimm/article/download/1366/7573
https://iimmun.ru/iimm/article/download/1366/7574
https://iimmun.ru/iimm/article/download/1366/7575
https://iimmun.ru/iimm/article/download/1366/9964
Mal’tseva N.V.; Novokuznetsk State Institute for Further Training of Physicians, Branch Campus of the FSBEI FPE RMACPE MOH
Victorova I.B.; Novokuznetsk State Institute for Further Training of Physicians, Branch Campus of the FSBEI FPE RMACPE MOH
Kazantseva O.M.; Novokuznetsk State Institute for Further Training of Physicians, Branch Campus of the FSBEI FPE RMACPE MOH
Arkhipova S.V.; Novokuznetsk State Institute for Further Training of Physicians, Branch Campus of the FSBEI FPE RMACPE MOH
Khanin A.L.; Novokuznetsk State Institute for Further Training of Physicians, Branch Campus of the FSBEI FPE RMACPE MOH
2020-06-23 17:28:03
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1366
коинфекция ВИЧ/туберкулез; предикция; исход заболевания; летальность; IgE; IgM; IgG; IgA; sIgA
ru
A search for prognostic markers of HIV and tuberculosis coinfection (HIV/TB), especially in case of Mycobacterium tuberculosis multidrug resistance (MDR MBT) associated with low rates of TB eradication, is of relevance in connection with the problem of choosing adequate anti-TB therapy which is able to decrease mortality. 113 HIV/TB patients aged 24 to 58 years were examined: 70 males and 43 females hospitalized at the Novokuznetsk TB Clinic during the 2017—2019 period. MDR MBT (concomitant resistance to Isoniazid and Rifampicin) was found in 50 patients (12 patients with MDR MBT had additional resistance to Fluoroquinolones) aged 24 to 54 years — 31 males and 19 females. The control group consisted of 49 healthy individuals aged 27 to 72 years (26 females and 23 males) lacking focal and systemic infections with moderately pronounced age-related changes. In plasma samples, concentration of total (non-specific) immunoglobulins of classes E, M, G, A (including secretory immunoglobulin A, sIgA) were measured by using enzyme-linked immunosorbent assay. Data statistical processing was performed by using licensed software packages InStatII, Microsoft Excel, IBM SPSS Statistics 22. An extended range of individual variability in count of peripheral blood CD4 lymphocytes was revealed both among non-survivor and survivor patients with HIV/TB examined, being a drawback of using such parameter as lethality predictor. It was found that the serum level of total IgE, IgM, IgG, IgA and sIgA in patients with HIV/ TB was higher than that one in control group, whereas in non-survivor vs. survivor patients the concentration of IgE and sIgA was elevated. The coefficient of disease outcome prediction (CP) for patients with HIV/TB and MDR MBT was calculated being equal to the ratio of the multiplication of serum concentration of IgE, IgM, IgA and secretory IgA to CD4 lymphocyte count (CP = IgE x IgM x IgA x sIgA/CD4). CP higher than 200 was detected in 77% non-survivor and 6% of survivor patients. The relative risk of death with CP > 200 was very high (OR = 56.7, p < 0.0001) being 8.5 times higher than that one upon CD4 < 200 (OR = 6.7, p = 0.0237). A positive correlation between CP and lethal outcome was more valuable than that of CD4. The data presented allow us to propose CP for clinical use as an effective prognostic criterion for HIV/TB with MDR MBT.
oai:iimmun.ru:article/1439
2022-04-12T15:58:13Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/1439
2022-04-12T15:58:13Z
Russian Journal of Infection and Immunity
Vol 11, No 4 (2021); 723-736
Etiology of influenza-like illnesses in the population of Novosibirsk city in the 2018–2019 epidemic season
https://iimmun.ru/iimm/article/download/1439/8239
https://iimmun.ru/iimm/article/download/1439/8240
https://iimmun.ru/iimm/article/download/1439/8241
https://iimmun.ru/iimm/article/download/1439/8242
https://iimmun.ru/iimm/article/download/1439/8243
https://iimmun.ru/iimm/article/download/1439/8244
https://iimmun.ru/iimm/article/download/1439/8245
https://iimmun.ru/iimm/article/download/1439/8246
https://iimmun.ru/iimm/article/download/1439/8247
https://iimmun.ru/iimm/article/download/1439/8248
https://iimmun.ru/iimm/article/download/1439/8249
https://iimmun.ru/iimm/article/download/1439/8250
https://iimmun.ru/iimm/article/download/1439/10889
Kurskaya O.G.; Federal Research Center of Fundamental and Translational Medicine
Anoshina A.V.; Novosibirsk Children’s Municipal Clinical Hospital No. 6
Leonova N.V.; Novosibirsk Children’s Municipal Clinical Hospital No. 6
Simkina O.A.; Novosibirsk Children’s Municipal Clinical Hospital No. 3
Komissarova T.V.; Novosibirsk Children’s Municipal Clinical Hospital No. 3
Esikova E.Y.; Novosibirsk Municipal Infectious Clinical Hospital No. 1
Pozdnyakova L.L.; Novosibirsk Municipal Infectious Clinical Hospital No. 1
Sobolev I.A.; Federal Research Center of Fundamental and Translational Medicine
Prokopyeva E.A.; Federal Research Center of Fundamental and Translational Medicine
Murashkina Т.A.; Federal Research Center of Fundamental and Translational Medicine
Kazachkova E.A.; Federal Research Center of Fundamental and Translational Medicine
Alekseev А.Y.; Federal Research Center of Fundamental and Translational Medicine
Danilenko D.M.; Smorodintsev Research Institute of Influenza
Komissarov A.B.; Smorodintsev Research Institute of Influenza
Stolyarov K.A.; Smorodintsev Research Institute of Influenza
Fadeev A.V.; Smorodintsev Research Institute of Influenza
Sominina A.A.; Smorodintsev Research Institute of Influenza
Shestopalov А.M.; Federal Research Center of Fundamental and Translational Medicine
Sharshov K.A.; Federal Research Center of Fundamental and Translational Medicine
2021-05-13 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1439
эпидемиология; острые респираторные вирусные инфекции; грипп; гриппоподобные заболевания;
этиология ОРВИ; Глобальная сеть по госпитальному надзору за гриппом
Сбор материала, выявление генетического материала респираторных вирусов, определение нуклеотидных последовательностей вируса гриппа выполнены в рамках исследования GISHN. Работы по выделению вируса гриппа в культуре клеток и антигенный анализ выполнены при поддержке гранта РНФ № 19-74- 10055.
ru
Influenza and other acute respiratory viral infections lead to a substantial incidence of severe cases and hospitalizations and so remain a global health problem. Within the frame of the Global Influenza Hospital Surveillance Network (GIHSN), we assessed the contribution of influenza and other respiratory viruses to severe cases of influenzalike diseases in patients hospitalized to the Novosibirsk infectious hospitals in the years 2018–2019. We analyzed 484 nasopharyngeal swabs collected from patients admitted to the hospitals with acute respiratory infections (ARI) using real-time polymerase chain reaction commercial kits. We confirmed viral etiology of ARI in 69.8% cases. Influenza viruses were detected in 47.1% cases, wherein concomitant circulation of influenza A(H1N1)pdm09 and A(H3N2) viruses was observed in 20.7% and 26% of patients, respectively, whereas influenza B virus was detected only in one sample. All analyzed influenza A viruses were antigenically similar to vaccine strains. Genetically, the Novosibirsk strains were closely related to influenza A viruses distributed in Russia and worldwide. Influenza A(H1N1)pdm09 virus was detected in all patients aged 0 to 14 years and required intensive care. Other respiratory viruses were detected in 36.4% of children and 5.8% of adults, and 8.3% of children had viral coinfection, whereas no cases of coinfection were detected in adults. The most common viruses in children were metapneumovirus — 12.8%, rhinovirus — 9.3% and respiratory syncytial virus — 8.0%. In adults, metapneumovirus, adenovirus, parainfluenza virus and rhinovirus were detected with a detection rate no exceeding 2%. In this study, we found no differences in the detection rate of the influenza virus due to concomitant chronic diseases, pregnancy, or smoking habits. At the same time, the detection rate of other respiratory viruses in non-smokers vs. smokers was significantly lower than in smokers and former smokers (26.15%, 66.67% and 62.50%, respectively). In addition, the level of detection of respiratory viruses in children with vs. without chronic pathology was significantly higher (55.3% and 38.7%, respectively). Thus, our and similar studies are important for monitoring and control of the infection.
oai:iimmun.ru:article/802
2022-04-12T15:47:52Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/802
2022-04-12T15:47:52Z
Russian Journal of Infection and Immunity
Vol 9, No 3-4 (2019); 559-567
Comprehensive assessment of specific antibodies on infectious activity of tick-borne encephalitis virus
https://iimmun.ru/iimm/article/download/802/4383
https://iimmun.ru/iimm/article/download/802/4384
https://iimmun.ru/iimm/article/download/802/4385
https://iimmun.ru/iimm/article/download/802/4386
https://iimmun.ru/iimm/article/download/802/4387
https://iimmun.ru/iimm/article/download/802/4388
https://iimmun.ru/iimm/article/download/802/4389
https://iimmun.ru/iimm/article/download/802/4390
https://iimmun.ru/iimm/article/download/802/4391
https://iimmun.ru/iimm/article/download/802/4392
https://iimmun.ru/iimm/article/download/802/4393
https://iimmun.ru/iimm/article/download/802/4394
Leonova G.N.; Somov Institute of Epidemiology and Microbiology
Majstrovskaya O.S.; Somov Institute of Epidemiology and Microbiology
Lubova V.A.; Somov Institute of Epidemiology and Microbiology
Sanina N.B.; Far Eastern Federal University
2019-11-15 13:26:19
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/802
вирус клещевого энцефалита; защитный титр антител; динамика нейтрализации вируса in vitro; ex vivo; in vivo
Работа выполнена в рамках научных проектов: (0545-2014-0011) Федерального агентства научных организаций, РНФ (15-15-00035-P).
ru
Vaccines for prophylactic immunization provide the most reliable and effective protection against the vast majority of infectious diseases. Tick-borne encephalitis (TBE) represents a high-priority medical issue at the territory of the Eurasian continent. Of great importance is assessing a role of distinct antibody titers especially low titers, observed quite often in vaccinated individuals, sometimes posing obstacles in determining a threshold of seropositivity as well as the level of specific protection against TBE virus. We aimed at obtaining data to assess antiviral activity of virus-specific antibodies with distinct titer levels based on the in vitro, ex vivo and in vivo experimental studies with a highly virulent Far-Eastern strain of tick-borne encephalitis virus. The in vitro, ex vivo and in vivo comprehensive experimental studies with a highly virulent Far-Eastern strain of tick-borne encephalitis virus (TBEV) were conducted and the dynamics of antiviral activity of virus-specific antibodies at variable titers (1:100–1:3200) was measured (timeframe ranged within 1–96 hours p.i.) to provide a rationale for evaluating the antiviral immune response. It was found that the in vitro experiments demonstrated that the IgG at 1:100 titer exerted a weak anti-TBEV neutralizing effect at all time-points examined. The IgG 1:400 titer caused a 2 log PFU/mL decline in TBEV Dal strain yield at 72 h post-infection, whereas at 1:3200 titer it completely suppressed TBEV replication throughout the observation period. The ex vivo experiments with blood serum obtained from vaccinated subjects demonstrating a range of TBEV antibody titers (sera from vaccinated individuals with varying anti-TBEV antibody titers) and in vivo (outinbred white mice) experiments revealed a delayed virus elimination for antibody titers at 1:100 and 1:200 as well as rapid virus elimination (1–2 days p.i.) for antibody titers greater than 1:400. Thus, antibody titer at 1:400 may be considered as the universal anti-TBEV protection threshold. In order to properly conclude regarding the revaccination schedule it is advised to start with testing blood serum for durability of anti-TBEV immune response. Subjects with TBEV antibody titers at 1:100 and 1:200 should be strongly recommended to undergo a mandatory revaccination. Such an approach is believed to be the most effective way toward enhancing efficacy of vaccine-mediated protection against TBE.
oai:iimmun.ru:article/1303
2023-08-06T17:11:27Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/1303
2023-08-06T17:11:27Z
Russian Journal of Infection and Immunity
Vol 10, No 1 (2020); 90-98
Measures counteracting 2016 spread of vaccine-derived poliomyelitis virus type 2 in Russian Federation
https://iimmun.ru/iimm/article/download/1303/7021
https://iimmun.ru/iimm/article/download/1303/7022
https://iimmun.ru/iimm/article/download/1303/7023
https://iimmun.ru/iimm/article/download/1303/7024
https://iimmun.ru/iimm/article/download/1303/7025
Popova A.Y.; Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing (Rospotrebnadzor)
Ezhlova E.B.; Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing (Rospotrebnadzor)
Melnikova A.A.; Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing (Rospotrebnadzor)
Morozova N.S.; Federal Budget Institution of Health ofRospotrebnadzor“Federal Centre ofHygiene and Epidemiology”
Mikhailova Y.M.; Federal Budget Institution of Health ofRospotrebnadzor“Federal Centre ofHygiene and Epidemiology”
Ivanova O.E.; FSBSI Chumakov Federal Scientific Center for Research and Development ofImmune-and- Biological Products of Russian Academy ofSciences; Institute of Translational Medicine and Biotechnology, First Moscow State Medical University, Ministry of Health of the Russian Federation (Sechenov University)
Kozlovskaya L.I.; FSBSI Chumakov Federal Scientific Center for Research and Development ofImmune-and- Biological Products of Russian Academy ofSciences; Institute of Translational Medicine and Biotechnology, First Moscow State Medical University, Ministry of Health of the Russian Federation (Sechenov University)
Eremeeva T.P.; FSBSI Chumakov Federal Scientific Center for Research and Development ofImmune-and- Biological Products of Russian Academy ofSciences
Gmyl A.P.; FSBSI Chumakov Federal Scientific Center for Research and Development ofImmune-and- Biological Products of Russian Academy ofSciences; Institute of Translational Medicine and Biotechnology, First Moscow State Medical University, Ministry of Health of the Russian Federation (Sechenov University)
Korotkova E.A.; A.N. Belozersky Institute ofPhysical-ChemicalBiology, Lomonosov Moscow State University
Baykova O.Y.; FSBSI Chumakov Federal Scientific Center for Research and Development ofImmune-and- Biological Products of Russian Academy ofSciences
Krasota A.Y.; FSBSI Chumakov Federal Scientific Center for Research and Development ofImmune-and- Biological Products of Russian Academy ofSciences; A.N. Belozersky Institute ofPhysical-ChemicalBiology, Lomonosov Moscow State University
Ivanenko А.V.; Federal Budget Institution ofHealth of Rospotrebnadzor “Centerfor Hygiene and Epidemiology in Moscow”
Yarmolskaya M.S.; Federal Budget Institution ofHealth of Rospotrebnadzor“Centerfor Hygiene and Epidemiology in Moscow”
Kovalchuk I.V.; Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing in the Stavropol region
Romanenko E.N.; Federal Budget Institution ofHealth of Rospotrebnadzor “Center for Hygiene and Epidemiology in the Stavropol Region”
2020-04-05 22:21:46
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1303
полиомиелит; полиовирус типа 2; полиовирус вакцинного происхождения; иммунизация; оценка риска; ответные меры
ru
Since April 2016 after global cessation of using trivalent oral poliovirus vaccine (tOPV) and switch to bivalent OPV consisting of polioviruses types 1 and 3 (the “switch”), any isolation of type 2 poliovirus has been regarded as an event of extreme importance requiring investigation, risk assessment and decision making. In 2016, 2 cases of isolated vaccine-derived poliovirus type 2 from healthy children was registered in Russia. Our study was aimed at on the assessing a risk of further spread of vaccine-derived poliovirus type 2 and provide measures for preventing its further spread based on epidemiological investigation and genetic characteristics of the isolated viruses. The cases were revealed within the surveillance program for poliomyelitis and acute flaccid paralysis syndrome conducted in the Russian Federation. The laboratory investigation was carried out in accordance with the algorithm adopted in the Russian Federation and recommended by the WHO standards: virus isolation on RD, L20B and Hep2C cell cultures, identification in the neutralization reaction, intratyping differentiation by using RT-PCR in real-time mode, sequencing of the poliovirus genome fragments encoding the VP1 protein. A risk assessment for spread of vaccine-derived poliovirus type 2 was performed in accordance with the WHO recommendations. There was uncovered a genetic relationship between virus strains isolated in September and December from unvaccinated Moscow resident boy (1 year old) who arrived from the Chechen Republic and from unvaccinated girl resident of the Chechen Republic (1 year old) with impaired humoral and cellular immunity. The virus strains were found to bear 10 and 13 genomic nucleotide substitutions, respectively, at the site encoding the VP1 protein compared with the Sabin type 2 vaccine strain that allowed to classify them as vaccine-derived polioviruses. In particular, both virus strains were shown to originate from the type 2 strain presented in the tOPV used shortly before the “switch”. Epidemiological investigation revealed family ties and probable contact between both children in the same premises. A series of organizational and vaccination measures was undertaken, as well as polio surveillance was strengthened in the region. No new type 2 polioviruses of vaccine origin were detected in the territory of the Chechen Republic during 18-month monitoring follow-up. The risk assessment of spread for vaccine-derived poliovirus type 2 in a region, Russian Federation as well as cross-boundary spread identified it as “low,” requiring no use of type 2 monovalent OPV. Such experience for countermeasures may be taken into account to oppose the risks before and after the global certification for poliomyelitis eradication.
oai:iimmun.ru:article/12421
2023-12-26T13:00:11Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/12421
2023-12-26T13:00:11Z
Russian Journal of Infection and Immunity
Vol 13, No 4 (2023); 735-742
Phylogenetic analysis of the Klebsiella pneumoniae uge gene in local microbiological monitoring
https://iimmun.ru/iimm/article/download/12421/109711
https://iimmun.ru/iimm/article/download/12421/109712
https://iimmun.ru/iimm/article/download/12421/109713
https://iimmun.ru/iimm/article/download/12421/109714
https://iimmun.ru/iimm/article/download/12421/109715
https://iimmun.ru/iimm/article/download/12421/109716
https://iimmun.ru/iimm/article/download/12421/109717
https://iimmun.ru/iimm/article/download/12421/109718
https://iimmun.ru/iimm/article/download/12421/121071
https://iimmun.ru/iimm/article/download/12421/121072
https://iimmun.ru/iimm/article/download/12421/121073
https://iimmun.ru/iimm/article/download/12421/121074
https://iimmun.ru/iimm/article/download/12421/139612
https://iimmun.ru/iimm/article/download/12421/139613
https://iimmun.ru/iimm/article/download/12421/153574
Ustyuzhanin A.V.; Ural Scientific Research Institute of Maternity and Child Care
Chistyakova G.N.; Ural Scientific Research Institute of Maternity and Child Care
Remizova I.I.; Ural Scientific Research Institute of Maternity and Child Care
Makhanyok A.A.; Ural Scientific Research Institute of Maternity and Child Care
2023-10-24 14:50:49
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/12421
секвенирование; филогенетический анализ; ген uge; Klebsiella pneumoniae; мониторинг; неонатология; эпидемиология
Array
ru
The aim of the study was to evaluate the data of phylogenetically analyzed nucleotide sequences from the K. pneumoniae strain uge genes carried out in the perinatal center. Materials and methods. Fifty-six sequences of the K. pneumoniae uge gene were analyzed. The uge gene was detected by real-time PCR using DT light amplifier (Russia). Results. The rate of K. pneumoniae strains among patients of obstetric and gynecological departments in 2020–2023 averaged 1.4%. In pediatric hospitals, the isolation of K. pneumoniae strains comprised 12–14% in 2020, 2021 and 2023. In 2022, a fourfold decrease in detected K. pneumoniae strains was recorded. Phylogenetic analysis showed that the nucleotide sequences were significantly grouped into 14 clusters. The K. pneumoniae virulence factor uge gene is found in 64.3% cases. The nucleotide sequences allowed to detect heterogeneous K. pneumoniae strain population isolated from patients at the perinatal center from 2019 to 2023 analyzed by the phylogenetic method. There are clusters that combine K. pneumoniae genovariants isolated in 2019 and not replenished with new isolates, which confirms the effectiveness of ongoing anti-epidemic measures, enhanced during COVID-19 spread, excluding the transmission of an infectious agent from a source to a susceptible organism in nosocomial environment. P249Q, N279L amino acid substitutions within the uge gene were determined, which distinguish hypervirulent strains from those with a lower degree of pathogenicity. Out of five mother-child pairs, in four — nucleotide sequences of K. pneumoniae strain uge gene were genetically closer to each other than to other strains isolated from patients of the Medical Departments suggesting about a high degree of their relationship, highly likely indicating that the source of the strain for the child was the paired mother, and not the patients of the departments or the staff of the institution. In one mother-child pair, K. pneumoniae strains belonged to different clusters. The isolate obtained on 09/06/2021 from neonatal faeces (age: 7 days old) was grouped with strains isolated on 12/12/2020 from a patient in the maternity ward and in Laos in 2013 (CP035196). K. pneumoniae isolated on 06/04/2021 from the urine of a woman, was significantly grouped with strains included in cluster 13. Conclusion. An opportunity for improving local microbiological monitoring by sequencing and phylogenetic analysis of K. pneumoniae uge gene has been demonstrated. Assessment of changes in the intraspecies population pattern of HCAI pathogens is necessary for a timely and reasonable response to the deterioration of the epidemiological situation.
oai:iimmun.ru:article/516
2022-04-12T15:20:08Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/516
2022-04-12T15:20:08Z
Russian Journal of Infection and Immunity
Vol 7, No 2 (2017); 162-170
EFFECTIVENESS OF MOLECULAR-GENETIC DIAGNOSTICS DURING PERTUSSIS INFECTION FOCI EXAMINATION
Pimenova A.S.; G.N. Gabrichevsky Institute of Epidemiology and Microbiology
Borisova O.Y.; G.N. Gabrichevsky Institute of Epidemiology and Microbiology;
The Russian National Research Medical University named after N.I. Pirogov
Tsvircun O.V.; G.N. Gabrichevsky Institute of Epidemiology and Microbiology
Basov A.S.; G.N. Gabrichevsky Institute of Epidemiology and Microbiology
Aleshkin V.A.; G.N. Gabrichevsky Institute of Epidemiology and Microbiology
Afanasiev S.S.; G.N. Gabrichevsky Institute of Epidemiology and Microbiology
Donskich E.E.; The Russian National Research Medical University named after N.I. Pirogov
Pikina A.P.; The Russian National Research Medical University named after N.I. Pirogov
Kafarskaya L.I.; The Russian National Research Medical University named after N.I. Pirogov
Afanasiev M.S.; Sechenov Moscow State Medical University
Karaulov A.V.; Sechenov Moscow State Medical University
2017-06-19 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/516
коклюш;молекулярно-генетическая диагностика;полимеразная цепная реакция (ПЦР);ПЦР в режиме реального времени;изотермальная амплификация;Bordetella pertussis
ru
Purpose: whooping cough diagnostics by molecular-genetic methods.Materials and methods. Under observation there were 4930 people during the period from 2012 to 2015. All samples were received in 8 schools of Moscow and the Moscow region: in 3 schools had been found children with whooping cough, in other 5 schools – only children with prolonged cough. Whooping cough diagnosis had been given earlier by bacteriological and serological methods. 430 clinical samples were studied by 2 methods: PCR with fluorescent hybridized detection of amplified products and isothermal amplification.Results. In three of eight schools whooping cough cases at 7 children at the age of 7, 9, 11 and 15 years were revealed earlier. The diagnosis of whooping cough at them was confirmed by means of bacteriological and serological methods. 33 positive DNA samples (7.7%) are revealed. DNA-positive samples are allocated from 18 pupils and 15 employees of schools. In two schools where earlier infection sources were established, 15 DNA-positive samples from which in three cases clinical manifestations were observed are revealed. In those schools where it wasn’t earlier established a source of an infection and examinations conducted it is long the coughing children, 18 DNA-positive samples are revealed, and in two cases clinical manifestations in the form of cough were observed.Conclusion. Performed research confirmed high efectiveness of molecular-genetic methods during pertussis infection foci examination in schools for infection source identification also amongst long coughing children.
oai:oai.iimmun.ru:article/1566
2020-08-07T07:04:11Z
iimm:ORIG
oai:iimmun.ru:article/781
2023-08-06T17:26:31Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/781
2023-08-06T17:26:31Z
Russian Journal of Infection and Immunity
Vol 8, No 3 (2018); 316-324
IDENTIFICATION MARKERS OF INFECTION DUE TO C. TRACHOMATIS AND C. PNEUMONIAE, IN PATIENTS WITH DISEASES OF THE GASTROINTESTINAL TRACT
Bondareva N.E.; Gamaleya Research Center of Epidemiology and Microbiology.
Morgunova E.Y.; Gamaleya Research Center of Epidemiology and Microbiology.
Zigangirova N.A.; Gamaleya Research Center of Epidemiology and Microbiology.
Shapkin Y.G.; Saratov State Medical University named after V.I. Razumovsky.
Chalyk Y.V.; Saratov State Medical University named after V.I. Razumovsky.
Chalyk R.Y.; Saratov City Clinical Hospital No. 6 named after academician V.N. Kosheleva.
2018-11-01 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/781
trachomatis; C. pneumoniae; хламидийная инфекция; персистентная инфекция; желудочно-кишечный тракт; ПЦР
ru
To date, clinical data have convincingly shown that C. trachomatis and C. pneumoniae infectious can cause serious diseases with severe complications and consequences. There are assumptions that the developed chronic chlamydial infection can become an important factor in the pathogenesis of the gastrointestinal tract diseases, which are manifested in the so-called post-infectious period. It is commonly known that chlamydial infection has a tropism to the cylindrical epithelium, which covers the human mucous membrane of the urethra, cervix, rectum, conjunctiva of the eyes and the throat. However, the role of the causative agents of chlamydial infections, such as C. trachomatis and C. pneumoniae, in the occurrence of the gastrointestinal tract diseases has not been studied. In order to study the possible relationship between the gastrointestinal diseases and the presence of chlamydial infection markers, we have selected a group of patients with the gastrointestinal diseases and detected antibodies to C. trachomatis and C. pneumoniae and DNA of these pathogens in blood serum, liver biopsy and bile ducts. As a result, C. trachomatis DNA in blood serum was detected in 50% of cases, and in liver biopsies — in 59.3%. A new approach has been developed in the serological diagnosis of chlamydial infection caused by C. trachomatis, which allowed for revealing diagnostic antibody titers in 51.9% of cases in this group of patients, and in the comparison group — in 11.6% of cases. Among 50% of patients, in whom DNA was revealed in blood serum, it was also revealed in 64.3% of cases in biopsy samples of gastrointestinal organs. Upon detection of C. trachomatis DNA in blood serum, antibodies to the “cultural” antigen were detected in 60.1% of cases, and with the simultaneous detection of C. trachomatis DNA in blood serum and gastrointestinal organs, they were found in 72.2% of cases. Simultaneous detection of C. trachomatis, both in blood serum and in the gastrointestinal tract, may indicate the ability of C. trachomatis to spread hematogenously and infect organs away from the primary focus of infection. The obtained data absolutely require further study in light of the identification of the relationship between the detection of the pathogen and the development of the gastrointestinal pathology. But in general, the results are not yet studied evidence of the possible gastrointestinal organs infection by C. trachomatis.
oai:iimmun.ru:article/110
2022-04-12T21:18:55Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/110
2022-04-12T21:18:55Z
Russian Journal of Infection and Immunity
Vol 2, No 4 (2012); 729-734
MODERN APPROACHES IN COMPLEX LABORATORY TESTING FOR DIPHTHERIA
Kraeva L.A.; ФБУН НИИ эпидемиологии и микробиологии имени Пастера, Санкт-Петербург
Alekseeva E.A.; ФГУЗ Центр гигиены и эпидемиологии в Вологодской области, г. Вологда
Tseneva G.Y.; ФБУН НИИ эпидемиологии и микробиологии имени Пастера, Санкт-Петербург
Lipatova L.A.; ФГУЗ Центр гигиены и эпидемиологии в Ленинградской области, Санкт-Петербург
Bespalova G.I.; ГБОУ ВПО Северо-Западный государственный медицинский университет им. И.И. Мечникова Минздравсоцразвития России, Санкт-Петербург
2014-07-04 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/110
дифтерия; поствакцинальный иммунитет; токсигенные штаммы
ru
Abstract. The criteria of reliable validation of population protection against diphtheria on the base of two indices including quantity of antitoxic antibodies to diphtheria and avidity index have been established. For this purposes it was proposed to use the modified variant of ELISA allowed to detect both indices simultaneously. The formula of probable development of diphtheria in case of the close contact with patients or Corynebacterium diphtheriae bacteria carriers as well as determination of revaccination time have been proposed. The authors developed “The algorithm of population immunity control and assessment of non-susceptibility to diphtheria” and “The algorithm of micro biological testing of clinical samples for C. diphtheriae”.
oai:iimmun.ru:article/1864
2023-11-19T05:26:25Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/1864
2023-11-19T05:26:25Z
Russian Journal of Infection and Immunity
Vol 12, No 6 (2022); 1136-1142
A correlation between LP-PLA2 and monocyte levels in atherosclerosis risk subjects
https://iimmun.ru/iimm/article/download/1864/12423
https://iimmun.ru/iimm/article/download/1864/12425
https://iimmun.ru/iimm/article/download/1864/14830
Kumboyono K.; University of Brawijaya
Chomsy I.N.; University of Brawijaya
Vijayadas D.; University of Brawijaya
Wihastuti T.A.; University of Brawijaya
2022-12-30 21:01:07
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1864
атеросклероз; фермент; Lp-PLA2; клетки воспаления; популяция моноцитов; проатерогенный маркер
Array
en
Background. Cardiovascular disease (CVD) is the most significant cause of death worldwide. More than 17.9 million people died from CVD, of which 85% deceased due to heart disease. On the other hand, atherosclerosis is one of the most dominant CVD in most developing countries and developed countries. Lp-PLA2 is an enzyme produced by inflammatory cells and a pro-atherogenic protein in atherosclerosis. In the process, monocytes will produce Lp-PLA2 so that it may hydrolyse oxidized low density lipoprotein (oxLDL) into lysophosphatidylcholine (lysoPC) and oxidized fatty acids (oxFA), atherogenic proteins involved in atherogenesis. A chronic inflammatory process that occurs in atherosclerosis requires early detection to avoid CVD severity. The research aims to determine the correlation between Lp-PLA2 concentration and monocyte count as well as percentage in cohorts linked to risk of atherosclerosis. Materials and methods. This study was a descriptive correlational analysis of the population with conditions at risk of atherosclerosis. The total number of respondents sampled in this research was 86. We used the ELISA method to measure Lp-PLA2 concentration and the Hematology Analyzer method to measure monocyte count and percentage. Results. The relationship between monocyte and Lp-PLA2 level accounts for a probability value of 0.028. The correlation coefficient of 0.789 is categorized as very strong. Conclusion. Increase in the concentration of Lp-PLA2 correlates with monocyte count and percentage in a population with conditions at risk of atherosclerosis.
oai:iimmun.ru:article/1406
2022-04-12T15:54:59Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/1406
2022-04-12T15:54:59Z
Russian Journal of Infection and Immunity
Vol 11, No 1 (2021); 131-136
Pertussis immunity in pregnant women and factors associated with seronegative status
https://iimmun.ru/iimm/article/download/1406/7982
https://iimmun.ru/iimm/article/download/1406/7983
https://iimmun.ru/iimm/article/download/1406/7984
https://iimmun.ru/iimm/article/download/1406/7985
https://iimmun.ru/iimm/article/download/1406/7986
https://iimmun.ru/iimm/article/download/1406/7987
https://iimmun.ru/iimm/article/download/1406/7988
https://iimmun.ru/iimm/article/download/1406/8861
https://iimmun.ru/iimm/article/download/1406/8862
https://iimmun.ru/iimm/article/download/1406/9946
Krieger E.A.; Northern State Medical University
Samodova O.V.; Northern State Medical University
Titova L.V.; Northern State Medical University
2021-02-27 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1406
иммунитет; коклюш; беременные; вакцинация; антитела; серопревалентность
ru
Despite high level of vaccination coverage, pertussis remains a serious problem of modern medicine. Pertussis cases are registered among infants, adolescents, and adults. Infants younger than three months of age have the highest rate of serious clinical pertussis course. Transplacental transfer of pertussis-specific antibodies induce protection against infection. The available data regarding anti-pertussis antibody level in pregnant women in Russia remain sparce. To evaluate the humoral immunity to Bordetella pertussis in pregnant women and factors associated with seronegative status, we performed a cross-sectional study with 388 participants. SeroPertussis IgG (Israel) ELISA kit was used to quantitate antibodies against pertussis toxin/hemagglutinin. Binary logistic regression analysis was performed to assess factors associated with seronegative status. The median age of the subjects was 30 years old, more than half of which (51.3%) provided no verified pertussis vaccination data so that their children will not receive transplacental anti-infectious immunity. Gestational age was significantly associated with seronegative status. Compared to women tested during the first trimester, participants in the third trimester of pregnancy were more likely to be seronegative against pertussis. The odds of being susceptible rose with increased gestational period (p < 0.01 for linear trend). Age, number of pregnancies and vaccination status revealed no impact on significant differences between seropositive and seronegative subjects. Pertussis booster vaccinations for preschool children, adolescents and healthcare workers dealing with pregnant women and neonates as well as cocoon vaccination strategy and vaccination during pregnancy were necessary to be implemented to protect infants against pertussis.
oai:iimmun.ru:article/641
2023-08-06T17:12:18Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/641
2023-08-06T17:12:18Z
Russian Journal of Infection and Immunity
Vol 9, No 1 (2019); 76-86
Induction of HCV-specific cell response in vitro by dendritic cells generated with interferon-α
https://iimmun.ru/iimm/article/download/641/3279
https://iimmun.ru/iimm/article/download/641/3280
https://iimmun.ru/iimm/article/download/641/3281
https://iimmun.ru/iimm/article/download/641/3282
https://iimmun.ru/iimm/article/download/641/3283
https://iimmun.ru/iimm/article/download/641/3284
https://iimmun.ru/iimm/article/download/641/3285
https://iimmun.ru/iimm/article/download/641/3286
https://iimmun.ru/iimm/article/download/641/3287
https://iimmun.ru/iimm/article/download/641/3288
https://iimmun.ru/iimm/article/download/641/3289
https://iimmun.ru/iimm/article/download/641/3290
https://iimmun.ru/iimm/article/download/641/5612
https://iimmun.ru/iimm/article/download/641/5613
Chernykh E.R.; Research Institute of Fundamental and Clinical Immunology
Oleynik E.A.; Research Institute of Fundamental and Clinical Immunology
Leplina O.Y.; Research Institute of Fundamental and Clinical Immunology
Tikhonova M.A.; Research Institute of Fundamental and Clinical Immunology
Tyrinova T.V.; Research Institute of Fundamental and Clinical Immunology
Starostina N.M.; Research Institute of Fundamental and Clinical Immunology
Ostanin A.A.; Research Institute of Fundamental and Clinical Immunology
2019-03-30 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/641
HCV-инфекция; Т-клеточный ответ; Core-антиген; NS3-антиген; дендритные клетки
Array
ru
The induction of a strong multi-epitope T-cell response against hepatitis C virus (HCV) plays an important role in eliminating the virus, whereas adoptive response deficiency contributes to chronic and rapid progression of HCV-infection. Since dendritic cells (DCs) are capable of priming naive T lymphocytes and induce an effective immune response, the use of DC-based vaccines to enhance the HCV-specific T cell response is considered as a new approach to treatment of chronic hepatitis C (CHC). The ability of DCs generated from monocytes in the presence of interferon-α and loaded with recombinant HCV proteins Core (1–120) and NS3 (1192–1457) to induce an antigen-specific cellular response in healthy donors and patients with CHC was investigated. The immune response was assessed by proliferative activity and Th1 (IFNγ)/Th2 (IL-4, IL-6) production in mononuclear cells (MNC) cultures, and activation of cytotoxic T-lymphocytes in the degranulation test. We demonstrated that the primary antigen-specific response in MNC cultures of seronegative donors was detected better by stimulation of DCs, loaded with both antigens (DCCore /NS3) than when loaded with a single protein. DCCore/NS3 induced the proliferative response and degranulation of CD8+ T cells in MNC cultures of all tested donors, and in 50% (5/10) cases — IFNγ production. Similarly to donor DCs, DCCore/NS3 of patients with CHC induced a proliferative response in most cases (86%) and IFNγ production in 57% cases. At the same time, the activation of cytotoxic T cells in patients was less frequent (patients vs donors 57 and 100%, respectively), which could be partly due to increased spontaneous degranulation of CD8+ T cells in some patients. The obtained data testify the possibility of using vaccines based on interferon-α-induced DCs for the prevention and treatment of chronic HCV infection.
oai:iimmun.ru:article/769
2023-08-06T17:11:52Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/769
2023-08-06T17:11:52Z
Russian Journal of Infection and Immunity
Vol 9, No 5-6 (2019); 648-654
Genetic and phenotypic characteristics of <i>Klebsiella michiganensis</i> isolates
https://iimmun.ru/iimm/article/download/769/4243
https://iimmun.ru/iimm/article/download/769/4244
https://iimmun.ru/iimm/article/download/769/4245
https://iimmun.ru/iimm/article/download/769/4246
https://iimmun.ru/iimm/article/download/769/4247
https://iimmun.ru/iimm/article/download/769/4248
https://iimmun.ru/iimm/article/download/769/4249
https://iimmun.ru/iimm/article/download/769/5981
https://iimmun.ru/iimm/article/download/769/5982
Sivolodskii E.P.; S.M. Kirov Military Medical Academy; St. Petersburg Pasteur Institute
Freylikhman O.A.; St. Petersburg Pasteur Institute
2020-01-30 09:31:32
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/769
Klebsiella michiganensis; Klebsiella kielensis; Klebsiella oxytoca; уникальный фермент клебсиелл 5-АСК декарбоксилаза; секвенирование гена rpoB
ru
The aim of the study was to identify an optimal research target for detection of Klebsiella michiganensis isolates, determine their genetic and phenotypic characteristics necessary for identification. Here, we examined 11 Klebsiella oxytoca strains, lacking (atypical, negative) a marker 5-aminosalicylate decarboxylase (detected by the chromogenic reaction by 5-aminosalicylic acid) unique for the genus Klebsiella bacteria. They were selected for genetic analysis subsequent to a phenotypic characterization of K. oxytoca clinical isolates, collected in within 2015–2018 period in medical institutions in St. Petersburg. Two K. oxytoca and two Raoultella ornithinolytica clinical strains displaying typical properties were used as a control. The presence of 5-aminosalicylate decarboxylase was detected by the chromogenic reaction with the “Klebsiella 5-ASK CHROME C” nutrient medium (Pasteur Institute, St. Petersburg). Substrate utilization as the sole carbon source was detected on a solid minimal synthetic medium added with 2 g/L substrate during incubation for 72 hours at 37°C. Biochemical bacteria features were studied by the microvolume method with the “Rapid-Entero” test system (Pasteur Institute, St. Petersburg). Genetic strain characterization was performed by estimating 16S rRNA, gyrA, rpoB by using a routine PCR with primer sequences described before. Two rpoB gene fragments with a total length 834 bp, 16S rRNA gene fragment — 387 bp, and gyrA gene fragment — 441 bp were amplified followed by their sequencing by Singer on an ABI 3130 automatic capillary sequencer (Applied Biosystems, USA) and subsequently determined similarity levels. Amplification pattern for pehX gene PCR fragments was performed by using a method described elsewhere with two primer pairs flanking fragment AD with a 513 bp length and 344 bp CD-long motifs. While examining 11 clinical bacterial strains identified earlier as Klebsiella oxytoca, lacking (atypical, negative) a 5-aminosalicylate decarboxylase (detected by the chromogenic reaction by 5-aminosalicylic acid) unique for the genus Klebsiella, molecular techniques identified 9 K. michiganensis strains and 1 strain highly homologous to Klebsiella kielensis based on the rpoB gene nucleotide sequence, confirming its high informative value. We used the methods for estimating a similarity level for sequenced fragments of 16S rRNA genes (fragment length 387 bp), gyrA gene (fragment length 441 bp), rpoB gene (rpoB-b and rpoB-e with a total fragments length 834 bp), and the analysis of marker amplicon patterns for pehX gene (AD, CD). It was shown that for the 4 K. oxytoca strains, 99–100% similarity to K. michiganensis was identified for all fragments in the sequenced genes. Moreover, similarity of all 9 strains detected with K. michiganensis was revealed only in the rpoB gene, hereby allowing to recommend it as the most informative approach. The pehX gene encoding polygalacturonase was verified by PCR in the majority of K. michiganensis strains, pointing that this approach is not rational for their identification (distinguish with K. oxytoca). The most informative for the phenotypic identification of K. michiganensis are were assays characterized by a common profile for the majority of strains: lack of 5-aminosalicylate decarboxylase, lack of utilized histamine, dulcitol, tricarballylic acid; positive for indole production, as well as D-melezitose and putrescine utilization.
oai:iimmun.ru:article/8034
2023-12-16T10:34:40Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/8034
2023-12-16T10:34:40Z
Russian Journal of Infection and Immunity
Vol 13, No 5 (2023); 931-938
Biochemical markers assessing rate of reparative processes in patients with infiltrative pulmonary tuberculosis
https://iimmun.ru/iimm/article/download/8034/73763
https://iimmun.ru/iimm/article/download/8034/73764
https://iimmun.ru/iimm/article/download/8034/73831
https://iimmun.ru/iimm/article/download/8034/73832
https://iimmun.ru/iimm/article/download/8034/73833
https://iimmun.ru/iimm/article/download/8034/73834
https://iimmun.ru/iimm/article/download/8034/73835
https://iimmun.ru/iimm/article/download/8034/153760
Esmedlyaeva D.S.; St. Petersburg State Research Institute of Phthisiopulmonology of the Ministry of Healthcare
Alekseeva N.P.; St. Petersburg State University
Dyakova M.E.; St. Petersburg State Research Institute of Phthisiopulmonology of the Ministry of Healthcare
2023-11-30 23:31:12
Authors who publish with this journal agree to the following terms:
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url:https://iimmun.ru/iimm/article/view/8034
туберкулез; репарация; биомаркеры; матриксные металлопротеиназы; тканевые ингибиторы матриксных металлопротеиназ; нейтрофильная эластаза
Array
ru
The effectiveness of chemotherapy for infiltrative pulmonary tuberculosis (ITL) is determined by proper timing and adequacy of treatment. The speed of reparative changes in the pulmonary parenchyma is associated with the effectiveness of therapy. Different classes of proteinases are involved in extracellular matrix remodeling. The aim of the study was to evaluate a potential of using the markers of proteinase/inhibitors axis for predicting effectiveness of therapy in patients with ITL due to varying resistance of Mycobacterium tuberculosis to anti-tuberculosis drugs (PTP). Materials and methods. A retrospective study included 60 and 55 ITL patients with the drug sensitive (DS) and drug resistance (DR) Mtb strains, respectively. Patients were divided into two groups according to therapy effectiveness. The levels of blood serum matrix metalloproteinase MMP-1, MMP-3, MMP-8, MMP-9 and their TIMP-1 inhibitor, neutrophil elastase (NE), alpha-2 macroplobulin (MG) and proteinase inhibitor (PI) were determined. Statistica 10.0 and R software packages were used. Results. According to the model of discriminant analysis, the pattern of host inflammatory response in ITL was accounted for not only by varying pathogen resistance, but also by the different size of lung damage. Patients with a limited and widespread process with the DS of the pathogen strains were assigned to group I and II, and DR — to group III and IV group, respectively. The decisive in the speed of tissue repair was the number of neutrophils bearing various levels of proteinase MMP-8, MMP-9 (I group) or TIMP-1 and PI inhibitors (II group). Only combining biochemical data with the those on radiation methods are possible for prognosis in a group of patients with DR (III and IV). Conclusions. Combinations of the markers of the proteinase/inhibitors axis are informative in assessing a rate of reparative changes in ITL and differ not only depending on the pathogen DS strains, but also from the size of destructed pulmonary parenchyma. Differences in parameter combinations are accounted for by history of a specific disease.
oai:iimmun.ru:article/593
2022-04-12T15:20:57Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/593
2022-04-12T15:20:57Z
Russian Journal of Infection and Immunity
Vol 7, No 4 (2017); 359-366
HPV-ASSOCIATED DISEASES IN THE STRUCTURE OF FEMALE REPRODUCTIVE TRACT TUMORS AND SEXUALLY TRANSMITTED INFECTIONS IN MOSCOW AND RUSSIA
Briko N.I.; Sechenov University, Moscow
Lopukhov P.D.; Sechenov University, Moscow
Kaprin A.D.; P. Herzen Moscow Oncology Research Institute, Moscow
Novikova E.G.; P. Herzen Moscow Oncology Research Institute, Moscow
Trushina O.I.; P. Herzen Moscow Oncology Research Institute, Moscow
Khaldin A.A.; Moscow Research and Practical Center of Dermatology, Venereology and Cosmetology (MRPCDVC), Moscow
Zhukova O.V.; Moscow Research and Practical Center of Dermatology, Venereology and Cosmetology (MRPCDVC), Moscow
Isaeva D.R.; Moscow Research and Practical Center of Dermatology, Venereology and Cosmetology (MRPCDVC), Moscow
Skvortsova A.I.; Moscow Research and Practical Center of Dermatology, Venereology and Cosmetology (MRPCDVC), Moscow
2018-01-20 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/593
вирус папилломы человека; рак шейки матки; аногенитальные бородавки; инфекции передаваемые половым путем; заболеваемость; смертность
ru
The group of female reproductive tract tumors includes cancers of corpus uteri, ovary, cervix, vulva and vagina (the last three are associated with HPV). Among the large group of sexually transmitted infections (STIs) there are only six infections, which subject to registration in the official statistical data in Russia: syphilis, gonorrhea, trichomoniasis, chlamydia, urogenital herpes and anogenital warts (AGWs). Occurrence of AGWs in 95–100% of cases caused by HPV types 6 and 11. Objectives: our aim was to determine the prevalence of HPV-associated diseases (cervical cancer and anogenital warts) in the structure of female reproductive tract tumors and STIs in recent years in Moscow, and to compare it with the prevalence in population of Russia. Methods: we analyzed retrospective data with diagnostic codes related to cancers of corpus uteri, ovary, cervix, vulva and vagina from the official statistic of cancer register using incidence and mortality rates between January 2007 and December 2015 in Russia and Moscow. We also analyzed retrospective data of STIs (incl. syphilis, gonorrhea, trichomoniasis, chlamydia, urogenital herpes and AGWs) incidence rates between January 2003 and December 2015. Results: average rate of cervical cancer incidence among the female reproductive tract tumors accounted for 23.7% in Moscow (29.8% in Russia). Average rate of cervical cancer mortality among the female reproductive tract tumors accounted for 24.1% in Moscow (28.3% in Russia). The rate of AGWs in the STIs structure (for both sexes) in Moscow increased from 11.5% in 2003 to 25.7% in 2015 (the maximum was 31.0% in 2014). The highest rate of AGWs was detected in the age group 15–17 years (up to 59.8% in 2012), followed by 18–29 years (up to 37.3% in 2014). The rate of AGWs was 11.7% among all STIs (in both sexes) in Russia in 2015. Conclusion: HPV-associated diseases (cervical cancer and AGWs) take up a significant place in the structure of female reproductive tract tumors and STIs. And in the structure of the STI for Moscow, AGWs moved from the 5th (in 2003) to the 1st (in 2015) ranked place, surpassing syphilis, gonorrhea, trichomoniasis and chlamydia. The effective prevention of HPV-infection would be able to improve the situation.
oai:iimmun.ru:article/38
2022-04-12T21:19:45Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/38
2022-04-12T21:19:45Z
Russian Journal of Infection and Immunity
Vol 1, No 2 (2011); 135-142
EPIDEMIOLOGICAL CHARACTERISTICS OF HEPATITIS A INFECTION IN THE PRE-VACCINATION AND VACCINATION PERIODS
Loshkareva V.N.; МУЗ «Детская городская клиническая больница № 3», г. Пермь
ГОУ ВПО «Пермская государственная медицинская академия имени академика Е.А. Вагнера» Росздрава, г. Пермь
Semerikov V.V.; Управление здравоохранения Администрации г. Перми
ГОУ ВПО «Пермская государственная медицинская академия имени академика Е.А. Вагнера» Росздрава, г. Пермь
2014-06-26 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/38
гепатит А; вакцинация; дети
ru
Abstract. The hepatitis A has important social and economic significance in the Russian Federation now. The analysis of multiyear dynamics of hepatitis A incidence in the regional center — Perm city has been carried out. It was established that vaccination of children against hepatitis A within the different institutions influenced on the intensity of epidemic process. The hepatitis A incidence rates significantly decreased in the vaccinated groups and in the whole population. The typical seasonal distribution of cases and their clustering became less manifested.
oai:iimmun.ru:article/646
2023-08-06T17:12:24Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/646
2023-08-06T17:12:24Z
Russian Journal of Infection and Immunity
Vol 9, No 1 (2019); 193-202
Russia-wide epidemiological survey of congenital and nosocomial infections in newborns
https://iimmun.ru/iimm/article/download/646/3440
https://iimmun.ru/iimm/article/download/646/3441
https://iimmun.ru/iimm/article/download/646/3442
https://iimmun.ru/iimm/article/download/646/3443
https://iimmun.ru/iimm/article/download/646/3444
https://iimmun.ru/iimm/article/download/646/3445
https://iimmun.ru/iimm/article/download/646/3446
https://iimmun.ru/iimm/article/download/646/3447
https://iimmun.ru/iimm/article/download/646/3448
https://iimmun.ru/iimm/article/download/646/3449
https://iimmun.ru/iimm/article/download/646/5526
Ivanova M.V.; I.M. Sechenov First Moscow State Medical University (Sechenov University)
Mindlina A.Y.; I.M. Sechenov First Moscow State Medical University (Sechenov University)
Polibin R.V.; I.M. Sechenov First Moscow State Medical University (Sechenov University)
Ushanova A.V.; I.M. Sechenov First Moscow State Medical University (Sechenov University)
2019-03-21 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/646
внутриутробные инфекции; внутрибольничные инфекции; инфекции; связанные с оказанием медицинской помощи; регистрация; заболеваемость; смертность
Array
ru
High prevalence and great socio-economic impact of nosocomial infections is documented in obstetric hospitals. Objective: to uncover Russia-wide epidemiological features for congenital and nosocomial infections in newborns. Material and Methods: the data covering 2008–2016 period were retrieved from the Federal Statistical Monitoring Form No. 2 “Report on Infectious and Parasitic Diseases”, via the Unified Interdepartmental Information Statistical System. During 2008–2016, incidence of congenital and nosocomial infections in newborns was analyzed in various constituents of the Russian Federation, which were divided into quartile groups including confidence interval. Results: It was found that the increased/decreased/unchanged incidence of congenital infections in newborns was shown in 30, 31 and 13 subjects, respectively. In case of nosocomial infections in newborns, its incidence was increased/decreased/unchanged in 7, 41 and 26 subjects, respectively. Moreover, a congenital/nosocomial infection ratio in newborns was increased/decreased/ unchanged in 40, 15 and 19 subjects, respectively. Based on the morbidity pattern, 9 major groups might be distinguished. In particular, the most abundant (16 subjects) was the group with increased incidence of congenital infections, decreased incidence of nosocomial infections and increased congenital/nosocomial infection ratio in newborns. However, it is worth mentioning that recording of nosocomial infection cases is incomplete in the vast majority of the subjects of the Russian Federation: as few as seven subjects may be highlighted providing a proper registration of nosocomial and congenital infections in newborns such as: Vologda Region, Trans-Baikal Territory, Nizhny Novgorod Region, Omsk Region, Orenburg Region, Penza Region, and Sverdlovsk Region. Conclusion: to fully assess an objective status on nosocomial and congenital infections in newborns, it is necessary that a hospital epidemiologist investigates every single case; ensure applying a standard approach to determine a case of healthcare-associated infection, congenital infections; official recording of all cases; responsibility of all staff members involved in recording healthcare-associated infection cases; regularly reviewed data by using science-based methods. Key words: congenital infections, nosocomial infections, healthcare associated infections, registration, incidence, mortality.
oai:iimmun.ru:article/1758
2023-11-19T05:06:22Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/1758
2023-11-19T05:06:22Z
Russian Journal of Infection and Immunity
Vol 12, No 5 (2022); 875-890
Analyzying the documented results by using microscopic agglutination test to examine sera from patients suspected of leptospirosis
https://iimmun.ru/iimm/article/download/1758/11208
https://iimmun.ru/iimm/article/download/1758/11209
https://iimmun.ru/iimm/article/download/1758/11210
https://iimmun.ru/iimm/article/download/1758/11211
https://iimmun.ru/iimm/article/download/1758/11212
https://iimmun.ru/iimm/article/download/1758/11213
https://iimmun.ru/iimm/article/download/1758/11214
https://iimmun.ru/iimm/article/download/1758/11215
https://iimmun.ru/iimm/article/download/1758/0
https://iimmun.ru/iimm/article/download/1758/0
https://iimmun.ru/iimm/article/download/1758/12377
https://iimmun.ru/iimm/article/download/1758/12378
https://iimmun.ru/iimm/article/download/1758/12379
https://iimmun.ru/iimm/article/download/1758/12753
Samsonova A.P.; N.F. Gamaleya Federal Research Center for Epidemiology and Microbiology; I.M. Sechenov First Moscow State Medical University (Sechenov University)
Petrov E.M.; N.F. Gamaleya Federal Research Center for Epidemiology and Microbiology
Savelyeva O.V.; N.F. Gamaleya Federal Research Center for Epidemiology and Microbiology
Ivanova A.E.; N.F. Gamaleya Federal Research Center for Epidemiology and Microbiology
Sharapova N.E.; N.F. Gamaleya Federal Research Center for Epidemiology and Microbiology
2022-11-16 17:27:27
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1758
лептоспиры; лептоспирозы; природные очаги лептоспирозов; хозяйственные очаги лептоспирозов; реакция микроагглютинации; серогруппа
Array
056-00119-21-01
ru
Leptospirosis holds one of the leading places among zoonoses due to the breadth of distribution of natural and economic foci, as well as the severity of the clinical manifestation and mortality rate. A relatively low incidence rate in some regions is usually accounted for by unsatisfactory level of differential diagnostics, including insufficient coverage by diagnostic tests. Leptospira are characterized by marked phenotypic and genotypic polymorphisms, affecting epidemiological features and clinical manifestation of leptospirosis. Therefore, laboratory methods are important tool for epidemiological surveillance and diagnostics of leptospirosis. Serological methods primarily the microscopic agglutination test (MAT) currently remain the leading tool in investigating leptospirosis. To study a near 60 year-long contribution of various leptospira serogroups to etiological pattern of leptospirosis, we have analyzed archived MAT serum data from patients suspected of leptospirosis retrieved from our laboratory journals during from the years 1962 to 2020. It was shown that antibodies to the Grippotyphosa serogroup (25.1%) were found most often in patients, which might be associated with the wide range of reservoir host animals and resistance of this leptospira serogroup to environmental factors. At the same time, it should be noted that at different periods antibodies to various leptospira serogroups prevailed in the sera of patients, and in many cases this data matched with the contribution of these pathogens to the etiological morbidity pattern. In particular, dominance of pathogens of the Sejroe serogroup is currently the “global trend” in the etiological pattern of leptospirosis that was also observed by us at some years. The data obtained might be useful for epidemiological surveillance of leptospirosis infection, including predicting potential outbreaks, as well as for developing approaches to its diagnostics, treatment and prevention.
oai:iimmun.ru:article/133
2022-04-12T21:20:45Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/133
2022-04-12T21:20:45Z
Russian Journal of Infection and Immunity
Vol 3, No 3 (2013); 229-234
THE CELLS WITH MYCOBACTERIA IN GRANULOMATOUS AGGREGATES FROM MICE WITH LATENT TUBERCULOUS INFECTION IN EX VIVO CULTURE
Ufimtseva E.G.; The Institute of Biochemistry of the Siberian Branch of the Russian Academy of Medical Sciences, Novosibirsk
2014-07-07 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/133
латентная туберкулезная инфекция; вакцина БЦЖ; BCG-микобактерии; гранулема; макрофаги; дендритные клетки; клетки Пирогова–Лангханса.
ru
Abstract. The aim of this study was to obtain ex vivo monolayer culture cells migrated from individual granulomas isolated from the spleens of the Balb/c line mice through 1–2 months after BCG vaccine infection. The second goal was to evaluate influence of different types of cells in the development of granulomatic inflammation and analysis of BCG bacteria content in these cells in the latent stage of tuberculosis. Granulomas were presented by macrophages in general. The number of granulomas was varied as in one mouse as between mice. Granulomas contained also dendritic cells (in average 10% from macrophages of granulomas) and lymphocytes. In some granulomas fibroblasts, neutrophils, eosiniphils, multinuclear cells of Pirogov–Langhans, megacariocytes and platelets were observed in all stages of infection. The number of these cells was also varied between granulomas. The acid staining BCG bacteria were only detected in macrophages, dendritic cells and Pirogov–Langhans cells of mice granulomas. Mice were different as by number of cells with BCG bacteria in granulomas as by number of granulomas with BCG-containing cells. The proposed model of granuloma cells of mice in ex vivo culture can be used to study interaction between host cells and mycobacteria to find new ways and methods of influence to intracellular pathogens in latent stage of tuberculosis.
oai:iimmun.ru:article/2024
2023-04-01T17:30:02Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/2024
2023-04-01T17:30:02Z
Russian Journal of Infection and Immunity
Vol 13, No 1 (2023); 119-126
Pharmaceutical activity of a synthetic heterocyclic (C<sub>15</sub>H<sub>12</sub>N<sub>5</sub>OCl) compound on <i>Entamoeba histolytica</i> and <i>Giardia lamblia</i>
https://iimmun.ru/iimm/article/download/2024/14262
https://iimmun.ru/iimm/article/download/2024/14270
https://iimmun.ru/iimm/article/download/2024/14280
https://iimmun.ru/iimm/article/download/2024/14281
https://iimmun.ru/iimm/article/download/2024/14282
https://iimmun.ru/iimm/article/download/2024/14284
https://iimmun.ru/iimm/article/download/2024/14285
https://iimmun.ru/iimm/article/download/2024/14286
https://iimmun.ru/iimm/article/download/2024/14287
https://iimmun.ru/iimm/article/download/2024/14288
https://iimmun.ru/iimm/article/download/2024/14289
https://iimmun.ru/iimm/article/download/2024/14290
https://iimmun.ru/iimm/article/download/2024/14291
https://iimmun.ru/iimm/article/download/2024/15210
https://iimmun.ru/iimm/article/download/2024/15226
https://iimmun.ru/iimm/article/download/2024/15371
https://iimmun.ru/iimm/article/download/2024/15372
https://iimmun.ru/iimm/article/download/2024/74112
https://iimmun.ru/iimm/article/download/2024/74113
https://iimmun.ru/iimm/article/download/2024/74114
Obaid H.M.; Northern Technical University, College of Health and Medical Techniques
Sale S.S.; Kirkuk University, College of Science
Boundenga L.; International Centre for Medical Research of Franceville; Durham University
2023-04-01 18:41:32
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/2024
фармацевтика; активность; синтез; гетероциклическое соединение; Entamoeba histolytica; Giardia lamblia
Array
en
Background. Intestinal parasites are among the most important infectious agents with an impact on human health. Indeed, in the lack of an available treatment option, these parasites could constitute a real health problem in the population. In the present study, we investigated for the first time the effect of a novel synthetic heterocyclic ((C15H12N5OCL)2-(benzo(d)(1,2,3)triazol-1-yl)-N-benzylideneacetohydrazine) compound on two intestinal parasites (Entamoeba histolytica and Giardia lamblia). Methods. The parasite isolates tested were collected from outpatients at the General Pediatric Hospital in Kirkuk, Iraq, between September 2019 and January 2020. Thus, we studied the in vivo and in vitro pharmaceutical activity of the ingredient on both parasites. The toxicological effects of the substance on some blood parameters and liver and kidney function tests were also studied. Results. After five days of treatment, the drug’s in vivo action on G. lamblia resulted in an inhibition rate of 88.2% at a dose of 1 mg/kg. On the other hand, we observed that the influence of this synthetic substance on cultured E. histolytica was very close to the metronidazole effect. The maximum result was at a concentration of 1 g/ml and was obtained after 72 hours of incubation with an inhibition rate of 89.4%. The substance did not affect the blood parameters or the studied liver and kidney functions. Conclusion. It can be concluded that this substance is highly effective against both E. histolytica and G. lamblia, and that it has no toxic effects on the studied parameters. Therefore, it could be a promising pharmacophore for intestinal protozoan parasites including E. histolytica and G. lamblia and an alternative or competitor to the current medications available.
oai:iimmun.ru:article/273
2022-04-12T15:14:11Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/273
2022-04-12T15:14:11Z
Russian Journal of Infection and Immunity
Vol 5, No 1 (2015); 45-54
ETIOLOGICAL STRUCTURE OF VIRAL RESPIRATORY DISEASES IN ADULT PATIENTS WITH BRONCHIAL ASTHMA EXACERBATION AND CHRONICAL BRONCHIOLITIS OBLITERANS
Krivitskaya V.Z.; 197376, Russian Federation, St. Petersburg, Professor Popov str., 15/17, Research Institute of Influenza.
Sominina A.A.; 197376, Russian Federation, St. Petersburg, Professor Popov str., 15/17, Research Institute of Influenza.
Petrova M.A.; Research Institute of Pulmonology, Pavlov First St. Petersburg State Medical University, St. Petersburg, Russian Federation
Amosova I.V.; 197376, Russian Federation, St. Petersburg, Professor Popov str., 15/17, Research Institute of Influenza.
Mayorovа V.G.; 197376, Russian Federation, St. Petersburg, Professor Popov str., 15/17, Research Institute of Influenza.
Pisareva M.M.; 197376, Russian Federation, St. Petersburg, Professor Popov str., 15/17, Research Institute of Influenza.
Buzitskaya J.V.; 197376, Russian Federation, St. Petersburg, Professor Popov str., 15/17, Research Institute of Influenza.
Grudinin M.P.; 197376, Russian Federation, St. Petersburg, Professor Popov str., 15/17, Research Institute of Influenza.
Golovanova A.K.; 197376, Russian Federation, St. Petersburg, Professor Popov str., 15/17, Research Institute of Influenza.
Petrova E.R.; 197376, Russian Federation, St. Petersburg, Professor Popov str., 15/17, Research Institute of Influenza.
Kovaleva L.F.; Research Institute of Pulmonology, Pavlov First St. Petersburg State Medical University, St. Petersburg, Russian Federation
Kiseleva E.A.; Research Institute of Pulmonology, Pavlov First St. Petersburg State Medical University, St. Petersburg, Russian Federation
2015-04-21 00:00:00
Authors who publish with this journal agree to the following terms:
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Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/273
респираторные инфекции;вирусы;взрослые пациенты;бронхиальная астма;хронический бронхиолит;облитерация;вирусспецифические антитела
ru
Exacerbation of bronchial asthma (BA) and common deterioration of health during chronic bronchiolitis obliterans (ChBO) are associated with viral infections in adults in 64 and 83% respectively. Mixed virus-viral associations were shown in 21–25% of cases. Respiratory syncytial infections were diagnosed with the highest frequency (50%) in patients with BA. Influenza A(H1N1)pdm09 and adenoviral infections dominated in persons with ChBO in 50 and 42% of cases, respectively. Response of virus-specific IgG in patients with BA and ChBO indicates the acute course of influenza A(H1N1)pdm09 (63% of seroconversions). There were no reactions of IgG which is specific to respiratory syncytial virus in 75% of cases and to adenovirus in 83% of cases, that is the risk factor for occurrence of latent/persistent infection. Presence of structural components of respiratory syncytial virus in the upper respiratory tract had been revealed in three patients with asthma within at least 21–28 days. Respiratory syncytial viral infections and pandemic influenza A(H1N1) pdm09 in patients with BA and ChBO are characterized by the presence of an allergic component, which is indicated by the high levels of virus-specific IgE in blood. An adenoviral infection, in contrast, has no such peculiarity.
oai:iimmun.ru:article/447
2023-08-06T17:26:44Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/447
2023-08-06T17:26:44Z
Russian Journal of Infection and Immunity
Vol 6, No 4 (2016); 353-358
MEASLES VIRUS IMMUNITY LEVEL STUDY IN PARTICULAR POPULATION GROUPS OF THE REPUBLIC OF GUINEA WITHIN THE FRAMEWORK OF GLOBAL MEASLES ELIMINATION PROGRAM. REPORT 1
Popova A.Y.; Russian Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing (Rospotrebnadzor), Mosсow, Russian Federation
Bichurina M.A.; St. Petersburg Pasteur Institute, St. Petersburg, Russian Federation
Lavrentyeva I.N.; St. Petersburg Pasteur Institute, St. Petersburg, Russian Federation
Zheleznova N.V.; St. Petersburg Pasteur Institute, St. Petersburg, Russian Federation
Antipova A.Y.; St. Petersburg Pasteur Institute, St. Petersburg, Russian Federation
Shcherbakova S.A.; Russian Scientific and Research Antiplague Insititute “Mikrob”, Saratov, Russian Federation
Boiro M.Y.; Institute of Applied Biology in Guinea, Kindia, Republic of Guinea
Totolian A.A.; St. Petersburg Pasteur Institute, St. Petersburg, Russian Federation
2017-01-05 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/447
корь; Гвинейская Республика; заболеваемость; уровень IgG-антител; программа элиминации; лихорадка Эбола
Array
ru
Measles remains one of the main reasons for child mortality in developing countries and periodically leads to the emergence of large outbreaks in different countries. This problem became especially urgent after WHO accepted the strategic plan to fight against measles. The plan has set the goal to decrease measles on a global scale. In 2010–2011 the large outbreaks of measles were registered on the African continent: in the Democratic Republic of Congo in the south of Africa, in Nigeria and in some other African countries. In the Republic of Guinea vaccination against measles is carried out singly to children aged 9 months. In 2014–2015 the increase of measles incidence was noted. Materials and methods. Using ELISA 22 blood serum samples of healthy adult Guineans aged 24–71 and 136 blood serum samples received from children and adults — the patients of hospital in the town of Kindi (Republic of Guinea) have been examined. The clinical samples were received in 2015–2016. The following test systems were used: the test systems produced by Euroimmun Medizinische Labordiagnostika AG (Germany): «Anti-Measles Virus ELISA (IGM)», «Anti-Measles Virus ELISA (IgG)»; «Avidity: Anti-Measles Virus ELISA (IgG)», and also ELISA Vector-Best IgM-measles test system (Russia). Results and discussion. Only one out of 22 examined healthy individuals hasn’t revealed IgG-antibodies to measles virus. The quantitative titre test of IgG-antibodies, and also their avidity among other 21 individuals testify experiencing measles in the recent or remote past. Having examined 116 blood serum samples of hospital patients in Kindi for IgM-measles-antibodies, the measles case with a 2.5-year-old child has been retrospectively revealed. Having examined 130 blood serum samples for IgG-antibodies to measles virus, 12.3% of seronegative to measles individuals have been revealed. All examined individuals aged 23 and older were seropositive to measles virus, and 60% of them had high antibody titres. The antibodies to measles virus were absent or were defined in low titres among 76.2% of people under 22, which can demonstrate violations of planned child vaccination due to the Ebola outbreak. In order to decrease the risk of emergence of large measles outbreaks in the areas, free from Ebola virus transmission, WHO recommends to conduct mass anti-measles vaccination campaigns.
oai:iimmun.ru:article/1691
2023-08-06T17:09:15Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/1691
2023-08-06T17:09:15Z
Russian Journal of Infection and Immunity
Vol 11, No 6 (2021); 1095-1100
The profile of lactate, albumin, and lactate/albumin ratio as predictors of mortality in sepsis patients
https://iimmun.ru/iimm/article/download/1691/10535
Iskandar A.; Universitas Brawijaya; Dr. Saiful Anwar General Hospital
Vincentia M.I.; Airlangga University
Jaya W.; Universitas Brawijaya; Dr. Saiful Anwar General Hospital
Aryati A.; Airlangga University; Dr. Soetomo General Hospital
Pramadhani A.; Universitas Brawijaya
Aprilia A.; Universitas Brawijaya
2021-10-07 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1691
лактат; альбумин; соотношение «лактат/альбумин»; смертность; сепсис; прогноз
We would like to thank the Ministry of Research and Higher Education and Rector of the Universitas Brawijaya for providing funding for this research. We also thank our colleagues from the Department of Anesthesiology and Intensive Therapy for collaboration.
en
Background. Oxygenation disturbances in sepsis patients may cause lactate levels increase which is proportional to the severity of the inflammation, followed by decrease in albumin levels. Combination of these two parameters is expected to be predictor of mortality in patients with sepsis. The aim of this study is to investigate the profile of lactate, albumin, and lactate/albumin ratio as mortality predictors in patient with sepsis. Methods. This prospective cohort study was conducted in the ICU of dr. Saiful Anwar Hospital, Malang, from January to May 2019. Subjects were 82 patients with sepsis (SOFA score > 2). Lactate and albumin levels were measured on the first day of hospitalization. Lactate levels were examined by colorimetric method, albumin was examined by BCG method. The instrument used was Cobas 501. Comparation was carried out using the T-Test/Mann–Whitney test. Prediction of mortality risk was done using relative risk (RR) determination. Results. Significant difference was observed in albumin levels between sepsis patients who survived and who died (p = 0.045). No significant differences were observed in lactate levels and lactate/albumin ratio between sepsis patients who survived and who died (p = 0.211, 0.119, respectively). Relative risks were 3.034 for lactate, 3.667 for albumin, and 4.400 for lactate/albumin ratio. Conclusion. In patients with sepsis, albumin level is the best variable in predicting mortality, followed by lactate/albumin ratio and lactate value. Further study that implements repeated measurement of lactate and albumin in 6 and 12 hours is required to better predict the mortality of sepsis patients.
oai:iimmun.ru:article/1669
2023-11-19T09:12:09Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/1669
2023-11-19T09:12:09Z
Russian Journal of Infection and Immunity
Vol 12, No 2 (2022); 366-372
The epidemiological and clinical study of patients with <i>clostridium difficile</i> enterocolitis in Varna, Bulgaria
https://iimmun.ru/iimm/article/download/1669/10285
https://iimmun.ru/iimm/article/download/1669/10286
https://iimmun.ru/iimm/article/download/1669/10287
https://iimmun.ru/iimm/article/download/1669/10288
https://iimmun.ru/iimm/article/download/1669/10289
https://iimmun.ru/iimm/article/download/1669/10290
https://iimmun.ru/iimm/article/download/1669/10291
https://iimmun.ru/iimm/article/download/1669/10292
https://iimmun.ru/iimm/article/download/1669/10293
https://iimmun.ru/iimm/article/download/1669/10294
https://iimmun.ru/iimm/article/download/1669/10295
https://iimmun.ru/iimm/article/download/1669/10296
https://iimmun.ru/iimm/article/download/1669/10863
https://iimmun.ru/iimm/article/download/1669/10864
https://iimmun.ru/iimm/article/download/1669/11831
https://iimmun.ru/iimm/article/download/1669/11832
https://iimmun.ru/iimm/article/download/1669/11833
https://iimmun.ru/iimm/article/download/1669/11834
https://iimmun.ru/iimm/article/download/1669/11835
https://iimmun.ru/iimm/article/download/1669/11836
https://iimmun.ru/iimm/article/download/1669/11837
https://iimmun.ru/iimm/article/download/1669/11838
https://iimmun.ru/iimm/article/download/1669/12643
https://iimmun.ru/iimm/article/download/1669/12677
https://iimmun.ru/iimm/article/download/1669/12678
https://iimmun.ru/iimm/article/download/1669/12679
https://iimmun.ru/iimm/article/download/1669/12680
https://iimmun.ru/iimm/article/download/1669/12681
https://iimmun.ru/iimm/article/download/1669/12682
https://iimmun.ru/iimm/article/download/1669/12683
https://iimmun.ru/iimm/article/download/1669/12684
https://iimmun.ru/iimm/article/download/1669/12685
https://iimmun.ru/iimm/article/download/1669/12686
https://iimmun.ru/iimm/article/download/1669/13392
https://iimmun.ru/iimm/article/download/1669/13393
https://iimmun.ru/iimm/article/download/1669/13394
https://iimmun.ru/iimm/article/download/1669/13395
Lyutsova E.D.; Medical University Prof. Dr. Paraskev Stoyanov
Gospodinova M.D.; Medical University Prof. Dr. Paraskev Stoyanov
2022-05-13 14:39:38
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1669
Clostridium difficile; CDI; энтероколит; факторы риска; коморбидность; ATLAS
Array
ru
Introduction. Clostridium difficile infections (CDI) remain a global health concern. Currently, no unified approach to the diagnostics and determining severity of these infections despite their high urgency throughout the world was proposed. The aim of the study is to identify risk factors for CDI, investigate clinical and epidemiological features of the disease course and potential for using the ATLAS scale to assess its severity. Materials and methods. 36 CDI patients hospitalized at the Infectious Disease Clinic of Varna were analyzed during the period from January 2018 until June 2019. Clinical and epidemiologic study was conducted. The diagnosis was made by using a rapid immunochromatographic test; CDI patient stratification was performed by ATLAS scoring system. Results and discussion. Within the aforementioned period, 1100 patients were hospitalized at the Infectious Disease Clinic of Varna, and CDIs were reported in 3,3% of cases. The most affected were elderly individuals (the mean age was 69,8±16,4 years old) most of whom were females (92%) with only 6% of males. The following risk factors were investigated: comorbidities — 32 patients (88,89%), recent hospitalization — 19 patients (52,78%), antibiotic use — 31 patients (86,11%). Twenty-four patients (66,67%, ATLAS score ≤ 4 points) had mild CDI, whereas moderate form of CDI was observed in 12 patients (33,33%). No severe CDI or death were observed. The characteristic clinical presentation included fever, diarrhea and abdominal cramping. The treatment was implemented according to the national and international recommendations by using oral Metronidazole for 11 patients (30,56%), Vancomycin — for 12 patients (33,33%), or both — for 13 patients (36,11%). Conclusion. Patients at risk with symptoms of enterocolitis and a history of antibiotic use or hospitalizations should be screened for the presence of toxin-forming strains of Clostridium difficile. According to the European Centre for Disease Prevention (ECDC) the diagnostic yield of CDI may be increased by using two-step protocol, whereas the ATLAS score system may be a useful tool for routine evaluation of patients with CDI.
oai:iimmun.ru:article/1272
2022-04-12T15:57:53Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/1272
2022-04-12T15:57:53Z
Russian Journal of Infection and Immunity
Vol 11, No 4 (2021); 683-691
Characterization of toxigenic Corynebacterium diphtheriae strains isolated in Russia
https://iimmun.ru/iimm/article/download/1272/6763
https://iimmun.ru/iimm/article/download/1272/6765
https://iimmun.ru/iimm/article/download/1272/6766
https://iimmun.ru/iimm/article/download/1272/6767
https://iimmun.ru/iimm/article/download/1272/6768
https://iimmun.ru/iimm/article/download/1272/6769
https://iimmun.ru/iimm/article/download/1272/6770
https://iimmun.ru/iimm/article/download/1272/6771
https://iimmun.ru/iimm/article/download/1272/6772
https://iimmun.ru/iimm/article/download/1272/10758
Borisova O.Y.; G.N. Gabrichevsky Research Institute for Epidemiology and Microbiology; Russian National Research Medical University named after N.I. Pirogov
Gadua N.T.; G.N. Gabrichevsky Research Institute for Epidemiology and Microbiology
Pimenova A.S.; G.N. Gabrichevsky Research Institute for Epidemiology and Microbiology
Chaplin A.V.; G.N. Gabrichevsky Research Institute for Epidemiology and Microbiology; Russian National Research Medical University named after N.I. Pirogov
Chagina I.A.; G.N. Gabrichevsky Research Institute for Epidemiology and Microbiology
Urban Y.N.; G.N. Gabrichevsky Research Institute for Epidemiology and Microbiology
Maksimova N.M.; G.N. Gabrichevsky Research Institute for Epidemiology and Microbiology
Korzhenkova M.P.; G.N. Gabrichevsky Research Institute for Epidemiology and Microbiology
Afanasiev S.S.; G.N. Gabrichevsky Research Institute for Epidemiology and Microbiology
Kafarskaya L.I.; I.M. Sechenov First Moscow State Medical University
Afanasyev M.S.; I.M. Sechenov First Moscow State Medical University
Krikun V.V.; Nizhnevartovsk District Clinical Children’s Hospital
Yakunina O.Y.; Center of Hygiene and Epidemiology in Novosibirsk Region
2020-06-08 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1272
дифтерия; C. diphtheriae; токсигенные штаммы; больные; бактерионосители; мультилокусное сиквенс-типирование
ru
The aim of the study was to characterize toxigenic strains of Corynebacterium diphtheriae by examining 12 toxigenic strains of C. diphtheriae isolated in Russia between January, 2017 to June, 2019. The morphological, toxigenic and biochemical properties of C. diphtheriae was studied. Genotyping of C. diphtheriae strains was performed using MLST and dtxR gene sequencing with subsequent phylogenetic analysis. Results. Toxigenic strains of C. diphtheriae were isolated in the Novosibirsk, Samara and Chelyabinsk Regions, the Khanty-Mansi Autonomous Okrug — Yugra as well as the Republic of Northern Ossetia — Alania. Among these strains, 5 were isolated from diphtheria patients (moderate disease found in one case, mild course — remaining patients) and 7 strains were isolated from bacterial carriers. In two cases C. diphtheriae from diphtheria patients were identified as ST25 sequence type, gravis variant; in one case — ST8 type, gravis variant; two cases — ST67 sequence type, mitis variant. In asymptomatic carriers of tox-positive C. diphtheriae strains they belonged to ST25 sequence type, gravis variant — in two cases, ST67 type, mitis variant — in four cases. A sequencing type was not identified in one case. All sequence types were widespread globally being presented by a large number of isolates in the PubMLST and characterized by a substantial amount of derivative sequence types. At the same time, they belonged to different clonal complexes and differed markedly from each other contributing to their reliable difference as assessed by MLST. Study of gene dtxR sequence diversity showed that all allelic variants were typical for the representatives of these sequence types. New alleles of gene dtxR were not revealed in strains examined. It was shown that non-synonymous substitution C440T leading to A147V amino acid substitution was found solely in one allele distributed in ST8, ST185, ST195 and ST451 types suggesting at late mutation. In contrast, the polymorphism C640A resulting in the amino acid substitution L214I was found not only in the same allele, but also in the basal tree branches indicating that isoleucine was in the ancestral sequence of the protein.
oai:iimmun.ru:article/1545
2022-04-12T15:57:39Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/1545
2022-04-12T15:57:39Z
Russian Journal of Infection and Immunity
Vol 11, No 3 (2021); 497-505
Molecular and genetic analysis of <i>Mycobacterium tuberculosis</i> population in the Vologda Region with low tuberculosis incidence
https://iimmun.ru/iimm/article/download/1545/9286
https://iimmun.ru/iimm/article/download/1545/9287
https://iimmun.ru/iimm/article/download/1545/9288
https://iimmun.ru/iimm/article/download/1545/9289
https://iimmun.ru/iimm/article/download/1545/9290
https://iimmun.ru/iimm/article/download/1545/9291
https://iimmun.ru/iimm/article/download/1545/9292
https://iimmun.ru/iimm/article/download/1545/9293
https://iimmun.ru/iimm/article/download/1545/9294
https://iimmun.ru/iimm/article/download/1545/9295
https://iimmun.ru/iimm/article/download/1545/9296
https://iimmun.ru/iimm/article/download/1545/9297
https://iimmun.ru/iimm/article/download/1545/10381
https://iimmun.ru/iimm/article/download/1545/10609
Vyazovaya А.A.; St. Petersburg Pasteur Institute
Lebedeva I.A.; Tuberculosis Dispensary of the Vologda Region
Ushakova N.B.; Tuberculosis Dispensary of the Vologda Region
Pavlov V.V.; Tuberculosis Dispensary of the Vologda Region
Gerasimova A.A.; St. Petersburg Pasteur Institute
Solovieva N.S.; Research Institute of Phthisiopulmonology
Zhuravlev V.Y.; Research Institute of Phthisiopulmonology
Narvskaya O.V.; St. Petersburg Pasteur Institute; Research Institute of Phthisiopulmonology
2021-01-28 16:03:09
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1545
Mycobacterium tuberculosis; множественная лекарственная устойчивость; сполиготипирование; MIRU-VNTR; генотип Beijing; кластер Central Asian/Russian; кластер B0/W148
Исследование выполнено при финансовой поддержке РНФ № 19-15-00028.
ru
The Vologda Region is characterized by a relatively calm epidemic situation for tuberculosis in Russia: the incidence rate in 2010—2018 is decreased from 45.2 to 15.8 per 100 thousand of the population (44.4 in Russia). However, the proportion of patients with multiple drug resistance (MDR) of the pathogen increased from 12.1% in 2016 to 23.7% in 2018. The aim of the study was to characterize the genetic structure of the M. tuberculosis population and identify the main genotypes associated with the primary multidrug resistance of the pathogen in the Vologda Region. A total of 82 strains of M. tuberculosis isolated in 2018 from newly diagnosed tuberculosis patients were studied. Drug susceptibility testing was performed using the standard method of absolute concentration and BACTEC MGIT 960 kit. M. tuberculosis strains were assigned to the Beijing genotype and its main subtypes based on the analysis of specific markers. The Beijing strains were subtyped by the MIRU-VNTR method (24 standard loci), calculating the Hunter-Gaston Discriminatory Index (HGDI). Other strains of the non-Beijing group were spoligotyped. The majority of the strains were of the Beijing genotype (62.2%; 51 of 82). The most numerous cluster was Central Asian/Russian (41.5%; 34 of 82 strains). The shares of the Central Asia Outbreak (CAO) subtype and cluster B0/W148 amounted to 8.5% and 7.3%, respectively. The non-Beijing strains belonged to the genetic families T (11%; 9 of 82), LAM (11%), Haarlem (6.1%), and Ural (4.9%). Among 82 M. tuberculosis isolates, 33 (40.2%) MDR strains were identified, counting 27 of the Beijing genotype, including those of the Central Asian/Russian — 18 (66.7%), B0/W148 and CAO — 4 each (14.8%) clusters. MIRU-VNTR typing of 51 Beijing strains revealed 22 profiles (HGDI = 0.852); the largest clusters were 94-32 (35.3%) and 95-32 (15.7%), which included strains Central Asian/Russian and CAO. Four strains of genotype B0/W148 belonged to cluster 100-32. The loci QUB26 (HGDI = 0.493) and MIRU26 (HGDI = 0.388) had the highest polymorphism. For the first time, a molecular genetic study carried out in the Vologda region revealed the heterogeneity of the M. tuberculosis population with strains of the Beijing genotype dominated. At the same time, the share of the associated with MDR, epidemiologically and clinically significant cluster Beijing B0/W148, well defined in Russia and abroad, was only 7.3%, which is significantly less than in other regions of the Northwestern Federal District of the Russian Federation (~19%). Concurrent, representatives of the Central Asian/Russian cluster of the Beijing genotype prevailed in the structure of genotypes and among MDR M. tuberculosis strains.
oai:iimmun.ru:article/745
2022-04-12T15:47:25Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/745
2022-04-12T15:47:25Z
Russian Journal of Infection and Immunity
Vol 9, No 3-4 (2019); 504-522
Intestinal microbiota composition and peripheral blood Th cell subsets in patients with multiple sclerosis
https://iimmun.ru/iimm/article/download/745/3999
https://iimmun.ru/iimm/article/download/745/4000
https://iimmun.ru/iimm/article/download/745/4001
https://iimmun.ru/iimm/article/download/745/4002
https://iimmun.ru/iimm/article/download/745/4003
https://iimmun.ru/iimm/article/download/745/4004
https://iimmun.ru/iimm/article/download/745/4005
https://iimmun.ru/iimm/article/download/745/4006
https://iimmun.ru/iimm/article/download/745/4007
https://iimmun.ru/iimm/article/download/745/4008
https://iimmun.ru/iimm/article/download/745/4009
https://iimmun.ru/iimm/article/download/745/4014
https://iimmun.ru/iimm/article/download/745/4017
https://iimmun.ru/iimm/article/download/745/4018
https://iimmun.ru/iimm/article/download/745/4019
Abdurasulova I.N.; Institute of Experimental Medicine;
St. Petersburg State Pediatric Medical University
Tarasova E.A.; Institute of Experimental Medicine
Kudryavtsev I.V.; Institute of Experimental Medicine;
Pavlov First St. Petersburg State Medical University
Negoreeva I.G.; Institute of the Human Brain RAS
Ilves A.G.; Institute of the Human Brain RAS
Serebriakova M.K.; Institute of Experimental Medicine
Ermolenko E.I.; Institute of Experimental Medicine
St. Petersburg State University
Ivashkova E.V.; Institute of the Human Brain RAS
Matsulevich A.V.; Institute of Experimental Medicine
Tatarinov A.E.; Institute of Experimental Medicine
Stoliarov I.D.; Institute of the Human Brain RAS
Klimenko V.M.; Institute of Experimental Medicine
Suvorov A.N.; Institute of Experimental Medicine;
St. Petersburg State University
2019-11-15 09:39:48
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/745
рассеянный склероз;экспериментальный аутоиммунный энцефаломиелит;дисбиоз;кишечная микробиота;иммунорегуляция;Т-хелперы;DP Th17
en
At present, the role of intestinal microbiota in diverse diseases of the central nervous system, including of multiple sclerosis (MS) has been extensively investigated. Self-reactive CD4+ Th1 and Th17 cells specific to myelin-derived antigens play a key role in the MS pathogenesis. Taking into consideration pathogenetic features related to MS development, we examined a relation between intestinal microbiocenosis and abundance of various peripheral blood helper T (Th) cell subsets in MS patients. Objective of the study: to assess prevalence of individual members of the intestinal microbiota in MS patients and analyze a relation with peripheral blood Th cell subsets. Prevalence of symbiotic and opportunistic microbial species was estimated by bacteriological method and real time PCR in 112 MS patients (72 females, 40 males) of varying severity and duration. Th cell subsets (Th1, Th2, Th17, Th1/Th17, Th17/Th22, DP Th17) were analyzed by using multi-color flow cytometry based on Th cell subset-specific surface expression of chemokine receptors. A relationship between individual intestinal microbiota species and severity, duration and rate of MS progression, as well as with the phenotype of immune cells was assessed. It was found that the most significant correlation between percentage of peripheral blood Th cell subsets was observed with prevalence of Lactobacillus spp., Enterococcus spp. and Enterobacter spp. Moreover, prevalence of Enterococcus spp. Th cell composition influenced synergistically or antagonistically together with Enterobacter spp. or Lactobacillus spp., respectively. It is suggested that direct and indirect impact of intestinal microbiota composition on human immune system might contribute to developing novel strategies for treating MS.
oai:iimmun.ru:article/1344
2023-08-06T17:11:30Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/1344
2023-08-06T17:11:30Z
Russian Journal of Infection and Immunity
Vol 10, No 1 (2020); 137-144
Formation of humoral and cellular immunity to measles vaccine in adults
https://iimmun.ru/iimm/article/download/1344/7313
https://iimmun.ru/iimm/article/download/1344/7314
https://iimmun.ru/iimm/article/download/1344/7315
https://iimmun.ru/iimm/article/download/1344/7316
https://iimmun.ru/iimm/article/download/1344/7317
https://iimmun.ru/iimm/article/download/1344/7318
https://iimmun.ru/iimm/article/download/1344/7319
https://iimmun.ru/iimm/article/download/1344/7320
https://iimmun.ru/iimm/article/download/1344/7321
Toptygina A.P.; G.N. Gabrichevsky Research Institute for Epidemiology and Microbiology; Lomonosov Moscow State University
Andreev Y.Y.; G.N. Gabrichevsky Research Institute for Epidemiology and Microbiology
Smerdova M.A.; G.N. Gabrichevsky Research Institute for Epidemiology and Microbiology
Zetkin A.Y.; Main Center of State Sanitary and Epidemiological Supervision (of special purpose) of the Ministry of Defense of the Russian Federation
Klykova T.G.; Main Center of State Sanitary and Epidemiological Supervision (of special purpose) of the Ministry of Defense of the Russian Federation
2020-04-04 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1344
корь; антитела; специфический клеточный иммунитет; вакцинация взрослых
ru
Despite adherence to the policy of mass measles vaccination in the majority of countries, this infection still remains far from being fully eradicated. Measles outbreaks are reported worldwide, when the vast majority of cases are recorded in subjects of 18—35 years of age. Studies on assessing measles IgG antibody level in different regions of Russia reveal increased percentage of measles seronegative subjects among young adults. Current study was aimed at investigating formation of humoral and cellular immunity after measles vaccination in seronegative adults aged 18 to 30 years old. There were enrolled 50 measles seronegative healthy volunteers aged 18 to 30 years old. Level of anti-measles IgM and IgG antibodies was measured by ELISA (Vector-Best, Russia). Subclasses of measles specific IgG antibodies were analyzed by ELISA, by replacing IgG conjugate for IgG1, IgG2, IgG3, IgG4 conjugates, whereas measles specific IgA antibodies were estimated by ELISA with IgA conjugate (Polygnost, Russia) at a concentration of 1 μg/ml. Antibody avidity was assessed by ELISA (Euroimmun, Germany). Cell-mediated measles immunity was estimated by CD107a surface expression on CD8hi T cell subset stimulated by measles virus-derived antigens. A specific cellular response to measles antigens before vaccination was detected in 50% of examined subjects, whereas 40% samples showed no signs of cellular immune response, with 10% of remaining cases described as equivocal. It was found that 6 weeks after vaccination all vaccinated subjects developed measles specific IgG antibodies at protective level reaching 1.33 (0.85—1.82) IU/ml [Me (LQ—UQ)]. Anti-measles IgA antibodies were of 0.655 (0.423—1.208) IU/ml [Me (LQ—UQ)]. However, no measles specific IgM antibodies were detected 6 weeks after vaccination. In addition, primary type of immune response (dominant low-avidity anti-measles antibodies IgG3 subclass) to measles vaccination was observed in 24 out of 50 subjects, whereas 26 subjects developed secondary type of immune response (high-avidity anti-measles antibodies dominated by IgG1 subclass). A measles specific cellular immune response was observed in 47 of the 50 examined subjects, and in 3 volunteers it was equivocal. Further analysis revealed a cohort of subjects who were not vaccinated against measles (18 subjects), although 60% of them provided medical record on previous dual measles vaccination occurred in childhood. Another cohort consisted of subjects who had medical record of measles vaccination in childhood (32 subjects), but lost protective measles antibodies produced by plasma cells (23 subjects), and memory T cells (3 subjects), or measles antibodies and memory B cells (6 subjects) over time. Such pattern evidences that measles-specific cellular and humoral arms immune responses were developed and maintained independently of each other.
oai:iimmun.ru:article/8047
2023-12-15T21:01:36Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/8047
2023-12-15T21:01:36Z
Russian Journal of Infection and Immunity
Vol 13, No 4 (2023); 663-674
Features of NK cell phenotype virus genotype-driven chronic viral hepatitis C
https://iimmun.ru/iimm/article/download/8047/73857
https://iimmun.ru/iimm/article/download/8047/73858
https://iimmun.ru/iimm/article/download/8047/73859
https://iimmun.ru/iimm/article/download/8047/73860
https://iimmun.ru/iimm/article/download/8047/0
https://iimmun.ru/iimm/article/download/8047/73861
https://iimmun.ru/iimm/article/download/8047/73862
https://iimmun.ru/iimm/article/download/8047/73863
https://iimmun.ru/iimm/article/download/8047/73864
https://iimmun.ru/iimm/article/download/8047/73865
https://iimmun.ru/iimm/article/download/8047/73866
https://iimmun.ru/iimm/article/download/8047/132161
Savchenko A.A.; Federal Research Center “Krasnoyarsk Science Center” of the Siberian Branch of the Russian Academy of Sciences, Scientific Research Institute of Medical Problems of the North
Tikhonova E.P.; Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University of the Ministry of Healthcare of the Russian Federation
Anisimova A.A.; Krasnoyarsk Interdistrict Clinical Emergency Hospital named after N.S. Karpovich
Kudryavtsev I.V.; Institute of Experimental Medicine
Belenjuk V.D.; Federal Research Center “Krasnoyarsk Science Center” of the Siberian Branch of the Russian Academy of Sciences, Scientific Research Institute of Medical Problems of the North
Borisov A.G.; Federal Research Center “Krasnoyarsk Science Center” of the Siberian Branch of the Russian Academy of Sciences, Scientific Research Institute of Medical Problems of the North
2023-10-24 14:50:18
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/8047
chronic viral hepatitis C; hepatitis C virus; genotype; NK cells; phenotype; subsets; treatment
Array
ЕГИСУ 121022600088-4
en
Elimination of the hepatitis C virus (HCV) due to direct antiviral drug (DAD) action affects alteration in virus phenotype and, accordingly, NK cell functional activity. However, the published data are very contradictory. The aim of the study was to investigae alterations in NK cell subset phenotype after DAD treatment of HCV genotype-dependent chronic viral hepatitis C (CVHC) patients. Materials and methods. 111 CVHC patients and 21 healthy volunteers were examined. The diagnosis was established on epidemiological, clinical and laboratory data. All 111 subjects with CVHC received direct antiviral drugs Sofosbuvir and Velpatasvir for 12 weeks. The study of the NK cell phenotypes wwas analyzed by multicolor flow cytometry. Results. A decreased count of cytokine-producing along with increased frequency of cytotoxic NK cells were found in CVHC patients blood samples with various HCV genotypes prior to DAD treatment. The imbalance of cytotoxic cells with a high level of functional activity was also found in CVHC patients regardless of HCV genotype. The patients with HCV genotypes 1 and 3 showed significantly increased level of immunoregulatory NK cells. In addition, increased count of glycohydrolase (CD38) and ecto-5'-nucleotidase (CD73)-expressing NK cells were found in patients with HCV genotypes 1 and 3. Hence, such alterations in NK cell phenotype in CVHC patients were presented as sustained high viral load which peaking at carriers of HCV genotype 1 that was minimal in patients with HCV genotype 2. The most prominent change in NK cells after DAD treatment was found in CVHC patients with HCV genotype 2 (normalization of CD8-expressing NK cell subset composition and count). Only patients with HCV genotype 2 after treatment had increased frequencies of peripheral blood double-negative CD38–CD73– NK cells. Patients with HCV genotypes 1 and 3 also showed minimally improved in NK cell subset composition after DAD treatment. Conclusion. Evaluation of specific changes in NK cell phenotype during DAD treatment of CVHC patients driven by HCV genotype undoubtedly is of importance and high relevance. The results obtained are novel and complement the insights into CVHC immunopathogenesis. Analysis of NK cell phenotypes and functional activity in patients with CVHC may promote development of new methods for treating HCV infection.
oai:iimmun.ru:article/511
2022-04-12T15:19:55Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/511
2022-04-12T15:19:55Z
Russian Journal of Infection and Immunity
Vol 7, No 2 (2017); 117-122
PRODUCTION OF HYBRID RECOMBINANT PROTEIN Flu-Chim, CONTAINING INFLUENZA VIRUSES A AND B MAJOR EPITOPES
Dukhovlinov I.V.; LLC ATG Service-Gene
Dobrovolskaia O.A.; Institute of Experimental Medicine
Orlov A.I.; LLC Universal Biosystems
2017-06-18 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/511
вирус гриппа;универсальная вакцина;рекомбинантный белок;гемагглютинин;иммуногенность;хроматография
ru
The influenza virus is highly contagious diseases of people, birds and mammals. Approximately 250 000– 500 000 deaths are caused by influenza epidemics worldwide yearly, and the death number may be up to millions in a possible influenza pandemic. Vaccination is the most cost-effective way to reduce the considerable disease burden of seasonal influenza. Although seasonal influenza vaccines are effective, their performance in the elderly and immunocompromised individuals would benefit from improvement. Major problems related to the development and production of pandemic influenza vaccines are response time and production capacity as well as vaccine efficacy and safety. Reverse genetics techniques can speed up the generation of seed viruses and new mathematical modelling methods improve vaccine strain selection. Using vaccines based on recombinant proteins, we avoid the risks associated with the introduction of the virus into the body, even inactivated. In this paper, we have got a highly purified recombinant fusion protein composed of fragments of the hemagglutinin of influenza viruses A and B. As adjuvant we used components of flagellin. We used the most immunogenic and conserved areas of hemagglutinin H1, H3, H5 and B, which cause the formation of specific antibodies which can cross-react with homologous epitopes among the various strains of influenza A and B. Vaccine efficacy is increased by using multiple epitopes of various proteins. The aim of this study was to clone and express the hybrid recombinant protein Flu-Chim, containing immunogenic epitopes of influenza A/H1N1, A/H3N2, A/H5N1 and B fused with fragments of flagellin in Escherichia coli expression system and its subsequent purification. During the study was created high-yield E. coli strain, which produces the recombinant protein Flu-Chim, selected the optimal protocol of induction of the gene encoding the protein. The protein was purified using metal affinity chromatography. The purity of the final preparation reached 98%. In the future, we are going to study the immunogenic properties of the protein and use it as a component of the candidate vaccine against influenza.
oai:ojs.iimmun.ru:article/305
2016-02-16T11:09:36Z
iimm:ORIG
oai:iimmun.ru:article/15628
2024-02-17T14:59:08Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/15628
2024-02-17T14:59:08Z
Russian Journal of Infection and Immunity
Vol 13, No 6 (2023); 1150-1160
Side effects following administration of the Gam-COVID-Vac in Montenegro
https://iimmun.ru/iimm/article/download/15628/154867
https://iimmun.ru/iimm/article/download/15628/154868
https://iimmun.ru/iimm/article/download/15628/154869
https://iimmun.ru/iimm/article/download/15628/154873
https://iimmun.ru/iimm/article/download/15628/157962
https://iimmun.ru/iimm/article/download/15628/158003
https://iimmun.ru/iimm/article/download/15628/158004
https://iimmun.ru/iimm/article/download/15628/158005
https://iimmun.ru/iimm/article/download/15628/158006
https://iimmun.ru/iimm/article/download/15628/158009
https://iimmun.ru/iimm/article/download/15628/158024
https://iimmun.ru/iimm/article/download/15628/158726
https://iimmun.ru/iimm/article/download/15628/158727
https://iimmun.ru/iimm/article/download/15628/158728
https://iimmun.ru/iimm/article/download/15628/158729
https://iimmun.ru/iimm/article/download/15628/158730
Dabanovic V.; Pharmacy Institution of Montenegro “Montefarm” Podgorica, Montenegro
2023-12-25 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/15628
COVID-19; вакцинация; Гам-KОВИД-Вак; побочные эффекты; сопутствующие заболевания; безопасность
Array
en
Introduction. In Montenegro, vaccination against COVID-19 infection began with the use of Gam-COVID-Vac, which was not approved for emergency use before the end of clinical trials, by the Food and Drug Administration and the European Medicines Agency. Therefore, it is necessary to emphasize the adverse effects.
Materials and methods. For the purpose of this study, there were collected data from national adverse events reporting form for Gam-COVID-Vac obtained from the Health Institution Pharmacy of Montenegro — Montefarm, as the holder of permits for these vaccines. Results. For the period March 1, 2021 to February 13, 2022, after administration of 16 756 doses of vaccine Gam-COVID, a total of 220 case reports, or 716 adverse effects were recorded. The mean age of vaccinated individuals who reported adverse effects was 40.79±11.35 years. Totally, 79.55% females versus 20.45% males reported side effects post-vaccination. The most common adverse reaction was pyrexia (79.55%). Other very common adverse effects were as follows: injection site pain (38.18%), headache (33.18%), myalgia (32.27%), malaise (31.82%), fever (30.45%), arthralgia (22.73%) as well as swelling and redness at the site of application (15.91%). Less common adverse effects were nausea, pain in extremity, diarrhea, dizziness, fatigue, sore throat and labial herpes. Serious adverse effects were recorded in 8 cases including tinnitus, thrombophlebitis, hypotension, chest pain, palpitations and peripheral cyanosis related to specific comorbidities.
Conclusions. After the administration of Gam-COVID vaccine, the population in Montenegro experienced mild to moderate adverse effects, with rare serious transient adverse effects related to specific comorbidities. The data presented here on investigating Gam-COVID-Vac caccine verified good safety profile and high tolerability evidenced by the statistics analysis as lacked COVID-19-associated hospitalizations or deaths.
oai:iimmun.ru:article/736
2022-04-12T15:22:13Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/736
2022-04-12T15:22:13Z
Russian Journal of Infection and Immunity
Vol 8, No 2 (2018); 175-186
THE GROWTH RATE PHENOTYPIC PROPERTY OF MYCOBACTERIUM TUBERCULOSIS CLINICAL STRAINS: DEPENDENCE ON TUBERCULOSIS LOCALIZATION, TREATMENT, DRUG SUSCEPTIBILITY
Manicheva O.A.; St. Petersburg State Research Institute of Phthisiopulmonology
Dogonadze M.Z.; St. Petersburg State Research Institute of Phthisiopulmonology
Melnikova N.N.; St. Petersburg State Research Institute of Phthisiopulmonology
Vishnevskiy B.I.; St. Petersburg State Research Institute of Phthisiopulmonology
Manichev S.A.; St. Petersburg State University
2018-09-10 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/736
Mycobacterium tuberculosis;клинические штаммы;скорость роста;лекарственная чувствительность;туберкулез легких;внелегочный туберкулез
ru
The phenotypic properties of the M. tuberculosis strains obtained from patients with pulmonary or extra-pulmonary tuberculosis are determined by a complex set of factors: the genetic characteristics of the pathogen, its ability to adapt in vivo and in vitro, the influence of the host’s immune system and chemotherapy. The growth rate as the phenotypic property is the most accessible for the study of the host-pathogen relationships at the level of host/strain population interactions. The aim of the study is to assess in vitro of the growth rate of M. tuberculosis strains isolated from patients with pulmonary and extra-pulmonary tuberculosis: untreated and treated (with surgical and non-surgical treatment) and also sensitive and resistant isolates in comparison with the reference strain H37Rv. To estimate the growth rate of 116 clinical isolates we have used the modified method originally developed by von Groll and co-authors: to get the bacteria growth curve the fluorescence intensity of growing strains (with indicator resazurin) has been measured daily for 8 days in 96- well plate. The growth rate is determined as the slope of the growth curve. The mean values of the growth rate have been calculated in the following groups of patients: 1 — untreated patients with pulmonary tuberculosis (PT), respiratory material; 2 — non-surgical treated PT patients, respiratory material; 3 — surgical treated PT patients (mainly with chronic and hyperchronic process), respiratory material; 4 — patients like in 3rd group, surgical material; 5 — bone and joint tuberculosis (BJT), surgical material. In addition, groups of sensitive and resistant strains have been examined, but there are no significant differences in growth rates. It has been obtained that the growth rate of strains isolated from the PT patients is higher than in BJT patients: it can be explained less favorable conditions for the pathogen vegetation in the BJT. In the case of a closed tuberculous lesion where the pathogen transmission to another host is impossible, then the selection of strains with the property to survive in the tissues of the osteoarticular system is impossible too, therefor it should be observed only an adaptation of the pathogen strain population to the individual host. The growth rate of isolates from untreated PT patients is higher than that of the treated ones. Comparison of the growth parameters of only MDR strains 1–5 groups to eliminate the influence of the sensitivity/resistance has resulted in the same conclusions. We suggest that the decrease in the growth rate of strains from the treated PT patients is in not only result of the treatment, but also is conditioned by adaptation of the pathogen to its external environment, which is the internal environment of the macroorganism. To confirm this assumption, the bacterial load of 1,083 diagnostic specimens grouped in a similar manner has been estimated, taking into account only MDR/XDR strains. In the group of treated patients the frequency of high bacterial load (CFU ≥ 100) reached 52.5–63.8% that shows the conserved fitness of bacteria in such patients. The mean values of the growth rate of the strain H37Rv non-adapted to the macroorganism (due to numerous passages on artificial media) are higher than in all groups of clinical strains. Thus, heterogeneity of phenotypic properties of M. tuberculosis clinical strains on the basis of growth rate has been obtained. The growth rate of M. tuberculosis clinical strains is depended on the tuberculosis localization (PT, BJT) and on the joint effect of patient treatment and pathogen adaptation to the host.
oai:iimmun.ru:article/81
2022-04-12T21:20:09Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/81
2022-04-12T21:20:09Z
Russian Journal of Infection and Immunity
Vol 2, No 3 (2012); 651-656
EPIDEMIOLOGIC CHARACTERISTICS AND IMMUNOLOGICAL ASPECTS OF PRENATAL INFECTION
Dolgih T.I.; Омская государственная медицинская академия, г. Омск
Shelev M.V.; Омская государственная медицинская академия, г. Омск
Tirskaya J.I.; Омская государственная медицинская академия, г. Омск
Belkova T.N.; Омская государственная медицинская академия, г. Омск
2014-07-02 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/81
эпидемиология; новорожденные; внутриутробные инфекции; лимфоциты; цитокины
ru
Abstract. To improve diagnostics of prenatal infections the basic obstetrics indices in Omsk region in the period of 2000–2010 years have been analyzed. It was found that perinatal mortality reduced 2.5 times (from 14.5 till 5.7 per 1000 newborns) as well as neonatal mortality (from 6.5 till 0.9 per 1000 newborns). The laboratory testing of 187 newborns (85 pairs mother-newborn) revealed monoinfection in 24% of cases and mixed infection in 7% of cases with predomination of herpes viruses (HHV-6, CMV, EBV, HSV1,2). Newborns with manifested infections had increased number of cytotoxic T-cells, intensive expression of HLA-DR antigens on monocytes and IL-8 chemokine increased production.
oai:iimmun.ru:article/1942
2023-11-19T05:26:10Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/1942
2023-11-19T05:26:10Z
Russian Journal of Infection and Immunity
Vol 12, No 6 (2022); 1123-1128
Evaluating the iatrogenic effects of polypharmacy and drug interactions in HIV-positive patients admitted to the intensive care unit: a single-center retrospective study
https://iimmun.ru/iimm/article/download/1942/13206
https://iimmun.ru/iimm/article/download/1942/13208
https://iimmun.ru/iimm/article/download/1942/13209
https://iimmun.ru/iimm/article/download/1942/13210
https://iimmun.ru/iimm/article/download/1942/13211
https://iimmun.ru/iimm/article/download/1942/13238
https://iimmun.ru/iimm/article/download/1942/13239
https://iimmun.ru/iimm/article/download/1942/13240
https://iimmun.ru/iimm/article/download/1942/13247
https://iimmun.ru/iimm/article/download/1942/14397
https://iimmun.ru/iimm/article/download/1942/15330
Emerole K.C.; Рeoples’ Friendship University of Russia (RUDN University)
Voznesenskiy S.L.; Рeoples’ Friendship University of Russia (RUDN University)
Samotolkina Е.S.; Infectious Disease Clinical Hospital No. 2
Kozhevnikova G.M.; Рeoples’ Friendship University of Russia (RUDN University)
Klimkova P.V.; Infectious Disease Clinical Hospital No. 2
2022-12-30 21:01:02
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1942
ВИЧ-инфекция; синдром приобретенного иммунодефицита; полипрагмазия; лекарственные препараты; лекарственные взаимодействия; отделение интенсивной терапии
Array
en
Background. Polypharmacy and drug interactions are of particular concern in people living with HIV/AIDS, especially those who receive antiretroviral therapy (ARVs). Polypharmacy and drug-drug interactions (DDIs) can impact the efficacy and toxicity of HIV treatment. ARVs used in HIV treatment are often prone to drug interactions if administered with other non-ARV drugs because many of them are metabolized through the cytochrome P450 system. The pharmacological management of HIV patients in the intensive care unit (ICU) is usually complex and typically involves the administration of several classes of drugs. This patient group may be at higher risk for potential DDIs due to polypharmacy in the ICU. The main objective of this study was to assess the iatrogenic effects of polypharmacy in HIV patients treated in the ICU and to describe the DDI profile between ARVs and other non-ARV medications prescribed in the ICU. Methods and materials. Between 2018 and 2020, we conducted a single-center, retrospective study evaluating the medical records of 59 HIV patients admitted to the ICU for more than 24 hours at the Infectious Disease Clinical Hospital No. 2, Moscow, Russia. We evaluated the impact of polypharmacy on renal, hepatic and haemopoietic function. The Liverpool HIV Drug Interaction database was used to identify DDIs in ART-treated HIV patients. Results. All patients received more than 5 different medications matching the definition of polypharmacy. The average number of concurrent medications prescribed was 15±6.713 (maximum — 40, minimum — 6). All drug interactions recorded were between ARVs and antibiotics: 30 cases of potential interactions in 65.5% patients who received ARV. Of such patients, 94% were exposed to at least two potential interactions. Tenofovir (TDF) and the antibiotic vancomycin underlaid the most common potential interaction (49.2%), followed by lopinavir ritonavir (LPV/RTV) and ciprofloxacin (30.3%). A significant difference in average creatinine levels was found in patients with TDF/vancomycin potential interactions (p < 0.05). Conclusion. This study demonstrated that potential DDIs frequently occur in ICU patients in line with previous investigations. It is necessary to implement collaborations among clinical pharmacologists and infectious disease/HIV specialists, as well as frequent clinical and laboratory monitoring, aimed at developing effective and actionable strategies that could reduce potential DDIs in HIV patients in the ICU.
oai:iimmun.ru:article/1250
2023-11-19T06:00:43Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/1250
2023-11-19T06:00:43Z
Russian Journal of Infection and Immunity
Vol 13, No 3 (2023); 430-438
The impact of adjuvanted and non-adjuvanted influenza vaccines on <i>in vitro</i> lymphocyte immunophenotype
https://iimmun.ru/iimm/article/download/1250/6483
https://iimmun.ru/iimm/article/download/1250/6484
https://iimmun.ru/iimm/article/download/1250/6485
https://iimmun.ru/iimm/article/download/1250/6486
https://iimmun.ru/iimm/article/download/1250/6487
https://iimmun.ru/iimm/article/download/1250/6488
https://iimmun.ru/iimm/article/download/1250/6489
https://iimmun.ru/iimm/article/download/1250/6490
https://iimmun.ru/iimm/article/download/1250/6491
https://iimmun.ru/iimm/article/download/1250/120696
https://iimmun.ru/iimm/article/download/1250/120697
https://iimmun.ru/iimm/article/download/1250/120698
https://iimmun.ru/iimm/article/download/1250/120703
https://iimmun.ru/iimm/article/download/1250/123402
https://iimmun.ru/iimm/article/download/1250/139447
Khromova E.A.; Scientific Research Institute of Vaccines and Sera named after I.I. Mechnikov
Akhmatova N.K.; Scientific Research Institute of Vaccines and Sera named after I.I. Mechnikov
Kostinov M.P.; Scientific Research Institute of Vaccines and Sera named after I.I. Mechnikov; First Moscow State Medical University named after I.M. Sechenov of the Ministry of Health of the Russian Federation (Sechenov University)
Skhodova S.A.; Scientific Research Institute of Vaccines and Sera named after I.I. Mechnikov
Stolpnikova V.N.; Scientific Research Institute of Vaccines and Sera named after I.I. Mechnikov
Vlasenko A.E.; Novokuznetsk State Institute for Further Training of Physicians — Branch Campus of the Russian Medical Academy of Continuous Professional Education of the Ministry of Healthcare of the Russian Federation
Polishchuk V.B.; Scientific Research Institute of Vaccines and Sera named after I.I. Mechnikov
Shmitko A.D.; Scientific Research Institute of Vaccines and Sera named after I.I. Mechnikov
2023-06-26 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1250
иммуноадъювантная вакцина; безадъювантная вакцина; вакцины против гриппа; иммунофенотип лимфоцитов; клеточный иммунитет; иммуногенность вакцин
Array
ru
Vaccination is the most effective method of influenza prophylaxis resulting in reduced frequency and severity of complications. Currently, for the prevention of influenza, inactivated split and subunit vaccines as the safest and promoting formation of protective level of strain-specific virus neutralizing antibodies are used. It is known that not all inactivated vaccines are effective enough for select human groups. While nowadays the level of public health is low, there is a need to improve the effectiveness of vaccines that should activate all chains of the immune system. In order to enhance intensity of influenza virus strain-specific antibody production, adjuvant vaccines exerting other mechanisms to activate humoral and cellular immunity compared to non-adjuvant vaccines have been used. The aim of the study was to examine lymphocyte immunophenotype in 27 healthy donors treated with polymer-subunit (immunoadjuvant) and non-adjuvanted split and subunit influenza vaccines.
Materials and methods. Peripheral blood lymphocyte subpopulations were studied in vitro by flow cytometer FC-500 Cytomics (Beckman Coulter, USA) using FITC- and PE-labeled monoclonal antibodies (mAbs).
Results. All examined influenza vaccines activate the effectors of cellular immunity, increasing the number of NK-cells (CD16/56), NKT-lymphocytes (CD3/CD16/56), B-lymphocytes (CD45/CD20), activated (CD3/HLA-DR) and cytotoxic (CD8/HLA-DR) T-lymphocytes, as well as cells bearing early activation marker (CD45/CD25). Among them the immunoadjuvant vaccine showed the greatest potential to induce cellular response eliciting regulatory mechanisms that prevent hyperactivation, stimulating growth of NK (CD16/56), NKT-cells (CD3/CD16/56), B-lymphocytes (CD45/CD20), activated (CD3/HLA-DR) and cytotoxic (CD8/HLA-DR) T-lymphocytes, T-regulatory cells (Tregs, CD4/CD25/Foxp3).
Conclusion. Vaccination against influenza besides the formation of specific antibodies render a transient, immunomodulating effect that is more noticeable after immunoadjuvant vaccine. It can be assumed that vaccination of people with dysfunctions of the immune system an additional prophylactic effect will be observed.
oai:iimmun.ru:article/180
2015-06-16T09:39:09Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/180
2015-06-16T09:39:09Z
Russian Journal of Infection and Immunity
Vol 4, No 3 (2014); 221-228
MYCOBACTERIUM TUBERCULOSIS BIOLOGICAL PROPERTIES AND CHARACTERISTICS OF THE INFLAMMATORY REACTION IN PATIENTS WITH INFILTRATIVE PULMONARY TUBERCULOSIS
Titarenko O.T.; St. Petersburg Research Institute of Phthisiopulmonology, St. Petersburg
Dyakova M.E.; St. Petersburg Research Institute of Phthisiopulmonology, St. Petersburg
Manicheva O.A.; St. Petersburg Research Institute of Phthisiopulmonology, St. Petersburg
Esmedlyaeva D.S.; St. Petersburg Research Institute of Phthisiopulmonology, St. Petersburg
Dogonadze M.Z.; St. Petersburg Research Institute of Phthisiopulmonology, St. Petersburg
Alexeyeva N.P.; St. Petersburg Research Institute of Phthisiopulmonology, St. Petersburg
Perova T.L.; St. Petersburg Research Institute of Phthisiopulmonology, St. Petersburg
Melnikova N.N.; St. Petersburg Research Institute of Phthisiopulmonology, St. Petersburg
2014-08-15 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/180
туберкулез; белки острой фазы; генотип; вирулентность
ru
Host-pathogen relations were analyzed on basis of the results of examinations of 42 patients with newly diagnosed, previously untreated infiltrative pulmonary tuberculosis (IPT) with regard for the biological characteristics of Mycobacterium tuberculosis (MBT) and the biomarkers of the inflammatory reaction — the acute phase proteins (APPs). The genotypes of MBT (Beijing and the others) and the efficacy of threeonth antituberculosis therapy were used as the grouping factors. Genotype Beijing MBT patients were significantly often characterised by multiple drug resistant, had widespread pulmonary damage and association of MBT cytoxicity with the effect of therapy. The patients with the best postreatment effect had initial (pretreatment) APPs levels in the range of the referent ones. These conclusions were confirmed by analysis of the correlation pleads of the characteristics of the “host-pathogen” system and their discriminant analysis with regard for MBT genotypes and results of antituberculosis three-month therapy. It was shown that the constellation of four host’s APP characteristics (haptoglobin, ceruloplasmin, elastase and adenoaminase activities) has above 90% prognostic efficacy of treatment in spite of MBT genotype. It is suggested that in IPT patients the first component of the “host-pathogen” system reflecting the reaction of the patient’s organism to the MBT induced inflammatory process is more prognostically important.
oai:iimmun.ru:article/350
2023-08-06T17:26:59Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/350
2023-08-06T17:26:59Z
Russian Journal of Infection and Immunity
Vol 5, No 4 (2015); 331-338
ASSOCIATION OF TREM-1 GENE POLYMORPHISMS WITH INFECTIVE ENDOCARDITIS
Ponasenko A.V.; Research Institute for Complex Issues of Cardiovascular Diseases, Kemerovo, Russian Federation
Kutikhin A.G.; Research Institute for Complex Issues of Cardiovascular Diseases, Kemerovo, Russian Federation
Khutornaya M.V.; Research Institute for Complex Issues of Cardiovascular Diseases, Kemerovo, Russian Federation
Yuzhalin A.E.; Oxford Institute for Radiation Oncology, Oxford, UK
Rutkovskaya N.V.; Research Institute for Complex Issues of Cardiovascular Diseases, Kemerovo, Russian Federation
Golovkin A.S.; Federal North-West Medical Research Centre, St. Petersburg, Russian Federation
2016-02-15 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/350
инфекционный эндокардит;генные полиморфизмы;врожденный иммунитет;TREM-1;воспалительный ответ;инфекционный иммунитет
ru
Infective endocarditis (IE) is a septic inflammation of endocardium, which generally involves the lining of the heart chambers and heart valves. The development of IE depends in many respects on how properly and efficiently the immune system responds to the occurrence of an infection. Innate immunity, which carries out the response to a transient bacteremia, is genetically determined in a large extent. Pattern recognition receptors, which identify pathogenand danger-associated molecular patterns, are the main effectors of innate immune response; one of these receptors is triggering receptor expressed on myeloid cells-1 (TREM-1). We hypothesized that inherited variation in TREM-1 gene may affect individual susceptibility to IE. The distribution of genotypes and alleles of rs1817537, rs3804277, rs6910730, rs7768162, rs2234246, rs4711668, rs9471535, and rs2234237 gene polymorphisms was investigated in 110 Caucasian (Russian) subjects with IE and 300 age-, sex-, and ethnicity-matched healthy blood donors. Odds ratios with 95% confidence intervals were calculated. We found that rs1817537 polymorphism was associated with decreased IE risk (OR = 0.60; 95%CI = 0.37–0.99; р = 0.046, dominant model); however, this was not significant after an adjustment for multiple comparisons. Therefore, we observed no statistically significant association between the investigated polymorphisms within TREM-1 gene and IE. Further in-depth investigations in this field are necessary to shed the light on the impact of inherited variation within innate immune response genes on the development of IE.
oai:iimmun.ru:article/1708
2023-11-16T17:04:26Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/1708
2023-11-16T17:04:26Z
Russian Journal of Infection and Immunity
Vol 12, No 1 (2022); 142-148
<i>In vitro</i> antiviral activity of VIFERON® rectal suppositories against SARS-CoV-2
https://iimmun.ru/iimm/article/download/1708/10656
https://iimmun.ru/iimm/article/download/1708/10657
https://iimmun.ru/iimm/article/download/1708/10658
https://iimmun.ru/iimm/article/download/1708/10659
https://iimmun.ru/iimm/article/download/1708/10660
https://iimmun.ru/iimm/article/download/1708/10661
https://iimmun.ru/iimm/article/download/1708/10662
https://iimmun.ru/iimm/article/download/1708/10663
https://iimmun.ru/iimm/article/download/1708/10664
https://iimmun.ru/iimm/article/download/1708/10665
https://iimmun.ru/iimm/article/download/1708/0
https://iimmun.ru/iimm/article/download/1708/10834
https://iimmun.ru/iimm/article/download/1708/10835
https://iimmun.ru/iimm/article/download/1708/10836
https://iimmun.ru/iimm/article/download/1708/10837
https://iimmun.ru/iimm/article/download/1708/10838
https://iimmun.ru/iimm/article/download/1708/10839
https://iimmun.ru/iimm/article/download/1708/10840
https://iimmun.ru/iimm/article/download/1708/10841
https://iimmun.ru/iimm/article/download/1708/10842
https://iimmun.ru/iimm/article/download/1708/10843
https://iimmun.ru/iimm/article/download/1708/12458
Isakova-Sivak I.N.; Institute of Experimental Medicine
Stepanova E.A.; Institute of Experimental Medicine
Rudenko L.G.; Institute of Experimental Medicine
Bartov M.S.; N.F. Gamaleya Federal Research Center for Epidemiology and Microbiology
Vyzhlova E.N.; N.F. Gamaleya Federal Research Center for Epidemiology and Microbiology
Malinovskaya V.V.; N.F. Gamaleya Federal Research Center for Epidemiology and Microbiology
2022-01-20 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1708
ВИФЕРОН®; интерферон альфа; COVID-19; SARS-COV-2; Vero; in vitro
The work was financially supported by FERON LLC.
ru
In 2020–2021, the world was engulfed by the pandemic of a new coronavirus infection (COVID-19) caused by the SARS-CoV-2 virus. The low population coverage with vaccination against COVID-19 and the lack of herd immunity result in the need to find an effective and safe etiotropic treatment. Medicinal agents for treatment of COVID-19, approved while preparing this publication, have several limitations related to the conditions of their use and/or population category. In this situation, interferon-containing drugs widely used in Russia and the CIS for prevention and treatment of viral infectious diseases, i.e. ARVI and influenza, may hold promise. This study aims to confirm in vitro antiviral activity against SARS-CoV-2 for the preparation VIFERON® containing recombinant human interferon alpha-2b (IFNα-2b). Materials and methods. Vero CCL-81 cells were infected with hCoV-19/StPetersburg-RII3524VR4/2020 strain of SARS-CoV-2 at doses of 10 TCID50 or 100 TCID50 per well. The suppressive effect of IFN-2b, extracted from VIFERON® in dosage form of rectal suppositories, was evaluated by qRT-PCR at 24 h and 48 h after the infection of cells in two schemes, simulating preventive (24 h before infection) and therapeutic (2 h after infection) use of drugs. Results. IFNα-2b at concentrations of 800, 400, 200, 100 and 50 IU/ml, extracted from rectal suppositories of VIFERON®, showed high biological activity, displayed as inhibition of SARS-CoV-2 strain replication in both infectious doses evaluated either at 24 h or at 48 h after cell infection. The “preventive” vs. “therapeutic” scheme was found to be more effective. In the “preventive” scheme the virus titre decreased by more than 3 lg TCID50 at 24 hours post-infection and by 5–6 lg TCID50 at 48 hours post-infection after administration of 800 IU/ml IFNα-2b. Conclusion. The study results evidence that VIFERON® in dosage form of rectal suppositories may be promising for prevention and treatment of new coronavirus infection in clinical practice.
oai:iimmun.ru:article/1905
2023-01-17T13:08:26Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/1905
2023-01-17T13:08:26Z
Russian Journal of Infection and Immunity
Vol 12, No 4 (2022); 677-687
Diagnostics of macrophage activation syndrome, depending on IL-6 initial level in patients with a novel coronavirus infection
https://iimmun.ru/iimm/article/download/1905/12898
https://iimmun.ru/iimm/article/download/1905/12899
https://iimmun.ru/iimm/article/download/1905/12900
https://iimmun.ru/iimm/article/download/1905/12901
https://iimmun.ru/iimm/article/download/1905/12902
https://iimmun.ru/iimm/article/download/1905/12903
https://iimmun.ru/iimm/article/download/1905/12904
https://iimmun.ru/iimm/article/download/1905/12905
https://iimmun.ru/iimm/article/download/1905/12906
https://iimmun.ru/iimm/article/download/1905/12907
https://iimmun.ru/iimm/article/download/1905/12908
https://iimmun.ru/iimm/article/download/1905/13720
https://iimmun.ru/iimm/article/download/1905/14353
https://iimmun.ru/iimm/article/download/1905/14354
https://iimmun.ru/iimm/article/download/1905/14355
https://iimmun.ru/iimm/article/download/1905/14356
Perepelitsa S.A.; Imannuel Kant Baltic Federal University; Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology
2022-11-15 12:49:02
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1905
коронавирус; интерлейкины; воспалительный ответ; ферритин; пневмония; дыхательная недостаточность; селезенка; микроспления
Array
ru
Introduction. The novel coronavirus infection caused by the SARS-CoV-2 remains the main problem, which is being studied by all the efforts of the global scientific community. Large clinical recourse has been accumulated that allows to conduct more effective treatment of patients, but there are still unresolved issues on the pathogenesis for development and course of the disease.
Materials and methods. The study included 163 patients admitted to the infectious diseases hospital diagnosed with “Novel coronavirus infection caused by the SARS-CoV-2”. Upon admission, all patient serum samples were quantified for IL-6 level that allowed to stratify patients into three groups: A — 55 patients with IL-6 below 5.0 pg/ml. The mean age in the group was 57.3±14.9 years, body mass index (BMI) was 28.2±5.6 kg/m2; C — 52 patients whose serum IL-6 level was in the range of 5–49 pg/ml. The average age in the group was 60.8±11.8 years, BMI — 29.6±5.5 kg/m2; C — 56 patients in whom the level of IL-6 in the blood serum ranged within 50–300 pg/ml. The average age in the group was 62.5±15.6 years, BMI — 28.8±5.6 kg/m2. Patients at admission were analysed for serum level of IL-6, IL-8, and C-reactive protein (CRP), ferritin, lactate dehydrogenase (LDH) were also determined on day 3 and 7.
Results. The minimum production of IL-6 within the range of 0.1–5 pg/ml, corresponds to the minimum changes in IL-8, CRP, and ferritin as well as LDH that was within the range of physiological values. Moderate cytokinemia, IL-6 is within the range of 5–49 pg/ml was associated with elevated ferritin and LDH not tending to decline by the end of treatment. Significant cytokinemia, the level of IL-6 within the range of 50–300 pg/ml was associated with hyperferritinemia and increased LDH. The course of COVID-19 in such patients is characterized by increased ferritin by day 3 of treatment, consistently high level of LDH, without a significant trend towards a decline in the studied markers by the end of treatment.
Conclusion. The risk of developing macrophage activation syndrome is not observed of the serum IL-6 level was below 5 pg/ml, whereas ferritin and LDH were within the range of physiological values, with no/degree I ARF. Moderate macrophage activation syndrome is characterized by increased serum IL-6 level within the range 5–49 pg/ml, a moderate increase in LDH and ferritin, as well as signs of ARF I–II degree. Severe signs are diagnosed in case of serum IL-6 level exceeded 50 pg/ml, along with significant increase in LDH and ferritin, as well as signs of II–III degree ARF.
oai:iimmun.ru:article/640
2023-08-06T17:11:39Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/640
2023-08-06T17:11:39Z
Russian Journal of Infection and Immunity
Vol 9, No 5-6 (2019); 713-722
Dynamics in IgM and IgG antibodies against a polysaccharide capsule-containing complex of various <i>S. pneumonia</i> and <i>H. influenza</i> type b serotypes in children with chronic lung and bronchial inflammatory diseases after vaccination with “Pneumo-23” and “Act-HIB”
https://iimmun.ru/iimm/article/download/640/3268
https://iimmun.ru/iimm/article/download/640/3269
https://iimmun.ru/iimm/article/download/640/3270
https://iimmun.ru/iimm/article/download/640/3271
https://iimmun.ru/iimm/article/download/640/3272
https://iimmun.ru/iimm/article/download/640/3273
https://iimmun.ru/iimm/article/download/640/3274
https://iimmun.ru/iimm/article/download/640/3275
https://iimmun.ru/iimm/article/download/640/3276
Korovkina E.S.; I. Mechnikov Scientific-research Institute of Vaccines and Sera
Krakovskaya A.V.; Far-Eastern Scientific Center of Physiology and Pathology of Respiration — Research Institute of Motherhood and Childhood Protection
Kostinov M.P.; I. Mechnikov Scientific-research Institute of Vaccines and Sera; Sechenov First Moscow State Medical University
Kozlov V.K.; Far-Eastern Scientific Center of Physiology and Pathology of Respiration — Research Institute of Motherhood and Childhood Protection
Jastrebova N.E.; I. Mechnikov Scientific-research Institute of Vaccines and Sera
Magarshak O.O.; I. Mechnikov Scientific-research Institute of Vaccines and Sera
Polishchuk V.B.; I. Mechnikov Scientific-research Institute of Vaccines and Sera
2019-12-04 19:52:51
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/640
IgG; IgM; Streptococcus pneumoniae; Haemophilus influenzae типа b; вакцинопрофилактика; дети; хронические воспалительные заболевания легких; бронхиальная астма; адаптивный иммунитет
ru
A lead place among pathogens resulting in pediatric chronic bronchopulmonary diseases is held by S. pneumoniae and Haemophilus influenzae. Vaccination against pneumococcal and hemophilic infections is approved and recommended for patients with chronic pathologies, but no clear recommendations for combined use of Pneumo-23 and Act-HIB vaccines in children with congenital pulmonary malformations and bronchial asthma were proposed.Materials and methods. There were enrolled 92 children aged 0–17 years old with chronic bronchopulmonary diseases; 55 healthy children, and 57 unvaccinated children with chronic broncho-pulmonary pathology were included into control group. Mono- and combination vaccination by Pneumo-23 and/or Act-HIB was performed in remission period. IgM and IgG level against H. influenzae antigens, H. influenzae type b, to S. pneumoniae (serotypes 3, 6B, 9N, 23F) vaccine-specific polysaccharide as well as polysaccharide complex antigens were measured by using ELISA developed by us. Statistical processing was carried out by methods of descriptive, parametric and nonparametric statistics by using Statistica 5.0 software.Results. Vaccination with Pneumo 23 was accompanied by IgG production against serotypes 3, 6, 9N, 23F-derived polysaccharide. A markedly increased anti-serotype 3 and 23F antibody level was observed 6 months after vaccination. Moreover, a significant increase in anti-polysaccharide 23F and anti-vaccine-derived polysaccharide IgM levels was found 1 month after the onset. In addition, anti-serotype 6B and 9N IgM antibodies were maintained 18 months after vaccination at high level, whereas it was significantly elevated against serotypes 3, 23F. Assessing an effectiveness of vaccine prophylaxis against Hemophilus type b infection, it was shown that a significantly increased anti-vaccine-derived polysaccharide IgM level was found 1, 6, 18 months after Act-HIB vaccination. In addition, IgG to the anti-H. influenzae type b polysaccharide tended to rise 1 month after the vaccination.Summary. Children with chronic bronchopulmonary diseases vaccinated by Pneumo-23 and Act-HIB demonstrated activated adaptive immunity manifested by increased vaccine-derived antigen-specific IgM and IgG antibodies.
oai:iimmun.ru:article/16937
2023-12-18T11:53:29Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/16937
2023-12-18T11:53:29Z
Russian Journal of Infection and Immunity
Vol 13, No 5 (2023); 873-884
Assessment of trec and krec levels in COVID-19 patients with varying disease severity
https://iimmun.ru/iimm/article/download/16937/152712
https://iimmun.ru/iimm/article/download/16937/152713
https://iimmun.ru/iimm/article/download/16937/152714
https://iimmun.ru/iimm/article/download/16937/152715
https://iimmun.ru/iimm/article/download/16937/152726
https://iimmun.ru/iimm/article/download/16937/152727
https://iimmun.ru/iimm/article/download/16937/152728
https://iimmun.ru/iimm/article/download/16937/152729
https://iimmun.ru/iimm/article/download/16937/152736
https://iimmun.ru/iimm/article/download/16937/152742
https://iimmun.ru/iimm/article/download/16937/152743
https://iimmun.ru/iimm/article/download/16937/152744
https://iimmun.ru/iimm/article/download/16937/152745
https://iimmun.ru/iimm/article/download/16937/152746
https://iimmun.ru/iimm/article/download/16937/152747
https://iimmun.ru/iimm/article/download/16937/152748
https://iimmun.ru/iimm/article/download/16937/152759
https://iimmun.ru/iimm/article/download/16937/152760
https://iimmun.ru/iimm/article/download/16937/152761
https://iimmun.ru/iimm/article/download/16937/152763
https://iimmun.ru/iimm/article/download/16937/152764
https://iimmun.ru/iimm/article/download/16937/152765
https://iimmun.ru/iimm/article/download/16937/152766
https://iimmun.ru/iimm/article/download/16937/152767
https://iimmun.ru/iimm/article/download/16937/154198
https://iimmun.ru/iimm/article/download/16937/158141
https://iimmun.ru/iimm/article/download/16937/158142
https://iimmun.ru/iimm/article/download/16937/158143
https://iimmun.ru/iimm/article/download/16937/158144
https://iimmun.ru/iimm/article/download/16937/158145
https://iimmun.ru/iimm/article/download/16937/158146
https://iimmun.ru/iimm/article/download/16937/158147
https://iimmun.ru/iimm/article/download/16937/158148
https://iimmun.ru/iimm/article/download/16937/158149
https://iimmun.ru/iimm/article/download/16937/158150
Saitgalina M.A.; St. Petersburg Pasteur Institute
Ostankova Y.V.; St. Petersburg Pasteur Institute
Arsentieva N.A.; St. Petersburg Pasteur Institute
Korobova Z.R.; St. Petersburg Pasteur Institute
Liubimova N.E.; St. Petersburg Pasteur Institute
Kashchenko V.A.; North-Western District Scientific and Clinical Center named after L.G. Sokolov Federal Medical and Biological Agency; St. Petersburg State University
Kulikov A.N.; I.P. Pavlov First St. Petersburg State Medical University of the Ministry of Healthcare of Russian Federation
Pevtsov D.E.; I.P. Pavlov First St. Petersburg State Medical University of the Ministry of Healthcare of Russian Federation
Stanevich O.V.; I.P. Pavlov First St. Petersburg State Medical University of the Ministry of Healthcare of Russian Federation; Smorodintsev Research Institute of Influenza
Chernykh E.I.; North-Western District Scientific and Clinical Center named after L.G. Sokolov Federal Medical and Biological Agency
Totolian A.A.; St. Petersburg Pasteur Institute; I.P. Pavlov First St. Petersburg State Medical University of the Ministry of Healthcare of Russian Federation
2023-11-30 23:30:51
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/16937
TREC; KREC; COVID-19; SARS-CoV-2; иммунный статус; лабораторные маркеры
Array
ru
According to the WHO data, the number of infected people exceeded 765.2 million people during the COVID-19 pandemic. The severity of patient’s condition is determined by immune system hyperactivation. Activation of T- and B-lymphocyte subsets plays a prominent role in the control of infectious process. A content of small circular DNA molecules — T-cell receptor excision circles (TREC — T-cell receptor excision circles) and B-cell (“kappa”) excision rings (KREC — Kappa-deleting recombination excision circles) — in the peripheral blood can be used as a marker of the functionally active T and B cells maturation. The purpose of this work is to quantify peripheral blood TREC and KREC level in patients with the new coronavirus infection COVID-19 of varying severity. Materials and methods. The material consisted of 1028 blood samples from patients with a confirmed diagnosis of COVID-19, as well as 717 blood samples from apparently healthy volunteers. The content of TREC and KREC DNA fragments in the total DNA fraction was assessed by quantitative Real-time PCR using the “TREC/KREC-AMP PS” test system (St. Petersburg Pasteur Institute, Russia). Blood cell phenotyping was carried out using flow cytometry. Results. TREC/KREC levels were significantly reduced in COVID-19 patients (p < 0.0001 at 95% CI). A significant direct correlation was established between the levels of peripheral blood TREC molecules and level of CD45+CD3+CD19– T cells (r = 0.59, p < 0.0001), as well as between KREC level and count of CD45+CD3–CD19+ B cells (r = 0.66, p < 0.0001). The level of TREC molecules in patients with severe vs. moderate infection was significantly reduced in patients aged 30–39 years old (p = 0.0404) and 40–49 years old (p = 0.0356). The negative correlation between severity of COVID-19 clinical manifestations and TREC level in the blood of patients in 30–49 year age group indicates about an opportunity of using this analyte as a diagnostic and prognostic laboratory marker of patient’s condition. A simple PCR analysis algorithm makes it relevant to use the described method for assessing a state of immunity in coronavirus patients in the context of systemic negative impact of the SARS-CoV-2 virus on human organism.
oai:iimmun.ru:article/558
2022-04-12T15:20:30Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/558
2022-04-12T15:20:30Z
Russian Journal of Infection and Immunity
Vol 7, No 3 (2017); 259-270
THE PHENOTYPE AND METABOLISM RELATIONSHIP OF BLOOD NEUTROPHILS IN PATIENTS WITH WIDESPREAD PURULENT PERITONITIS IN THE POSTOPERATIVE PERIOD DYNAMICS
Savchenko A.A.; Federal Research Center «Krasnoyarsk Science Center» of the Siberian Branch of the Russian Academy of Sciences, Scientific Research Institute of Medical Problems of the North; Krasnoyarsk State Medical University named after prof. V.F. Voino-Yasenetsky
Borisov A.G.; Federal Research Center «Krasnoyarsk Science Center» of the Siberian Branch of the Russian Academy of Sciences, Scientific Research Institute of Medical Problems of the North; Krasnoyarsk State Medical University named after prof. V.F. Voino-Yasenetsky
Kudryavcev I.V.; Research Institute of Experimental Medicine; Far Eastern Federal University; Pavlov First St. Petersburg State Medical University
Gvozdev I.I.; Federal Research Center «Krasnoyarsk Science Center» of the Siberian Branch of the Russian Academy of Sciences, Scientific Research Institute of Medical Problems of the North
Moshev A.V.; Federal Research Center «Krasnoyarsk Science Center» of the Siberian Branch of the Russian Academy of Sciences, Scientific Research Institute of Medical Problems of the North
Cherdancev D.V.; Krasnoyarsk State Medical University named after prof. V.F. Voino-Yasenetsky
Pervova O.V.; Krasnoyarsk State Medical University named after prof. V.F. Voino-Yasenetsky
2017-09-28 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/558
перитонит; послеоперационный период; нейтрофильные гранулоциты; фенотип; метаболизм; активность ферментов
ru
The aim of the study was to examine the relationship of the phenotype and metabolism of neutrophils in patients with widespread purulent peritonitis (WPP) in the dynamics of the postoperative period. The study involved 27 patients with acute surgical diseases and injuries of abdominal organs complicated by WPP. Blood sampling was performed prior to surgery (pre-operative period) and at 7, 14 and 24 day post-operative period. As controls 67 respect healthy people were examined. Research blood neutrophilic granulocytes phenotype was performed by f low cytometry using a direct immunof luorescence whole peripheral blood. The levels of surface receptor expression was assessed by the mean f luorescence intensity. The NADand NADP-dependent dehydrogenases activity in the blood neutrophils studied using bioluminescence method. It was found that in patients with WPP in the preoperative period in the peripheral blood increased content of CD62L+-, HLA-DR+and CD64+-neutrophils. High levels of CD62L+-cells stored within 24 postoperative days, whereas the amount of HLA-DR+and CD64+-neutrophils on 24 postoperative day is reduced to the level of controls. The dynamics of changes in the content of CD64+-cells in the peripheral blood of patients with WPP corresponds to the expression level of CD64-receptor on the membrane of neutrophilic granulocytes. The metabolism of blood neutrophils in patients with WPP in the preand postoperative period is characterized by high intensity of the substrate stream on the citric acid cycle, low activity of NADP-dependent glutamate dehydrogenase and aerobic reaction of lactate dehydrogenase. In the preoperative period and within 14 days of the postoperative period in neutrophil granulocytes of the patients revealed high activity of anaerobic lactate dehydrogenase reaction characterized by increased activity of anaerobic glycolysis. In the late postoperative period the intensity of anaerobic energy in the neutrophils of patients with WPP was reduced to the control level. The final stage of post-operative treatment the metabolism in the blood neutrophils of patients with WPP is also characterized by the activation of the pentose phosphate cycle, low activity of malate-aspartate shunt mitochondria and high intensity of the substrate interaction between citric acid cycle and reactions of amino acid metabolism. Using correlation analysis set dysregulation between phenotype and the system of intracellular metabolism of neutrophils which can be determined by the migration of activated cells in the inf lammatory focus as well as changes in the activity of intracellular enzymes under different regulatory factors and including postoperative therapy methods peritonitis.
oai:iimmun.ru:article/1586
2022-04-12T04:49:13Z
iimm:ORIG
oai:iimmun.ru:article/788
2023-08-06T17:26:36Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/788
2023-08-06T17:26:36Z
Russian Journal of Infection and Immunity
Vol 8, No 3 (2018); 361-368
EFFICIENCY OF APPLICATION OF ISOTHERMAL AMPLIFICATION AT INSPECTION OF PATIENTS WITH WHOOPING COUGH
Pimenova A.S.; G.N. Gabrichevsky Research Institute of Epidemiology and Microbiology.
Borisova O.Y.; G.N. Gabrichevsky Research Institute of Epidemiology and Microbiology; Russian National Research Medical University named after N.I. Pirogov.
Petrova M.S.; G.N. Gabrichevsky Research Institute of Epidemiology and Microbiology.
Voronina I.S.; G.N. Gabrichevsky Research Institute of Epidemiology and Microbiology.
Borisova A.B.; G.N. Gabrichevsky Research Institute of Epidemiology and Microbiology.
Shamsheva O.V.; Russian National Research Medical University named after N.I. Pirogov.
Afanasiev S.S.; G.N. Gabrichevsky Research Institute of Epidemiology and Microbiology.
Vlasov E.V.; Infectious Diseases Clinical Hospital No. 1 of the Moscow Department of Healthcare.
Aleshkin V.A.; G.N. Gabrichevsky Research Institute of Epidemiology and Microbiology.
2018-11-02 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/788
коклюш; изотермальная амплификация; пациенты; возраст; период болезни
ru
Purpose: efficiency isothermal amplification (LAMP) at inspection of patients with whooping cough in clinical conditions. Materials and methods. Examination of 262 patients aged from 0 months up to 30 years hospitalized in Infectious diseases clinical hospital No. 1 of the Moscow Department of Healthcare is conducted. Clinical specimens (pharyngeal swabs) were collected according to MR 3.1.2.0072-13. Extraction DNA of B. pertussiswas carried out by means of the АmplyPrime® DNA-sorb-AM. Identification of specific fragments of a genome of B. pertussiswas performed by PCR-real time by means of the АmplySens ® Bordetella Multi-FL set (a comparison method) and by the LAMP with a phoresis and intercalating dye. Results.When using of the optimized method LAMP the DNA of B. pertussisis found at 252 (96.2%) patients. The method was effective at any forms of whooping cough —DNA of B. pertussisis found in all patients with a severe form, in 95.8% of cases — in patients with medium-weight and in 95.3% of cases — in patients with an easy form. The DNA of B. pertussisis found in clinical specimens received from patients on different terms from the beginning of a disease — from 92.3% on the 1st week up to 96% of cases — on the 5 th and more weeks of a disease. The DNA of B. pertussisis found in high percent of cases (96.7–95.9%) and did not depend on acceptance of antibacterial therapy. Children till 1 year are the main age group which is subject to hospitalization at suspicion of whooping cough and in which the highest risk of development of complications and severe forms of a clinical disease. At inspection of 169 children from 0 to 12 months by means of the optimized method LAMP, DNA of B. pertussisit is found in 98.6% of cases in children of patients with whooping cough aged from 0–3 months, in 98.4% of cases — children of 4–7 months and in 94.6% of cases — at children have 8–12 months. The efficiency of detection of DNA of B. pertussisat patients with whooping cough of children aged till 1 year by means of the optimized LAMP method was 97.6%.
oai:iimmun.ru:article/1981
2023-11-17T08:45:55Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/1981
2023-11-17T08:45:55Z
Russian Journal of Infection and Immunity
Vol 12, No 4 (2022); 735-744
Detection of human papillomavirus, viral load and risk factors in patients with precancerous diseases and malignant neoplasms in St. Petersburg
https://iimmun.ru/iimm/article/download/1981/13683
https://iimmun.ru/iimm/article/download/1981/13684
https://iimmun.ru/iimm/article/download/1981/13685
https://iimmun.ru/iimm/article/download/1981/13686
https://iimmun.ru/iimm/article/download/1981/13687
https://iimmun.ru/iimm/article/download/1981/13688
https://iimmun.ru/iimm/article/download/1981/13689
https://iimmun.ru/iimm/article/download/1981/13690
https://iimmun.ru/iimm/article/download/1981/13691
https://iimmun.ru/iimm/article/download/1981/13692
https://iimmun.ru/iimm/article/download/1981/13693
https://iimmun.ru/iimm/article/download/1981/14196
https://iimmun.ru/iimm/article/download/1981/14364
https://iimmun.ru/iimm/article/download/1981/14365
https://iimmun.ru/iimm/article/download/1981/14366
Kholopov D.V.; St. Petersburg Pasteur Institute
Vyazovaya A.A.; St. Petersburg Pasteur Institute
Topuzov E.E.; St. Petersburg City Oncology Clinic
Alekseeva D.A.; St. Petersburg City Oncology Clinic
Molchanov S.V.; St. Petersburg City Oncology Clinic
Lyalina L.V.; St. Petersburg Pasteur Institute
2022-11-15 12:49:12
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1981
вирус папилломы человека; злокачественные новообразования; рак шейки матки; орофарингеальный рак; анальный рак; факторы риска; вирусная нагрузка
Array
ru
Molecular genetic studies have revealed the involvement of different genotypes of human papillomavirus (HPV) in the carcinogenesis of cervical cancer and malignant lesions of other localizations. It is reported that patients with HPV-positive cancer have a better prognosis of the disease and survival than patients with unconfirmed HPV infection or with a low viral load. The objective was to identify the detectability, viral load, genotypes of human papillomavirus in HPV-associated precancerous and malignant neoplasia of various localization and to determine risk factors for their occurrence in the metropolis of St. Petersburg at the present time.
Materials and methods. A total of 80 samples taken from morphologically confirmed tissues of oropharyngeal and anal cancer, malignant tumors of vulva, vagina, cervix and cervical intraepithelial neoplasia were studied in St. Petersburg Clinical Oncologic Center. Detection, quantification and genotyping of HPV DNA were carried out by real-time PCR at the St. Petersburg Pasteur Institute.
Results. HPV was detected in 89.7% (61/68) of patients with malignant tumors and 83.3% (10/12) — with severe cervical dysplasia. The vast majority (85.9%) of HPV-positive patients were infected with HPV genotype 16; papillomavirus mixed infection (genotypes 16, 18, 31, 33, 35, 39, 45) was detected in anal cancer, cancer and severe cervical dysplasia. The average viral load in stages III–IV of anal cancer, cervical cancer and severe cervical dysplasia exceeded 5.7 lg HPV DNA/105 cells. Among patients with oropharyngeal cancer, men predominated (85.7%); anal cancer was detected in women (90.0%). No statistically significant risk factors (smoking and alcohol consumption) for the occurrence of HPV-associated malignancies were identified.
Conclusions. The detection of HPV, mainly of genotype 16, varied depending on the location of the neoplasia: anal cancer — 100%, cancer of the female genitalia — 94% (in case of cancer of the vagina and cervix — 100.0%), head and neck cancer — 76.2%. The highest HPV DNA load in the tumor tissue was found in III–IV stages of the cervical and anal cancer.
oai:iimmun.ru:article/122
2022-04-12T21:21:39Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/122
2022-04-12T21:21:39Z
Russian Journal of Infection and Immunity
Vol 3, No 1 (2013); 43-48
NATURAL MUTATION IN THE GENE OF RESPONSE REGULATOR BgrR RESULTING IN REPRESSION OF Bac PROTEIN SYNTHESIS, A PATHOGENICITY FACTOR OF STREPTOCOCCUS AGALACTIAE
Rozhdestvenskaya A.S.; ФГБУ НИИ экспериментальной медицины СЗО РАМН, Санкт-Петербург
Santos-Sanches I.; Центр микробиологии, Университет «Нова», Лиссабон
Dmitriev A.V.; ФГБУ НИИ экспериментальной медицины СЗО РАМН, Санкт-Петербург
2014-07-07 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/122
Streptococcus agalactiae; двухкомпонентная регуляторная система BgrRS; белок Bac; вирулентность
ru
Abstract. Streptococcus agalactiae can cause variety of diseases of newborns and adults. For successful colonization of different human tissues and organs as well as for suppression of the host immune system S. agalactiae expresses numerous virulence factors. For coordinated expression of the virulence genes S. agalactiae employs regulatory molecules including regulatory proteins of two-component systems. Results of the present study demonstrated that in S. agalactiae strain A49V the natural mutation in the brgR gene encoding for BgrR regulatory protein, which is component of regulatory system BgrRS, resulted in the repression of Bac protein synthesis, a virulence factor of S. agalactiae. A single nucleotide deletion in the bgrR gene has caused a shift of the reading frame and the changes in the primary, secondary and tertiary structures of the BgrR protein. The loss of functional activity of BgrR protein in A49V strain and repression of Bac protein synthesis have increased virulence of the strain in experimental animal streptococcal infection.
oai:iimmun.ru:article/2049
2023-04-01T16:28:57Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/2049
2023-04-01T16:28:57Z
Russian Journal of Infection and Immunity
Vol 13, No 1 (2023); 67-74
Dynamics in maturation of SARS-CoV-2 RBD-specific IgG antibody avidity depending on immunization timeframe and type
https://iimmun.ru/iimm/article/download/2049/14616
https://iimmun.ru/iimm/article/download/2049/14617
https://iimmun.ru/iimm/article/download/2049/14618
https://iimmun.ru/iimm/article/download/2049/14619
https://iimmun.ru/iimm/article/download/2049/14620
https://iimmun.ru/iimm/article/download/2049/14621
https://iimmun.ru/iimm/article/download/2049/14623
https://iimmun.ru/iimm/article/download/2049/14624
https://iimmun.ru/iimm/article/download/2049/14625
https://iimmun.ru/iimm/article/download/2049/14626
https://iimmun.ru/iimm/article/download/2049/14627
https://iimmun.ru/iimm/article/download/2049/14628
https://iimmun.ru/iimm/article/download/2049/14629
https://iimmun.ru/iimm/article/download/2049/14630
https://iimmun.ru/iimm/article/download/2049/50408
https://iimmun.ru/iimm/article/download/2049/74061
https://iimmun.ru/iimm/article/download/2049/74062
https://iimmun.ru/iimm/article/download/2049/74063
https://iimmun.ru/iimm/article/download/2049/74064
Kudryashova A.M.; Mechnikov Research Institute of Vaccines and Sera
Manuylov V.A.; Gamaleya National Research Center for Epidemiology and Microbiology of the Ministry of health of Russian Federation
Murzina A.A.; Mechnikov Research Institute of Vaccines and Sera
Kaira A.N.; Mechnikov Research Institute of Vaccines and Sera
Borisova O.V.; Mechnikov Research Institute of Vaccines and Sera
2023-04-01 18:41:17
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/2049
COVID-19; авидность; IgG-антитела; матурация; сыворотки крови человека; ИФА
Array
ru
The aim is to examine dynamics of avidity maturation of IgG antibodies against SARS-CoV-2 RBD depending on the type of immunization (vaccination or infection), as well as on the duration and frequency of immunization. Materials and methods. The study was performed on two sample cohorts collected at two time points during COVID-19 pandemic. The first cohort (group No. 1) consisted of 87 samples of blood sera obtained from COVID-19 convalescents in the period from March to September 2020. The second cohort included 204 samples obtained in September 2021 from two patient groups. Group No. 2 (n = 64) — patients immunized with a full course of Gam-Covid-Vac, group No. 3 (n = 140) — COVID-19 convalescent patients and subjects vaccinated with Gam-Covid-Vac (“hybrid immunity”). Results and conclusion. The dynamics of avidity maturation for SARS-CoV-2 RBD IgG antibodies depending on the method and frequency of immunization, showed that the most effective immunity was formed in COVID-19 convalescent patients and subjects vaccinated with a full course of Gam-Covid-Vac. The “hybrid” immunity showed not only a significantly higher (compared with groups No. 1 and No. 2) level of IgG antibodies (median 228 BAU/ml vs 75 or 119 BAU/ml, p < 0.001), but also a higher level of avidity (IA 90.5% vs 54.5 and 76.6, respectively, p < 0.001, 4M urea). In the test for assessing the avidity index with the denaturing agent 8M urea in patients with “hybrid immunity”, the median level of IA was 25% versus 14.8% and 16% in COVID-19 convalescents and vaccinated subjects (p < 0.001), only in 8 patients IA was higher than 50%. While comparing a single infection of COVID-19 with a full course of Gam-Covid-Vac, it was shown that vaccination leads to higher IgG levels (median values in groups 119 and 75 BAU/ml, p < 0.001) and to a higher avidity index (median 76.6% vs 54.5%). Thus, the more rapid induction of high-avidity antibodies was in vaccinated individuals at early stages of immunization (up to 4 months), during the period when IgG avidity maturation has not yet been completed. Our results showed that during this period vaccination leads to production of antibodies with avidity index at median level of 82% versus 36% in COVID-19 convalescents at similar time point.
oai:iimmun.ru:article/248
2016-07-05T07:39:25Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/248
2016-07-05T07:39:25Z
Russian Journal of Infection and Immunity
Vol 4, No 4 (2014); 365-374
INFLUENZA AND ARVI IN KYRGYZSTAN
Nurmatov Z.S.; Ministry of Health of Kyrgyz Republic, Bishkek, Kyrgyz Republic 720000, The Kyrgyz Republic, Bishkek, Logvynenko str., 8. Phone: +996 (312) 32-00-93; +996 550 10-04-77 (mobile).
2015-02-03 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/248
грипп; острые респираторные вирусные инфекции; Кыргызская Республика
ru
Incidences of influenza and acute respiratory viral infection (ARVI) in the Kyrgyz Republic were analyzed taking in account results of virological testing, routine and sentinel surveillance data. A behavioral study in a population group selected with the method of systematic sampling (N = 226) using a special questionnaire has been conducted. In the period of 2008–2013 3750 ARVI patients were tested by molecular-genetical methods for influenza virus and303 patients also were tested for parainfluenza, rhino-, denoviruses, respiratory-syncytial virus, metapneumovirus and bocavirus. ARVI incidence rate in the epidemic season 2012–2013 in sentinel sites were 2.8 times higher than routine surveillance data for the same period. The results of the behavioral study demonstrated that 34.5% of the population had influenza in the epidemic season while according to routine surveillance data the same rate was only 4.8%. In addition, only 15.5% of the population who had influenza symptoms asked medical aid. It was found that increase in incidencein November 2009 was caused by influenza virus А (H1N1)pdm.
oai:iimmun.ru:article/400
2022-04-12T15:18:27Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/400
2022-04-12T15:18:27Z
Russian Journal of Infection and Immunity
Vol 6, No 2 (2016); 151-156
INDICES OF IMMUNE RESPONSE IN PATIENTS OF FALCIPARUM MALARIA IN REPUBLIC OF GUINEA
Boiro M.Y.; Pasteur Institute of Guinea
Boumbali S.; Pasteur Institute of Guinea
Konstantinov O.K.; Pasteur Institute of Guinea
Kalivogui S.; Pasteur Institute of Guinea
Koulibali M.; International Research Center of Tropical Infections
Bah A.S.; Pasteur Institute of Guinea
2016-06-24 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/400
тропическая малярия;Plasmodium falciparum;антитела;иммуноглобулины;Гвинея
ru
Malaria in the Republic of Guinea is the main cause of morbidity and lethality. It takes the first place in number of all visits in medical service (30–40%) and is the main cause of hospital death. One records annually more than 8 millions malaria cases, and about 60 000 children deaths. Results of study of immune response changing on different disease phases in treatment of autochthon population and immune status of Europeans are presented. It was shown that immunity status (cellular and humoral) in population of Guinea (an endemic country on falciparum malaria) differs from one in Europeans living in tropics. During light forms of malaria one records an increase of T-lymphocyte and IgG number, whereas in grave cases one observed the acute decrease of these indices. The essential increase of B-lymphocyte number does not depends from gravity of disease and from malaria treatment. It was established that appearance of LSA1-41 antibodies was in a more degree in adult patients than in children. The positive correlation between IgM and IgG was established in adult patients as in children.
oai:iimmun.ru:article/1764
2022-04-12T15:58:51Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/1764
2022-04-12T15:58:51Z
Russian Journal of Infection and Immunity
Vol 11, No 4 (2021); 692-700
Hepatitis E virus seroprevalence in indigenous residents of the Hà Giang northern province of Vietnam
https://iimmun.ru/iimm/article/download/1764/11283
https://iimmun.ru/iimm/article/download/1764/11284
https://iimmun.ru/iimm/article/download/1764/11285
https://iimmun.ru/iimm/article/download/1764/11286
https://iimmun.ru/iimm/article/download/1764/11287
https://iimmun.ru/iimm/article/download/1764/11288
https://iimmun.ru/iimm/article/download/1764/11289
https://iimmun.ru/iimm/article/download/1764/11290
https://iimmun.ru/iimm/article/download/1764/11291
https://iimmun.ru/iimm/article/download/1764/11292
https://iimmun.ru/iimm/article/download/1764/11293
https://iimmun.ru/iimm/article/download/1764/11294
https://iimmun.ru/iimm/article/download/1764/11295
https://iimmun.ru/iimm/article/download/1764/11296
https://iimmun.ru/iimm/article/download/1764/11297
https://iimmun.ru/iimm/article/download/1764/11343
https://iimmun.ru/iimm/article/download/1764/11344
https://iimmun.ru/iimm/article/download/1764/11404
Lichnaia E.V.; St. Petersburg Pasteur Institute
Pham T.G.; Joint Russian-Vietnamese Tropical Science and Technology Center
Petrova O.A.; St. Petersburg Pasteur Institute
Tran T.N.; Joint Russian-Vietnamese Tropical Science and Technology Center
Nguyen T.T.; Ha Giang CDC
Bui T.N.; Joint Russian-Vietnamese Tropical Science and Technology Center
Vo V.C.; Joint Russian-Vietnamese Tropical Science and Technology Center
Dmitriev A.V.; Institute of Experimental Medicine
Kalinina O.V.; St. Petersburg Pasteur Institute
2021-07-15 00:00:00
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1764
гепатит Е; анти-HEV IgG; серопревалентность; провинция Ха Занг; Вьетнам; этнические группы
Исследование выполнено в рамках программы российско-вьетнамского сотрудничества, направленной на реализацию Распоряжения Правительства Российской Федерации от 13.07.2019 г. № 1536-р за счет бюджетных ассигнований на оказание научно-методичес кой и материально-технической поддержки Социалистической Республике Вьетнам.
ru
Vietnam is an endemic territory of the South-East Asia in terms of the prevalence of hepatitis E virus. However, the data on the prevalence of HEV infection among the indigenous population of Vietnam are limited, whereas the data on various minor ethnic groups are not available. Рopulation of the Northern province Hà Giang is characterized by ethnic diversity and consists of about 22 ethnic groups that have preserved their ethnic identity determining the features of their lifestyle and farming. The goal of the current study was to conduct a cross-sectional examination to assess prevalence of serological markers of HEV infection in the indigenous population of the Northern province of Vietnam, Hà Giang. Materials and methods. A total of 1127 healthy indigenous residents aged 18 to 83 years (average age 42.8±1.5) who lived in three regions (Yên Minh, Bắc Mê and Đông Văn) of Hà Giang province in 2019 were enrolled in the study. The presence of HEV-specific IgG antibodies (anti-HEV IgG) was determined by the ELISA using kits “DS-IFA-ANTI-HEV-G” (LLC NPO “Diagnostic Systems”, Russia). Results. The prevalence of anti-HEV IgG antibodies in the indigenous population in the Hà Giang province was 74.4% (838/1127; 95% CI 71.7–76.8), with the peak occurrence of anti-HEV IgG (87.6%) found in Đông Văn region, which was significantly higher compared to those in the Bắc Mê (χ2 = 16.37, p = 0.000052) and Yên Minh (χ2 = 214.64, p < 0.00001) regions. The Yên Minh Region was characterized by the lowest percentage of subjects involved in the epidemic process (χ2 = 77.55, p < 0.00001). No significant gender-related differences were detected in antiHEV IgG level both in general and in individual regions. The peak frequency of anti-HEV IgG (85.9%) was found in the H’mong ethnic group, which was significantly higher than in the Tay ethnic groups (χ2 = 77.32, p < 0.00001) and in the remaining minor ethnic groups (χ2 = 63.44, p < 0.00001). Conclusion. The results of this study indicate a high seroprevalence of hepatitis E virus in the minor ethnic groups, which preserve the national lifestyle in the Hà Giang province of the Northern Vietnam. As the Hà Giang province is located in remote mountain areas, a number of which are still difficult to access, its low economic status, poor sanitary and hygienic living conditions, lack of high-quality water supply, multinational population following different lifestyles, the contact of population with natural potential sources of HEV infection, including various wild and domestic animals, contribute to the successful spread of the hepatitis E virus in the region and the involvement of all populational age groups in the epidemic process. Taking into account the rapid development of the tourism industry in the Hà Giang Province particularly in the Đông Văn Region, where the Đông Văn Karst Plateau Geopark being recognized by UNESCO as national treasure is located, the results of this study emphasize a need to plan and perform the events aimed for preventing and monitoring HEV infection in endemic regions in Vietnam in order to reduce a risk of spread of hepatitis E virus not only inside, but also outside the country.
oai:iimmun.ru:article/1716
2023-11-19T09:12:09Z
iimm:ORIG
v2
https://iimmun.ru/iimm/article/view/1716
2023-11-19T09:12:09Z
Russian Journal of Infection and Immunity
Vol 12, No 2 (2022); 323-330
Characteristics of cellular and humoral immunity parameters in patients with mechanical jaundice, caused by cholangiocarcinoma
https://iimmun.ru/iimm/article/download/1716/10721
https://iimmun.ru/iimm/article/download/1716/10722
https://iimmun.ru/iimm/article/download/1716/10723
https://iimmun.ru/iimm/article/download/1716/10724
https://iimmun.ru/iimm/article/download/1716/10725
https://iimmun.ru/iimm/article/download/1716/10726
https://iimmun.ru/iimm/article/download/1716/10727
https://iimmun.ru/iimm/article/download/1716/10728
https://iimmun.ru/iimm/article/download/1716/12762
Smirnova O.V.; Scientific Research Institute of Medical Problems of the North, Federal Research Center Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences
Gubanov .G.; Scientific Research Institute of Medical Problems of the North, Federal Research Center Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences
Kasparov E.V.; Scientific Research Institute of Medical Problems of the North, Federal Research Center Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences
Darenskaya M.A.; Scientific Сentre for Family Health and Human Reproduction Problems
Kolesnikova L.I.; Scientific Сentre for Family Health and Human Reproduction Problems
Kolesnikov S.N.; Scientific Сentre for Family Health and Human Reproduction Problems
2022-05-13 14:39:24
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
url:https://iimmun.ru/iimm/article/view/1716
клеточный иммунитет; гуморальный иммунитет; механическая желтуха; холангиокарцинома; адаптивный иммунитет; лимфоциты; иммуноглобулины; механическая желтуха злокачественного генеза
Array
ru
In the modern world, obstructive jaundice caused by developing a malignant disease — cholangiocarcinoma, poses a significant medical and social problem in economically developed and in developing countries. The aim of this study was to examine parameters of cellular and humoral immunity in patients with obstructive jaundice caused by cholangiocarcinoma before and after surgical intervention. There were enrolled 56 patients with obstructive jaundice caused by cholangiocarcinoma (stages T2-3N0-1M0) and 90 age-matched apparently healthy volunteers. Cellular immunity parameters were studied by using an FC500 flow cytometer (Beckman Coulter, USA). The parameters of humoral immunity were assessed by measuring level of serum immunoglobulins A, M, G, E by using enzyme-linked immunosorbent assay kits manufactured by Vector-Best (Russia). The results were statistically analyzed using the Statistica v. 12.0 software (StatSoft Inc., USA). According to the study results, in patients with obstructive jaundice caused by cholangiocarcinoma, there was revealed activation of humoral immunity due to increased serum level of IgA, IgG, IgE with a profoundly depressed some arms of cellular immunity contributing to the imbalanced work of the entire immune system. The imbalance in the cellular arm of immune system was manifested by decreased count of T helper and increased cytotoxic T cell subsets as well as decline in the CD4+/CD8+ T cell ratio. An increased count of cytotoxic T lymphocytes points at lowered T cell immunity and existence of a cytotoxic effect at the cellular level. A decrease in the count of pan-marker-positive T lymphocytes indicates about generally reduced activity of the T cell arm due to altered T cell function and, as a result, affected antigen-presenting function in immune cells. The development of T cell immunodeficiency due to T-cell apoptosis confirms upregulated expression of immunomarkers CD38+, CD95+. An increased serum level for some immunoglobulins suggests developing imbalance in the humoral immunity, whereas rise in the circulating immune complexes indicates about pronounced body intoxication caused by obstructive jaundice due to hyperbilirubinemia and disintegration of tumor cells. Application of surgical correction of obstructive jaundice contributed to normalizing parameters of cellular and humoral immunity, resulting in immunocorrective effect.
8741ed17776208db83dbf1800f5ed641